Analysis on Therapeutic Effect of Western and Chinese Drug in Treating Intrahepatic Cholestasis of Pregnancy

Objective: To evaluate the therapeutic effect of Yinchenghao decoction (YCHD, 茵陈蒿汤) and S-adenosy-L-methionine (SAM) in treating intra-hepatic cholestasis of pregnancy (TCP) and improving prognosis of perinatal newborn babies. Methods: Sixty in-patients of TCP were randomly divided into two groups, the group A treated with YCHD and the group B treated with SAM. The symptom of itching and serum biochemical indexes, including glycocholic acid, bilirubin and transaminase, were observed after 3 weeks treat-ment, and the prognosis of perinatal newborn babies between the two groups was compared after delivery. Results: After treatment, the symptom of itching, serum levels of glycocholic acid, bilirubin and transaminase improved significantly (P< 0.05) in both groups, and the prognosis of newborn in the two groups was similar (P>0. 05). Conclusion: Both YCHD and SAM could effectively treat ICP. The former is rather cheaper, so it is more feasible for spreading.Original article on CJITWM (Chin) 2004 ;24(4): 309

<i>In-Vitro</i>Inhibitory Effect of Methanol Extracts of Chinese Herbal Drugs on Supercoiling Activity of Bacterial DNA Gyrase

A large number of Chinese herbal drugs (CHDs) exhibit antibacterial activities both in vivo and in vitro, but until now little is known regarding their inhibitory mechanisms. Bacterial DNA gyrase is a proven target for antibacterial agents. Aim of this study was to investigate the in-vitro inhibitory effect of methanol extracts of CHDs against supercoiling activity of bacterial DNA gyrase. Fifteen CHDs were selected and extracted with methanol, respectively. Inhibitory effect of the extracts on DNA gyrase was tested using gel-based DNA supercoiling assay. Among fifteen CHDs tested, methanol extracts of Lonicerae Japonicae Flos (S2), Taraxaci Herba (S7), Glycyrrhizae Radix et Rhizoma Praeparata cum Melle (S8) demonstrated an obvious inhibitory effect against supercoiling activity of DNA gyrase, and the others were either less active or could not be determined with the present method. Moreover, it was likely that S7 and S8 inhibit gyrase in a concentration-dependent manner. In conclusion, DNA supercoiling assay is a promising method to study the inhibitory activity of CHDs on bacterial DNA gyrase. Some CHDs do have gyrase-inhibitory activity as proposed. Further investigations are needed to elucidate the inhibition mechanism of these CHDs on supercoiling activity of gyrase.

Study on Effect of Chinese Herbal Medicine for Enhancing Therapeutic Efficacy on Refractory Drug Resistant Leukemia

The so-called multidrug resistance （MDR） of leukemic cells means the cross resistance of leukemic cells against multiple anti-tumor agents with different constitution and acting mechanism, which takes place simultaneous after resistance to a single contacted drug has been produced. Tumor cells with MDR would now show a low sensitivity to anti-tumor agents, making chemotherapy ineffective or of little effect. Moreover, MDR is one of the pathogenetic factors for inducing refractory leukemia.

Therapeutic Actions of the Chinese Herbal Formulae with Cold and Heat Properties and Their Effects on Ultrastructures of Synoviocytes in Rats of the Collagen-Induced Arthritis

The therapeutic actions of Qing Luo Yin (QLY清络饮) with heat property and Wen Luo Yin (WLY温络饮) with cold property on pain, swelling of the ankle, arthritis index and ultrastructures of synoviocytes were compared in rats of type II collagen-induced arthritis (CIA), with tripterygium glycosidorum (TG) used as control. The results indicated that both QLY and WLY could reduce pain, swelling of the ankle and the arthritis index of CIA, and QLY had better effects in reducing the swelling of the ankle and controlling the secondary pathological lesions as compared with WLY. Investigation on the ultrastructures of synoviocytes indicated that both QLY and WLY could reduce the number of Golgi apparatus, rough surface endoplasmic reticulum, dense bodies, matrix filaments and vacuoles so as to suppress the excessive secretion of synoviocytes in rats of CIA.

