Age, maturation and population structure of the Humboldt squid Dosidicus gigas were studied based on random sampling of the Chinese jigging fishery off the Peruvian Exclusive Economic Zones （EEZ） during 2008-2010. E...Age, maturation and population structure of the Humboldt squid Dosidicus gigas were studied based on random sampling of the Chinese jigging fishery off the Peruvian Exclusive Economic Zones （EEZ） during 2008-2010. Estimated ages ranged from 144 to 633 days, confirming that the squid is a short-lived species with longevity no longer than 2 years. Occurrence of mature females and hatching in each month indicated that Humboldt squid spawned year-round. Back-calculated hatching dates for the samples were from January 22^nd, 2008 to April 22nd, 2010 with a peak between January and March. Two size-based and two hatching date-based populations could be defined from mantle length （ML） at maturity and back-calculated hatching dates, respectively. Females matured at a larger size than males, and there was a significant difference in ML at maturity between the two hatching groups （P〈0.05）. The waters adjacent to 1 l^S off the Peruvian EEZ may be a potential spawning ground. This study shows the complexity of the population structure and large variability in key life history parameters in the Humboldt squid off the Peruvian EEZ, which should be considered in the assessment and management of this important resource.展开更多
The spatial scale(?shing grid) of ?sheries research af fects the observed spatial patterns of?sheries resources such as catch-per-unit-ef fort(CPUE) and ?shing ef fort. We examined the scale impact of high value(HH) c...The spatial scale(?shing grid) of ?sheries research af fects the observed spatial patterns of?sheries resources such as catch-per-unit-ef fort(CPUE) and ?shing ef fort. We examined the scale impact of high value(HH) clusters of the annual ?shing ef fort for Dosidicus gigas of fshore Peru from 2009 to 2012.For a multi-scale analysis, the original commercial ?shery data were tessellated to twelve spatial scales from 6′ to 72′ with an interval of 6′. Under these spatial scales, D. gigas clusters were identi?ed using the Anselin Local Moran's I. Statistics including the number of points, mean CPUE, standard deviation(SD),skewness, kurtosis, area and centroid were calculated for these HH clusters. We found that the z-score of global Moran's I and the number of points for HH clusters follow a power law scaling relationship from2009 to 2012. The mean ef fort and its SD also follow a power law scaling relationship from 2009 to 2012.The skewness follows a linear scaling relationship in 2010 and 2011 but ?uctuates with spatial scale in2009 and 2012; kurtosis follows a logarithmic scale relationship in 2009, 2011 and 2012 but a linear scale relationship in 2010. Cluster area follows a power law scaling relationship in 2010 and 2012, a linear scaling relationship in 2009, and a quadratic scaling relationship in 2011. Based on the peaks of Moran's I indices and the multi-scale analysis, we conclude that the optimum scales are 12′ in 2009 ? 2011 and 6′ in 2012, while the coarsest allowable scales are 48′ in 2009, 2010 and 2012, and 60′ in 2011. Our research provides the best spatial scales for conducting spatial analysis of this pelagic species, and provides a better understanding of scaling behavior for the ?shing ef fort of D. gigas in the of fshore Peruvian waters.展开更多
The jumbo flying squid(Dosidicus gigas) population was surveyed with the help of Chinese squid jigging vessels off the Costa Rica Dome(4°–11°N, 90°–100°W) in 2009 and 2010. The daily catch of D. ...The jumbo flying squid(Dosidicus gigas) population was surveyed with the help of Chinese squid jigging vessels off the Costa Rica Dome(4°–11°N, 90°–100°W) in 2009 and 2010. The daily catch of D. gigas in the two survey cruises ranged from 0 to 5.5 t and was mostly obtained from the areas bounded by 6°–9°N and 91°–94°W and by 6°30′–7°30′N and 96°–97°W. The sea surface temperature in the areas yielding the most catch ranged from 27.5 to 29℃. The sex ratio of the total catch was 3.75:1(female: male). The mantle length of the squid ranged from 211 to 355 mm(male) and from 204 to 429 mm(female) with an average of 297.9 and 306.7 mm, respectively. In the relationship of the mantle length(mm) and body weight(g) of the squid, there was no significant difference between sexes. The female and male were at a similar maturity, and most individuals are maturing or have matured with a few females being spent. The size(mantle length) and age at the first sexual maturity were 297 mm and 195 d in females, and less than 211 mm and 130 d in males, respectively. Most of the sampled stomachs(70.6%) had no food remains. The major preys of the squids were fish, cephalopods and crustaceans, with the most abundant Myctophum orientale and D. gigas. The preys in more than 65% of the non-empty sampled stomachs evidenced the cannibalism of D. gigas. The results improved current understanding of the fishery biology of D. gigas off the Costa Rica Dome, which may facilitate the assessment and management of relative fishery resources.展开更多
