期刊文献+

二次检索

题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息
共找到7,495篇文章
< 1 2 250 >
每页显示 20 50 100
Role of exosomal circular RNAs as microRNA sponges and potential targeting for suppressing hepatocellular carcinoma growth and progression 被引量:2
1
作者 Nikolaos Papadopoulos Eleni-Myrto Trifylli 《World Journal of Gastroenterology》 SCIE CAS 2024年第9期994-998,共5页
In this editorial,we comment on the article by Lyu et al published in the recent issue of the World Journal of Gastroenterology(2023;2219-2840).Hepatocellular carcinoma(HCC)is a frequently encountered and highly aggre... In this editorial,we comment on the article by Lyu et al published in the recent issue of the World Journal of Gastroenterology(2023;2219-2840).Hepatocellular carcinoma(HCC)is a frequently encountered and highly aggressive primary liver cancer,which remains the third-commonest cause of cancer-related death despite the current therapeutic modalities.There is urgency in developing novel thera-peutic approaches,such as by manipulating extracellular vesicles,which con-stitute a highly heterogeneous nanoparticle population that contains various cargoes.These cargoes have a pivotal role in cell-to-cell communication and can modify the functional level of the recipient cells via their uptake by other recipient cells.Exosomal non-coding RNAs have particular evolving significance in HCC,such as circular RNAs,which have been found differentially expressed in normal hepatic and HCC tissues.The aberrations in their expression levels have a key role in the HCC development and progression and the overall prognosis.In this editorial,we will shed light on the emerging role of exosomal circular RNAs in HCC development and progression,focusing on the oncogenic or potentially tumor suppressive effect of mesenchymal stem cells-derived exosomal non-coding RNAs. 展开更多
关键词 CIRCULAR sponge PROGRESSION
下载PDF
Retraction:long noncoding RNA ANRIL promotes cervical cancer development by acting as a sponge of miR-186
2
作者 Oncology Research Editorial Office 《Oncology Research》 SCIE 2024年第11期1817-1817,共1页
Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed ... Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells. 展开更多
关键词 CERVICAL sponge SIMILARITY
下载PDF
Performance improvement in stepped solar still modified by sponge layer
3
作者 Mahtab TARAHOMI Saman RASHIDI +1 位作者 Faramarz HORMOZI Shahabeddin ASHTIANI 《Journal of Central South University》 SCIE EI CAS CSCD 2024年第6期1973-1982,共10页
In this paper,the experimental investigation on the performance improvement of conventional stepped solar still is conducted.The steps are covered by the porous material to improve the performance of the conventional ... In this paper,the experimental investigation on the performance improvement of conventional stepped solar still is conducted.The steps are covered by the porous material to improve the performance of the conventional device and increase the evaporation rate.All the parameters,including the temperature on the glass surface,the water temperature inside the evaporation zone,and the amount of water produced in both conventional and modified stepped solar stills are measured and compared.The efficiency of two devices and their exergy efficiency have been calculated.Finally,the economic analysis of both devices has been done to check the economic feasibility of the modified device.The amount of freshwater generated during one day was 2244.4 and 3076.2 mL/m^(2),respectively for the conventional and modified stepped solar stills.As a result,the amount of water produced in one day by modified stepped solar still is 35.5% more than the conventional one.Also,the costs for the conventional and modified stepped solar stills have been calculated as 0.0359 and 0.029$/(L·m^(-2)),respectively. 展开更多
关键词 stepped solar still black sponge EFFICIENCY EXERGY economic analysis
下载PDF
