Naringin exists in a wide range of Chinese herbal medicine and has proven to possess several pharmacological properties.In this study,PC12,HepG2 cells,and female Drosophila melanogaster were used to investigate the an...Naringin exists in a wide range of Chinese herbal medicine and has proven to possess several pharmacological properties.In this study,PC12,HepG2 cells,and female Drosophila melanogaster were used to investigate the antioxidative and anti-aging effects of naringin and explore the underlying mechanisms.The results showed that naringin inhibited H_(2)O_(2)-induced decline in cell viability and decreased,the content of reactive oxygen species in cells.Meanwhile,naringin prolonged the lifespan of flies,enhanced the abilities of climbing and the resistance to stress,improved the activities of antioxidant enzymes,and decreased malondialdehyde content.Naringin also improved intestinal barrier dysfunction and reduced abnormal proliferation of intestinal stem cells.Moreover,naringin down-regulated the mRNA expressions of inr,chico,pi 3k,and akt-1,and up-regulated the mRNA expressions of dilp2,dilp3,dilp5,and foxo,thereby activating autophagy-related genes and increasing the number of lysosomes.Furthermore,the mutant stocks assays and computer molecular simulation results further indicated that naringin delayed aging by inhibiting the insulin signaling(IIS)pathway and activating the autophagy pathway,which was consistent with the result of network pharmacological predictions.展开更多
Neuroscience and neurology research is dominated by experimentation with rodents.Around 75%of neurology disease-associated genes have orthologs in Drosophila mel-anogaster,the fruit fly amenable to complex neurologica...Neuroscience and neurology research is dominated by experimentation with rodents.Around 75%of neurology disease-associated genes have orthologs in Drosophila mel-anogaster,the fruit fly amenable to complex neurological and behavioral investiga-tions.However,non-vertebrate models including Drosophila have so far been unable to significantly replace mice and rats in this field of studies.One reason for this situ-ation is the predominance of gene overexpression(and gene loss-of-function)meth-odologies used when establishing a Drosophila model of a given neurological disease,a strategy that does not recapitulate accurately enough the genetic disease condi-tions.I argue here the need for a systematic humanization approach,whereby the Drosophila orthologs of human disease genes are replaced with the human sequences.This approach will identify the list of diseases and the underlying genes that can be adequately modeled in the fruit fly.I discuss the neurological disease genes to which this systematic humanization approach should be applied and provide an example of such an application,and consider its importance for subsequent disease modeling and drug discovery in Drosophila.I argue that this paradigm will not only advance our un-derstanding of the molecular etiology of a number of neurological disorders,but will also gradually enable researchers to reduce experimentation using rodent models of multiple neurological diseases and eventually replace these models.展开更多
The purpose of this study was to explore the potential of the development and application of Paecilomyces hepiali,a fungus with edible and medicinal value,as a foodborne antioxidant and anti-aging agent.Its n-butanol ...The purpose of this study was to explore the potential of the development and application of Paecilomyces hepiali,a fungus with edible and medicinal value,as a foodborne antioxidant and anti-aging agent.Its n-butanol extract(PHE)from rice cultures was selected for anti-aging experiment because of significant free radical scavenging activity in vitro.In vivo,PHE could significantly prolong the mean lifespan,50%survival days,and the maximum lifespan of Drosophila on a high-fat diet.It is amazing that the mean lifespan increased from 19.1 days to 32.9 days,50%survival days increased from 15.7 days to 34.3 days,and the maximum lifespan extended from 44.7 days to 52.7 days,when the high-fat female Drosophila model was fed with 10μg/mL PHE.Further research showed that PHE reduced the accumulation of peroxide products and increased the activity of antioxidant enzymes.Then,through antioxidant activity tracking,dimerumic acid(compound 1,the IC_(50) value of 3.4μg/mL on DPPH free radicals scavenging activity),4,5-dihydroxy-3-methoxypentanoic acid(compound 2,new compound),and thymidine(compound 3)were isolated from PHE.It is worth mentioning that dimerumic acid,the major antioxidant compound of PHE(content up to 3%),was discovered in P.hepiali for the first time.It was concluded that PHE showed excellent anti-aging activity at a very low concentration on fat diet-induced high fat Drosophila melanogaster,and dimerumic acid may be its main material basis.These results indicated that PHE had the potential to be developed as antioxidant and anti-aging agent in the healthcare industry.展开更多
