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Enhanced extracellular production of alpha-lactalbumin from Bacillus subtilis through signal peptide and promoter screening
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作者 Yuqi Zhu Pengdong Sun +6 位作者 Chunjian Li Yu Zhang Yu Wang Jingyuan Li Yanfeng Liu Jian Chen Yang Deng 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第4期2310-2316,共7页
Alpha-lactalbumin(α-LA)is a major whey protein found in breast milk and plays a crucial role in the growth and development of infants.In this study,Bacillus subtilis RIK1285 harboring AprE signal peptide(SP)was selec... Alpha-lactalbumin(α-LA)is a major whey protein found in breast milk and plays a crucial role in the growth and development of infants.In this study,Bacillus subtilis RIK1285 harboring AprE signal peptide(SP)was selected as the original strain for the production ofα-LA.It was found thatα-LA was identified in the pellet after ultrasonic disruption and centrifugation instead of in the fermentation supernatant.The original strain most likely only producedα-LA intracellular,but not extracellular.To improve the expression and secretion ofα-LA in RIK1285,a library of 173 homologous SPs from the B.subtilis 168 genome was fused with target LALBA gene in the pBE-S vector and expressed extracellularly in RIK1285.SP YjcN was determined to be the best signal peptide.Bands in supernatant were observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and purified by nickel column to calculate the highest yield signal peptide.In addition,different promoters(P_(aprE),P_(43),and P_(glv))were compared and applied.The results indicated that the strain RIK1285-pBE-P_(glv)-YjcN-LALBA had the highestα-LA yield,reaching 122.04μg/mL.This study demonstrates successful expression and secretion of humanα-LA in B.subtilis and establishes a foundation for simulating breast milk for infant formulas and developing bioengineered milk. 展开更多
关键词 Bacillus subtilis ALPHA-LACTALBUMIN Bioengineering milk Signal peptide promoter screening
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Understanding the catalytic performance and deactivation behaviour of second-promoter doped Pt/WO_(χ)/γ-Al_(2)O_(3) catalysts in the glycerol hydrogenolysis for selective and cleaner production of 1,3-propanediol
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作者 Rafik Rajjak Shaikh Sittichai Damruang +2 位作者 Rais Ahmad Khan Supareak Praserthdam Piyasan Praserthdam 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第7期486-507,共22页
The selective aqueous-phase glycerol hydrogenolysis is a promising reaction to produce commercially useful 1,3-propanediol(1,3-PDO).The Pt-WOx bifunctional catalyst can catalyse the glycerol hydrogenol-ysis but the ca... The selective aqueous-phase glycerol hydrogenolysis is a promising reaction to produce commercially useful 1,3-propanediol(1,3-PDO).The Pt-WOx bifunctional catalyst can catalyse the glycerol hydrogenol-ysis but the catalyst deactivation via sintering,metal leaching,and coking can predominantly occur in the aqueous phase reaction.In this work,the effect of reaction temperature,pressure and second promoter(Cu,Fe,Rh,Mn,Re,Ru,Ir,Sn,B,and P)on catalytic performance and deactivation behaviour of Pt/WOx/-Al2O3 was investigated.When doped with Rh,Mn,Re,Ru,Ir,B,and P,the second promoter boosts catalytic activity