为研究不同草田轮作模式下土壤养分及细菌群落的组成特征,以5年紫花苜蓿-1年小麦(A5W1)、5年紫花苜蓿-1年玉米(A5C1)、5年紫花苜蓿-2年小麦(A5W2)和5年紫花苜蓿-2年玉米(A5C2)草田轮作模式为对象,测定了土壤有机质、全氮、全磷、全钾、...为研究不同草田轮作模式下土壤养分及细菌群落的组成特征,以5年紫花苜蓿-1年小麦(A5W1)、5年紫花苜蓿-1年玉米(A5C1)、5年紫花苜蓿-2年小麦(A5W2)和5年紫花苜蓿-2年玉米(A5C2)草田轮作模式为对象,测定了土壤有机质、全氮、全磷、全钾、碱解氮、速效磷和速效钾含量,在此基础上,基于16S rRNA基因序列扩增子测序研究了4种轮作模式下耕层土壤细菌群落组成特征。结果表明:轮作第2年各土壤养分含量较第1年显著下降。细菌组成研究结果显示,4种轮作模式下占优势的菌门为变形菌门、放线菌门和厚壁菌门。土壤细菌群落优势属以丙酸杆菌属(Propionibacterium)、芽孢杆菌属(Bacillus)、链球菌属(Streptococcus)以及奈瑟氏球菌属(Neisseria)为主。土壤细菌多样性以A5C2轮作处理最高,A5C1最低;聚类分析显示A5W1和A5C1的组成最接近,其次是A5C2,A5W2的组成与前两者相差较大。土壤养分含量与几种优势属之间呈显著相关性(P<0.05 or P<0.01)。研究结果揭示了河西走廊灌溉区种植紫花苜蓿多年后轮作小麦和玉米改善土壤肥力和防治某些土传植物病害微生物的机理。展开更多
In the continuous annual wheat-corn cropping area of North China Plain,no-till planting that promotes soil conservation and crop yield while reducing operation cost has been gradually accepted by local farmers.However...In the continuous annual wheat-corn cropping area of North China Plain,no-till planting that promotes soil conservation and crop yield while reducing operation cost has been gradually accepted by local farmers.However,previous wheat residue is the main limiting factor affecting the performance of existing planters in placing seeds at uniform spacing and optimum depth in residue covered fields.In order to solve this problem,a kind of ground-wheel-driven row cleaner was designed,developed and mounted on row units of a four-row pneumatic precision planter.The planter has two adjacent row units equipped with the newly designed row cleaners and the other two adjacent row units equipped with the commonly used inactive row cleaners.This was used for planting at three forward speeds(4 km/h,6 km/h and 8 km/h)into half residue(HR)and whole residue(WR)plots.The amount of residue removal,seeding depth,emergence rate and indices of uniformity in seed spacing(missing-seeding index,quality of feeding index and precision index)were measured.The newly designed row cleaner performed better with regard to residue removal,with the average percentage of residue cleared as 63.0%compared to 40.3%for the inactive row cleaner.For the HR and WR plots,percentage of residue cleared of the newly designed row cleaner reached 57.1%and 68.9%respectively,suggesting that the newly designed row cleaner can work more effectively at high residue level.By contrast,with the percentage of residue cleared of the inactive row cleaner as 43.1%and 37.5%in HR and WR plots,suggesting that the inactive row cleaner just can work effectively under low residue condition.Values of missing-seeding index,QFI,precision index,coefficient of variation of depth and percent emergence for the newly designed row cleaner under whole residue level are comparable to those for the inactive row cleaner under half residue level.The result indicates that the effect of using the newly designed row cleaner is equal to that of reducing surface residue,and can help to maintain the uniformity of seed spacing and seeding depth.The newly designed row cleaner generally performed better at forward speed of 6 km/h,based on the distribution of seeds along rows and seeding depth uniformity.展开更多
文摘为研究不同草田轮作模式下土壤养分及细菌群落的组成特征,以5年紫花苜蓿-1年小麦(A5W1)、5年紫花苜蓿-1年玉米(A5C1)、5年紫花苜蓿-2年小麦(A5W2)和5年紫花苜蓿-2年玉米(A5C2)草田轮作模式为对象,测定了土壤有机质、全氮、全磷、全钾、碱解氮、速效磷和速效钾含量,在此基础上,基于16S rRNA基因序列扩增子测序研究了4种轮作模式下耕层土壤细菌群落组成特征。结果表明:轮作第2年各土壤养分含量较第1年显著下降。细菌组成研究结果显示,4种轮作模式下占优势的菌门为变形菌门、放线菌门和厚壁菌门。土壤细菌群落优势属以丙酸杆菌属(Propionibacterium)、芽孢杆菌属(Bacillus)、链球菌属(Streptococcus)以及奈瑟氏球菌属(Neisseria)为主。土壤细菌多样性以A5C2轮作处理最高,A5C1最低;聚类分析显示A5W1和A5C1的组成最接近,其次是A5C2,A5W2的组成与前两者相差较大。土壤养分含量与几种优势属之间呈显著相关性(P<0.05 or P<0.01)。研究结果揭示了河西走廊灌溉区种植紫花苜蓿多年后轮作小麦和玉米改善土壤肥力和防治某些土传植物病害微生物的机理。
基金This study was supported by the National Science and Technology Research Program(2013BAD08B01-3)the Soil-Machine-Plant Key Laboratory of Ministry of Agriculture of PR China.
文摘In the continuous annual wheat-corn cropping area of North China Plain,no-till planting that promotes soil conservation and crop yield while reducing operation cost has been gradually accepted by local farmers.However,previous wheat residue is the main limiting factor affecting the performance of existing planters in placing seeds at uniform spacing and optimum depth in residue covered fields.In order to solve this problem,a kind of ground-wheel-driven row cleaner was designed,developed and mounted on row units of a four-row pneumatic precision planter.The planter has two adjacent row units equipped with the newly designed row cleaners and the other two adjacent row units equipped with the commonly used inactive row cleaners.This was used for planting at three forward speeds(4 km/h,6 km/h and 8 km/h)into half residue(HR)and whole residue(WR)plots.The amount of residue removal,seeding depth,emergence rate and indices of uniformity in seed spacing(missing-seeding index,quality of feeding index and precision index)were measured.The newly designed row cleaner performed better with regard to residue removal,with the average percentage of residue cleared as 63.0%compared to 40.3%for the inactive row cleaner.For the HR and WR plots,percentage of residue cleared of the newly designed row cleaner reached 57.1%and 68.9%respectively,suggesting that the newly designed row cleaner can work more effectively at high residue level.By contrast,with the percentage of residue cleared of the inactive row cleaner as 43.1%and 37.5%in HR and WR plots,suggesting that the inactive row cleaner just can work effectively under low residue condition.Values of missing-seeding index,QFI,precision index,coefficient of variation of depth and percent emergence for the newly designed row cleaner under whole residue level are comparable to those for the inactive row cleaner under half residue level.The result indicates that the effect of using the newly designed row cleaner is equal to that of reducing surface residue,and can help to maintain the uniformity of seed spacing and seeding depth.The newly designed row cleaner generally performed better at forward speed of 6 km/h,based on the distribution of seeds along rows and seeding depth uniformity.