Although oligonucleotide-directed mutagenesis has been widely applied in the field of molecular biology, this method has some disadvantages: It is time-consuming and overelaborate, besides only a single base mutation ...Although oligonucleotide-directed mutagenesis has been widely applied in the field of molecular biology, this method has some disadvantages: It is time-consuming and overelaborate, besides only a single base mutation of the gene can be obtained by a single operation.展开更多
文摘Although oligonucleotide-directed mutagenesis has been widely applied in the field of molecular biology, this method has some disadvantages: It is time-consuming and overelaborate, besides only a single base mutation of the gene can be obtained by a single operation.