by promoting great dispersion of Pt on support and increasing Pt surface area.The increased Bronsted acid sites lead to selective synthesis of 1,3-PDO than 1,2-propanediol(1,2-PDO).The characterization studies of fresh and spent catalysts reveal that the main cause of catalyst deactivation is the Pt sintering,as interpreted based on XRD,CO chemisorption,and TEM analyses.The Pt sintering is affected depending on the second promoter that can either or reduce the interaction between Pt,WO_(χ)/γ and Al_(2)O_(3).As an electron acceptor of Pt in Pt/WO_(χ)/γ-Al_(2)O_(3),Re and Mn as second promoters resulted in increased Pt^(2+) on the catalytic surface,which strengthens the contact between Pt andγ-Al_(2)O_(3) and WO_(χ),resulting in a decrease in Pt sintering.The metal leaching and coking are not affected by the presence of second promoter.The catalyst modified with a second promoter possesses improved catalytic activity and 1,3-PDO production,however the stability continues to remain a challenge.The present work unrav-elled the determining parameters of catalytic activity and deactivation,thus providing a promising pro-tocol toward effective catalysts for glycerol hydrogenolysis. 展开更多
关键词 Glycerol hydrogenolysis 1 3-PROPANEDIOL Metal-support interaction Second promoter Liquid phase Catalyst deactivation
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Revisiting the mitigation of coke formation:Synergism between support&promoters'role toward robust yield in the CO_(2)reformation of methane
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作者 Zahra Taherian Vahid Shahed Gharahshiran +3 位作者 Xiaoxuan Wei Alireza Khataee Yeojoon Yoon Yasin Orooji 《Nano Materials Science》 EI CAS CSCD 2024年第5期536-547,共12页
CO_(2)reformation of methane(CRM)and CO_(2)methanation are two interconnected processes with significant implications for greenhouse gas reduction and sustainable energy production for industrial purposes.While Nibase... CO_(2)reformation of methane(CRM)and CO_(2)methanation are two interconnected processes with significant implications for greenhouse gas reduction and sustainable energy production for industrial purposes.While Nibased catalysis suffers from poor stability due to coke formation or sintering,we report a super stable remedy.The active sites of mesoporous MgO were loaded using wet impregnation.The incorporation of Ni and promoters altered the physical features of the catalysts.Sm–Ni/MgO showed the smallest crystallite size,specific surface area,and pore volume.The Sm–Ni/MgO catalyst was selected as the most suitable candidate for CRM,with 82%CH4 and H2/CO ratio of approximately 100%and also for CO_(2)methanation with the conversion of carbon dioxide(82%)and the selectivity toward methane reaches 100%at temperatures above 300ᵒC.Furthermore,the Sm–Ni/MgO catalyst was stable for 900 min of continuous reaction,without significant carbon deposition.This stability was largely due to the high oxygen mobility on the catalyst surface in the presence of Sm.Overall,we demonstrated the efficacy of using promoted Ni catalysts supported by mesoporous magnesia for the improved reformation of greenhouse gases. 展开更多
关键词 Mesoporous MgO Dry reforming CO_(2)methanation Oxygen vacancies promoter
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Molecular cloning,characterization and promoter analysis of LbgCWIN1 and its expression profiles in response to exogenous sucrose during in vitro bulblet initiation in lily