A green cross-linking method for the preparation of renewable threedimensional graphene sponges for efficient adsorption of Congo red dye
4
作者 Zhuang Liu Bo Gao +3 位作者 Haoyuan Han Yuling Li Haiyang Fu Donghui Wei 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2024年第2期84-93,共10页
Graphene-based materials possess significant potential for the treatment of dye wastewater due to their exceptional adsorption properties toward stubborn pollutants.However,their utilization is hindered by high prepar... Graphene-based materials possess significant potential for the treatment of dye wastewater due to their exceptional adsorption properties toward stubborn pollutants.However,their utilization is hindered by high preparation costs,low yields,environmental pollution during synthesis,and challenges in regenerating the adsorbent.This study proposes a novel approach to address these limitations by developing nitrogen-doped three-dimensional(3D)polyvinyl alcohol(PVA)crosslinked graphene sponges(N-PGA)using a cross-linking method with ammonium carbonate.This method offers a relatively mild,environmentally friendly approach.Ammonium carbonate serves as both a reducing and modifying agent,facilitating the formation of the intrinsic structure of N-PGA and acting as a nitrogen source.Meanwhile,PVA is utilized as the cross-linking agent.The results demonstrate that N-PGA exhibits a favorable internal 3D hierarchical porous structure and possesses robust mechanical properties.The measured specific surface area(BET)of N-PGA was as high as406.538 m^(2)·g^(-1),which was favorable for its efficient adsorption of Congo red(CR)dye molecules.At an initial concentration of 50 mg·L^(-1),N-PGA achieved an impressive removal rate of 89.6%and an adsorption capacity of 112 mg·g^(-1)for CR dye.Furthermore,it retained 79%of its initial adsorption capacity after 10 cycles,demonstrating excellent regeneration performance.In summary,the synthesized N-PGA displays remarkable efficacy in the adsorption of CR dye in wastewater,opening up new possibilities for utilizing 3D porous graphene nanomaterials as efficient adsorbents in wastewater treatment. 展开更多
关键词 Green chemistry Graphene sponge Adsorbents ADSORPTION Congo red Regeneration
下载PDF
Efficient removal of U(VI) from wastewater by a sponge-like 3D porous architecture with hybrid electrospun nanofibers
5
作者 Lin Hu Lin Chen +4 位作者 Xian-kun Wu Rui Luo Rong-guan Lv Zheng-hao Fei Feng Yang 《Water Science and Engineering》 EI CAS CSCD 2024年第2期150-156,共7页
Removal of uranium(VI)from nuclear wastewater is urgent due to the global nuclear energy exploitation.This study synthesized novel sponge-like 3D porous materials for enhanced uranium adsorption by combining electrosp... Removal of uranium(VI)from nuclear wastewater is urgent due to the global nuclear energy exploitation.This study synthesized novel sponge-like 3D porous materials for enhanced uranium adsorption by combining electrospinning and fibrous freeze-shaping techniques.The materials possessed an organic-inorganic hybrid architecture based on the electrospun fibers of polyacrylonitrile(PAN)and SiO_(2).As a sup-porting material,the surface of fibrous SiO_(2) could be further functionalized by cyano groups via(3-cyanopropyl)triethoxysilane.All the cyano groups were turned into amidoxime(AO)groups to obtain a amidoxime-functionalized sponge(PAO/SiO_(2)-AO)through the subsequent ami-doximation process.The proposed sponge exhibited enhanced uranium adsorption performance with a high removal capacity of 367.12 mg/g,a large adsorption coefficient of 4.0×10^(4)mL/g,and a high removal efficiency of 97.59%.The UO_(2)^(2+)adsorption kinetics perfectly conformed to the pseudo-second-order reaction.The sorbent also exhibited an excellent selectivity for UO_(2)^(2+) with other interfering metal ions.2023 Hohai University.Production and hosting by Elsevier B.V. 展开更多
关键词 Uranium removal sponge Amidoximation Organic-inorganic hybrid structure ELECTROSPINNING
下载PDF
