The struetural effect of the conjugative system(C)with carbonyl-imino bridges has been studied.The results show that:In the conjugated system(C),there is no electronic absorption peak attributable to the whole system,...The struetural effect of the conjugative system(C)with carbonyl-imino bridges has been studied.The results show that:In the conjugated system(C),there is no electronic absorption peak attributable to the whole system,but there are three π-π* bands each nt which displays chacactecistics of its own independently.These indicate that the two bridges-carbonyl-and-imino-can block the tlanSmlSSion of the conjngative polarization of the whole system,so as to form three segments,this is verified by means of chemical synthesis and degradation.展开更多
Exosomes are promising biological biomarkers for monitoring a number of diseases, especially cancers. Here, we developed a double gold nanoparticles （GNPs） conjugates based lateral flow assay （D-LFA） for rapidly a...Exosomes are promising biological biomarkers for monitoring a number of diseases, especially cancers. Here, we developed a double gold nanoparticles （GNPs） conjugates based lateral flow assay （D-LFA） for rapidly and sensitively detecting and molecular profiling of exosomes. Based on these two GNPs conjugates, the signal amplification can be achieved without any additional operation. The antibody on the 1st GNPs conjugate could recognize exosomes and form a sandwich format on the test zone. The 2nd GNPs conjugate was designed to bind to the 1st GNPs conjugate to realize signal amplification. This biosensor enabled visual and quantitative detection of exosomes by the accumulation of GNPs on the test zone and showed a low detection limit of 1.3x10^3 particles/laL, which has been improved 13-fold compared with the normal lateral flow assay. The D-LFA exhibited good sensitivity and reproducibility and has been successfully used for the detection ofexosomes in fetal bovine serum, which proved its potential application in practical diagnostics.展开更多
An efficient synthesis of 3-(diarylallylidene)oxindoles from 3-(1 H-indol-3-yl)-1,1-diarylpropan-1-ol under metal-free conditions is described.NBS serves as a critical medium leading to a facile oxygenmigration in the...An efficient synthesis of 3-(diarylallylidene)oxindoles from 3-(1 H-indol-3-yl)-1,1-diarylpropan-1-ol under metal-free conditions is described.NBS serves as a critical medium leading to a facile oxygenmigration in the transformation.The protocol has advantages of high efficiency,simple opreation,mild reaction conditions,good atom-economy,wide substrate scope and good yields of products.A detailed mechanism is proposed after careful investigation.展开更多
文摘The struetural effect of the conjugative system(C)with carbonyl-imino bridges has been studied.The results show that:In the conjugated system(C),there is no electronic absorption peak attributable to the whole system,but there are three π-π* bands each nt which displays chacactecistics of its own independently.These indicate that the two bridges-carbonyl-and-imino-can block the tlanSmlSSion of the conjngative polarization of the whole system,so as to form three segments,this is verified by means of chemical synthesis and degradation.
基金supported by the National Natural Science Foundation of China (21475009, 21475008)Beijing Municipal Science & Technology Commission (Z161100000116037)
文摘Exosomes are promising biological biomarkers for monitoring a number of diseases, especially cancers. Here, we developed a double gold nanoparticles （GNPs） conjugates based lateral flow assay （D-LFA） for rapidly and sensitively detecting and molecular profiling of exosomes. Based on these two GNPs conjugates, the signal amplification can be achieved without any additional operation. The antibody on the 1st GNPs conjugate could recognize exosomes and form a sandwich format on the test zone. The 2nd GNPs conjugate was designed to bind to the 1st GNPs conjugate to realize signal amplification. This biosensor enabled visual and quantitative detection of exosomes by the accumulation of GNPs on the test zone and showed a low detection limit of 1.3x10^3 particles/laL, which has been improved 13-fold compared with the normal lateral flow assay. The D-LFA exhibited good sensitivity and reproducibility and has been successfully used for the detection ofexosomes in fetal bovine serum, which proved its potential application in practical diagnostics.
基金the National Natural Science Foundation of China(Nos.21772138 and 21672157)PAPD,the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX19_1899)+1 种基金the Project of Scientific and Technologic Infrastructute of Suzhou(No.SZS201708)State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials for financial support。
文摘An efficient synthesis of 3-(diarylallylidene)oxindoles from 3-(1 H-indol-3-yl)-1,1-diarylpropan-1-ol under metal-free conditions is described.NBS serves as a critical medium leading to a facile oxygenmigration in the transformation.The protocol has advantages of high efficiency,simple opreation,mild reaction conditions,good atom-economy,wide substrate scope and good yields of products.A detailed mechanism is proposed after careful investigation.
