Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breedin...Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breeding and cultivation to improve nutritional quality.However,little information is available on differences in lysine anabolism between SG and IG and the underlying mechanism,and whether and how irrigation regimes affect lysine anabolism in these grains.A japonica rice cultivar was grown in the field and two irrigation regimes,continuous flooding(CF)and wetting alternating with partial drying(WAPD),were imposed from heading to the mature stage.Lysine content and activities of key enzymes of lysine biosynthesis,and levels of brassinosteroids(BRs)were lower in the IG than in the SG at the early grainfilling stage but higher at middle and late grain-filling stages.WAPD increased activities of these key enzymes,BR levels,and contents of lysine and total amino acids in IG,but not SG relative to CF.Application of 2,4-epibrassinolide to rice panicles in CF during early grain filling reproduced the effects of WAPD,but neither treatment altered the activities of enzymes responsible for lysine catabolism in either SG or IG.WAPD and elevated BR levels during grain filling increased lysine biosynthesis in IG.Improvement in lysine biosynthesis in rice should focus on IG.展开更多
The typical loess on high slopes along the BaoLan High-speed Rail, China, was selected as the research object. The influence of the freeze-thaw cycle and dry-wet alternation on the shear-strength parameters of the uns...The typical loess on high slopes along the BaoLan High-speed Rail, China, was selected as the research object. The influence of the freeze-thaw cycle and dry-wet alternation on the shear-strength parameters of the unsaturated loess was investigated by laboratory experimental methods. Moreover, the temperature field, seepage field, and stability of slopes with different gradients were simulated under the effect of the freeze-thaw cycle and dry-wet alternation by using the geotechnical analysis software Geo-Studio. The research results showed(1) when the freeze-thaw cycle was repeated on the slope, with the frozen depth increasing, the melted depth did the same; besides, the closed loop of isotherms formed on the slope;(2) under the action of dry-wet circulation, the negative pore-water pressure and volumetric water content showed an upward tendency. However, owing to the different slope gradients, rainfall infiltration was not the same. As time went by, the differences of the negative pore-water pressure and volumetric water content between the slopes of different gradients continued to increase;(3) with the freeze-thaw cycle and dry-wet alternation increasing, the slope-safety factor decreased. Especially in the early period, the slope-safety factor changed remarkably. For slopes undergoing freeze-thaw action, the slope-safety factor was negatively correlated with the gradient. However, with regard to slopes undergoing dry-wet alternation, the result became more complex because the slope-safety factor was related to both seepage strength and slope grade. Accordingly, further research is needed to study the effect of seepage strength and slope grade on the stability of loess slopes.展开更多
Alternate wetting and drying(AWD)system,in which water has been reduced by approximately 35%with an increased occurrence of beneficial arbuscular mycorrhizal(AM)symbiosis and no negative impact on rice yield,was propo...Alternate wetting and drying(AWD)system,in which water has been reduced by approximately 35%with an increased occurrence of beneficial arbuscular mycorrhizal(AM)symbiosis and no negative impact on rice yield,was proposed to utilize water and nutrients more sustainable.In this study,we selected six rice cultivars(Centauro,Loto,Selenio,Vialone nano,JSendra and Puntal)grown under AWD conditions,and investigated their responsiveness to AM colonization and how they select diverse AM taxa.In order to investigate root-associated AM fungus communities,molecular cloning-Sanger sequencing on small subunit rDNA data were obtained from five out of the six rice cultivars and compared with Next Generation Sequencing(NGS)data,which were previously obtained in Vialone nano.The results showed that all the cultivars were responsive to AM colonization with the development of AM symbiotic structures,even if with differences in the colonization and arbuscule abundance in the root systems.We identified 16 virtual taxa(VT)in the soil compartment and 7 VT in the root apparatus.We emphasized that the NGS analysis gives additional value to the results thanks to a more in-depth reading of the less represented AM fungus taxa.展开更多