LncRNAs unraveling their sponge role in glioblastoma and potential therapeutic applications
6
作者 CAIJUAN LIU XINGHAO LI +3 位作者 YUXUAN WU JINHUI YANG MENGHAN WANG YUNQI MA 《BIOCELL》 SCIE 2024年第3期387-401,共15页
Glioblastoma multiforme(GBM),the most common and aggressive primary brain tumor in adults,is the most malignant and still has no cure.However,the novel role of long non-coding RNAs(lncRNAs)in the pathogenesis of gliob... Glioblastoma multiforme(GBM),the most common and aggressive primary brain tumor in adults,is the most malignant and still has no cure.However,the novel role of long non-coding RNAs(lncRNAs)in the pathogenesis of glioblastoma is attracting extensive attention.LncRNAs are transcribed RNA molecules over 200 nucleotides long that do not encode proteins.Unlike small non-coding RNAs,such as microRNAs(miRNAs),lncRNAs have more complex secondary and tertiary structures that enable them to interact with DNA,RNA,and proteins and perform multiple regulatory functions.LncRNAs act as molecular sponges,absorbing and sequestering other biomolecules,particularly miRNAs,thereby preventing these molecules from performing their normal functions.LncRNAs influence glioblastoma through gene expression regulation,molecular sponge capacity,epigenetic modulation,and signaling pathway interactions.In glioblastoma,a large number of lncRNAs have been found to be abnormally expressed,affecting tumor growth,invasion and resistance to treatment.Due to its regulatory role and disease-specific expression patterns,lncRNA has become a potential biomarker for glioblastoma and a promising new therapeutic target.This paper discusses the spongy role of lncRNAs in glioblastoma and its potential therapeutic applications,which will lay a foundation for our understanding of glioblastoma biology and the development of new diagnostic and therapeutic strategies in the future. 展开更多
关键词 LncRNA MIRNA BIOMARKER Molecular sponges Tumor microenvironment
下载PDF
Physical property improvement of fluid shortening using peanut oil-based diacylglycerols and their applications in sponge cake
7
作者 Yilin Du Shuo Zou +2 位作者 Yee-Ying Lee Yong Wang Zhen Zhang 《Grain & Oil Science and Technology》 CAS 2024年第3期150-158,共9页
Fluid shortening is an important ingredient in the production of sponge cake. Peanut oil with 0, 43% and 85% of diacylglycerol content was used as the base oil. Different emulsifiers, such as glycerol monostearate, so... Fluid shortening is an important ingredient in the production of sponge cake. Peanut oil with 0, 43% and 85% of diacylglycerol content was used as the base oil. Different emulsifiers, such as glycerol monostearate, soy lecithin and sucrose ester, and their respective amounts, were investigated. It was found that the addition of emulsifiers had a positive effect on water-absorbing capacity, air-absorbing capacity and viscosity of the oils. Glycerol monostearate was the preferred emulsifier for fluid shortening with a recommended addition of 1.5%. The effects of different diacylglycerol content on fluid shortening and their impact on sponge cake production was also investigated. The onset oxidation temperature of the oil could be increased from 253.21 ℃ for PO-TAG-based fluid shortening to 263.70 ℃ for PO-DAG85-based fluid shortening. And the increase in diacylglycerol content leading to a lower specific gravity of the batter, which was 1.06 g/mL, 1.02 g/mL and 0.98 g/mL prepared by PO-DAG, PO-DAG43 and PO-DAG85 shortening, respectively. The results showed that diacylglycerols can be used as base oils in fluid shortening to improve the crystal network and stability of fluid shortenings, thereby reducing the specific gravity of the batter and improving the structural properties of the cake. This will extend the potential applications of diacylglycerols and increase the variety of base oils available for fluid shortening preparation. 展开更多
关键词 Fluid shortening DIACYLGLYCEROL EMULSIFIER sponge cake
下载PDF