Study on Antibacterial Effect of Single Chinese Herbs on Staphylococcus aureus

[Objectives] This study was conducted to determine in-vitro antibacterial effect of 58 Chinese herbs on Staphylococcus aureus,and screen single Chinese herbs with better in-vitro antibacterial effect for formulation of prescription. [Methods]Effective components in Chinese herbs were extracted with water,and inhibition zone diameters and minimal inhibitory concentrations of the 58 Chinese herbs against S. aureus were determined by agar plate punching method and micro two-fold method,respectively. [Results] Twenty two kinds of Chinese herbs including Terminalia chebula Retz.,Anemarrhena asphodeloides Bunge.,Prunella vulgaris L,Rheum palmatum L. and Mosla chinensis Maxim. had better antibacterial effect,among which T. chebula Retz.,A. asphodeloides Bunge.,P. vulgaris L,R. palmatum L. and C. sappan Linn showed the best antibacterial effect,with average inhibition zone over 20 mm and MIC lower than 15. 6 mg/ml.[Conclusions]Traditional Chinese herbs have certain antibacterial effect,and this study will provide important theoretical reference for clinical medication and development of prescription.

In vitro study on the antiviral activity of 9 extracts of traditional Chinese herbal medicine against the human respiratory syncytial virus

Objective:To study the in vitro virucidal activity of 9 extracts of traditional Chinese herbal medicine(the water extracts of Evodia lepta,Clausena lansium,Clerodendrum cyrtophyllum,Callicarpa nudiflora,Nauclea officinalis and Elaeagnus gonyanthes,the alcohol extracts of Nauclea officinalis,Elaeagnus gonyanthes and Zanthoxylumarmatum)on human respiratory syncytial virus(HRSV).Methods:The cytotoxic effect of the extracts on cells was evaluated by a cell viability assay using the CCK-8 method,a concentration of the extracts with cell viability greater than 50%was selected for the follow-up anti-HRSV effect assay,the 50%effective concentration(EC50)was assessed by an in vitro cell infection model.Results:The EC50s of the water extract from Clerodendrum cyrtophyllum,Callicarpa nudiflora and Elaeagnus gonyanthes were 0.05 mg/mL,0.03 mg/mL and 0.05 mg/mL,and the therapeutic index(TI)of them were 18.60,21.67 and 56.80 respectively.Conclusion:The water extracts of Clerodendrum cyrtophyllum,Callicarpa nudiflora and Elaeagnus gonyanthes possess the activity of anti-HRSV virus.

A comprehensive analysis of the Bencao(herbal)small RNA Atlas reveals novel RNA therapeutics for treating human diseases

Cross-kingdom herbal mi RNA was first reported in 2012.Using a modified herbal extraction protocol,we obtained 73,677,287sequences by RNA-seq from 245 traditional Chinese Medicine(TCM),of which 20,758,257 were unique sequences.We constructed a Bencao(herbal)small RNA(s RNA)Atlas(http://bencao.bmicc.cn),annotated the sequences by sequence-based clustering,and created a nomenclature system for Bencao s RNAs.The profiles of 21,757 mi RNAs in the Atlas were highly consistent with those of plant mi RNAs in mi RBase.Using software tools,our results demonstrated that all human genes might be regulated by s RNAs from the Bencao s RNA Atlas,part of the predicted human target genes were experimentally validated,suggesting that Bencao s RNAs might be one of the main bioactive components of herbal medicines.We established roadmaps for oligonucleotide drugs development and optimization of TCM prescriptions.Moreover,the decoctosome,a lipo-nano particle consisting of 0.5%–2.5%of the decoction,demonstrated potent medical effects.We propose a Bencao(herbal)Index,including small-molecule compounds(SM),protein peptides(P),nucleic acid(N),non-nucleic and non-proteinogenic large-molecule compounds(LM)and elements from Mendeleev's periodic table(E),to quantitatively measure the medical effects of botanic medicine.The Bencao s RNA Atlas is a resource for developing gene-targeting oligonucleotide drugs and optimizing botanical medicine,and may provide potential remedies for the theory and practice of one medicine.