This study investigated if super rice could better cope with soil water deficit and if it could have better yield performance and water use efficiency (WUE) under alternate wetting and drying (AWD) irrigation than...This study investigated if super rice could better cope with soil water deficit and if it could have better yield performance and water use efficiency (WUE) under alternate wetting and drying (AWD) irrigation than check rice. Two super rice cultivars and two elite check rice cultivars were grown in pots with three soil moisture levels, well watered (WW), moderate water deficit (MWD) and severe water deficit (SWD). Two cultivars, each for super rice and check rice, were grown in field with three irrigation regimes, alternate wetting and moderate drying (AWMD), alternate wetting and severe drying (AWSD) and conventional irrigation (CI). Compared with that under WW, grain yield was significantly decreased under MWD and SWD treatments, with less reduction for super rice than for check rice. Super rice had higher percentage of productive tillers, deeper root distribution, higher root oxidation activity, and greater aboveground biomass production at mid and late growth stages than check rice, especially under WMD and WSD. Compared with CI,AWMD increased, whereasAWSD decreased grain yield, with more increase or less decrease for super rice than for check rice. Both MWD and SWD treatments and eitherAWMD orAWSD regime significantly increased WUE compared with WW treatment or CI regime, with more increase for super rice than for check rice. The results suggest that super rice has a stronger ability to cope with soil water deficit and holds greater promising to increase both grain yield and WUE by adoption of moderate AWD irrigation.展开更多
Direct seeded rice is promising alternative to traditional transplanting, but requires appropriate crop and water management to maintain yield performance and achieve high water productivity. Present study evaluated t...Direct seeded rice is promising alternative to traditional transplanting, but requires appropriate crop and water management to maintain yield performance and achieve high water productivity. Present study evaluated the effect of seed priming and irrigation on crop establishment, tillering, agronomic traits, paddy yield, grain quality and water productivity of direct seeded rice in alternate wetting and drying (DSR-AWD) in comparison with direct seeded rice at field capacity (DSR- FC). Seed priming treatments were osmo-priming with KCI (2.2%), CaCI2 (2.2%) and moringa leaf extracts (MLE, 3.3%) including hydro-priming as control. Among the treatments, seed osmo-primed with MLE emerged earlier and had higher final emergence, followed by osmo-priming with CaCI2. Tillering emergence rate and number of tillers per plant were the highest for seed priming with CaCI2 in DSR- AWD. Total productive and non-productive tillers, panicle length, biological and grain yields, harvest index were highest for seed priming with MLE or CaCI2 in DSR-AWD. Similarly, grain quality, estimated in terms of normal grains, abortive and chalky grains, was also the highest in DSR-AWD with MLE osmo-priming. Benefit cost ratio and water productivity was also the highest in DSR-AWD for seed priming with MLE. In conclusion, seed priming with MLE or CaCI2 can be successfully employed to improve the direct seeded rice performance when practiced with alternate wetting and drying irrigation.展开更多
Compared to drought-susceptible rice cultivars(DSRs),drought-resistance rice cultivars(DRRs)could drastically reduce the amount of irrigation water input and simultaneously result in higher grain yield under water-sav...Compared to drought-susceptible rice cultivars(DSRs),drought-resistance rice cultivars(DRRs)could drastically reduce the amount of irrigation water input and simultaneously result in higher grain yield under water-saving irrigation conditions.However,the mechanisms underlying these properties are unclear.We investigated how improved agronomic traits contribute to higher yield and higher water use efficiency(WUE)in DRRs than in DSRs under alternate wetting and drying(AWD).Two DRRs and two DSRs were field-grown in 2015 and 2016 using two different irrigation regimes:continuous flooding(CF)and