[Objective] A mobility measurement and a regeneration assay were conducted to explore the behavioral effects of Cu2＋ on planarian Dugesia japonica. [Method] Dugesia japonica was respectively treated with different co...[Objective] A mobility measurement and a regeneration assay were conducted to explore the behavioral effects of Cu2＋ on planarian Dugesia japonica. [Method] Dugesia japonica was respectively treated with different concentrations of copper solution for 4 and 6 d, and the blank control group was set. The mobility measurement and a regeneration assay of copper ions were determined by mIC50 and rIC50 . [Result] The mobility IC 50 for adult D. japonica at 24, 48, 72 and 96 h were 86, 3.73, 3.47 and 2.61 mg Cu2＋ /L, respectively. The mobility IC50s for newborns was 2.22, 1.64, 0.87 and 0.93 mg Cu2＋ /L, respectively. Although there was no significant difference between 24 and 48 h, and between 72 and 96 h of copper exposure, Cu2＋ decreased newborns locomotor behavior in a concentration-dependent manner at sub-toxic concentrations. The regeneration IC50 of eyespots and auricles for adult D. japonica at 6 d were calculated as 0.76, 0.78 mg Cu2＋ /L, respectively. The number of eyespots and auricles regeneration in planarian D. japonica showed a concentration-dependent manner. Results showed that newborns mobility and adult regeneration assay for D. japonica in a concentration-dependent manner. [Conclusion] The results of the present study suggest that planarians can be regarded as a useful bioindicator species for toxicological studies in the future.展开更多
Freshwater planaria has tremendous capacity to reform the missing part of the body and therefore is considered as one of the most important model organism for regeneration study. At present, Schmidtea mediterranea and...Freshwater planaria has tremendous capacity to reform the missing part of the body and therefore is considered as one of the most important model organism for regeneration study. At present, Schmidtea mediterranea and Dugesia japonica are the two major species utilized for laboratory manipulations. Dugesia japonica flatworms are widely distributed in the Far East including Cherry Valley region in the north-west area of Beijing, China. We reported here the establishment of an asexual Dugesiajaponica strain Pek-1, as a suitable system for regeneration study. Using morphological, karyotypical as well as phylogenetic analyses, we confirmed that these flatworms indeed belonged to Dugesia japonica. We went on to show that the commonly used in situ probes and immunohistochemistry reagents and protocols were applicable to the Pek-1 strain. Using this strain, we carried out small scale analysis on EST, RNAi and gene expression. We identified 193 unique EST sequences and 65 of them had not been reported in planarian. By RNAi analysis, we showed that 48 genes, when down-regulated individually, had no effect on regeneration. Furthermore, we identified 3 groups of tissue specific expressing genes that were useful for cell lineage analysis. We concluded that the Dugesiajaponica Pek-1 strain could be another suitable animal model to regeneration research.展开更多
The freshwater planarian Dugesia japonica has become a model organism in regeneration biology and toxicology due to its powerful regeneration capability and highly chemical sensitivity.Nonetheless,little is known abou...The freshwater planarian Dugesia japonica has become a model organism in regeneration biology and toxicology due to its powerful regeneration capability and highly chemical sensitivity.Nonetheless,little is known about its evolutionary history and demographics.Taihang Mountains is the natural boundary between the Loess Plateau and the North China Plain,and is considered as one of the important priority areas for biodiversity conservation in China.In order to figure out the genetic diversity,population structure and demographic history of D.japonica in Taihang Mountains,a study based on the mitochondrial COI from 116 individuals sampled across 20 populations has been conducted.The results showed that the 116 COI sequences yielded 32 haplotypes,including 8 shared haplotypes and 24 private ones.The overall haplotype diversity(Hd)and nucleotide diversity(π)were 0.920 and 0.083,respectively.Even though the AMOVA results suggested that the genetic variation among populations was significant(FST=0.480,P<0.01),the phylogeny and haplotype network analysis based on 32 haplotypes revealed no obvious phylogeographic pattern.Furthermore,the significantly positive values of neutrality test(Tajima’s D=2.596,P<0.05;Fu’s Fs=2.769,P<0.01)together with the multimodal arrangement of mismatch distribution indicated that D.japonica in Taihang Mountains would have been undergoing population decline.We hope these findings will arouse conservation and management strategy regarding freshwater planarians and contribute to the biodiversity in the long run.展开更多