We examined spatially clustered distribution of jumbo flying squid(Dosidicus gigas) in the offshore waters of Peru bounded by 78?–86?W and 8?–20?S under 0.5?×0.5? fishing grid. The study is based on the catch-p...We examined spatially clustered distribution of jumbo flying squid(Dosidicus gigas) in the offshore waters of Peru bounded by 78?–86?W and 8?–20?S under 0.5?×0.5? fishing grid. The study is based on the catch-per-unit-effort(CPUE) and fishing effort from Chinese mainland squid jigging fleet in 2003–2004 and 2006–2013. The data for all years as well as the eight years(excluding El Ni?o events) were studied to examine the effect of climate variation on the spatial distribution of D. gigas. Five spatial clusters reflecting the spatial distribution were computed using K-means and Getis-Ord Gi* for a detailed comparative study. Our results showed that clusters identified by the two methods were quite different in terms of their spatial patterns, and K-means was not as accurate as Getis-Ord Gi*, as inferred from the agreement degree and receiver operating characteristic. There were more areas of hot and cold spots in years without the impact of El Ni?o, suggesting that such large-scale climate variations could reduce the clustering level of D. gigas. The catches also showed that warm El Ni?o conditions and high water temperature were less favorable for D. gigas offshore Peru. The results suggested that the use of K-means is preferable if the aim is to discover the spatial distribution of each sub-region(cluster) of the study area, while Getis-Ord Gi* is preferable if the aim is to identify statistically significant hot spots that may indicate the central fishing ground.展开更多
The spatiotemporal distribution and relationship between nominal catch-per-unit-ef fort(CPUE) and environment for the jumbo flying squid( Dosidicus gigas) were examined in of fshore Peruvian waters during 2009–2013. ...The spatiotemporal distribution and relationship between nominal catch-per-unit-ef fort(CPUE) and environment for the jumbo flying squid( Dosidicus gigas) were examined in of fshore Peruvian waters during 2009–2013. Three typical oceanographic factors aff ecting the squid habitat were investigated in this research, including sea surface temperature(SST), sea surface salinity(SSS) and sea surface height(SSH). We studied the CPUE-environment relationships for D. gigas using a spatially-lagged version of spatial autoregressive(SAR) model and a generalized additive model(GAM), with the latter for auxiliary and comparative purposes. The annual fishery centroids were distributed broadly in an area bounded by 79.5°–82.7°W and 11.9°–17.1°S, while the monthly fishery centroids were spatially close and lay in a smaller area bounded by 81.0°–81.2°W and 14.3°–15.4°S. Our results show that the preferred environmental ranges for D. gigas offshore Peru were 20.9°–21.9°C for SST, 35.16–35.32 for SSS and 27.2–31.5 cm for SSH in the areas bounded by 78°–80°W/82–84°W and 15°–18°S. Monthly spatial distributions during October to December were predicted using the calibrated GAM and SAR models and general similarities were found between the observed and predicted patterns for the nominal CPUE of D. gigas. The overall accuracies for the hotspots generated by the SAR model were much higher than those produced by the GAM model for all three months. Our results contribute to a better understanding of the spatiotemporal distributions of D. gigas off shore Peru, and off er a new SAR modeling method for advancing fishery science.展开更多
An analysis was performed in this study to investigate synchronous fluctuations in abundance and distribution of Om-mastrephes bartramii in the Northwest Pacific Ocean and Dosidicus gigas in the Southeast Pacific Ocea...An analysis was performed in this study to investigate synchronous fluctuations in abundance and distribution of Om-mastrephes bartramii in the Northwest Pacific Ocean and Dosidicus gigas in the Southeast Pacific Ocean.The impacts of two Niño indices and regional water surface temperature on the two squids during 2006-2015 were evaluated,which possibly can explain the observed synchronicity.Catch per unit effort(CPUE)and the latitudinal gravity centers(LATG)of fishing effort were used to indi-cate squid abundance and distribution,respectively.The results indicated that both the CPUE and LATG showed highly interannual variations and synchronous fluctuation with significant negative associations between the two squid species from September to No-vember.Strong positive cross-correlations with 2-month lag was found between sea surface temperature(SST)anomaly in the Niño 3.4 and Niño 1+2 regions,which have significant linkage with the SST on the fishing ground of O.bartramii and D.gigas,respec-tively.Moreover,the proportion of favorable-SST area(PFSST)and the latitudinal location of the optimal SST for O.bartramii and D.gigas were positively correlated with the CPUE and LATG,respectively.Increased O.bartramii PFSST clearly corresponded to decreased D.gigas PFSST in phase as well as the latitudinal location of the optimal SST from September to November over 2006-2015.Our findings suggest that synchronous changes in abundance and distribution of the two squids were due to simultaneous variations in the PFSST and the latitudinal location of the optimal SST front which were affected by the SSTA changes in the Niño 3.4 and Niño 1+2 regions.展开更多