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作者 Cong Gao Shiqi Li +4 位作者 Yunchen Xu Yue Liu Yiping Xia Ziming Ren Yun Wu 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第2期545-555,共11页
Lily(Lilium spp.) is an important ornamental flower, which is mainly propagated by bulbs. Cell wall invertases(CWINs), which catalyze the irreversibly conversion of sucrose into glucose and fructose in the extracellul... Lily(Lilium spp.) is an important ornamental flower, which is mainly propagated by bulbs. Cell wall invertases(CWINs), which catalyze the irreversibly conversion of sucrose into glucose and fructose in the extracellular space, are key enzymes participating in sucrose allocation in higher plants. Previous studies have shown that CWINs play an essential role in bulblet initiation process in bulbous crops, but the underlying molecular mechanism remains unclear. Here, a CWIN gene of Lilium brownii var. giganteum(Lbg) was identified and amplified from genomic DNA. Quantitative RT-PCR assays revealed that the expression level of LbgCWIN1 was highly upregulated exactly when the endogenous starch degraded in non-sucrose medium during in vitro bulblet initiation in Lbg. Phylogenetic relationship, motif, and domain analysis of LbgCWIN1 protein and CWINs in other plant species showed that all sequences of these CWIN proteins were highly conserved. The promoter sequence of LbgCWIN1 possessed a number of alpha-amylase-, phytohormone-, light-and stress-responsive cis-elements. Meanwhile, β-glucuronidase(GUS) assay showed that the 459 bp upstream fragment from the translational start site displayed maximal promoter activity. These results revealed that LbgCWIN1 might function in the process of in vitro bulblet initiation and be in the response to degradation of endogenous starch. 展开更多
关键词 Lilium brownii var.giganteum LbgCWIN1 Phylogenetic analysis promoter analysis Bulblet initiation
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Lineage-Specific Genome Architecture Links Enhancers and Non-coding Disease Variants to Target Gene Promoters
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作者 Biola M Javierre 《四川生理科学杂志》 2024年第4期860-860,共1页
Long-range interactions between regulatory elements and gene promoters play key roles in transcriptional regulation.The vast majority of interactions are uncharted,constituting a major missing link in understanding ge... Long-range interactions between regulatory elements and gene promoters play key roles in transcriptional regulation.The vast majority of interactions are uncharted,constituting a major missing link in understanding genome control.Here,we use promoter capture Hi-C to identify interacting regions of 31,253 promoters in 17 human primary hematopoietic cell types.We show that promoter interactions are highly cell type specific and enriched for links between active promoters and epigenetically marked enhancers.Promoter interactomes reflect lineage relationships of the hematopoietic tree,consistent with dynamic remodeling of nuclear architecture during differentiation.Interacting regions are enriched in genetic variants linked with altered expression of genes they contact,highlighting their functional role.We exploit this rich resource to connect non-coding disease variants to putative target promoters,prioritizing thousands of disease-candidate genes and implicating disease pathways.Our results demonstrate the power of primary cell promoter interactomes to reveal insights into genomic regulatory mechanisms underlying common diseases. 展开更多