Superhydrophobic melamine sponge prepared by radiation-induced grafting technology for efficient oil-water separation
8
作者 Ying Sun Wen-Rui Wang +7 位作者 Dan-Yi Li Si-Yi Xu Lin Lin Man-Li Lu Kai Fan Chen-Yang Xing Lin-Fan Li Ji-Hao Li 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2024年第9期103-114,共12页
This paper presents a superhydrophobic melamine(ME)sponge(ME-g-PLMA)prepared via high-energy radiation-induced in situ covalent grafting of long-alkyl-chain dodecyl methacrylate(LMA)onto an ME sponge for efficient oil... This paper presents a superhydrophobic melamine(ME)sponge(ME-g-PLMA)prepared via high-energy radiation-induced in situ covalent grafting of long-alkyl-chain dodecyl methacrylate(LMA)onto an ME sponge for efficient oil–water separation.The obtained ME-g-PLMA sponge had an excellent pore structure with superhydrophobic(water contact angle of 154°)and superoleophilic properties.It can absorb various types of oils up to 66–168 times its mass.The ME-g-PLMA sponge can continuously separate oil slicks in water by connecting a pump or separating oil underwater with a gravity-driven device.In addition,it maintained its highly hydrophobic properties even after long-term immersion in different corrosive solutions and repeated oil adsorption.The modified ME-g-PLMA sponge exhibited excellent separation properties and potential for oil spill cleanup. 展开更多
关键词 Radiation-induced graft polymerization Oil–water separation sponge SUPERHYDROPHOBIC
下载PDF
Ultrahydrophobic melamine sponge via interfacial modification with reduced graphene oxide/titanium dioxide nanocomposite and polydimethylsiloxane for oily wastewater treatment
9
作者 Hamidatu Alhassan Ying Woan Soon +1 位作者 Anwar Usman Voo Nyuk Yoong 《Water Science and Engineering》 EI CAS CSCD 2024年第2期139-149,共11页
Three-dimensional(3D)porous absorbents have attracted significant attention in the oily wastewater treatment technology due to their high porosity and elasticity.Given their amphiphilic surface,they have a propensity ... Three-dimensional(3D)porous absorbents have attracted significant attention in the oily wastewater treatment technology due to their high porosity and elasticity.Given their amphiphilic surface,they have a propensity to simultaneously absorb water and oil,which restricts their range of applications.In this study,a reduced graphene oxide and titanium dioxide nanocomposite(rGO/TiO_(2))was used to fabricate an ultra-hydrophobic melamine sponge(MS)through interfacial modification using a solution immersion technique.To further modify it,poly-dimethylsiloxane(PDMS)was grafted onto its surface to establish stronger covalent bonds with the composite.The water contact angle of the sponge(rGO/TiO_(2)/PDMS/MS)was 164.2°,which satisfies the condition for ultrahydrophobicity.The evidence of its water repellency was demonstrated by the Cassie-Baxter theory and the lotus leaf effect.As a result of the increased density of rGO/TiO_(2)/PDMS/MS,it recorded an initial capacity that was 2 g/g lower than the raw MS for crude oil absorption.The raw MS retained 53% of its initial absorption capacity after 20 cycles of absorption,while rGO/TiO_(2)/PDMS/MS retained 97%,suggesting good recyclability.Excellent oil and organic solvent recovery(90%-96%)was demonstrated by rGO/TiO_(2)/PDMS/MS in oil-water combinations.In a continuous separation system,it achieved a remarkable separation efficiency of 2.4×10^(6)L/(m^(3)·h),and in turbulent emulsion separation,it achieved a demulsification efficiency of 90%-91%.This study provides a practical substitute for massive oil spill cleaning. 展开更多
关键词 Oily wastewater Reduced graphene oxide Polydimethylsiloxane(PDMS) Emulsion separation Melamine sponge
下载PDF
Characterization of Flame Retardancy and Oil-Water Separation Capacity of Superhydrophobic Silylated Melamine Sponges
10
作者 Yongchun Liu Ni Qiao +7 位作者 Yanli Yang Yanchun Li Chunxiao He Siyang Wang Chengcheng Liu Ruixia Lei Wang Li Wenwen Gao 《Fluid Dynamics & Materials Processing》 EI 2024年第2期383-400,共18页