加味沙参麦冬汤+中药熏蒸治疗干眼症的效果及角膜荧光素染色评分分析

目的分析加味沙参麦冬汤+中药熏蒸治疗干眼症的效果及角膜荧光素染色评分。方法随机选取2021年12月—2023年12月天水市中医医院罹患干眼症的80例患者为研究对象,按随机抽签法分为对照组、观察组,每组40例;采用对照组中药熏蒸治疗,采用观察组加味沙参麦冬汤+中药熏蒸治疗,比较两组的近远视力水平、角膜荧光素染色评分、中医证候积分。结果治疗前两组近、远视力水平比较,差异无统计学意义(P均>0.05);治疗后,观察组近、远视力水平均更优,差异有统计学意义(P均<0.05)。治疗后,观察组的角膜荧光素染色评分为(0.91±0.23)分,低于对照组的(1.32±0.41)分,差异有统计学意义(t=5.515,P<0.05)。治疗前两组各项中医证候积分比较,差异无统计学意义(P>0.05);治疗后,观察组各项中医证候积分低于对照组,差异有统计学意义(P<0.05)。结论干眼症患者在临床采取加味沙参麦冬汤与中药熏蒸联合治疗方案,可明显改善症状,明显提高角膜荧光素染色评分。

Effects of Pak Ning Powder and Oral Liquid Treating Chicken Infectious Laryngotracheitis

[ Objective] To investigate the therapeutic effects of Chinese herbal medicine compound preparation ＂Pak Ning＂ on chicken infectious la- ryngotracheitis. E Methodl ＂Pak Ning＂ with different dosage forms and dosage time were used to carry out clinical observation of artificial infectious and natural infectious ILT of chickens. [ Result] The therapeutic efficacy of ＂Pak Ning＂ oral liquid water was better than powder with high cure rate, the difference was significant （P〈0.05）. compared with Ribavirin, the difference was significant （P 〈0.01 ）, and the preventive effects of feeding in advance were very well. I Conctusionl ＂Pak Ning＂ plays an important role in the treatment and prevention of ILl- with strong infectiosity and high mortality.

Network pharmacology approaches for research of Traditional Chinese Medicines

Pharmacodynamics material basis and effective mechanisms are the two main issues to decipher the mechnisms of action of Traditional Chinese medicines(TCMs)for the treatment of diseases.TCMs,in“multi-component,multi-target,multi-pathway”paradigm,show satisfactory clinical results in complex diseases.New ideas and methods are urgently needed to explain the complex interactions between TCMs and diseases.Network pharmacology(NP)provides a novel paradigm to uncover and visualize the underlying interaction networks of TCMs against multifactorial diseases.The development and application of NP has promoted the safety,efficacy,and mechanism investigations of TCMs,which then reinforces the credibility and popularity of TCMs.The current organcentricity of medicine and the“one disease-one target-one drug”dogma obstruct the understanding of complex diseases and the development of effective drugs.Therefore,more attentions should be paid to shift from“phenotype and symptom”to“endotype and cause”in understanding and redefining current diseases.In the past two decades,with the advent of advanced and intelligent technologies(such as metabolomics,proteomics,transcriptomics,single-cell omics,and artificial intelligence),NP has been improved and deeply implemented,and presented its great value and potential as the next drug-discovery paradigm.NP is developed to cure causal mechanisms instead of treating symptoms.This review briefly summarizes the recent research progress on NP application in TCMs for efficacy research,mechanism elucidation,target prediction,safety evaluation,drug repurposing,and drug design.