AWD.Under CF,no statistical differences in grain yield and WUE were observed between DRRs and DSRs.Irrigation water under the AWD regime was 275–349 mm,an amount 49.8%–56.2% of that(552–620 mm)applied under the CF regime.Compared to CF,AWD significantly decreased grain yield in both DRRs and DSRs,with a more significant reduction in DSRs,and WUE was increased in DRRs,but not in DSRs,by 9.9%–23.0% under AWD.Under AWD,DRRs showed a 20.2%–26.2% increase in grain yield and an 18.6%–24.5% increase in WUE compared to DSRs.Compared to DSRs,DRRs showed less redundant vegetative growth,greater sink capacity,higher grain filling efficiency,larger root biomass,and deeper root distribution under AWD.We conclude that these improved agronomic traits exert positive influences on WUE in DRRs under AWD.展开更多
In the process of membrane absorption,spontaneous wetting of hydrophobic microporous membrane causes membrane modification and increases membrane phase mass transfer resistance,which have attracted wide interest.Howev...In the process of membrane absorption,spontaneous wetting of hydrophobic microporous membrane causes membrane modification and increases membrane phase mass transfer resistance,which have attracted wide interest.However,due to the limitations of previous testing methods,the study of the spontaneous membrane wetting process is limited.Herein,we present a method for monitoring spontaneous membrane wetting by measuring its alternating current(AC)impedance.The impedance tests of the PVDF flat membranes and hollow fiber membranes were conducted in a two-electrode system.The results of equivalent circuit fitting indicate that the impedance value of the unwetted membrane is about 1.02×10^(10)Ω,which is close to the theoretical value of 1.4×10^(10)Ω,and this method can quantify the electrochemical impedance value of membranes with different degrees of spontaneous wetting.In addition,a method of impedance test for real-time monitoring of spontaneous wetting was designed.During the experiment,the timeliness and continuity of this method are confirmed with exact judgment under different conditions.In future work,the impedance data will be used to build model to predict the percentage of membrane wetting degree.展开更多
In nature selenate reduction and nitrate denitrification both folow a similar biogeochemical mechanism. It has been proved that abiotic stresses such as alternative drying and wetting can exert an important influence ...In nature selenate reduction and nitrate denitrification both folow a similar biogeochemical mechanism. It has been proved that abiotic stresses such as alternative drying and wetting can exert an important influence on nitrate denitrification as well as on selenate reduction. Our experimental results lend great support to the above conclusion.展开更多
水分和氮素对水稻叶片光合特性和氮素吸收利用有重要影响,但在干湿交替灌溉条件下,水、氮是如何影响水稻叶片和根系氮代谢酶活性、产量和氮素吸收利用的仍不清楚。探明这一问题对于协同提高产量和氮肥利用效率有重要意义。本研究以超级...水分和氮素对水稻叶片光合特性和氮素吸收利用有重要影响,但在干湿交替灌溉条件下,水、氮是如何影响水稻叶片和根系氮代谢酶活性、产量和氮素吸收利用的仍不清楚。探明这一问题对于协同提高产量和氮肥利用效率有重要意义。本研究以超级稻品种南粳9108为材料,大田种植,设置全生育期常规灌溉(conventional irrigation,CI)和干湿交替灌溉(alternate wetting and drying irrigation,AWD)2种灌溉方式及5个施氮水平,不施氮(N0)、施氮90 kg hm^(-2)(N1)、施氮180 kg hm^(-2)(N2)、施氮270 kg hm^(-2)(N3)和施氮360 kg hm^(-2)(N4)。结果表明,与CI相比,AWD增加了水稻主要生育时期叶片的叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量,提高了叶片净光合速率,并显著增加了叶片中超氧化物歧化酶、过氧化氢酶、硝酸还原酶、谷氨酰胺合成酶和谷氨酸合成酶活性,显著降低了过氧化物酶、内肽酶活性和丙二醛含量,显著提高了根系中氮代谢酶硝酸还原酶、谷氨酰胺合成酶、谷氨酸合成酶和谷氨酸脱氢酶活性;AWD的产量较CI平均增加了10.4%。AWD显著提高了氮素转运量、氮素转运率、氮肥吸收利用率和氮肥偏生产力,产量和氮肥利用率均以AWD+N3处理组合的最高。因此,轻度干湿交替灌溉配合一定的施氮量,可以充分发挥水、肥效应,促进根系和叶片的氮代谢水平,提高叶片光合特性,协调地下地上部生长,有利于水稻产量和氮肥利用率的协同提高。展开更多
旨在探讨不同灌溉模式对籼粳杂交稻甬优1540产量与水分利用效率的影响,并阐明其相关生理基础。本研究以甬优1540为材料,设置了3种灌溉模式,即长淹灌溉(continuous flooding,CF)、轻度干湿交替灌溉(alternate wetting and moderate dryin...旨在探讨不同灌溉模式对籼粳杂交稻甬优1540产量与水分利用效率的影响,并阐明其相关生理基础。本研究以甬优1540为材料,设置了3种灌溉模式,即长淹灌溉(continuous flooding,CF)、轻度干湿交替灌溉(alternate wetting and moderate drying, AWMD)以及重度干湿交替灌溉(alternate wetting and severe drying, AWSD)。研究结果表明,与CF相比, AWMD与AWSD均能显著提高水分利用效率,增幅分别为22.6%~25.6%与18.2%~23.1%;AWMD可以显著提高水稻产量,增幅为8.6%~10.0%,而AWSD则显著降低水稻产量,降幅为6.0%~7.5%。与CF相比, AWMD显著降低了拔节期水稻的茎蘖数、地上部干物质重、叶面积指数、移栽至齐穗期的光合势以及移栽至拔节期的作物生长速率,但显著提高了茎蘖成穗率、拔节至齐穗期的作物生长速率、主要生育期水稻根长密度、深根比、比根长、根系总吸收表面积与活跃吸收表面积,以及灌浆后2次土壤复水期的剑叶净光合速率、根系氧化力、根系与叶片中玉米素和玉米素核苷(Z+ZR)含量、籽粒中蔗糖-淀粉代谢途径关键酶活性等指标。以上结果表明, AWMD可以协同提高甬优1540产量与水分利用效率,优化根-冠生长发育特征,提高灌浆期植株生理活性,实现高产与水分高效利用,为本研究最佳水分管理模式。展开更多
基金This work was supported by the National Natural Science Foundation of China(32071943,32272198).