Abundance and distribution of jumbo flying squid(Dosidicus gigas)are evidently influenced by the changes of marine environment.In this study,the maximum entropy(MaxEnt)model was applied to examine the impacts of marin...Abundance and distribution of jumbo flying squid(Dosidicus gigas)are evidently influenced by the changes of marine environment.In this study,the maximum entropy(MaxEnt)model was applied to examine the impacts of marine environmental variables on its potential distribution,and identified habitat hotspots of D.gigas in summer and autumn along the coast of Chile.The MaxEnt model was constructed by using the fisheries data of D.gigas from summer to autumn during 2011–2017 combined with critical environmental factors including mixed layer depth(MLD),sea surface salinity(SSS),sea surface height(SSH)and water temperature at depths of 0 m,25 m,50 m,100 m,150 m,200 m,300 m,400 m and 500 m.Results showed that the actual fishing efforts of D.gigas in summer and autumn were mostly distributed in the suitable habitat,indicating that the MaxEnt model can well predict the habitat hotspots of D.gigas off Chile.The key environmental factors and their suitable ranges for D.gigas showed significant intermonthly changes from December to May.The critical environmental factors of D.gigas off Chile were MLD,SSH,water temperature at different depths in summer(Temp_25 m in December,Temp_300 m in January and Temp_400 m in February)and SSH,SSS,Temp_400 m in autumn.Our findings suggest that selecting the key environmental factors is vital to study the potential distribution of D.gigas off Chile in each month to explore its habitat hotspots.展开更多
Gladius is an accepted hard tissue for determining the age,size-specific growth,and trophic dynamics of pelagic squid.However,little is known about the inter-and intra-stock variability of gladius growth pattern and i...Gladius is an accepted hard tissue for determining the age,size-specific growth,and trophic dynamics of pelagic squid.However,little is known about the inter-and intra-stock variability of gladius growth pattern and increment deposition.In this study,the gladius growth patterns with somatic growth and gonad development were evaluated for two geographic stocks of the jumbo squid(Dosidicus gigas)in the tropical Pacific Ocean.The microstructure and periodicity of the gladius growth increments were also investigated.Results showed varied correlations between four gladius morphometric characteristics with dorsal mantle length,while growth in body weight almost followed a power model.Sexual gladius growth patterns occurred with gonad development,possibly due to different biological functions of these gladius parts and sex-specific energetic allocations.Gladius increments were observed formation in the stem and lateral plate and could be consistently enumerated.The daily periodicity of increment deposition,at least over 144 days old,was validated by comparing gladius increments with the ages obtained from the statoliths.The different oceanographic conditions between two areas likely influence the gladius growth and increment deposition and promote higher increment counts in squid from equatorial waters.Above all,these results provided new information on gladius microstructures and growth increment in different stocks of D.gigas and confirmed the use of this tissue for size-specific growth and age determination.展开更多
基金Supported by the National Natural Science Foundation of China (No.41276156)the National High Technology Research and Development Program of China (863 Program) (No. 2012AA092303)+3 种基金the Innovation Program of Shanghai Municipal Education Commission (No. 13YZ091)Shanghai Leading Academic Disciplin Projectsupported by National Distant-Water Fisheries Engineering Research Center, and Scientific Observing and Experimental Station of Oceanic Fishery Resources, Ministry of AgricultureYong Chen’s involvement in the project was supported by the Shanghai Dongfang Scholar Program
文摘Age, maturation and population structure of the Humboldt squid Dosidicus gigas were studied based on random sampling of the Chinese jigging fishery off the Peruvian Exclusive Economic Zones （EEZ） during 2008-2010. Estimated ages ranged from 144 to 633 days, confirming that the squid is a short-lived species with longevity no longer than 2 years. Occurrence of mature females and hatching in each month indicated that Humboldt squid spawned year-round. Back-calculated hatching dates for the samples were from January 22^nd, 2008 to April 22nd, 2010 with a peak between January and March. Two size-based and two hatching date-based populations could be defined from mantle length （ML） at maturity and back-calculated hatching dates, respectively. Females matured at a larger size than males, and there was a significant difference in ML at maturity between the two hatching groups （P〈0.05）. The waters adjacent to 1 l^S off the Peruvian EEZ may be a potential spawning ground. This study shows the complexity of the population structure and large variability in key life history parameters in the Humboldt squid off the Peruvian EEZ, which should be considered in the assessment and management of this important resource.