The conditional mutations in drosophila were obtained by γ-irradiation and selected using the test for dominant lethality. The conditional mutations survive under permissive genetic conditions and, additionally, comm...The conditional mutations in drosophila were obtained by γ-irradiation and selected using the test for dominant lethality. The conditional mutations survive under permissive genetic conditions and, additionally, commence to display novel properties. One of such properties is a recessive lethality. Ten conditional mutations that displayed recessive lethality were mapped with the help of a standard set of deletions. Half mutations contained two and more lethal defects. The fact that a large number of the lethal defects are associated with one mutation suggests that γ-irradiation is the most unlikely cause of the defects. One of the conditional mutations carried four lethal regions and had a Small barrel (Smba) visual phenotype. The Smba phenotype in the Smba/ In(2LR) Cy strain is inherited according to a parental type and disappears in the Smba/In(2LR) Pm strain. Lethality in two of the four lethal regions also disappears in this strain. A separate experiment was conducted to clarify how these regions lost a lethal manifestation after the In(2LR) Cy chromosome in the Smba/In(2LR) Cy strain was replaced with the In(2LR) Pm chromosome. The process of disappearance of the Smba phenotype was also observed in three Smba/In(2LR) Cy substocks. These data suggest that the regions of multiple recessive lethality emerge in a secondary manner under the effect of the earlier formed radiation-induced mutation in ontogene. It is assumed that the recessive lethal regions are the ontogenes with an altered DNA conformation. The conformation in ontogenes is changed in the germline cells during a regular “editing” of the individual development program.展开更多
Spotted wing drosophila, Drosophila suzukii (Matsumura) (SWD), is emerging as the most significant pest of berries and stone fruits worldwide. With a broad host range, thin-skinned berries are particularly susceptible...Spotted wing drosophila, Drosophila suzukii (Matsumura) (SWD), is emerging as the most significant pest of berries and stone fruits worldwide. With a broad host range, thin-skinned berries are particularly susceptible to infestation, and fruit producers are desperate for methods to control this pest. One of the keys to developing an integrated management program for SWD is to understand host preference and varietal susceptibility of highly susceptible crop hosts. Working in replicated small plots, experiments were conducted to evaluate differences in varietal susceptibility on cold hardy red primocane fruiting raspberries. Significant differences were observed in both the level of infestation and in the average number of larvae per berry by variety. In addition, it appears that there is a significant negative correlation between the level of infestation and the number of larvae per fruit with the time of sampling, but only an interaction of variety and time for the number of larvae per berry. These data provide a better understanding of the biology and ecology of this pest which is critical in refining current management practices. Knowledge of these interactions can aid in optimizing control strategies such as fine-tuning spatial and temporal control measures, which may be particularly important for early season infestations.展开更多
Spotted wing drosophila, <i>Drosophila suzukii</i> (Matsumura) (SWD), is emerging as the most significant pest of berries and stone fruits worldwide. With a broad host range, thin-skinned berries are parti...Spotted wing drosophila, <i>Drosophila suzukii</i> (Matsumura) (SWD), is emerging as the most significant pest of berries and stone fruits worldwide. With a broad host range, thin-skinned berries are particularly susceptible to infestation, and fruit producers are desperate for methods to control this pest. One of the keys to developing an integrated management program for SWD is to understand host preference and varietal susceptibility of highly susceptible crop hosts. Working in replicated small plots, experiments were conducted to evaluate differences in varietal susceptibility on cold hardy red primocane fruiting raspberries. Significant differences were observed in both the level of infestation and in the average number of larvae per berry by variety. In addition, it appears that there is a significant negative correlation between the level of infestation and the number of larvae per fruit with the time of sampling, but only an interaction of variety and time for the number of larvae per berry. These data provide a better understanding of the biology and ecology of this pest which is critical in refining current management practices. Knowledge of these interactions can aid in optimizing control strategies such as fine-tuning spatial and temporal control measures, which may be particularly important for early season infestations.展开更多
Gene pyramiding has been successfully practiced in plant breeding for developing new breeds or lines in which favorable genes from several different lines were integrated. But it has not been used in animal breeding, ...Gene pyramiding has been successfully practiced in plant breeding for developing new breeds or lines in which favorable genes from several different lines were integrated. But it has not been used in animal breeding, and some theoretical investigation and simulation analysis with respect to its strategies, feasibility and efficiency are needed before it can be implemented in animals. In this study, we used four different pure lines of Drosophila melanogaster, each of which is homozygous at a specific mutant gene with a visible effect on phenotype, to simulate the gene pyramiding process and analyze the duration and population size required in different pyramiding strategies. We finally got the ideal individuals, which are homozygous at the four target genes simultaneously. This study demonstrates that gene pyramiding is feasible in animal breeding and the interaction between genes may affect the final results.展开更多
