Peripheral CD4^(+)CD8^(+) double positive T cells:A potential marker to evaluate renal impairment susceptibility during systemic lupus erythematosus

Lupus nephritis(LN) has a high incidence in systemic lupus erythematosus(SLE) patients, but there is a lack of sensitive predictive markers. The purpose of the study was to investigate the association between the CD4^(+)CD8^(+)double positive T(DPT) lymphocytes and LN. The study included patients with SLE without renal impairment(SLE-NRI), LN, nephritic syndrome(NS), or nephritis. Peripheral blood lymphocyte subsets were analyzed by flow cytometry. Biochemical measurements were performed with peripheral blood in accordance with the recommendations proposed by the National Center for Clinical Laboratories. The proportions of DPT cells in the LN group were significantly higher than that in the SLE-NRI group(t=4.012, P<0.001), NS group(t=3.240,P=0.001), and nephritis group(t=2.57, P=0.011). In the LN group, the risk of renal impairment increased significantly in a DPT cells proportion-dependent manner. The risk of LN was 5.136 times(95% confidence interval, 2.115–12.473) higher in cases with a high proportion of DPT cells than those whose proportion of DPT cells within the normal range. These findings indicated that the proportion of DPT cells could be a potential marker to evaluate LN susceptibility, and the interference of NS and nephritis could be effectively excluded when assessing the risk of renal impairment during SLE with DPT cell proportion.

Accurate design of spatially separated double active site in Bi_(4)NbO_(8)Cl single crystal to promote Z-Scheme photocatalytic overall water splitting

The efficiency of photocatalytic overall water splitting was mainly limited by the slow reaction kinetics of water oxidation.How to design effective surface active site to overcome the slow water oxidation reaction was a major challenge.Here,we propose a strategy to accelerate surface water oxidation through the fabrication spatially separated double active sites.FeCoPi/Bi_(4)NbO_(8)Cl-OVs photocatalyst with spatially separated double active site was prepared by hydrogen reduction photoanode deposition method.Due to the high matching of the spatial loading positions of FeCoPi and OVs with the photogenerated charge distribution of Bi_(4)NbO_(8)Cl and corresponding reaction mechanisms of substrate,the FeCoPi and OVs on the(001)and(010)crystal planes of Bi_(4)NbO_(8)Cl photocatalyst provided surface active site for water oxidation reaction and electron shuttle reaction(Fe^(3+)/Fe^(2+)),respectively.Under visible light irradiation,the evolution O_(2)rate of FeCoPi/Bi_(4)NbO_(8)Cl OVs was 16.8μmol h^(-1),as 32.9 times as Bi_(4)NbO_(8)Cl.Furthermore,a hydrogen evolution co-catalyst PtRu@Cr_(2)O_(3)was prepared by sequential photodeposition method.Due to the introduction of Ru,the Schottky barrier between PbTiO_(3)and Pt was effectively reduced,which promoted the transfer of photogenerated electrons to PtRu@Cr_(2)O_(3)thermodynamically,the evolution H_(2)rate on PtRu@Cr_(2)O_(3)/PbTiO_(3)increased to 664.8 times.On based of the synchronous enhancement of the water oxidation performance on FeCoPi/Bi_(4)NbO_(8)Cl-OVs and water reduction performance on PtRu@Cr_(2)O_(3)/PbTiO_(3),a novel Z-Scheme photocatalytic overall water splitting system(FeCoPi/Bi_(4)NbO_(8)Cl-OVs)mediated by Fe^(3+)/Fe^(2+)had successfully constructed.Under visible light irradiation,the evolution rates of H_(2)and O_(2)were 2.5 and 1.3μmol h^(-1),respectively.This work can provide some reference for the design of active site and the controllable synthesis of OVs spatial position.On the other hand,the hydrogen evolution co catalyst(PtRu@Cr_(2)O_(3))and the co catalyst FeCoPi for oxygen evolution contributed to the construction of an overall water splitting system.