A silylated melamine sponge(SMS)was prepared by two simple steps,namely,immersion and dehydration of a melamine sponge coated with methyltrichlorosilane.The silylated structure of SMS was characterized by FT-IR(Fourie... A silylated melamine sponge(SMS)was prepared by two simple steps,namely,immersion and dehydration of a melamine sponge coated with methyltrichlorosilane.The silylated structure of SMS was characterized by FT-IR(Fourier-transform infrared)spectroscopy,SEM(Scanning electron microscopy)and in terms of water contact angles.Its oil-water absorption and separation capacities were measured by FT-IR and UV-visible spectrophoto-metry.The experimental results have shown that oligomeric silanol covalently bonds by Si-N onto the surface of melamine sponge skeletons.SMS has shown superhydrophobicity with a water contact angle exceeding 150°±1°,a better separation efficiency with regard to diesel oil(by 99.31%(wt/wt%)in oil-water mixture and even up to 99.99%(wt/wt%)for diesel oil in its saturated aqueous solution.Moreover,SMS inherited the intrinsicflame retardancy of the melamine sponge.In general,SMS has shown superhydrophobicity,high porosity,excellent selectivity,remarkable recyclability,and better absorption capacity for various oils and organic solvents,and a high separation efficiency for oil in saturated aqueous solutions. 展开更多
关键词 METHYLTRICHLOROSILANE silylated melamine sponge(SMS) SUPERHYDROPHOBICITY absorption capacity oil-water separable efficiency flame retardancy
下载PDF
基于Sponge结构的轻量级Hash函数设计 被引量:2
11
作者 赵太飞 尹航 李永明 《小型微型计算机系统》 CSCD 北大核心 2018年第12期2636-2639,共4页
随着物联网技术的高速发展、资源受限设备的广泛应用,传统密码算法正面临着新的挑战.除了要提供一定程度的安全保证外,资源受限环境下的Hash函数还面临着轻量化需求.提出一种基于Sponge结构的轻量级Hash函数,内部变换采用改进的Klein算... 随着物联网技术的高速发展、资源受限设备的广泛应用,传统密码算法正面临着新的挑战.除了要提供一定程度的安全保证外,资源受限环境下的Hash函数还面临着轻量化需求.提出一种基于Sponge结构的轻量级Hash函数,内部变换采用改进的Klein算法,并对其软件实现进行面对字节的优化,具有64位摘要长度.对算法进行依赖性测试和安全分析结果表明该算法可以满足资源受限环境的安全需求.效率测试表明该算法硬件实现规模满足资源受限环境的要求,并兼顾了软件实现效率. 展开更多
关键词 HASH 轻量级 sponge KLEIN
下载PDF
Landscape Design Methods of Urban Sponge Park 被引量:2
12
作者 陈晓刚 朱智 杨昆 《Agricultural Science & Technology》 CAS 2016年第4期938-941,共4页
Urban rain-caused floods can not be well resolved with rising urban problem. Hence, construction of sponge park is born at the right moment. The research reviewed the concept, content and value of sponge parks and pro... Urban rain-caused floods can not be well resolved with rising urban problem. Hence, construction of sponge park is born at the right moment. The research reviewed the concept, content and value of sponge parks and proposed theory and principle of landscape design in order to seek the method of landscape design of sponge parks, improve urban eco-environment and advance harmony development between human and nature. 展开更多
关键词 sponge park Low impact development Design method
下载PDF
真核表达载体pEGFP-C2-miR-126-sponge的构建及其表达活性研究 被引量:6
13
作者 胡燕 廖珍媛 +4 位作者 李永菊 陈超 朱顺飞 熊思东 徐林 《第三军医大学学报》 CAS CSCD 北大核心 2014年第1期33-37,共5页
目的构建靶向miR-126的真核表达载体pEGFP-C2-miR-126-海绵体(sponge),并探讨其下调miR-126的表达对肝癌HepG2细胞体外生长的影响,为后续深入研究miR-126在肝癌发生中的作用提供前期实验基础。方法合成miR-126-sponge,构建pEGFP-C2-miR-... 目的构建靶向miR-126的真核表达载体pEGFP-C2-miR-126-海绵体(sponge),并探讨其下调miR-126的表达对肝癌HepG2细胞体外生长的影响,为后续深入研究miR-126在肝癌发生中的作用提供前期实验基础。方法合成miR-126-sponge,构建pEGFP-C2-miR-126-sponge真核表达载体,体外瞬时转染肝癌细胞HepG2,荧光显微镜下观察EGFP的表达情况;Real-time PCR检测细胞中miR-126的表达水平;MTT法和克隆形成实验检测细胞的增殖变化;划痕实验观察细胞的体外迁移能力改变。结果成功构建pEGFP-C2-miR-126-sponge真核表达载体(命名为p-miR-126-sp);Real-time PCR结果显示,与对照组(p-Cont)相比,p-miR-126-sp组中miR-126表达水平显著降低(P<0.05);MTT检测发现,p-miR-126-sp组中细胞增殖数目明显减少(P<0.05);此外,p-miR-126-sp组的克隆形成数和细胞迁移数均明显减少(P<0.05)。结论 miR-126-sponge可显著下调miR-126的表达水平进而抑制肝癌细胞HepG2的体外增殖及迁移能力。 展开更多
关键词 miR-126-sponge 肝癌 HEPG2细胞 细胞增殖
下载PDF
Ceramide from the Marine Sponge Phyllospongia sp 被引量:1
14
作者 万一千 苏镜娱 曾陇梅 《中山大学学报(自然科学版)》 CAS CSCD 北大核心 1997年第5期113-115,共3页
从中国南海海绵Phylospongiasp.中分离得到了一个神经酰胺类化合物Phyl-loamideA,它的结构通过波谱方法和化学方法确定.