TG-interacting factors (TGIFs) belong to a family of TALE-homeodomain proteins including TGIF, TGIF2, and TGIF2LX/Y (TGIF2 like on X or Y chromosome) in human. They potentially play important functions in various ...TG-interacting factors (TGIFs) belong to a family of TALE-homeodomain proteins including TGIF, TGIF2, and TGIF2LX/Y (TGIF2 like on X or Y chromosome) in human. They potentially play important functions in various tissues during development. Mutations in TGIF are frequently associated with malformation of forebrain and facial structures; TGIF2 proteins are over-expressed in many ovarian cancer cell lines; and TGIF2LX/Y are specifically expressed in adult testis. The molecular functions of these proteins have been investigated mostly in cultured cells. TGIF and TGIF2 have been found as transcriptional repressors that modulate TGF-beta signaling. However these findings are far from sufficient to explain their mutant phenotypes or expression patterns, and the functions of TGIF2LX/Y have never been reported. Here we use Drosophila as a model system to explore the functions of TGIF family proteins in vivo. We observed in fly tissues such as fat body, epithelia, and neuronal cells, that expressing human TGIF2 or human TGIF2LX generally inhibited cell growth in size and number. Co-expressing Drosophila Myc, Cyclin E, or human c-MycS partially rescued the growth inhibition induced by human TGIFs, whereas activated insulin pathway signaling did not. Taken together, we provide in vivo evidence for the potential functions of human TGIF2 and TGIF2LX in growth control. Additionally, we confirmed that Drosophila TGIFs are transcriptional activators by assaying their activities in spermatogenesis.展开更多
Drosophila melanogaster mutants with deficient kynurenine (KYN) formation from tryptophan (TRP) have longer life span than wild type flies. Administration of alpha-methyl-TRP and 5-methyl-TRY, the inhibitors of TRP-KY...Drosophila melanogaster mutants with deficient kynurenine (KYN) formation from tryptophan (TRP) have longer life span than wild type flies. Administration of alpha-methyl-TRP and 5-methyl-TRY, the inhibitors of TRP-KYN metabolism, prolonged life span in wild-type flies. Both inhibitors are not available for human use. Berberine, an isoquinoline alkaloid isolated from berberis aristata is known as the herb widely used in traditional Chinese and Indian medicine. Berberin is a strong inhibitor of the enzyme catalyzing TRP conversion into KYN. Considering this particular feature we investigated the effect of berberine on life-and health-span in wild-type Drosophila melanogaster. The results of our study showed that Berberine extended mean, median and maximum life span of female flies. Berberine did not affect the number of pupae of filial generation and decreased their lethality. Berberine increased locomotor activity (vertical climbing). The results of the study suggest that berberine prolongs life- and improves health-span of Drosophila melanogaster. Berberine might be a candidate drug for prevention and treatment of aging and aging-associated medical and psychiatric disorders.展开更多
The fruit fly Drosophila melanogaster has been successfully used to study numerous biological processes including immune response.Flies are naturally infected with more than twenty RNA viruses making it a valid model ...The fruit fly Drosophila melanogaster has been successfully used to study numerous biological processes including immune response.Flies are naturally infected with more than twenty RNA viruses making it a valid model organism to study host-pathogen interactions during viral infections.The Drosophila antiviral immunity includes RNA interference,activation of the JAK/STAT and other signaling cascades and other mechanisms such as autophagy and interactions with other microorganisms.Here we review Drosophila as an immunological research model as well as recent advances in the field ofDrosophila antiviral immunity.展开更多
We have observed that berberine prolonged life span and improved viability of pupae and climbing activity of imagoes of wild-type Drosophila melanogaster maintained at 23°C. As a continuation of our studies of be...We have observed that berberine prolonged life span and improved viability of pupae and climbing activity of imagoes of wild-type Drosophila melanogaster maintained at 23°C. As a continuation of our studies of berberine effect on life span, we were interested to evaluate the effect of berberine of life span in flies maintained at a higher temperature (28°C) known to accelerate aging in wild type flies. Considering that genetically or pharmacologically induced deficiency of TRP conversion into KYN prolonged life span in a Drosophila model, we compared the effects of berberine, a powerful inhibitor of kynurenine (KYN) formation from tryptophan (TRP), on life span in wild type and in Drosophila melanogaster mutants (vermilion) with deficient TRP-KYN metabolism maintained at 23°C and 28°C. High (28°C) ambient temperature decreased life span in both wild type and vermilion flies. Aging accelerating effect of high temperature was more pronounced in Oregon than in vermilion flies (-60% vs. -40% decrease of mean life span, resp). Berberine attenuated the aging-accelerating effect of high temperature. Effect of berberine was more pronounced in Oregon (+46%) than in vermilion (+22%) flies. The obtained data suggested the possible involvement of TRP-KYN metabolism in the aging-acceleration effect of the high temperature and in protective effect of berberine.展开更多