基金Supported by the National Natural Science Foundation of China(No.41406146)the Laboratory for Marine Fisheries Science and Food Production Processes at Qingdao National Laboratory for Marine Science and Technology of China(No.2017-1A02)the Shanghai Universities First-class Disciplines Project-Fisheries(A)
文摘The spatial scale(?shing grid) of ?sheries research af fects the observed spatial patterns of?sheries resources such as catch-per-unit-ef fort(CPUE) and ?shing ef fort. We examined the scale impact of high value(HH) clusters of the annual ?shing ef fort for Dosidicus gigas of fshore Peru from 2009 to 2012.For a multi-scale analysis, the original commercial ?shery data were tessellated to twelve spatial scales from 6′ to 72′ with an interval of 6′. Under these spatial scales, D. gigas clusters were identi?ed using the Anselin Local Moran's I. Statistics including the number of points, mean CPUE, standard deviation(SD),skewness, kurtosis, area and centroid were calculated for these HH clusters. We found that the z-score of global Moran's I and the number of points for HH clusters follow a power law scaling relationship from2009 to 2012. The mean ef fort and its SD also follow a power law scaling relationship from 2009 to 2012.The skewness follows a linear scaling relationship in 2010 and 2011 but ?uctuates with spatial scale in2009 and 2012; kurtosis follows a logarithmic scale relationship in 2009, 2011 and 2012 but a linear scale relationship in 2010. Cluster area follows a power law scaling relationship in 2010 and 2012, a linear scaling relationship in 2009, and a quadratic scaling relationship in 2011. Based on the peaks of Moran's I indices and the multi-scale analysis, we conclude that the optimum scales are 12′ in 2009 ? 2011 and 6′ in 2012, while the coarsest allowable scales are 48′ in 2009, 2010 and 2012, and 60′ in 2011. Our research provides the best spatial scales for conducting spatial analysis of this pelagic species, and provides a better understanding of scaling behavior for the ?shing ef fort of D. gigas in the of fshore Peruvian waters.
基金the supports of the two scientific surveys made by Fenghui No 16 and Zhe Yunyu No 807funded by National Nature Science Foundation of China (NSFC 41276156)+5 种基金National High-tech R&D Program of China (863 Program 2012AA092303)Project of Shanghai science and technology innovation (12231203900)Industrialization program of National Development and Reform Commission (2159999)Shanghai Leading Academic Discipline Projectsupported by National Distant-water Fisheries Engineering Research Center
文摘The jumbo flying squid(Dosidicus gigas) population was surveyed with the help of Chinese squid jigging vessels off the Costa Rica Dome(4°–11°N, 90°–100°W) in 2009 and 2010. The daily catch of D. gigas in the two survey cruises ranged from 0 to 5.5 t and was mostly obtained from the areas bounded by 6°–9°N and 91°–94°W and by 6°30′–7°30′N and 96°–97°W. The sea surface temperature in the areas yielding the most catch ranged from 27.5 to 29℃. The sex ratio of the total catch was 3.75:1(female: male). The mantle length of the squid ranged from 211 to 355 mm(male) and from 204 to 429 mm(female) with an average of 297.9 and 306.7 mm, respectively. In the relationship of the mantle length(mm) and body weight(g) of the squid, there was no significant difference between sexes. The female and male were at a similar maturity, and most individuals are maturing or have matured with a few females being spent. The size(mantle length) and age at the first sexual maturity were 297 mm and 195 d in females, and less than 211 mm and 130 d in males, respectively. Most of the sampled stomachs(70.6%) had no food remains. The major preys of the squids were fish, cephalopods and crustaceans, with the most abundant Myctophum orientale and D. gigas. The preys in more than 65% of the non-empty sampled stomachs evidenced the cannibalism of D. gigas. The results improved current understanding of the fishery biology of D. gigas off the Costa Rica Dome, which may facilitate the assessment and management of relative fishery resources.