中药处方点评在中药饮片合理使用中的干预效果分析

目的 探讨中药处方点评在中药饮片合理使用中的干预效果。方法 选取中药处方点评实施前(2020年1~12月)中药处方2150张,实施后(2021年1~12月)中药处方2213张。比较中药处方点评实施前后不合理用药发生率及医患纠纷发生率。结果 中药处方点评实施前,用药禁忌占0.93%、大处方用药占1.30%、用法用量异常占0.79%、重复用药占0.84%、诊断与用药不符占1.67%、配伍禁忌占0.93%、处方脚注问题占0.47%,不合理用药发生率为6.93%;中药处方点评实施后,用药禁忌占0.23%、大处方用药占0.23%、用法用量异常占0.09%、重复用药占0.18%、诊断与用药不符占0.81%、配伍禁忌占0.14%、处方脚注问题占0.14%,不合理用药发生率为1.81%。中药处方点评实施后不合理用药发生率低于实施前,差异有统计学意义(P<0.05)。中药处方点评实施后医患纠纷发生率为0.09%(2/2213),低于实施前的0.47%(10/2150),差异有统计学意义(P<0.05)。结论 实施中药处方点评可规范处方管理,提高处方质量,促进合理用药。

Comparative efficacy and safety of Chinese herbal injections combined with transcatheter hepatic arterial chemoembolization in treatment of liver cancer: a bayesian network Meta-analysis

OBJECTIVE: To address the optimal Chinese herbal injections(CHIs) against liver cancer, the present network Meta-analysis is designed to investigate the comparative efficacy and safety of different CHIs.METHODS: Several electronic databases were searched up to June 1 st, 2017. The quality assessment was conducted and network Meta-analysis was performed to compare the efficacy and safety of different CHIs plus transcatheter hepatic arterial chemoembolization(TACE). Primary outcomes were 1-year and 2-year survival rate, the secondary outcomes includes the clinical effective rate, performance status and the adverse reactions(ADRs). Data analysis was applied Stata 13.0 and Win BUGS 1.4 software.RESULTS: A total of 105 randomized controlled trials(RCTs) were identified for inclusion in this analysis, with data for 7683 patients and 13 CHIs. The results suggested that Javanica oil emulsion,Huachansu injection plus TACE were more favorable for 1-year and 2-year survival rate than other CHIs. Kanglaite, Astragalus polysaccharide injection plus TACE showed superiority in the clinical effective rate and performance status over other CHIs.And Shenmai injection plus TACE was superior to reducing ADRs than other CHIs for patients with liver cancer.CONCLUSION: Our findings indicated that receiving CHIs combined with TACE may have therapeutic benefits for patients with liver cancer in improving survival rate, clinical effective rate, the performance status and alleviating the ADRs.

药物罐联合中药热熨对急性腰扭伤患者的治疗效果研究

目的探究药物罐联合中药热熨对急性腰扭伤患者的治疗效果。方法纳入2022年5月至2023年5月南通市中医院收治的130例急性腰扭伤患者,以随机数字表法分为对照组(n=65,常规药物配合物理治疗)、观察组(n=65,药物罐联合中药热熨疗法),收集患者临床疗效、疼痛评分、腰椎功能评分、疼痛发作频率与时间。结果观察组临床疗效(96.92%,63/65)明显高于对照组(84.62%,55/65),差异有统计学意义(χ^(2)=5.876,P=0.015)。治疗后,观察组疼痛数字评分法(NRS),改良日本骨科学会(M-JOA)低于对照组(P<0.05)。治疗后,观察组疼痛发作频率低于对照组,发作时间短于对照组(P<0.05)。结论与常规药物配合物理治疗措施相比,以中医为代表的中药热熨+药物罐疗法能够充分发挥中药疗效,缓解患者腰部疼痛,降低疼痛发作频率,改善患者腰椎功能与生活质量。

中医药治疗肝纤维化的优势与特色

肝纤维化是各种慢性肝病的共同病理过程,与肝脏疾病进展相关,是影响肝脏疾病转归和肝细胞癌风险的关键因素。早期过程可逆,若不及时干预治疗,程度逐渐加重终将发展为肝硬化,甚至肝细胞癌,危及人们健康。因此,积极防治肝纤维化具有重要的意义,而肝纤维化的发病机制复杂,西医治疗单一、局限,尚缺乏疗效确切且公认的抗纤维化药物,治疗存在巨大的医疗需求。随着国内外学者的努力研究,中医药治疗成为了逆转肝纤维化和抗纤维化进展的热点和重要突破点,且发现了多靶点、多途径的优势,本文将从辩证、治法、药物配伍及与现代分子机制结合等方面详细阐述中医药治疗肝纤维化的特色和优势。