基金supported by the open project of the Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,China (GMU-2022-HJZ-06)。
文摘Naringin exists in a wide range of Chinese herbal medicine and has proven to possess several pharmacological properties.In this study,PC12,HepG2 cells,and female Drosophila melanogaster were used to investigate the antioxidative and anti-aging effects of naringin and explore the underlying mechanisms.The results showed that naringin inhibited H_(2)O_(2)-induced decline in cell viability and decreased,the content of reactive oxygen species in cells.Meanwhile,naringin prolonged the lifespan of flies,enhanced the abilities of climbing and the resistance to stress,improved the activities of antioxidant enzymes,and decreased malondialdehyde content.Naringin also improved intestinal barrier dysfunction and reduced abnormal proliferation of intestinal stem cells.Moreover,naringin down-regulated the mRNA expressions of inr,chico,pi 3k,and akt-1,and up-regulated the mRNA expressions of dilp2,dilp3,dilp5,and foxo,thereby activating autophagy-related genes and increasing the number of lysosomes.Furthermore,the mutant stocks assays and computer molecular simulation results further indicated that naringin delayed aging by inhibiting the insulin signaling(IIS)pathway and activating the autophagy pathway,which was consistent with the result of network pharmacological predictions.
基金This work was supported by Swiss National Science Foundation,grant#31003A_175658 to VLK.
文摘Neuroscience and neurology research is dominated by experimentation with rodents.Around 75%of neurology disease-associated genes have orthologs in Drosophila mel-anogaster,the fruit fly amenable to complex neurological and behavioral investiga-tions.However,non-vertebrate models including Drosophila have so far been unable to significantly replace mice and rats in this field of studies.One reason for this situ-ation is the predominance of gene overexpression(and gene loss-of-function)meth-odologies used when establishing a Drosophila model of a given neurological disease,a strategy that does not recapitulate accurately enough the genetic disease condi-tions.I argue here the need for a systematic humanization approach,whereby the Drosophila orthologs of human disease genes are replaced with the human sequences.This approach will identify the list of diseases and the underlying genes that can be adequately modeled in the fruit fly.I discuss the neurological disease genes to which this systematic humanization approach should be applied and provide an example of such an application,and consider its importance for subsequent disease modeling and drug discovery in Drosophila.I argue that this paradigm will not only advance our un-derstanding of the molecular etiology of a number of neurological disorders,but will also gradually enable researchers to reduce experimentation using rodent models of multiple neurological diseases and eventually replace these models.
基金supported by the National Natural Science Foundation of China(42006094)by Fujian Natural Science Foundation(2019J05032).
文摘The purpose of this study was to explore the potential of the development and application of Paecilomyces hepiali,a fungus with edible and medicinal value,as a foodborne antioxidant and anti-aging agent.Its n-butanol extract(PHE)from rice cultures was selected for anti-aging experiment because of significant free radical scavenging activity in vitro.In vivo,PHE could significantly prolong the mean lifespan,50%survival days,and the maximum lifespan of Drosophila on a high-fat diet.It is amazing that the mean lifespan increased from 19.1 days to 32.9 days,50%survival days increased from 15.7 days to 34.3 days,and the maximum lifespan extended from 44.7 days to 52.7 days,when the high-fat female Drosophila model was fed with 10μg/mL PHE.Further research showed that PHE reduced the accumulation of peroxide products and increased the activity of antioxidant enzymes.Then,through antioxidant activity tracking,dimerumic acid(compound 1,the IC_(50) value of 3.4μg/mL on DPPH free radicals scavenging activity),4,5-dihydroxy-3-methoxypentanoic acid(compound 2,new compound),and thymidine(compound 3)were isolated from PHE.It is worth mentioning that dimerumic acid,the major antioxidant compound of PHE(content up to 3%),was discovered in P.hepiali for the first time.It was concluded that PHE showed excellent anti-aging activity at a very low concentration on fat diet-induced high fat Drosophila melanogaster,and dimerumic acid may be its main material basis.These results indicated that PHE had the potential to be developed as antioxidant and anti-aging agent in the healthcare industry.