关键词 promoter enriched INSIGHT
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Promoter and coding sequence diversity of CsCCD1 may contribute to the differential accumulation of floralβ-ionone in fresh tea leaves 被引量:3
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作者 Jingming Wang Mingyue Zhao +11 位作者 Ting Gao Yingying Feng Feiquan Wang Yuting Pan Jieyang Jin Tingting Jing Mengqian Lu Mengting Zhang Danyang Guo Xiaochun Wan Wilfried Schwab Chuankui Song 《Horticultural Plant Journal》 SCIE CAS CSCD 2023年第4期837-847,共11页
The carotenoid-derived volatileβ-ionone makes an important contribution to tea fragrance.Here,we qualitatively and quantitatively analysed 15 carotenoids in tea leaves of 13 cultivars by UHPLC-APCI-MS/MS.The 13 culti... The carotenoid-derived volatileβ-ionone makes an important contribution to tea fragrance.Here,we qualitatively and quantitatively analysed 15 carotenoids in tea leaves of 13 cultivars by UHPLC-APCI-MS/MS.The 13 cultivars were divided into two groups by PCA(Principal Component Analysis)clustering analysis of their carotenoid content,and OPLS-DA(Orthogonal projections to latent structures)indicated that the levels ofβ-carotene(VIP=2.89)and lutein(VIP=2.30)were responsible for much of the variation between the two groups.Interestingly,theβ-carotene toβ-ionone conversion rates in Group 1 were higher than in Group 2,while theβ-carotene content was significantly lower in Group 1 than in Group 2.Theβ-ionone content was significantly higher in Group 1.Pearson Correlation Coefficient calculation between the transcription level of candidate genes(CsCCD1 and CsCCD4)and the accumulation ofβ-ionone indicated that CsCCD1 may involve in the formation ofβ-ionone in 13 cultivars.Prokaryotic expression and in vitro enzyme activity assays showed that‘Chuanhuang 1’had an amino acid mutation in carotenoid cleavage dioxygenases 1(CsCCD1)compared with‘Shuchazao’,resulting in a significantly higherβ-ionone content in‘Chuanhuang 1’.Sequence analysis showed that‘Chuanhuang 1’and‘Huangdan’had different CsCCD1 promoter sequences,leading to significantly higher CsCCD1 expression andβ-ionone accumulation in‘Chuanhuang 1’.These results indicated that the promoter and coding sequence diversity of CsCCD1 might contribute to the differential accumulation ofβ-ionone in different tea cultivars. 展开更多
关键词 Camellia sinensis(L.)Kuntze Carotenoid cleavage dioxygenase Β-CAROTENE Β-IONONE promoter Differential accumulation
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OsAMT1.1 Expression by Nitrate-Inducible Promoter of OsNAR2.1 Increases Nitrogen Use Efficiency and Rice Yield
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作者 JIANG Hongzhen WANG Yamei +7 位作者 LAI Liuru LIU Xintong MIAO Changjian LIU Ruifang LI Xiaoyun TAN Jinfang GAO Zhenyu CHEN Jingguang 《Rice science》 SCIE CSCD 2023年第3期222-234,I0038,共14页