文摘Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breeding and cultivation to improve nutritional quality.However,little information is available on differences in lysine anabolism between SG and IG and the underlying mechanism,and whether and how irrigation regimes affect lysine anabolism in these grains.A japonica rice cultivar was grown in the field and two irrigation regimes,continuous flooding(CF)and wetting alternating with partial drying(WAPD),were imposed from heading to the mature stage.Lysine content and activities of key enzymes of lysine biosynthesis,and levels of brassinosteroids(BRs)were lower in the IG than in the SG at the early grainfilling stage but higher at middle and late grain-filling stages.WAPD increased activities of these key enzymes,BR levels,and contents of lysine and total amino acids in IG,but not SG relative to CF.Application of 2,4-epibrassinolide to rice panicles in CF during early grain filling reproduced the effects of WAPD,but neither treatment altered the activities of enzymes responsible for lysine catabolism in either SG or IG.WAPD and elevated BR levels during grain filling increased lysine biosynthesis in IG.Improvement in lysine biosynthesis in rice should focus on IG.
基金supported by the National Natural Science Foundation of China (Grant No. 51769013)
文摘The typical loess on high slopes along the BaoLan High-speed Rail, China, was selected as the research object. The influence of the freeze-thaw cycle and dry-wet alternation on the shear-strength parameters of the unsaturated loess was investigated by laboratory experimental methods. Moreover, the temperature field, seepage field, and stability of slopes with different gradients were simulated under the effect of the freeze-thaw cycle and dry-wet alternation by using the geotechnical analysis software Geo-Studio. The research results showed(1) when the freeze-thaw cycle was repeated on the slope, with the frozen depth increasing, the melted depth did the same; besides, the closed loop of isotherms formed on the slope;(2) under the action of dry-wet circulation, the negative pore-water pressure and volumetric water content showed an upward tendency. However, owing to the different slope gradients, rainfall infiltration was not the same. As time went by, the differences of the negative pore-water pressure and volumetric water content between the slopes of different gradients continued to increase;(3) with the freeze-thaw cycle and dry-wet alternation increasing, the slope-safety factor decreased. Especially in the early period, the slope-safety factor changed remarkably. For slopes undergoing freeze-thaw action, the slope-safety factor was negatively correlated with the gradient. However, with regard to slopes undergoing dry-wet alternation, the result became more complex because the slope-safety factor was related to both seepage strength and slope grade. Accordingly, further research is needed to study the effect of seepage strength and slope grade on the stability of loess slopes.
基金AGER-Fondazioni in rete per la ricerca agroalimentare(https://www.progettoager.it/)(Grant No.2010-2369)by Joint Programming Initiative on Agriculture,Food Security and Climate Change(FACCE-JPI)project Green Rice(Sustainable and environmental friendly rice cultivation systems in Europe).
文摘Alternate wetting and drying(AWD)system,in which water has been reduced by approximately 35%with an increased occurrence of beneficial arbuscular mycorrhizal(AM)symbiosis and no negative impact on rice yield,was proposed to utilize water and nutrients more sustainable.In this study,we selected six rice cultivars(Centauro,Loto,Selenio,Vialone nano,JSendra and Puntal)grown under AWD conditions,and investigated their responsiveness to AM colonization and how they select diverse AM taxa.In order to investigate root-associated AM fungus communities,molecular cloning-Sanger sequencing on small subunit rDNA data were obtained from five out of the six rice cultivars and compared with Next Generation Sequencing(NGS)data,which were previously obtained in Vialone nano.The results showed that all the cultivars were responsive to AM colonization with the development of AM symbiotic structures,even if with differences in the colonization and arbuscule abundance in the root systems.We identified 16 virtual taxa(VT)in the soil compartment and 7 VT in the root apparatus.We emphasized that the NGS analysis gives additional value to the results thanks to a more in-depth reading of the less represented AM fungus taxa.