Development and optimization of a double antibody sandwich ELISA for the detection of goose T cell surface CD8α molecule

CD8, a glycoprotein on the surface of T cells, is involved in the defense against viral infection and plays significant roles in antigen presentation and in the antiviral immune response. CD8 is composed of two chains. Of these, the CD8α chain was chosen for the detection because it involved in both the CD8αα homodimer and the CD8αβ heterodimer. Here, we established a double antibody sandwich enzyme-linked immunosorbent assay（DAS-ELISA） for specific detection of goose CD8α（go CD8α）. The results showed that the optimal coated antibody and antigen dilutions were 1：50（the antibody titer was 1：12 800） and 1：32（0.3 ng m L^–1）, respectively, while the optimal capture antibody and horseradish peroxidase（HRP）-labelled goat anti-rabbit Ig G dilutions were 1：50（the antibody titer was 1：51 200） and 1：4 000（the antibody titer was 1：5 000）, respectively. The optimal blocking buffer was 5% bovine serum albumin（BSA）. The best incubating condition was overnight at 4℃, the best blocking time was 120 min and the best anti-capture antibody working time was 150 min. In addition, the minimum dose detectable by DAS-ELISA was 5×10^–3 ng m L^–1. Most importantly, go CD8α expression levels in goose spleen mononuclear cells（MNCs） post-Goose parvoviruse（GPV） infection were found to be significantly up-regulated using the DAS-ELISA method, which was consistent with previous results obtained using real-time quantitative PCR. In conclusion, the DAS-ELISA method reported here is a novel, specific technique for the clinical detection of go CD8α.

Research on Planar Double Compound Pendulum Based on RK-8 Algorithm

Establishing the Lagrangian equation of double complex pendulum system and obtaining the dynamic differential equation,we can analyze the motion law of double compound pendulum with application of the numerical simulation of RK-8 algorithm.When the double compound pendulum swings at a small angle,the Lagrangian equation can be simplified and the normal solution of the system can be solved.And we can walk further on the relationship between normal frequency and swing frequency of double pendulum.When the external force of normal frequency is applied to the double compound pendulum,the forced vibration of the double compound pendulum will show the characteristics of beats.

Comparison of DNA double-strand breaks induced by ^(16)O^(8+) in deproteinized DNA and intact cells

The yield of DNA double-strand breaks (DSBs) is sure to be influenced by theenvironment around DNA molecule. Inverse pulsed-field gel electrophoresis (PIGE) has beenapplied to compare the sensitivity of B16 cells and their DNA in DSBs induced by 75 MeV/u16O8+ beam. Results show that the percentages of DNA released from the plug(PR) in bothkinds of tile samples increase with the dose and approach a similar quasi-threshold of about81%. A simple new equation was presented to calculate the break level of DNA molecules.Within a certain dose, the relationship between the break level and the dose is linear. Theyield of DSBs in deproteinized DNA was 1.11 DSBs/100 Mbp/Gy, while that in intact cells was0.60DSBs/100Mbp/Gy. It is testified that deproteinized DNA is more sensitive to oxygen ionsirradiation than intact cells.

世爵C8 Double 12 S

与其说世爵的汽车是产品，倒不如说它们是艺术品。首先，现在不可能再有人像他们一样生产工业产品。其次，世爵的每一辆汽车都满载着设计和制造者的感情和心血。

含溴苯基团的8-羟基喹啉衍生物的合成与性质研究

以8羟基喹啉、对溴苯胺为主要原料,经过Reimer-Tiemann反应、席夫碱制备反应,重氮化反应,将溴苯基团分别通过C=N键和N=N键连接到8羟基喹啉上,从而合成了两种扩大π体系的8羟基喹啉衍生物。通过^(1)H NMR、^(13)C NMR,FT-IR以及HR-MS等手段充分表征,并对两种衍生物的各种物理和化学性质进行了深入研究。除此之外,还应用Gaussian程序对两种衍生物的几何构型进行了理论计算。结果显示,分子结构存在细微的差别,可以对8羟基喹啉衍生物的各种性质产生较大的影响。