Nitrate(NO_(3)^(-))and ammonium(NH_(4)^(+))are two main inorganic nitrogen(N)sources during crop growth.Here,we enhanced the expression of OsAMT1.1,which encodes a NH_(4)^(+)transporter,using the NO_(3)^(-)-inducible ... Nitrate(NO_(3)^(-))and ammonium(NH_(4)^(+))are two main inorganic nitrogen(N)sources during crop growth.Here,we enhanced the expression of OsAMT1.1,which encodes a NH_(4)^(+)transporter,using the NO_(3)^(-)-inducible promoter of OsNAR2.1 and an ubiquitin promoter in transgenic rice plants.Under field condition of 120 kg/hm2 N,agronomic N use efficiency,N recovery efficiency and N transport efficiency,and grain yield of the pOsNAR2.1:OsAMT1.1 transgenic lines were increased compared with those of the wild type(WT)and the pUbi:OsAMT1.1 transgenic plants.Under 2.0 mmol/L NO_(3)^(-)+0.5 mmol/L NH_(4)^(+)and 0.5 mmol/L NO_(3)^(-)+2.0 mmol/L NH_(4)^(+)conditions of hydroponic culture,compared with the WT,both biomass and total N content were increased in the pOsNAR2.1:OsAMT1.1 transgenic lines.However,biomass was significantly reduced in pUbi:OsAMT1.1 transgenic plants under 0.5 mmol/L NO_(3)^(-)+2.0 mmol/L NH_(4)^(+)condition.The lines expressing pOsNAR2.1:OsAMT1.1 exhibited increased OsAMT1.1 expression and 15NH_(4)^(+)influx in roots under both 2.0 mmol/L NO_(3)^(-)+0.5 mmol/L NH_(4)^(+)and 0.5 mmol/L NO_(3)^(-)+2.0 mmol/L NH_(4)^(+)conditions.Our study showed that expression of OsAMT1.1 can be promoted when driven by the OsNAR2.1 promoter,especially under high-level nitrate condition,leading to enhancement of NH_(4)^(+)uptake,N use efficiency and grain yield. 展开更多
关键词 OsAMT1.1 OsNAR2.1 promoter nitrogen use efficiency YIELD RICE
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Methyltransferase 3A-mediated promoter methylation represses retinoic acid receptor responder 3 expression in basal-like breast cancer
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作者 YOULIN TUO XUBAO LIU 《BIOCELL》 SCIE 2023年第2期319-328,共10页
Retinoic acid receptor responder 3(RARRES3)has been characterized as a tumor suppressor in multiple types of cancer.This study aimed to examine the expression profile of RARRES3 across the PAM50 subtypes of breast can... Retinoic acid receptor responder 3(RARRES3)has been characterized as a tumor suppressor in multiple types of cancer.This study aimed to examine the expression profile of RARRES3 across the PAM50 subtypes of breast cancer.The DNA methylation status of RARRES3 was checked in the basal-like subtype,and the underlying mechanisms of its dysregulation were explored.RNA-sequencing(seq)and methylation data from The Cancer Genome Atlas were used for in-silico analysis.Basal-like representative SUM149 and MDA-MB-468 cell lines were used for in vitro and in vivo studies.Compared to tumor-adjacent normal tissues,only the basal-like tumor tissues had significantly downregulated RARRES3 expression.The methylation level of four CpG sites in the promoter region showed a strong negative correlation with RARRES3 expression.The gene coding for DNA methyltransferase 3A(DNMT3A)had consistent positive correlations with the methylation of the CpG sites.Chromatin Immunoprecipitation-quantitative polymerase chain-reaction and bisulfite sequencing PCR showed that DNMT3A could bind to the promoter region of RARRES3 and promote methylation of the CpG sites within the region.DNMT3A knockdown significantly restored RARRES3 expression at the mRNA and protein level in the two cell lines.CCK-8,colony formation,and flow cytometric analysis showed that RARRES3 overexpression attenuated the growth-promoting effects of DNMT3A overexpression and also weakened the DNMT3A overexpression-induced activation of ERK1/2 and PI3K/AKT signaling.In summary,this study revealed that DNMT3A enhances promoter methylation of the RARRES3 gene and suppresses its transcription in basal-like breast cancer.The DNMT3A-RARRES3 signaling pathway might be a potential target for the treatment of this tumor subtype. 展开更多