基金supported by the National Natural Science Foundation of China(41406146 and 41476129)Shanghai Universities First-class Disciplines Project Fisheries(A)
文摘We examined spatially clustered distribution of jumbo flying squid(Dosidicus gigas) in the offshore waters of Peru bounded by 78?–86?W and 8?–20?S under 0.5?×0.5? fishing grid. The study is based on the catch-per-unit-effort(CPUE) and fishing effort from Chinese mainland squid jigging fleet in 2003–2004 and 2006–2013. The data for all years as well as the eight years(excluding El Ni?o events) were studied to examine the effect of climate variation on the spatial distribution of D. gigas. Five spatial clusters reflecting the spatial distribution were computed using K-means and Getis-Ord Gi* for a detailed comparative study. Our results showed that clusters identified by the two methods were quite different in terms of their spatial patterns, and K-means was not as accurate as Getis-Ord Gi*, as inferred from the agreement degree and receiver operating characteristic. There were more areas of hot and cold spots in years without the impact of El Ni?o, suggesting that such large-scale climate variations could reduce the clustering level of D. gigas. The catches also showed that warm El Ni?o conditions and high water temperature were less favorable for D. gigas offshore Peru. The results suggested that the use of K-means is preferable if the aim is to discover the spatial distribution of each sub-region(cluster) of the study area, while Getis-Ord Gi* is preferable if the aim is to identify statistically significant hot spots that may indicate the central fishing ground.
基金Supported by the National Natural Science Foundation of China(Nos.41406146,41476129)the Natural Science Foundation of Shanghai Municipality(No.13ZR1419300)the Shanghai Universities FirstClass Disciplines Project-Fisheries(A)
文摘The spatiotemporal distribution and relationship between nominal catch-per-unit-ef fort(CPUE) and environment for the jumbo flying squid( Dosidicus gigas) were examined in of fshore Peruvian waters during 2009–2013. Three typical oceanographic factors aff ecting the squid habitat were investigated in this research, including sea surface temperature(SST), sea surface salinity(SSS) and sea surface height(SSH). We studied the CPUE-environment relationships for D. gigas using a spatially-lagged version of spatial autoregressive(SAR) model and a generalized additive model(GAM), with the latter for auxiliary and comparative purposes. The annual fishery centroids were distributed broadly in an area bounded by 79.5°–82.7°W and 11.9°–17.1°S, while the monthly fishery centroids were spatially close and lay in a smaller area bounded by 81.0°–81.2°W and 14.3°–15.4°S. Our results show that the preferred environmental ranges for D. gigas offshore Peru were 20.9°–21.9°C for SST, 35.16–35.32 for SSS and 27.2–31.5 cm for SSH in the areas bounded by 78°–80°W/82–84°W and 15°–18°S. Monthly spatial distributions during October to December were predicted using the calibrated GAM and SAR models and general similarities were found between the observed and predicted patterns for the nominal CPUE of D. gigas. The overall accuracies for the hotspots generated by the SAR model were much higher than those produced by the GAM model for all three months. Our results contribute to a better understanding of the spatiotemporal distributions of D. gigas off shore Peru, and off er a new SAR modeling method for advancing fishery science.
基金This study was financially supported by the National Key R&D Program of China(No.2019YFD0901405)the National Natural Science Foundation of China(No.41906073)+2 种基金the Natural Science Foundation of Shanghai(No.19ZR1423000)the Open Fund for Key Laboratory of Su-stainable Exploitation of Oceanic Fisheries Resources in Marine Fisheries Research Institute of Zhejiang(No.2020 KF002)the Shanghai Universities First-Class Disci-plines Project(Fisheries A).