文摘The conditional mutations in drosophila were obtained by γ-irradiation and selected using the test for dominant lethality. The conditional mutations survive under permissive genetic conditions and, additionally, commence to display novel properties. One of such properties is a recessive lethality. Ten conditional mutations that displayed recessive lethality were mapped with the help of a standard set of deletions. Half mutations contained two and more lethal defects. The fact that a large number of the lethal defects are associated with one mutation suggests that γ-irradiation is the most unlikely cause of the defects. One of the conditional mutations carried four lethal regions and had a Small barrel (Smba) visual phenotype. The Smba phenotype in the Smba/ In(2LR) Cy strain is inherited according to a parental type and disappears in the Smba/In(2LR) Pm strain. Lethality in two of the four lethal regions also disappears in this strain. A separate experiment was conducted to clarify how these regions lost a lethal manifestation after the In(2LR) Cy chromosome in the Smba/In(2LR) Cy strain was replaced with the In(2LR) Pm chromosome. The process of disappearance of the Smba phenotype was also observed in three Smba/In(2LR) Cy substocks. These data suggest that the regions of multiple recessive lethality emerge in a secondary manner under the effect of the earlier formed radiation-induced mutation in ontogene. It is assumed that the recessive lethal regions are the ontogenes with an altered DNA conformation. The conformation in ontogenes is changed in the germline cells during a regular “editing” of the individual development program.
文摘Spotted wing drosophila, Drosophila suzukii (Matsumura) (SWD), is emerging as the most significant pest of berries and stone fruits worldwide. With a broad host range, thin-skinned berries are particularly susceptible to infestation, and fruit producers are desperate for methods to control this pest. One of the keys to developing an integrated management program for SWD is to understand host preference and varietal susceptibility of highly susceptible crop hosts. Working in replicated small plots, experiments were conducted to evaluate differences in varietal susceptibility on cold hardy red primocane fruiting raspberries. Significant differences were observed in both the level of infestation and in the average number of larvae per berry by variety. In addition, it appears that there is a significant negative correlation between the level of infestation and the number of larvae per fruit with the time of sampling, but only an interaction of variety and time for the number of larvae per berry. These data provide a better understanding of the biology and ecology of this pest which is critical in refining current management practices. Knowledge of these interactions can aid in optimizing control strategies such as fine-tuning spatial and temporal control measures, which may be particularly important for early season infestations.
文摘Spotted wing drosophila, <i>Drosophila suzukii</i> (Matsumura) (SWD), is emerging as the most significant pest of berries and stone fruits worldwide. With a broad host range, thin-skinned berries are particularly susceptible to infestation, and fruit producers are desperate for methods to control this pest. One of the keys to developing an integrated management program for SWD is to understand host preference and varietal susceptibility of highly susceptible crop hosts. Working in replicated small plots, experiments were conducted to evaluate differences in varietal susceptibility on cold hardy red primocane fruiting raspberries. Significant differences were observed in both the level of infestation and in the average number of larvae per berry by variety. In addition, it appears that there is a significant negative correlation between the level of infestation and the number of larvae per fruit with the time of sampling, but only an interaction of variety and time for the number of larvae per berry. These data provide a better understanding of the biology and ecology of this pest which is critical in refining current management practices. Knowledge of these interactions can aid in optimizing control strategies such as fine-tuning spatial and temporal control measures, which may be particularly important for early season infestations.
基金supported by the National High-Tech Research and Development Program of China (863 Project) (No.2006AA10Z1D7)
文摘Gene pyramiding has been successfully practiced in plant breeding for developing new breeds or lines in which favorable genes from several different lines were integrated. But it has not been used in animal breeding, and some theoretical investigation and simulation analysis with respect to its strategies, feasibility and efficiency are needed before it can be implemented in animals. In this study, we used four different pure lines of Drosophila melanogaster, each of which is homozygous at a specific mutant gene with a visible effect on phenotype, to simulate the gene pyramiding process and analyze the duration and population size required in different pyramiding strategies. We finally got the ideal individuals, which are homozygous at the four target genes simultaneously. This study demonstrates that gene pyramiding is feasible in animal breeding and the interaction between genes may affect the final results.