关键词 DNMT3A Basal-like breast cancer RARRES3 promoter methylation
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Theoretical study on the morphology of cobalt nanoparticles modulated by alkali metal promoters
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作者 Xiaobin Geng Hui Yang +3 位作者 Wenping Guo Xiaotong Liu Tao Yang Jinjia Liu 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2023年第10期2006-2013,共8页
Cobalt nanoparticles(NPs)catalysts are extensively used in heterogeneous catalytic reactions,and the addition of alkali metal promoters is a common method to modulate the catalytic performance because the catalyst'... Cobalt nanoparticles(NPs)catalysts are extensively used in heterogeneous catalytic reactions,and the addition of alkali metal promoters is a common method to modulate the catalytic performance because the catalyst's surface structures and morphologies are sensitive to the addition of promoters.However,the underlying modulation trend remains unclear.Herein,the adsorption of alkali metal promoters(Na and K)on the surfaces of face-centered-cubic(FCC)and hexagonal-closest packed(HCP)polymorphous cobalt was systematically investigated using density functional theory.Furthermore,the effect of alkali promoters on surface energies and nanoparticle morphologies was revealed on the basis of Wulff theory.For FCC-Co,the exposed area of the(111)facet in the nanoparticle increases with the adsorption coverage of alkali metal oxide.Meanwhile,the(311),(110),and(100)facets would disappear under the higher adsorption coverage of alkali metals.For HCPCo,the Wulff morphology is dominated by the(0001)and(1011)facets and is independent of the alkali metal adsorption coverage.This work provides insights into morphology modulation by alkali metal promoters for the rational design and synthesis of cobalt-based nanomaterials with desired facets and morphologies. 展开更多
关键词 cobalt nanoparticles alkali promoter density functional theory crystal morphology Wulff construction
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图书馆阅读推广数字化转型:内涵特征、行动框架与实现路径 被引量:4
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作者 赵发珍 刘艳 《图书馆杂志》 CSSCI 北大核心 2024年第2期49-56,共8页
加快推进图书馆阅读推广数字化转型,是应对时代挑战并赋能中国式现代化的必然选择。图书馆阅读推广数字化转型是将数字技术融入到阅读推广领域,并推动图书馆阅读推广全方位创新与变革,具有转型发展的复杂性、长期性,转型目标及过程的阶... 加快推进图书馆阅读推广数字化转型,是应对时代挑战并赋能中国式现代化的必然选择。图书馆阅读推广数字化转型是将数字技术融入到阅读推广领域,并推动图书馆阅读推广全方位创新与变革,具有转型发展的复杂性、长期性,转型目标及过程的阶段性,转型结果的价值性等特点。同时运用系统思维方法从“谁来转”“如何转”“转什么”“转去哪”4个维度,构建图书馆阅读推广数字化转型的行动框架。最后提出重视阅读推广数字化转型的顶层制度设计与系统规划;建立数字生态共同体,强化阅读推广主体间的协同共进;立足智慧图书馆发展规模和阶段,实行差异化转型模式等路径。 展开更多
关键词 阅读推广 数字化转型 转型框架 数字治理
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垂穗披碱草根际促生菌的分离鉴定及促生作用研究 被引量:1
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作者 任智慧 于乔 +5 位作者 高士孔 王家驹 牛德鹏 刘美丽 呼天明 付娟娟 《草地学报》 CAS CSCD 北大核心 2024年第6期1693-1701,共9页