文摘An analysis was performed in this study to investigate synchronous fluctuations in abundance and distribution of Om-mastrephes bartramii in the Northwest Pacific Ocean and Dosidicus gigas in the Southeast Pacific Ocean.The impacts of two Niño indices and regional water surface temperature on the two squids during 2006-2015 were evaluated,which possibly can explain the observed synchronicity.Catch per unit effort(CPUE)and the latitudinal gravity centers(LATG)of fishing effort were used to indi-cate squid abundance and distribution,respectively.The results indicated that both the CPUE and LATG showed highly interannual variations and synchronous fluctuation with significant negative associations between the two squid species from September to No-vember.Strong positive cross-correlations with 2-month lag was found between sea surface temperature(SST)anomaly in the Niño 3.4 and Niño 1+2 regions,which have significant linkage with the SST on the fishing ground of O.bartramii and D.gigas,respec-tively.Moreover,the proportion of favorable-SST area(PFSST)and the latitudinal location of the optimal SST for O.bartramii and D.gigas were positively correlated with the CPUE and LATG,respectively.Increased O.bartramii PFSST clearly corresponded to decreased D.gigas PFSST in phase as well as the latitudinal location of the optimal SST from September to November over 2006-2015.Our findings suggest that synchronous changes in abundance and distribution of the two squids were due to simultaneous variations in the PFSST and the latitudinal location of the optimal SST front which were affected by the SSTA changes in the Niño 3.4 and Niño 1+2 regions.
基金supported by the National Key R&D Program of China(No.2019YFD0901405)the National Natural Science Foundation of China(Nos.41906073,31900333)the Natural Science Foundation of Shanghai(No.19ZR1423000).
文摘Abundance and distribution of jumbo flying squid(Dosidicus gigas)are evidently influenced by the changes of marine environment.In this study,the maximum entropy(MaxEnt)model was applied to examine the impacts of marine environmental variables on its potential distribution,and identified habitat hotspots of D.gigas in summer and autumn along the coast of Chile.The MaxEnt model was constructed by using the fisheries data of D.gigas from summer to autumn during 2011–2017 combined with critical environmental factors including mixed layer depth(MLD),sea surface salinity(SSS),sea surface height(SSH)and water temperature at depths of 0 m,25 m,50 m,100 m,150 m,200 m,300 m,400 m and 500 m.Results showed that the actual fishing efforts of D.gigas in summer and autumn were mostly distributed in the suitable habitat,indicating that the MaxEnt model can well predict the habitat hotspots of D.gigas off Chile.The key environmental factors and their suitable ranges for D.gigas showed significant intermonthly changes from December to May.The critical environmental factors of D.gigas off Chile were MLD,SSH,water temperature at different depths in summer(Temp_25 m in December,Temp_300 m in January and Temp_400 m in February)and SSH,SSS,Temp_400 m in autumn.Our findings suggest that selecting the key environmental factors is vital to study the potential distribution of D.gigas off Chile in each month to explore its habitat hotspots.
基金This work was supported by National Natural Science Foundation of China(41541042)National Natural Science Foundation of Shanghai(17ZR1413000)Shanghai Leading Academic Discipline Project(Fisheries Discipline)and Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology(2017-1A03).
文摘Gladius is an accepted hard tissue for determining the age,size-specific growth,and trophic dynamics of pelagic squid.However,little is known about the inter-and intra-stock variability of gladius growth pattern and increment deposition.In this study,the gladius growth patterns with somatic growth and gonad development were evaluated for two geographic stocks of the jumbo squid(Dosidicus gigas)in the tropical Pacific Ocean.The microstructure and periodicity of the gladius growth increments were also investigated.Results showed varied correlations between four gladius morphometric characteristics with dorsal mantle length,while growth in body weight almost followed a power model.Sexual gladius growth patterns occurred with gonad development,possibly due to different biological functions of these gladius parts and sex-specific energetic allocations.Gladius increments were observed formation in the stem and lateral plate and could be consistently enumerated.The daily periodicity of increment deposition,at least over 144 days old,was validated by comparing gladius increments with the ages obtained from the statoliths.The different oceanographic conditions between two areas likely influence the gladius growth and increment deposition and promote higher increment counts in squid from equatorial waters.Above all,these results provided new information on gladius microstructures and growth increment in different stocks of D.gigas and confirmed the use of this tissue for size-specific growth and age determination.