关键词 海绵 神经酰胺 怀叶海绵 杯叶酰胺A
下载PDF
基于新加密结构和Sponge结构的轻量级Hash函数CHF
15
作者 黄玉划 代学俊 +3 位作者 陈帮春 苏菲 刘宁钟 曾庆喜 《南京航空航天大学学报》 EI CAS CSCD 北大核心 2016年第5期662-667,共6页
针对能耗等资源受限环境对密码算法的需求,基于Sponge迭代结构,采用基于新加密结构(命名为MS结构)的CLEFIA-128*(轻量级分组密码国际标准的修订算法)作为压缩函数,设计了一个轻量级Hash函数CHF。效率测试和分析表明CHF算法的软件效率高... 针对能耗等资源受限环境对密码算法的需求,基于Sponge迭代结构,采用基于新加密结构(命名为MS结构)的CLEFIA-128*(轻量级分组密码国际标准的修订算法)作为压缩函数,设计了一个轻量级Hash函数CHF。效率测试和分析表明CHF算法的软件效率高于常见轻量级Hash函数,并兼顾了硬件效率,既能满足射频识别(Radio frequency identification,RFID)等资源极端受限环境对硬件的使用需求,也可以满足其他一些诸如嵌入式系统和单片机等环境对软件实现的需求,适用范围更广。依赖性测试和安全分析表明,该算法能够满足轻量级Hash函数的安全需求,也从侧面论证了MS结构的安全性。 展开更多
关键词 轻量级Hash函数 sponge结构 CLEFIA算法 依赖性测试 密码分析
下载PDF
pG-miR-7-Sponge转基因小鼠载体的构建及鉴定 被引量:4
16
作者 李永菊 周涯 +5 位作者 陈超 朱顺飞 胡燕 秦娜琳 郑静 徐林 《遵义医学院学报》 2014年第2期156-160,共5页
目的构建pG-miR-7-Sponge转基因小鼠的真核载体并检测其表达活性,为转基因小鼠的建立提供前期实验基础。方法利用miRNA sponge(海绵体)技术原理,合成miR-7-Sponge序列,酶切连接入真核表达载体eGFP-C2并转化Stbl3感受态细胞,克隆PCR、酶... 目的构建pG-miR-7-Sponge转基因小鼠的真核载体并检测其表达活性,为转基因小鼠的建立提供前期实验基础。方法利用miRNA sponge(海绵体)技术原理,合成miR-7-Sponge序列,酶切连接入真核表达载体eGFP-C2并转化Stbl3感受态细胞,克隆PCR、酶切和测序鉴定重组载体eGFP-C2-miR-7-Sponge(简称pG-miR-7-Sp);将重组载体pG-miR-7-Sp体外瞬时转染人肺癌95D细胞,荧光显微镜下观察细胞的GFP表达情况;Real-Time PCR检测细胞中miRNA-7的表达;MTT法检测细胞的增殖变化。结果经克隆PCR、酶切鉴定及测序分析,成功构建了真核表达载体pGmiR-7-Sp;pG-miR-7-Sp瞬时转染95D细胞48 h后,与对照组细胞相比,miR-7表达水平明显降低(P<0.05);同时细胞的生长明显加快(P<0.05)。结论成功构建pG-miR-7-Sp真核表达载体。 展开更多
关键词 微小RNA-7 海绵体 95D细胞 载体构建 基因表达
下载PDF
miRNA Sponge介导的miR-17和miR-20a基因沉默的抗白血病作用机制 被引量:2
17
作者 牛文艳 吴顺泉 +2 位作者 徐珍珍 林君 战榕 《中国实验血液学杂志》 CAS CSCD 北大核心 2014年第4期932-937,共6页
本研究旨在检测急性白血病患者中miR-17和miR-20a前体的表达水平,并探讨miRNA Sponge介导的miR-17和miR-20a沉默的抗白血病作用机制。采用荧光实时定量PCR方法检测初治急性白血病患者及8种白血病细胞株中miR-17和miR-20a前体的表达水平... 本研究旨在检测急性白血病患者中miR-17和miR-20a前体的表达水平,并探讨miRNA Sponge介导的miR-17和miR-20a沉默的抗白血病作用机制。采用荧光实时定量PCR方法检测初治急性白血病患者及8种白血病细胞株中miR-17和miR-20a前体的表达水平,分析其在各型白血病中的表达情况。利用前期构建的针对miR-17和miR-20a基因的miRNA Sponge慢病毒表达载体,感染高表达miR-17和miR-20a的Jurkat细胞株;用CCK-8方法及流式细胞术分别检测miR-17和miR-20a沉默对Jurkat细胞增殖能力及细胞周期的影响。结果表明:初治急性白血病患者中miR-17和miR-20a前体的表达明显高于正常对照(P<0.05);且与急性髓系白血病患者相比,急性淋巴细胞白血病患者中的表达量更高;然而二者表达量与患者外周血高白细胞计数无明显相关性(P>0.05)。miR-17和miR-20a沉默能抑制Jurkat细胞的增殖,使细胞周期阻滞于G1—S期,同时促进细胞的凋亡。结论:急性白血病患者中miR-17和miR-20a呈过表达,可能参与白血病的发生、发展;高表达的miR-17和miR-20a可通过在转录后抑制P21和E2F1表达,从而促进细胞增殖及细胞周期G1—S期转换,抑制细胞凋亡。 展开更多
关键词 MIRNA sponge miR-17 miR-20a 基因沉默 抗白血病机制
下载PDF
构建稳定表达pLVshRNA-EGFP-EBVsponge的SNU719细胞系 被引量:2
18
作者 陈香岭 杨梦莉 +1 位作者 马春霞 俞建昆 《贵州医科大学学报》 CAS 2017年第4期435-439,共5页