优良的根际生长促生菌(Plant growth-promoting rhizobacteria, PGPR)是生产优质微生物菌肥的重要物质基础。本研究以广布于青藏高原的垂穗披碱草(Campeiostachys nutans Griseb.)为材料,采用平板划线法分离其PGPR,探究菌株的促生特性,... 优良的根际生长促生菌(Plant growth-promoting rhizobacteria, PGPR)是生产优质微生物菌肥的重要物质基础。本研究以广布于青藏高原的垂穗披碱草(Campeiostachys nutans Griseb.)为材料,采用平板划线法分离其PGPR,探究菌株的促生特性,通过16S测序确定菌株的分类地位,并进行盆栽试验验证PGPR的促生效果。结果表明,从垂穗披碱草根际土筛选出14株促生特性优良的PGPR,其中,有11株兼具产IAA,ACC脱氨酶以及铁载体的能力。经16S rDNA鉴定,以假单胞菌属为优势菌属,6株属于假单胞菌属(Pseudomonas),2株属于芽孢杆菌属(Bacillus),2株属于葡萄球菌属(Staphylococcus),其他四株分别属于类节杆菌属(Paenarthrobacter)、微杆菌属(Microbacterium)、不动杆菌属(Acinetobacter)和考克氏菌属(Kocuria)。盆栽试验表明,接种14株菌株对多年生黑麦草具有良好的促生效果,并能够提高其光合效率。研究结果丰富了植物根际促生菌菌种资源库,为实现农业“绿色增产”提供优质菌肥种质资源。 展开更多
关键词 青藏高原 垂穗披碱草 根际促生菌 分离筛选 促生作用
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美育视阈下的蜀绣AIGC创新设计与数字化推广研究 被引量:5
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作者 李加 张景 《包装工程》 CAS 北大核心 2024年第4期485-490,共6页
目的世代相传的蜀绣凝聚了悠久的历史价值、丰富的文化价值与极高的艺术价值。基于“美育”的视角结合AIGC技术进行创新设计有助于蜀绣的传承与发展。以人工智能的设计融合蜀绣的文化特色来开发文创产品及周边,使美育融入现代年轻人的... 目的世代相传的蜀绣凝聚了悠久的历史价值、丰富的文化价值与极高的艺术价值。基于“美育”的视角结合AIGC技术进行创新设计有助于蜀绣的传承与发展。以人工智能的设计融合蜀绣的文化特色来开发文创产品及周边,使美育融入现代年轻人的时尚生活,成为触手可及的生活美学。这既为传统文化的创新发展注入新的生机,也在传承创新中保护了非遗蜀绣的原真性。方法通过文献研究法与图像分析研究梳理蜀绣的历史与现状,并对蜀绣的传承进行实地调研,掌握目前蜀绣的纹样技艺、创新传播等情况并进行系统梳理。使用AIGC进行创新设计,并将创新设计应用于文创产品研发,最后进行数字化推广。结果通过AIGC为蜀绣带来了形式与内容上的创新,再灵活运用以线带面、绣画结合的方式,使人工智能美学助力非遗传承。结论AIGC融合蜀绣的创新设计提升了文创产品的科技精神与内涵,赋予了蜀绣传承与发展重要的时代革新意义,数字化推广也使蜀绣的传播方式与受众群体得到了有效的拓展。 展开更多
关键词 人工智能AIGC 蜀绣技艺 数字化推广 创新设计
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数字化赋能学校高质量发展——基于北京市中小学教师的调查 被引量:2
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作者 艾巧珍 徐赟 《开放教育研究》 CSSCI 北大核心 2024年第2期100-108,共9页
数字化转型已成为学校高质量发展的新动能,然而学界对数字化赋能学校发展的实践进展及存在问题尚缺乏深入研究。为了解当前中小学校数字化赋能的总体水平、主要特征和关键问题,本研究通过文献研究、政策分析、专家咨询和实践调研,从学... 数字化转型已成为学校高质量发展的新动能,然而学界对数字化赋能学校发展的实践进展及存在问题尚缺乏深入研究。为了解当前中小学校数字化赋能的总体水平、主要特征和关键问题,本研究通过文献研究、政策分析、专家咨询和实践调研,从学校管理、教学应用、环境建设、教育评价、教师发展、协同育人六个维度构建数字化赋能学校发展的指标体系并编制量表,对北京市5400余名教师开展调查。研究发现,数字化赋能中小学校发展总体程度较高,但不同维度的赋能程度存在显著差异;不同区域和不同主体的数字化实践存在显著差异。为此,数字化赋能学校发展实践应激发各主体的数字化应用意识、采取差异化区域推进策略、推进新老教师群体分类培训等,以更好地赋能基础教育学校高质量发展。 展开更多
关键词 数字化 赋能 基础教育学校 实践表征 提升路径
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高选择性部分氧化催化剂Au-Pd/TiO_(2)-SiO_(2)的设计合成与苯甲醇部分氧化反应 被引量:1
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作者 吴萍萍 舒洁 +3 位作者 王悦 张永辉 赵联明 白鹏 《中国石油大学学报(自然科学版)》 EI CAS CSCD 北大核心 2024年第1期189-196,共8页
贵金属纳米催化剂在苯甲醇部分氧化制备苯甲醛过程中具有较高的反应活性,产物苯甲醛选择性较低。采用一锅法制备TiO_(2)改性的Au-Pd/TiO_(2)-SiO_(2)纳米合金催化剂并用于苯甲醇部分氧化反应。对催化剂结构表征关联反应进行评价。结果表... 贵金属纳米催化剂在苯甲醇部分氧化制备苯甲醛过程中具有较高的反应活性,产物苯甲醛选择性较低。采用一锅法制备TiO_(2)改性的Au-Pd/TiO_(2)-SiO_(2)纳米合金催化剂并用于苯甲醇部分氧化反应。对催化剂结构表征关联反应进行评价。结果表明:少量TiO_(2)的引入会改善贵金属纳米颗粒在载体表面的分散度,调变了金钯合金表面的电子结构,提高反应活性,并显著提高载体表面Lewis酸位的数量,从而大幅提高苯甲醛选择性;大量TiO_(2)的引入会导致金钯合金程度降低,产生大量的Bronsted酸位,使得反应活性和产物选择性都大幅降低。 展开更多
关键词 贵金属纳米催化剂 部分氧化 表面酸性 助剂效应
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发展缓和医疗理念和实践后终末期患者死亡状况变化 被引量:1
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作者 戴晓艳 闫静 +1 位作者 王友培 宁晓红 《协和医学杂志》 CSCD 北大核心 2024年第1期84-88,共5页
目的 北京协和医院国际医疗部于2016年开始逐步推行缓和医疗理念和实践,本研究旨在对该理念推广前后终末期患者死亡状况进行分析,以评估缓和医疗对终末期患者死亡质量的影响。方法 回顾性收集北京协和医院国际医疗部2013年和2019年死亡... 目的 北京协和医院国际医疗部于2016年开始逐步推行缓和医疗理念和实践,本研究旨在对该理念推广前后终末期患者死亡状况进行分析,以评估缓和医疗对终末期患者死亡质量的影响。方法 回顾性收集北京协和医院国际医疗部2013年和2019年死亡的终末期患者的临床资料,对其临终前诊疗细节进行比较。结果 共入选符合纳入与排除标准的2013年终末期患者36例、2019年终末期患者37例。2013年患者中,男性19例、女性17例,平均年龄(72.1±14.0)岁,晚期肿瘤19例。2019年患者中,男性19例、女性18例,平均年龄(70.8±15.3)岁,晚期肿瘤27例。相较于2013年患者,2019年患者临终前转入ICU(0比22.2%,P=0.008)、接受心肺复苏(0比16.7%,P=0.011)、气管插管(5.4%比36.1%,P=0.001)、有创机械通气(2.7%比33.3%,P=0.001)、全肠外营养(32.4%比61.1%,P=0.014)的比例均降低,接受缓和医疗会诊(29.7%比0,P<0.001)、得到人文关怀(40.5%比16.7%,P=0.024)的比例均升高。2019年患者中,相较于未接受缓和医疗会诊患者,接受缓和医疗会诊者得到人文关怀的比例更高(90.9%比19.2%,P<0.001)。结论缓和医疗理念推广和实践后北京协和医院国际医疗部终末期患者死亡前接受有创治疗的比例明显降低,得到人文关怀的比例显著提高,死亡质量得到一定提升。 展开更多