lncRNA DUXAP8在子宫内膜癌细胞中的作用机制研究

目的探究lncRNA DUXAP8在子宫内膜癌发生发展中的作用。方法使用癌症基因组图谱(TCGA)数据库分析lncRNA DUXAP8在子宫内膜癌组织中的表达水平以及DUXAP8与患者预后的关系,qRT-PCR检测DUXAP8在子宫内膜癌细胞系中的表达情况。通过转染构建了DUXAP8过表达或敲低的HEC-1A细胞系。CCK-8、克隆形成、Transwell、划痕实验和流式细胞术实验分别检测不同转染处理后子宫内膜癌细胞的增殖、侵袭、迁移、周期和凋亡情况,Western blot实验检测上皮细胞间质转化(EMT)相关蛋白的表达水平。结果DUXAP8在子宫内膜癌中显著上调,DUXAP8高表达的子宫内膜癌患者预后相对较差。抑制DUXAP8的表达可以阻碍肿瘤细胞的增殖、侵袭、迁移和EMT进程,细胞周期将阻滞于G0/G1期,并能诱导细胞凋亡的发生;过表达DUXAP8后肿瘤细胞的增殖、侵袭、迁移和EMT进程显著增强,细胞周期阻滞于S期,细胞凋亡水平显著降低。结论DUXAP8在子宫内膜癌中起促癌基因的作用,有望成为子宫内膜癌治疗的新靶标。

面向ARMv8 64位多核处理器的QGEMM设计与实现

该文在ARMv8 64位多核处理器上基于OpenBLAS首次设计、实现并优化了四精度矩阵乘法(Quadruple precision General Matrix-Matrix Multiplication,QGEMM).由于浮点计算中不可避免地引入舍入误差,双精度矩阵乘法(DGEMM)在某些情况下不能给出令人满意的数值结果,因此需要高精度或多精度算法来实现更精确的计算.Double-double算术是一种较为有效和广泛使用的手段.文中采用double-double数据格式构建结构体存储四精度浮点数据;基于OpenBLAS中的稠密矩阵计算的分块算法,增加四精度数据格式的相关的头文件和源文件,并用汇编代码撰写文中所提出的QGEMM的核心内核;利用无误差变换技术,调整并优化内核中的算法流程,避免规格化操作步骤造成的数据强制依赖关系;通过分析算法的数据依赖关系,设计寄存器的分配和轮转策略,优化指令调度顺序,开发指令级并行性,提高QGEMM的实际性能.根据具体算法使用混合乘加指令(FMA)的程度不同,文中采用了算法理论峰值性能这一概念,其有别于机器理论峰值的概念,能更好地评估文中所提出的QGEMM的实际效率.数值实验表明:文中通过汇编代码实现并优化的QGEMM性能最高达到19.7Gflops,效率为在ARMv864位多核处理器平台上QGEMM算法理论峰值性能的82.1%,在满足数值结果精度要求的同时,其计算速度约是由C语言撰写的未优化的QGEMM和MBLAS中QGEMM的5.8倍,是编译器GCC实现的long double数据格式的QGEMM的24倍.同时数值实验还显示文中提出的QGEMM针对不同规模的矩阵具有较好的线程可扩展性.

抗人白细胞介素8单克隆抗体乳膏治疗湿疹的随机双盲对照临床试验

目的：评价抗人白细胞介素8(IL-8)单克隆抗体乳膏治疗湿疹的疗效及安全性。方法：以乳膏基质为对照,对64例湿疹病人进行随机双盲用药,试验组32例,对照组32例,每日早晚各外用1次,连续3 wk,观察其疗效和不良反应。结果：经过3 wk治疗,试验组和对照组痊愈率分别为25%和3%,有效率分别为47%和9%,临床疗效2组差异有显著意义(P＜0.05),试验组优于对照组,2组不良反应发生率相似(P＞0.05)。结论：抗人IL-8单克隆抗体乳膏治疗湿疹安全、有效。

47,XXX/48,XXX,+8合并贝赫切特综合征1例报告及文献复习

目的分析临床罕见的47XXX/48XXX+8合并贝赫切特综合征患者的临床特征和诊断、治疗。方法回顾1例47XXX/48XXX+8合并贝赫切特综合征患者的临床资料,染色体核型分析及基因分析结果,并复习相关文献。结果患儿,女,11岁女性,反复发热6年余并伴有反复口腔溃疡及外阴溃疡,临床诊断为贝赫切特综合征;集合全基因芯片扫描及外周血染色体核型分析结果,患儿染色体核型为为47,XXX[12]/48,XXX,+8[18]。结论染色体核型分析与基因分析在诊断疾病上有着相互补充的作用。8号染色体上可能存在贝赫切特综合征相关致病基因的基因剂量增加效应。