板蓝根等4种中药单用和与西药联用对广泛耐药鲍曼不动杆菌的抑菌作用

目的分析板蓝根、连翘、黄芩、蒲公英单独使用和与头孢哌酮-舒巴坦(SCF)联合应用对广泛耐药(XDR)鲍曼不动杆菌(AB)的抑菌作用。方法收集2018年9月—2019年10月北京中医药大学第三附属医院30株XDR-AB非重复临床分离株。采用微量肉汤稀释法测定板蓝根、连翘、黄芩、蒲公英单用和与SCF联用对30株XDR-AB的最小抑菌浓度(MIC)。计算4种中药与SCF联用的分级抑菌浓度(FIC)指数。比较4种中药作用于XDR-AB后菌液K+和碱性磷酸酶(ALP)水平的变化。结果4种中药均有一定抑菌作用,其中连翘和黄芩MIC较低。板蓝根与SCF联用效果较好,以协同作用和相加作用为主。连翘、黄芩与SCF联用多表现为拮抗作用(FIC指数>2)。4种中药作用于XDR-AB后,菌液K+水平变化差异无统计学意义(P>0.05);连翘、黄芩作用于XDR-AB后,菌液ALP活性增强(P<0.05);板蓝根、蒲公英作用于XDR-AB前后菌液ALP活性差异无统计学意义(P>0.05)。结论连翘和黄芩单独使用的抑菌效果较好,板蓝根与SCF联用抑菌效果最好。临床可根据实际情况合理用药,以达到最佳效果。

藿香正气水的研究进展

藿香正气水是一种由十味中药组成的中药复方制剂,其组方来源于传统经典名方藿香正气散,化学成分复杂,具有抑菌、止痛、解痉等作用,临床上常用于对胃肠型感冒、腹泻、皮肤病、产后胀气等症状的治疗,有较好的疗效。现概述藿香正气水的处方组成、化学成分、药理作用和临床应用,旨在为其进一步的开发和质量标准的提升与完善提供一定的参考依据。

Effect of Chinese herbs on immunoglobulin A nephropathy:a randomized controlled trial

OBJECTIVE: The accumulation of extracellular matrix (ECM) is one of the main causes of renal fibrosis. Emerging evidence suggests that the metabolic enzyme of ECM is associated with renal fibrosis. In this study, we applied randomly controlled trial to check the curative effect of Chinese herbs on patients with immunoglobulin A nephropathy (IgAN). METHODS: Twenty-six patients were randomly divided into group A (control group) treated with Western Medicine and group B (treatment group) treated with combination of Traditional Chinese Medicine (TCM) and Western Medicine. Blood and urine tests were done before treatment and after 8-week treatment. RESULTS: The levels of the main composition of ex- tracellular matrix (MC-ECM), the metabolic enzyme of ECM (ME-ECM) and some cytokines in group B decreased more obviously than those in group A after 8-week treatment. So did the level of 24-hour urine protein. However, Metal matrix protease (MMP)-2 and vascular endothelial growth factor in group B increased more obviously than those in group A after 8-week treatment. No effects on the renal function were found in both groups. CONCLUSION: Our study provided important information on using the combination of TCM with Western Medicine to inhibit the progression of renal fibrosis in patients with IgAN.