基金Supported by Natural Science Foundation of Shandong Province(ZR2009BM017,ZR2009DM029)National Natural Science Foundation of China(31172074)~~
文摘[Objective] A mobility measurement and a regeneration assay were conducted to explore the behavioral effects of Cu2＋ on planarian Dugesia japonica. [Method] Dugesia japonica was respectively treated with different concentrations of copper solution for 4 and 6 d, and the blank control group was set. The mobility measurement and a regeneration assay of copper ions were determined by mIC50 and rIC50 . [Result] The mobility IC 50 for adult D. japonica at 24, 48, 72 and 96 h were 86, 3.73, 3.47 and 2.61 mg Cu2＋ /L, respectively. The mobility IC50s for newborns was 2.22, 1.64, 0.87 and 0.93 mg Cu2＋ /L, respectively. Although there was no significant difference between 24 and 48 h, and between 72 and 96 h of copper exposure, Cu2＋ decreased newborns locomotor behavior in a concentration-dependent manner at sub-toxic concentrations. The regeneration IC50 of eyespots and auricles for adult D. japonica at 6 d were calculated as 0.76, 0.78 mg Cu2＋ /L, respectively. The number of eyespots and auricles regeneration in planarian D. japonica showed a concentration-dependent manner. Results showed that newborns mobility and adult regeneration assay for D. japonica in a concentration-dependent manner. [Conclusion] The results of the present study suggest that planarians can be regarded as a useful bioindicator species for toxicological studies in the future.
基金supported by grant from the National Natural Science Foundation of China to W.W. (No. 30670225)
文摘Freshwater planaria has tremendous capacity to reform the missing part of the body and therefore is considered as one of the most important model organism for regeneration study. At present, Schmidtea mediterranea and Dugesia japonica are the two major species utilized for laboratory manipulations. Dugesia japonica flatworms are widely distributed in the Far East including Cherry Valley region in the north-west area of Beijing, China. We reported here the establishment of an asexual Dugesiajaponica strain Pek-1, as a suitable system for regeneration study. Using morphological, karyotypical as well as phylogenetic analyses, we confirmed that these flatworms indeed belonged to Dugesia japonica. We went on to show that the commonly used in situ probes and immunohistochemistry reagents and protocols were applicable to the Pek-1 strain. Using this strain, we carried out small scale analysis on EST, RNAi and gene expression. We identified 193 unique EST sequences and 65 of them had not been reported in planarian. By RNAi analysis, we showed that 48 genes, when down-regulated individually, had no effect on regeneration. Furthermore, we identified 3 groups of tissue specific expressing genes that were useful for cell lineage analysis. We concluded that the Dugesiajaponica Pek-1 strain could be another suitable animal model to regeneration research.
基金supported by the National Natural Science Foundation of China(31702010)Major Public Welfare Project of Henan Province(201300311700)Puyang Field Scientific Observation and Research Station for Yellow River Wetland Ecosystem,Henan Province。
文摘The freshwater planarian Dugesia japonica has become a model organism in regeneration biology and toxicology due to its powerful regeneration capability and highly chemical sensitivity.Nonetheless,little is known about its evolutionary history and demographics.Taihang Mountains is the natural boundary between the Loess Plateau and the North China Plain,and is considered as one of the important priority areas for biodiversity conservation in China.In order to figure out the genetic diversity,population structure and demographic history of D.japonica in Taihang Mountains,a study based on the mitochondrial COI from 116 individuals sampled across 20 populations has been conducted.The results showed that the 116 COI sequences yielded 32 haplotypes,including 8 shared haplotypes and 24 private ones.The overall haplotype diversity(Hd)and nucleotide diversity(π)were 0.920 and 0.083,respectively.Even though the AMOVA results suggested that the genetic variation among populations was significant(FST=0.480,P<0.01),the phylogeny and haplotype network analysis based on 32 haplotypes revealed no obvious phylogeographic pattern.Furthermore,the significantly positive values of neutrality test(Tajima’s D=2.596,P<0.05;Fu’s Fs=2.769,P<0.01)together with the multimodal arrangement of mismatch distribution indicated that D.japonica in Taihang Mountains would have been undergoing population decline.We hope these findings will arouse conservation and management strategy regarding freshwater planarians and contribute to the biodiversity in the long run.