1,8-二羟基蒽醌铅/铜盐的合成及其燃烧催化作用

以1,8-二羟基蒽醌为原料制备了1,8-二羟基蒽醌铅（DHAAPb）和1,8-二羟基蒽醌铜（DHAACu）。采用红外光谱、元素分析及X-荧光对其结构进行了表征。研究了其对双基推进剂和RDX基改性双基推进剂（RDX-CMDB）燃烧性能的影响,结果表明,DHAAPb在低压区（2~6 MPa）能显著提高双基推进剂的燃速,催化效率达到2.0以上;并使双基推进剂在10~16 MPa压力指数降低至0.1;DHAAPb和DHAAPb/DHAACu能使RDX-CMDB 2~6 MPa下燃速提高两倍以上,在6~18 MPa压力范围内,压力指数分别降低至0.29和0.16,且均能呈现平台燃烧。

甘蓝型双低隐性核不育杂交种‘沪油杂8号'的选育

‘沪油杂8号'是利用隐性上位互作核不育三系选育的甘蓝型油菜双低杂交种,具有高产、稳产和抗油菜菌核病和病毒病等特性。在上海市油菜区域试验中,2年平均产量3 094.5 kg/hm^2,较对照品种(2009年‘沪油15',2010年‘沪油杂4号')增产20.12%,产油量比对照增加31.29%。在上海市油菜生产试验中,平均产量3 108.6 kg/hm^2,比对照‘沪油杂4号'增产17.90%,产油量增加22.88%。在国家冬油菜区域试验(长江下游)中,平均产量为2 551.95 kg/hm^2,较对照‘秦油7号'增产8.10%,产油量增加5.69%。种子含油率43.39%,芥酸含量0.5%,硫苷总量26.40μmol/g。

特高压同塔双回交流输电线路采用(8+2)分裂导线与常规导线电晕特性对比

目前特高压同塔双回路采用常规8×630 mm^2导线,由于线路分裂数对高压线路电磁环境参数影响较大,考虑在确保电磁环境满足要求的前提下,进一步减小导线截面提高工程经济性,为此对比(8+2)×400 mm^2、8×630mm^2及10×400 mm^2等常规分裂型式导线的同塔双回路无线电干扰、可听噪声、电晕损失。首先通过有限元方法对(8+2)×400 mm^2方式新增2根子导线优化排列位置进行了研究,再利用各预测方法对各种导线形式进行了全面对比计算,最后利用特高压交流电晕笼进行了相关对比试验。计算与试验结果均表明:新增2根子导线优化排列位置位于分裂圆底部比较合理;无线电干扰和可听噪声方面,(8+2)×400 mm^2导线在相同方式下比常规8×630 mm^2导线小2 d B左右,与10×400 mm^2导线相差不大。且(8+2)×400 mm^2导线总截面更小,电磁环境更优,有一定工程推广价值。

以双差定位方法对郯城8(1/2)级地震震中附近现代小震重新定位

用双差定位方法对1668年郯城8(1/2)级地震震中附近1974年以来400余个中、小地震重新进行定位。结果显示现代中、小地震震中分布仍集中于地震破裂带上,带状集中区与地质考察推测得到的发震断层安丘-莒县断裂的走向吻合。

基于MgAl-8-HQ LDH复合粒子选择性检测Fe^(3+)的荧光传感器

为实现对Fe^(3+)高选择和简便检测,基于镁铝层状双氢氧化物(MgAl LDH)层板的Al^(3+)的可调控性及易与8-羟基喹啉(8-HQ)发生配位作用,设计制备了8-HQ插层配位的高荧光性8-羟基喹啉镁铝层状双氢氧化物(Mg_xAl-8-HQ LDH),同时采用IR,XRD,UV-Vis及分子荧光光谱仪表征其结构和性能。IR分析表明8-HQ与铝离子生成C-O-Al和C-N-Al配位键;XRD揭示8-HQ插入MgAl LDH的层板间,致(003)衍射峰向2θ低角度方向移动,衍射峰强度随Mg与Al摩尔比增加而增强;因MgAl LDH层间的8-HQ与Al^(3+)配位,致8-HQ在314 nm处的吸收峰消失,同时在376 nm处出现了金属离子与配体之间的跃迁吸收峰;荧光分析表明:Mg_xAl-8-HQ LDH的荧光强度随Al^(3+)含量降低而增强,当镁铝离子物质的量之比为4∶1时,荧光强度显著强于8-羟基喹啉铝。通过研究金属离子对Mg_4Al-8-HQ LDH粒子荧光光谱的影响,发现该粒子对金属离子表现出显著的选择和差异性,尤其对Fe^(3+)具有高选择性。进一步研究[Fe^(3+)]对Mg_4Al-8-HQ LDH粒子的溶液颜色及荧光强度的影响表明:在10^(-6)~10^(-2)mol·L^(-1)内随[Fe^(3+)]增加,Mg_4Al-8-HQ LDH粒子溶液的颜色由浅黄色变成墨绿色,故可实现上述浓度范围内Fe^(3+)的比色传感;同时其荧光强度显著降低,当[Fe^(3+)]为10^(-3)mol·L^(-1)可完全猝灭其荧光,当-log[Fe^(3+)]为3~6时,-log[Fe^(3+)]与溶液荧光强度呈负相关函数关系,可实现对Fe^(3+)的高选择和灵敏性荧光传感检测,据此本实验成功建立了Mg_A Al-8-HQ LDH粒子荧光和比色双重传感检测Fe^(3+)的新方法。

