Duck enteritis virus(DEV) is a herpesvirus that causes an acute,contagious and fatal disease. In the present article,the DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer ...Duck enteritis virus(DEV) is a herpesvirus that causes an acute,contagious and fatal disease. In the present article,the DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer for the consensus site of herpesvirus UL3 gene and a specific primer located in UL5 were used in the polymerase chain reaction(PCR) to amplify a DNA product 2 086 bp in size. DNA sequence analysis revealed that a 714 bp open reading frame(ORF) of DEV encoding a 237 amino acid polypeptide is homologous to the family of herpesvirus UL4 proteins and therefore has been characterized as a DEV UL4 gene. Alignment of the DEV UL4 protein sequence with those of other alphaherpesviruses showed that 10 amino acid residues are completely conserved. Phylogenetic tree analysis showed that the seventeen alphaherpesviruses viruses analyzed were classified into four large groups,and the duck enteritis virus branched separately,closely related to the Mardiviruses group comprising Gallid herpesvirus 2(GaHV-2) ,Gallid herpesvirus 3(GaHV-3) and Meleagrid herpesvirus 1(MeHV-1) . The present study showed that the evolutionary relationship of the UL4 protein could be used for classification of alphaherpesviruses.展开更多
The characteristics of duck virus enteritis(DVE)are introduced from the aspects of pathogen,epidemiology,clinical symptoms,autopsy pathological changes,etc.,and clinical comprehensive diagnosis and laboratory diagnosi...The characteristics of duck virus enteritis(DVE)are introduced from the aspects of pathogen,epidemiology,clinical symptoms,autopsy pathological changes,etc.,and clinical comprehensive diagnosis and laboratory diagnosis methods are given.The disease is distinguished from similar diseases including duck necrotic enteritis,duck candidiasis,duck hemorrhage,duck coccidiosis and duck vitamin A deficiency.Eventually,the prevention and treatment measures of the disease are put forward.展开更多
文摘Duck enteritis virus(DEV) is a herpesvirus that causes an acute,contagious and fatal disease. In the present article,the DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer for the consensus site of herpesvirus UL3 gene and a specific primer located in UL5 were used in the polymerase chain reaction(PCR) to amplify a DNA product 2 086 bp in size. DNA sequence analysis revealed that a 714 bp open reading frame(ORF) of DEV encoding a 237 amino acid polypeptide is homologous to the family of herpesvirus UL4 proteins and therefore has been characterized as a DEV UL4 gene. Alignment of the DEV UL4 protein sequence with those of other alphaherpesviruses showed that 10 amino acid residues are completely conserved. Phylogenetic tree analysis showed that the seventeen alphaherpesviruses viruses analyzed were classified into four large groups,and the duck enteritis virus branched separately,closely related to the Mardiviruses group comprising Gallid herpesvirus 2(GaHV-2) ,Gallid herpesvirus 3(GaHV-3) and Meleagrid herpesvirus 1(MeHV-1) . The present study showed that the evolutionary relationship of the UL4 protein could be used for classification of alphaherpesviruses.
文摘The characteristics of duck virus enteritis(DVE)are introduced from the aspects of pathogen,epidemiology,clinical symptoms,autopsy pathological changes,etc.,and clinical comprehensive diagnosis and laboratory diagnosis methods are given.The disease is distinguished from similar diseases including duck necrotic enteritis,duck candidiasis,duck hemorrhage,duck coccidiosis and duck vitamin A deficiency.Eventually,the prevention and treatment measures of the disease are put forward.