目的:构建稳定表达pLVshRNA-EGFP-EBVsponge的SNU719细胞系。方法:合成能与EB病毒(EBV)编码的EBV-miR-BART16、EBV-miR-BART4-5p、EBV-miR-BART5-5p及EBV-miR-BART22 miRNAs特异结合的miRNA海绵(miRNA sponge)序列,用PITA软件预测构建的... 目的:构建稳定表达pLVshRNA-EGFP-EBVsponge的SNU719细胞系。方法:合成能与EB病毒(EBV)编码的EBV-miR-BART16、EBV-miR-BART4-5p、EBV-miR-BART5-5p及EBV-miR-BART22 miRNAs特异结合的miRNA海绵(miRNA sponge)序列,用PITA软件预测构建的sponge是否结合非特异性miRNAs;构建pLVshRNA-EGFP-EBVsponge载体,与慢病毒包装质粒pH1、pH2共转染293T细胞,包装产生慢病毒颗粒,感染SNU719细胞,经嘌呤霉素筛选获得稳定表达细胞株;Real-time PCR检测细胞中EBV-miR-BART16、EBV-miRBART4-5p、EBV-miR-BART5-5p及EBV-miR-BART22表达水平。结果:成功构建pLVshRNA-EGFP-EBVspong(命名为pEGFP-EBV-sp);与对照组相比,pEGFP-EBV-sp组中4种microRNA表达水平显著降低,差异有统计学意义(P<0.05)。结论:成功构建稳定表达pEGFP-EBV-sp的SNU719细胞系。 展开更多
关键词 细胞系 病毒 胃肿瘤 细胞凋亡 MIRNA sponge SNU719
下载PDF
Development of Tuckahoe (Poria cocos) Purple Potato Steamed Sponge Cake 被引量:3
19
作者 江山 刘丽 +2 位作者 邹成梅 张诗泉 吴鹏 《Agricultural Science & Technology》 CAS 2017年第6期1080-1082,1105,共4页
This study was conducted to find the optimum process conditions for tuck- ahoe (Poria cocos) purple potato steamed sponge cake. The results showed that the best production process conditions of tuckahoe purple potat... This study was conducted to find the optimum process conditions for tuck- ahoe (Poria cocos) purple potato steamed sponge cake. The results showed that the best production process conditions of tuckahoe purple potato steamed sponge cake were as follows: ratio of wheat flour to tuckahoe power at 8:2, 10% purple potato starch, 25% white granulated sugar, and 1.5% yeast powder. 展开更多
关键词 Tuckahoe powder Purple potato starch Steamed sponge cake Opti- mum process
下载PDF
Waterfront Spatial Design from the Perspective of Sponge City 被引量:2
20
作者 陈坤 《Agricultural Science & Technology》 CAS 2017年第6期1095-1096,1125,共3页
Due to environmental awareness, demand for watercourse landscape eco- function is growing domestically. However, flood control and drainage are the only focuses, and the rest functions of water course are slighted. Be... Due to environmental awareness, demand for watercourse landscape eco- function is growing domestically. However, flood control and drainage are the only focuses, and the rest functions of water course are slighted. Besides, cross-section biodiversity is falling and wetland self-cleaning capacity is impairing, resulting from river limitation on riverbed, making a straight river bend and revetment. So, water course has to be managed from ecology, economy, culture and social effect, to re- store natural river function and meet human demands. And to return to nature and people first would be the general principles. 展开更多
关键词 sponge city Waterfront area Planning and designing
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部