基金the National Science Foundation of China (No. 305709)the National Basic Research Program of China (No. 2007CB947503).
文摘TG-interacting factors (TGIFs) belong to a family of TALE-homeodomain proteins including TGIF, TGIF2, and TGIF2LX/Y (TGIF2 like on X or Y chromosome) in human. They potentially play important functions in various tissues during development. Mutations in TGIF are frequently associated with malformation of forebrain and facial structures; TGIF2 proteins are over-expressed in many ovarian cancer cell lines; and TGIF2LX/Y are specifically expressed in adult testis. The molecular functions of these proteins have been investigated mostly in cultured cells. TGIF and TGIF2 have been found as transcriptional repressors that modulate TGF-beta signaling. However these findings are far from sufficient to explain their mutant phenotypes or expression patterns, and the functions of TGIF2LX/Y have never been reported. Here we use Drosophila as a model system to explore the functions of TGIF family proteins in vivo. We observed in fly tissues such as fat body, epithelia, and neuronal cells, that expressing human TGIF2 or human TGIF2LX generally inhibited cell growth in size and number. Co-expressing Drosophila Myc, Cyclin E, or human c-MycS partially rescued the growth inhibition induced by human TGIFs, whereas activated insulin pathway signaling did not. Taken together, we provide in vivo evidence for the potential functions of human TGIF2 and TGIF2LX in growth control. Additionally, we confirmed that Drosophila TGIFs are transcriptional activators by assaying their activities in spermatogenesis.
文摘Drosophila melanogaster mutants with deficient kynurenine (KYN) formation from tryptophan (TRP) have longer life span than wild type flies. Administration of alpha-methyl-TRP and 5-methyl-TRY, the inhibitors of TRP-KYN metabolism, prolonged life span in wild-type flies. Both inhibitors are not available for human use. Berberine, an isoquinoline alkaloid isolated from berberis aristata is known as the herb widely used in traditional Chinese and Indian medicine. Berberin is a strong inhibitor of the enzyme catalyzing TRP conversion into KYN. Considering this particular feature we investigated the effect of berberine on life-and health-span in wild-type Drosophila melanogaster. The results of our study showed that Berberine extended mean, median and maximum life span of female flies. Berberine did not affect the number of pupae of filial generation and decreased their lethality. Berberine increased locomotor activity (vertical climbing). The results of the study suggest that berberine prolongs life- and improves health-span of Drosophila melanogaster. Berberine might be a candidate drug for prevention and treatment of aging and aging-associated medical and psychiatric disorders.
基金Supported by Grants from the Academy of Finland,the Foundation for Pediatric ResearchSigrid Juselius Foundation+1 种基金Tampere Tuberculosis Foundation and Emil Aaltonen Foundation(to Rmet M)Competitive Research Funding of the Pirkanmaa Hospital District(to Rmet M and Valanne S)
文摘The fruit fly Drosophila melanogaster has been successfully used to study numerous biological processes including immune response.Flies are naturally infected with more than twenty RNA viruses making it a valid model organism to study host-pathogen interactions during viral infections.The Drosophila antiviral immunity includes RNA interference,activation of the JAK/STAT and other signaling cascades and other mechanisms such as autophagy and interactions with other microorganisms.Here we review Drosophila as an immunological research model as well as recent advances in the field ofDrosophila antiviral immunity.
文摘We have observed that berberine prolonged life span and improved viability of pupae and climbing activity of imagoes of wild-type Drosophila melanogaster maintained at 23°C. As a continuation of our studies of berberine effect on life span, we were interested to evaluate the effect of berberine of life span in flies maintained at a higher temperature (28°C) known to accelerate aging in wild type flies. Considering that genetically or pharmacologically induced deficiency of TRP conversion into KYN prolonged life span in a Drosophila model, we compared the effects of berberine, a powerful inhibitor of kynurenine (KYN) formation from tryptophan (TRP), on life span in wild type and in Drosophila melanogaster mutants (vermilion) with deficient TRP-KYN metabolism maintained at 23°C and 28°C. High (28°C) ambient temperature decreased life span in both wild type and vermilion flies. Aging accelerating effect of high temperature was more pronounced in Oregon than in vermilion flies (-60% vs. -40% decrease of mean life span, resp). Berberine attenuated the aging-accelerating effect of high temperature. Effect of berberine was more pronounced in Oregon (+46%) than in vermilion (+22%) flies. The obtained data suggested the possible involvement of TRP-KYN metabolism in the aging-acceleration effect of the high temperature and in protective effect of berberine.