中药联合肝动脉化疗栓塞术治疗原发性肝癌系统评价的再评价

目的:评价中药联合经肝动脉化疗栓塞术治疗原发性肝癌系统评价的方法学偏倚及其结论的可靠性。方法:计算机检索Cochrane图书馆、Pub Med、Guideline、中国生物医学文献数据库、中国期刊全文数据库与万方数据库,收集高质量中药联合经肝动脉化疗栓塞术治疗原发性肝癌的系统评价文献进行再评价。结果:共纳入12篇(项)系统评价。结果显示,中药联合肝动脉化疗栓塞治疗原发性肝癌可提高瘤体客观疗效、延长患者生存时间、提高生存质量,减少术后不良反应发生,且无严重中药相关不良反应报道。结论:中药联合经肝动脉化疗栓塞术治疗原发性肝癌的临床疗效显著优于单纯行经肝动脉化疗栓塞术,且安全性较好。

中药“717解毒合剂”治疗蝮蛇咬伤病人的临床效果研究

目的 探究自拟中药方717解毒合剂治疗蝮蛇咬伤病人的临床效果。方法 选取2018年9月至2021年6月中国人民解放军联勤保障部队第九一〇医院收治的68例被蝮蛇咬伤的病人,根据随机数字表法分为观察组和对照组。其中对照组采用常规西药治疗(抗蝮蛇毒血清6 000 U加入0.9%氯化钠注射液250 mL静脉滴注),观察组在常规西药治疗的基础上加服自拟中药方717解毒合剂,每组34例。比较两组病人的临床疗效。结果 观察组病人治愈人数23例(占比67.65%),总有效率为91.18%;对照组治愈人数16例(占比47.06%),总有效率为82.35%;观察组治愈人数显著多于对照组(P<0.05);经治疗后,观察组病人急性生理学和慢性健康状况评价Ⅱ(APACHE Ⅱ)评分明显优于对照组(P<0.05)。治疗前,两组病人血清乳酸脱氢酶(LDH)、肌酸激酶及肌酸激酶同工酶(CK-MB)的含量相比较差异无统计学意义(P>0.05);经治疗后,随着治疗天数的增加,各指标出现明显好转,并且观察组改善效果显著优于对照组(P<0.05)。治疗前,两组病人肾损伤相关因子血清肌酐、血浆中中性粒细胞明胶酶相关脂质运载蛋白(pNGAL)、尿液中中性粒细胞明胶酶相关脂质运载蛋白(uNGAL)含量相比较差异无统计学意义(P>0.05);经治疗后,随着治疗天数的增加,各指标出现明显好转,并且观察组改善效果显著优于对照组(P<0.05)。结论717解毒合剂联合西医治疗蝮蛇咬伤后病人的临床治疗效果相比于单独采用西医治疗更显著,能够快速降低病人血清内的毒素,减少毒素对于心肌细胞的损伤以及肾损伤,值得临床推广。

白术炮制措施对中药饮片治疗溃疡性结肠炎效果的影响研究

目的研究白术炮制措施对中药饮片治疗溃疡性结肠炎效果的影响。方法回顾性分析汕头潮南民生医院于2021年2月—2022年11月接收的99例溃疡性结肠炎(ulcerative colitis,UC)患者临床资料,并根据服用不同的中药炮制方式制成的中药饮片分为三组(A组:土炒处理分级法;B组:焦制分级法;C组:炙制分级法),各33例。对比三组的药物疗效及不良药物反应(adverse drug reaction,ADR)。结果三组UA患者的疗效各不相同,B组的治疗效果比A稍高,但两组的疗效结果对比,差异无统计学意义(χ^(2)=0.244,P=0.621);C组的UC患者服用经水处理前联合炮制综合法煎制的药液的总体治疗效果比A组患者的疗效佳,差异有统计学意义(χ^(2)=17.827,P=0.001);C组的UC患者服用经水处理前联合炮制综合法煎制的药液的总体治疗效果比B组患者的疗效佳,差异有统计学意义(χ^(2)=14.580,P=0.001)。三组的ADR发生率均有所不同,其中A组的ADR比例值与B组相近,差异无统计学意义(χ^(2)=0.096,P=0.757);C组的ADR发生率似乎低于其他两组,但差异无统计学意义(C/A:χ^(2)=1.158,P=0.282;C/B:χ^(2)=1.886,P=0.170)。结论将中药加工与预处理结合使用似乎可以提高中药的疗效,且不会对用药安全产生影响。