8寸插秧机在双季稻区的应用及配套农艺技术研究

为了研究不同行距插秧机对双季稻产量的影响及配套农艺技术,以11个早晚稻品种为材料,设置8寸和9寸2个机插行距,系统测定不同处理的机插质量和产量。结果表明:8寸插秧机的机插质量高,作业性能稳定;8寸插秧机较9寸插秧机,早稻增产347.30 kg/hm2,增产率达5.49%,晚稻增产579.54 kg/hm2,增产率达9.12%。基于试验支持研究和已有相关研究成果,制定了适应8寸插秧机的配套农艺技术。

定边罗庞塬地区长8油层组双物源对石油分布的控制

定边罗庞塬地区长8油层组是定边油田主力产油层,储层及石油分布非均质性很强,长8油层组沉积微相及石油分布明显受西北及东北方向双物源控制,因此研究长8油层组双物源的交汇位置、双物源岩石学特征的差异性及双物源对沉积微相和石油分布的控制作用具有重要实践价值。通过铸体薄片、X-衍射全岩分析技术研究了双物源岩石学特征,通过沉积相分析技术研究了长8油层组的相带展布规律,通过油藏分析技术研究了长8油层组石油分布规律。结果表明:研究区长8油层组西北物源具有高石英、低长石及高沉积岩岩屑、高变质岩岩屑、低岩浆岩岩屑的特征,东北物源具有高长石、低石英及高岩浆岩岩屑、高变质岩岩屑、低沉积岩岩屑的特征;长8油层组在研究区为三角洲前缘沉积,西部相带呈北西向延伸,在研究区东部相带呈北东向延伸,在研究区中部叠合连片;长8油层组的石油分布且具有明显的分割性,分别呈北西向、北东向延伸。上述研究反映出定边罗庞塬地区长8油层组石油的宏观分布明显受北西、北东向双物源的控制。

特发性血小板减少性紫癜患儿外周血T_（48）^（＋＋）双标记细胞的分布

用减数法观察了３０例原发性血小板减少性紫癜（ＩＴＰ）患儿外周血Ｔ双标记细胞（ＤＬＣ）的分布及ＤＬＣ分别与血小板计数（ＰＣ）、血小板表面相关抗体ＩｇＧ（ＰＡＩｇＧ）、ＨＬＡ－ＤＲ＋Ｔ细胞及ＨＬＡ─ＤＱ＋Ｔ细胞的关系。发现：ＩＴＰ患儿的ＤＬＣ较正常对照组显著升高（Ｐ＜０．０１）。ＤＬＣ与ＰＡＩｇＧ、ＨＬＡ─ＤＲ＋Ｔ及ＨＬＡ─ＤＱ＋Ｔ均呈显著正相关（Ｐ分别＜０．０５、０．０５及０．０１），与ＰＣ呈负相关（Ｐ＜０．０５）。提示ＤＬＣ在外周血中的大量出现与ＩＴＰ发病密切相关。

双8轨迹自动行进越障小车试验研究

越障小车是以重力势能为驱动,运行双8轨迹,连续绕过障碍桩的三轮小车。根据小车的基本结构,针对各组成模块进行设计,提出槽轮机构与曲柄滑块作为转向控制机构的双8轨迹越障小车。调节导轨的长度及导轨离转向前轮的距离,实现前轮偏角及4次转向时间间隔调节,得到不同小车双8字轨迹的形状,确定轨迹行驶的最优参数。最后,制作小车实物模型,验证了调试结果的可行性,为小车研究奠定了理论依据。