基金sponsored by the National Natural Science Foundation of China(31461143015,31271641,31471438)the National Key Technology Support Program of China(2014AA10A605,216YFD0300206-4)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),Chinathe Jiangsu Creation Program for Post-graduation Students,China(KYZZ15_0364)
文摘This study investigated if super rice could better cope with soil water deficit and if it could have better yield performance and water use efficiency (WUE) under alternate wetting and drying (AWD) irrigation than check rice. Two super rice cultivars and two elite check rice cultivars were grown in pots with three soil moisture levels, well watered (WW), moderate water deficit (MWD) and severe water deficit (SWD). Two cultivars, each for super rice and check rice, were grown in field with three irrigation regimes, alternate wetting and moderate drying (AWMD), alternate wetting and severe drying (AWSD) and conventional irrigation (CI). Compared with that under WW, grain yield was significantly decreased under MWD and SWD treatments, with less reduction for super rice than for check rice. Super rice had higher percentage of productive tillers, deeper root distribution, higher root oxidation activity, and greater aboveground biomass production at mid and late growth stages than check rice, especially under WMD and WSD. Compared with CI,AWMD increased, whereasAWSD decreased grain yield, with more increase or less decrease for super rice than for check rice. Both MWD and SWD treatments and eitherAWMD orAWSD regime significantly increased WUE compared with WW treatment or CI regime, with more increase for super rice than for check rice. The results suggest that super rice has a stronger ability to cope with soil water deficit and holds greater promising to increase both grain yield and WUE by adoption of moderate AWD irrigation.
文摘Direct seeded rice is promising alternative to traditional transplanting, but requires appropriate crop and water management to maintain yield performance and achieve high water productivity. Present study evaluated the effect of seed priming and irrigation on crop establishment, tillering, agronomic traits, paddy yield, grain quality and water productivity of direct seeded rice in alternate wetting and drying (DSR-AWD) in comparison with direct seeded rice at field capacity (DSR- FC). Seed priming treatments were osmo-priming with KCI (2.2%), CaCI2 (2.2%) and moringa leaf extracts (MLE, 3.3%) including hydro-priming as control. Among the treatments, seed osmo-primed with MLE emerged earlier and had higher final emergence, followed by osmo-priming with CaCI2. Tillering emergence rate and number of tillers per plant were the highest for seed priming with CaCI2 in DSR- AWD. Total productive and non-productive tillers, panicle length, biological and grain yields, harvest index were highest for seed priming with MLE or CaCI2 in DSR-AWD. Similarly, grain quality, estimated in terms of normal grains, abortive and chalky grains, was also the highest in DSR-AWD with MLE osmo-priming. Benefit cost ratio and water productivity was also the highest in DSR-AWD for seed priming with MLE. In conclusion, seed priming with MLE or CaCI2 can be successfully employed to improve the direct seeded rice performance when practiced with alternate wetting and drying irrigation.
基金the National Key Research and Development Program of China (2016YFD0300507,2016YFD0300108)the National Natural Science Foundation of China (31671630,31671638,31501264)the China Agriculture Research System (CARS-01)
文摘Compared to drought-susceptible rice cultivars(DSRs),drought-resistance rice cultivars(DRRs)could drastically reduce the amount of irrigation water input and simultaneously result in higher grain yield under water-saving irrigation conditions.However,the mechanisms underlying these properties are unclear.We investigated how improved agronomic traits contribute to higher yield and higher water use efficiency(WUE)in DRRs than in DSRs under alternate wetting and drying(AWD).Two DRRs and two DSRs were field-grown in 2015 and 2016 using two different irrigation regimes:continuous flooding(CF)and AWD.Under CF,no statistical differences in grain yield and WUE were observed between DRRs and DSRs.Irrigation water under the AWD regime was 275–349 mm,an amount 49.8%–56.2% of that(552–620 mm)applied under the CF regime.Compared to CF,AWD significantly decreased grain yield in both DRRs and DSRs,with a more significant reduction in DSRs,and WUE was increased in DRRs,but not in DSRs,by 9.9%–23.0% under AWD.Under AWD,DRRs showed a 20.2%–26.2% increase in grain yield and an 18.6%–24.5% increase in WUE compared to DSRs.Compared to DSRs,DRRs showed less redundant vegetative growth,greater sink capacity,higher grain filling efficiency,larger root biomass,and deeper root distribution under AWD.We conclude that these improved agronomic traits exert positive influences on WUE in DRRs under AWD.
