Vascular-targeted photodynamic therapy(V-PDT)is an effective treatment for port wine stains(PWS).However,repeated treatment is usually needed to achieve optimal treatment outcomes,possibly due to the limited treatment...Vascular-targeted photodynamic therapy(V-PDT)is an effective treatment for port wine stains(PWS).However,repeated treatment is usually needed to achieve optimal treatment outcomes,possibly due to the limited treatment light penetration depth in the PWS lesion.The optical clearing technique can increase light penetration in depth by reducing light scattering.This study aimed to investigate the V-PDT in combination with an optical clearing agent(OCA)for the therapeutic enhancement of V-PDT in the rodent skinfold window chamber model.Vascular responses were closely monitored with laser speckle contrast imaging(LSCI),optical coherence tomography angiography,and stereo microscope before,during,and after the treatment.We further quantitatively demonstrated the effects of V-PDT in combination with OCA on the blood flow and blood vessel size of skin microvasculature.The combination of OCA and V-PDT resulted in significant vascular damage,including vasoconstriction and the reduction of blood flow.Our results indicate the promising potential of OCA for enhancing V-PDT for treating vascular-related diseases,including PWS.展开更多
Doppler optical coherence tomography or optical Doppler tomography （ODT） has been demonstrated to spatially localize flow velocity mapping as well as to obtain images of microstructure of samples simulta- neously. I...Doppler optical coherence tomography or optical Doppler tomography （ODT） has been demonstrated to spatially localize flow velocity mapping as well as to obtain images of microstructure of samples simulta- neously. In recent decades, spectral domain Doppler optical coherence tomography （OCT） has been applied to observe three-dimensional （3D） vascular distribution. In this study, we developed a spectral domain optical coherence tomography system （SD-OCT） using super luminescent diode （SLD） as light source. The center wavelength of SLD is 835 nm with a 45-nm bandwidth. Theoretically, the transverse resolution, axial resolution and penetration depth of this SD-OCT system are 6.13 μm, 6.84 μm and 3.62 mm, respectively. By imaging mouse model with dorsal skin window chamber, we obtained a series of real-time OCT images and reconstructed 3D images of the specific area inside the dorsal skin window chamber by Amira. As a result, we can obtain the clear and complex distribution images of blood vessels of mouse model.展开更多
基金supported by the National Natural Science Foundation of China(Grant Numbers 62205025 and 61835015)Beijing Natural Science Foundation(7222309)+2 种基金the Open Project Program of Wuhan National Laboratory for Optoelectronics(2020WNLOKF025)CAMS Innovation Fund for Medical Sciences(CIFMS)(2019-I2M-5-061)Beijing Institute of Technology Research Fund Program for Young Scholars(XSQD-202123001).
文摘Vascular-targeted photodynamic therapy(V-PDT)is an effective treatment for port wine stains(PWS).However,repeated treatment is usually needed to achieve optimal treatment outcomes,possibly due to the limited treatment light penetration depth in the PWS lesion.The optical clearing technique can increase light penetration in depth by reducing light scattering.This study aimed to investigate the V-PDT in combination with an optical clearing agent(OCA)for the therapeutic enhancement of V-PDT in the rodent skinfold window chamber model.Vascular responses were closely monitored with laser speckle contrast imaging(LSCI),optical coherence tomography angiography,and stereo microscope before,during,and after the treatment.We further quantitatively demonstrated the effects of V-PDT in combination with OCA on the blood flow and blood vessel size of skin microvasculature.The combination of OCA and V-PDT resulted in significant vascular damage,including vasoconstriction and the reduction of blood flow.Our results indicate the promising potential of OCA for enhancing V-PDT for treating vascular-related diseases,including PWS.
文摘Doppler optical coherence tomography or optical Doppler tomography （ODT） has been demonstrated to spatially localize flow velocity mapping as well as to obtain images of microstructure of samples simulta- neously. In recent decades, spectral domain Doppler optical coherence tomography （OCT） has been applied to observe three-dimensional （3D） vascular distribution. In this study, we developed a spectral domain optical coherence tomography system （SD-OCT） using super luminescent diode （SLD） as light source. The center wavelength of SLD is 835 nm with a 45-nm bandwidth. Theoretically, the transverse resolution, axial resolution and penetration depth of this SD-OCT system are 6.13 μm, 6.84 μm and 3.62 mm, respectively. By imaging mouse model with dorsal skin window chamber, we obtained a series of real-time OCT images and reconstructed 3D images of the specific area inside the dorsal skin window chamber by Amira. As a result, we can obtain the clear and complex distribution images of blood vessels of mouse model.