关键词 生命终末期 缓和医疗 理念推广 会诊 死亡状况
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英国国家图书馆交互式数字叙事作品馆藏与阅读推广实践 被引量:1
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作者 于志涛 牟晓青 《数字图书馆论坛》 CSSCI 2024年第3期47-56,共10页
数字时代的图书馆只有积极适应和应用新的数字技术,方能创新服务,更好地发挥作用。以相关研究文献与采集语料为基础,对英国国家图书馆交互式数字叙事作品馆藏与阅读推广实践现状、内在理念进行梳理分析,发现英国国家图书馆以多重角色定... 数字时代的图书馆只有积极适应和应用新的数字技术,方能创新服务,更好地发挥作用。以相关研究文献与采集语料为基础,对英国国家图书馆交互式数字叙事作品馆藏与阅读推广实践现状、内在理念进行梳理分析,发现英国国家图书馆以多重角色定位积极发挥交互式数字叙事技术的催化剂作用,全面真实反映国家文化记忆现状,创新阅读推广模式,积极推进实现适应、服务和引领数字世界的目标。由此对数字时代图书馆的发展提出建议:充分认识新兴格式馆藏建设的意义,加强数字化技术研究与基础设施建设,构建阅读与推广的新形态,重视数字策展能力建设,坚守图书馆作为时代智慧宝库的价值。 展开更多
关键词 交互式数字叙事 馆藏 阅读推广 英国国家图书馆
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加快建设教育强国的语境分析与战略推进 被引量:3
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作者 秦玉友 《国家教育行政学院学报》 CSSCI 北大核心 2024年第1期11-18,共8页
新形势下,加快建设教育强国,要明确当前教育发展的战略语境,找准教育发展的现实起点,回应教育发展的主要矛盾,在战略上主动、在效果上现实地服务全面建设社会主义现代化国家的目标指向。要从多端多维加快建设教育强国,建设教育事业强国... 新形势下,加快建设教育强国,要明确当前教育发展的战略语境,找准教育发展的现实起点,回应教育发展的主要矛盾,在战略上主动、在效果上现实地服务全面建设社会主义现代化国家的目标指向。要从多端多维加快建设教育强国,建设教育事业强国、教育服务强国、教育投入强国、教育治理强国、教育学术强国、教育生态强国。要明确加快建设教育强国的推进逻辑,坚持建设教育强国“双优先”的战略定位,同步提高教育投入水平与办学效率,高度重视教育的溢出效应与办学主体的激励相容,科学布局各级教育发展主导任务的价值位序,充分释放人人成才教育与英才教育的发展红利。 展开更多
关键词 教育强国 语境分析 战略议题 推进逻辑
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开放型区域产教融合实践中心的基本特征、功能定位与推进路径 被引量:3
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作者 雷世平 谢盈盈 乐乐 《职教通讯》 2024年第2期33-40,共8页
开放型区域产教融合实践中心(简称“产教融合实践中心”)是对标产业发展前沿,由政府搭台、市场驱动,汇集产教资源,政、校、行、企等多元主体参与,旨在推动区域职业教育实训实践资源均衡发展,提升职业学校关键办学能力,为企业培养急需人... 开放型区域产教融合实践中心(简称“产教融合实践中心”)是对标产业发展前沿,由政府搭台、市场驱动,汇集产教资源,政、校、行、企等多元主体参与,旨在推动区域职业教育实训实践资源均衡发展,提升职业学校关键办学能力,为企业培养急需人才和提供技术服务的生产性实践组织形态。产教融合实践中心分为学校实践中心、公共实践中心、企业实践中心,具有参与主体的多元性、服务面向的区域性以及运营模式的多样性等基本特征;具备实践教学、社会培训、真实生产和技术服务等功能。要扎实推进产教融合实践中心建设,必须坚持政府引导,统筹谋划产教融合实践中心建设;坚持市场驱动,以股份制混合所有制推进产教融合实践中心建设;坚持同步发力,分类推进产教融合实践中心建设。 展开更多
关键词 产教融合实践中心 基本特征 功能定位 推进路径
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图书馆沉浸式阅读推广模式构建及优化策略——以长沙市图书馆为例 被引量:1
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作者 罗倩倩 龙耀华 《图书馆工作与研究》 CSSCI 北大核心 2024年第4期98-104,共7页
文章结合长沙市图书馆沉浸式阅读推广实践,基于沉浸式体验的9个要素及图书馆阅读推广的设计、实施、反馈3个阶段,构建图书馆沉浸式阅读推广模式,并提出图书馆沉浸式阅读推广优化策略,即分层分级明确目标,激发读者的思维沉浸;创新阅读推... 文章结合长沙市图书馆沉浸式阅读推广实践,基于沉浸式体验的9个要素及图书馆阅读推广的设计、实施、反馈3个阶段,构建图书馆沉浸式阅读推广模式,并提出图书馆沉浸式阅读推广优化策略,即分层分级明确目标,激发读者的思维沉浸;创新阅读推广场景,强化读者主动参与意识;增强读者心流体验,持续提高活动的吸引力。 展开更多
关键词 图书馆 沉浸式体验 心流体验 沉浸式阅读 阅读推广
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智慧国土的基本内涵与发展方向 被引量:2
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作者 陈军 翟曦 +9 位作者 王艳慧 阮于洲 刘万增 李晓波 吴洪涛 曹春华 王东华 赵鹏军 王久辉 王立刚 《时空信息学报》 2024年第2期157-167,共11页
当前,国家大力推动数字化转型和高质量发展,对自然资源与国土空间的数字化发展与应用提出了更高要求。虽然近些年国土空间数字化发展取得了很大成绩,但对标数字化转型和高质量发展的迫切要求,尚存在着与业务融合不深、技术支撑不够、整... 当前,国家大力推动数字化转型和高质量发展,对自然资源与国土空间的数字化发展与应用提出了更高要求。虽然近些年国土空间数字化发展取得了很大成绩,但对标数字化转型和高质量发展的迫切要求,尚存在着与业务融合不深、技术支撑不够、整体合力不强、发展动能不足等问题,突出表现为高质量信息供给不足、高层次分析能力不强、高层次赋能手段短缺。因此,应以智慧国土为抓手,统筹数字化发展,推进智慧化应用。本文分析了智慧国土的基本内涵,提出以数据—信息—知识为主线,统筹国土空间的数字化发展,实现一体化建模、集成化管理及知识化服务;从全面动态感知、系统精准认知及全域智慧管控的角度,提升自然资源管理和国土空间治理各项工作的智慧化应用水平,实现在实景三维空间研判、用时空信息说话、凭时空知识决策。为了切实地构建国土空间治理的数字化生态、促进国土空间治理体系和治理能力现代化,今后还要加强顶层设计,推动科技创新,开展试点示范,加快专业改革。 展开更多
关键词 数字化转型 智慧国土 基本内涵 统筹数字化发展 推进智慧化应用
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