基金Fanatical support from the National Natural Science Foundation of China(21576011)and the kind supply of electrochemical workstation from Prof。
文摘In the process of membrane absorption,spontaneous wetting of hydrophobic microporous membrane causes membrane modification and increases membrane phase mass transfer resistance,which have attracted wide interest.However,due to the limitations of previous testing methods,the study of the spontaneous membrane wetting process is limited.Herein,we present a method for monitoring spontaneous membrane wetting by measuring its alternating current(AC)impedance.The impedance tests of the PVDF flat membranes and hollow fiber membranes were conducted in a two-electrode system.The results of equivalent circuit fitting indicate that the impedance value of the unwetted membrane is about 1.02×10^(10)Ω,which is close to the theoretical value of 1.4×10^(10)Ω,and this method can quantify the electrochemical impedance value of membranes with different degrees of spontaneous wetting.In addition,a method of impedance test for real-time monitoring of spontaneous wetting was designed.During the experiment,the timeliness and continuity of this method are confirmed with exact judgment under different conditions.In future work,the impedance data will be used to build model to predict the percentage of membrane wetting degree.
文摘In nature selenate reduction and nitrate denitrification both folow a similar biogeochemical mechanism. It has been proved that abiotic stresses such as alternative drying and wetting can exert an important influence on nitrate denitrification as well as on selenate reduction. Our experimental results lend great support to the above conclusion.
文摘水分和氮素对水稻叶片光合特性和氮素吸收利用有重要影响,但在干湿交替灌溉条件下,水、氮是如何影响水稻叶片和根系氮代谢酶活性、产量和氮素吸收利用的仍不清楚。探明这一问题对于协同提高产量和氮肥利用效率有重要意义。本研究以超级稻品种南粳9108为材料,大田种植,设置全生育期常规灌溉(conventional irrigation,CI)和干湿交替灌溉(alternate wetting and drying irrigation,AWD)2种灌溉方式及5个施氮水平,不施氮(N0)、施氮90 kg hm^(-2)(N1)、施氮180 kg hm^(-2)(N2)、施氮270 kg hm^(-2)(N3)和施氮360 kg hm^(-2)(N4)。结果表明,与CI相比,AWD增加了水稻主要生育时期叶片的叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量,提高了叶片净光合速率,并显著增加了叶片中超氧化物歧化酶、过氧化氢酶、硝酸还原酶、谷氨酰胺合成酶和谷氨酸合成酶活性,显著降低了过氧化物酶、内肽酶活性和丙二醛含量,显著提高了根系中氮代谢酶硝酸还原酶、谷氨酰胺合成酶、谷氨酸合成酶和谷氨酸脱氢酶活性;AWD的产量较CI平均增加了10.4%。AWD显著提高了氮素转运量、氮素转运率、氮肥吸收利用率和氮肥偏生产力,产量和氮肥利用率均以AWD+N3处理组合的最高。因此,轻度干湿交替灌溉配合一定的施氮量,可以充分发挥水、肥效应,促进根系和叶片的氮代谢水平,提高叶片光合特性,协调地下地上部生长,有利于水稻产量和氮肥利用率的协同提高。
文摘旨在探讨不同灌溉模式对籼粳杂交稻甬优1540产量与水分利用效率的影响,并阐明其相关生理基础。本研究以甬优1540为材料,设置了3种灌溉模式,即长淹灌溉(continuous flooding,CF)、轻度干湿交替灌溉(alternate wetting and moderate drying, AWMD)以及重度干湿交替灌溉(alternate wetting and severe drying, AWSD)。研究结果表明,与CF相比, AWMD与AWSD均能显著提高水分利用效率,增幅分别为22.6%~25.6%与18.2%~23.1%;AWMD可以显著提高水稻产量,增幅为8.6%~10.0%,而AWSD则显著降低水稻产量,降幅为6.0%~7.5%。与CF相比, AWMD显著降低了拔节期水稻的茎蘖数、地上部干物质重、叶面积指数、移栽至齐穗期的光合势以及移栽至拔节期的作物生长速率,但显著提高了茎蘖成穗率、拔节至齐穗期的作物生长速率、主要生育期水稻根长密度、深根比、比根长、根系总吸收表面积与活跃吸收表面积,以及灌浆后2次土壤复水期的剑叶净光合速率、根系氧化力、根系与叶片中玉米素和玉米素核苷(Z+ZR)含量、籽粒中蔗糖-淀粉代谢途径关键酶活性等指标。以上结果表明, AWMD可以协同提高甬优1540产量与水分利用效率,优化根-冠生长发育特征,提高灌浆期植株生理活性,实现高产与水分高效利用,为本研究最佳水分管理模式。