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Characteristics, Genesis, and Sedimentary Environment of Duplex-Like Structures in the Jurassic Sediments of Western Qaidam Basin, China 被引量:3
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作者 Liangtian Ni Jianhua Zhong +3 位作者 Zhufu Shao Yong Li Cui Mao Shengxin Liu 《Journal of Earth Science》 SCIE CAS CSCD 2015年第5期677-689,共13页
A particular type of soft-sediment deformation structure, similar to imbricate structure, is developed in the Jurassic strata at Honggouzi, western Qaidam Basin, China. We refer to this structure as a duplex-like defo... A particular type of soft-sediment deformation structure, similar to imbricate structure, is developed in the Jurassic strata at Honggouzi, western Qaidam Basin, China. We refer to this structure as a duplex-like deformation structure, because it is inferred to have been formed by the action of fast-moving, submarine gravity current sediments gliding across a pre-existing semiconsolidated sedimentary layer. The layers of duplex-like structure crop out in the southeastern limb of the core of a medium-sized anticline. The average dip direction of the duplex-like structure is 301.2° and the average dip angle is 54.7°. Duplex-like deformed laminations are composed mainly of weakly metamorphosed, extremely poorly sorted, feldspathic lithic graywacke. Sericite can be observed along bedding planes. The duplex-like structure occurs within a sequence of river-channel fine conglomerate, interchannel carbonaceous mudstone(shale), shallow-water delta sand-shale, shallow-lake calcareous mudstone, olistostromes, and a turbidite. At the bottom of the sequence, we found brownish-red shallow-lake calcareous mudstone and carbonaceous mudstone and at the top olistostromes whose genesis is related to the slip and drag of a slumped body of submarine sediment. In combination with other symbiotic and associated structures, it is considered that the duplex-like structure was formed by the slumping and subsequent movement and traction of delta-front semiconsolidated sediments over the bottom sediments of a shore or shallow-lake sedimentary environment, probably triggered by an earthquake. The attitude of the duplex-like structure indicates that the direction of gliding was from NW301.2° toward SE121°, which is consistent with the current location of the Altun Mountains, indicating that the Altun Mountains existed in the Jurassic. The orogenesis of these mountains likely involved seismic activity, and an earthquake was the triggering factor in the formation of the duplex-like structure by causing the slumping/gliding of the olistostrome sediments. 展开更多
关键词 duplex-like structure soft-sediment deformation turbidity current slumping and glid-ing western Qaidam Basin
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类NADC30与类NADC34PRRSV双重qPCR检测方法的建立与应用
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作者 马明洋 余文轩 +7 位作者 安川叶 张晶晶 杨紫烨 殷笑妍 杜叶 朱瑞冰 韩冬梅 刘冠慧 《中国动物检疫》 CAS 2024年第12期89-94,共6页
近年来,类NADC3O和类NADC34猪繁殖与呼吸综合征病毒(PRRSV)给养猪业带来了严重经济损失。为实现类NADC30和类NADC34 PRRSV的同时检测,针对PRRSV经典毒株、高致病性毒株、类NADC30毒株和类NADC34毒株ORF5基因的明显差异区域序列,设计引... 近年来,类NADC3O和类NADC34猪繁殖与呼吸综合征病毒(PRRSV)给养猪业带来了严重经济损失。为实现类NADC30和类NADC34 PRRSV的同时检测,针对PRRSV经典毒株、高致病性毒株、类NADC30毒株和类NADC34毒株ORF5基因的明显差异区域序列,设计引物与探针,经优化引物和探针浓度及退火温度,建立了类NADC30和类NADC34 PRRSV双重qPCR检测方法,并对该方法的特异性、敏感性和重复性进行评估,比较该方法与文献报道方法对临床样本检测结果的一致性。结果显示:本研究建立的双重qPCR方法仅对类NADC30和类NADC34 PRRSV核酸扩增呈阳性,而对其他常见猪病病原均无扩增,且类NADC30与类NADC34 PRRSV检测方法之间无交叉反应;对类NADC30和类NADC34PRRSV核酸的最低检测限分别为1和10 copies/uL,组内及组间试验变异系数均小于2%;该方法与文献报道方法对类NADC30和类NADC34 PRRSV检测结果的符合率分别为89.5%和92.9%。结果表明,本研究建立的双重qPCR方法特异性好、灵敏度高、稳定性佳,能够有效区分类NADC30和类NADC34 PRRSV,为猪繁殖与呼吸综合征诊断和流行病学调查提供了技术支撑。 展开更多
关键词 类NADC30 PRRSV 类NADC34 PRRSV 双重qPCR 建立及应用
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布鲁菌二联BCSP31-L7/L12-IL-2嵌合病毒样颗粒疫苗候选株的构建与鉴定 被引量:1
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作者 邹映雪 黄天鸿 +6 位作者 冯嘉轩 丁佳欣 陈铭桦 陈凯楠 郭春红 张頔 丁壮 《中国兽医学报》 CAS CSCD 北大核心 2023年第5期865-871,共7页
布鲁菌病的传统疫苗存在接种后干扰血清学诊断及可能出现毒力返强等缺点,严重干扰疫病监测,影响疫病净化。因此,本研究基于新城疫病毒样颗粒(ND virus like particles,ND VLPs)载体平台,利用昆虫杆状病毒表达系统和GPI锚定策略,以免疫... 布鲁菌病的传统疫苗存在接种后干扰血清学诊断及可能出现毒力返强等缺点,严重干扰疫病监测,影响疫病净化。因此,本研究基于新城疫病毒样颗粒(ND virus like particles,ND VLPs)载体平台,利用昆虫杆状病毒表达系统和GPI锚定策略,以免疫增强因子IL-2、高保守性的保护性抗原蛋白L7/L12(来源猪种S2株)和羊种BCSP31蛋白(来源羊种M5株)为靶点,构建绿色、安全的布鲁菌二联嵌合型病毒样颗粒新型疫苗候选株(BCSP31-L7/L12-IL-2 cVLPs)。PCR及免疫荧光结果表明成功构建了表达IL-2和L7/L12蛋白的重组杆状病毒,Western blot结果显示该cVLPs各组分蛋白正确表达,透射电镜观察可见表面展示纤突结构、大小约为100 nm的粒子,表明布鲁菌二联嵌合型病毒样颗粒BCSP31-L7/L12-IL-2 cVLPs构建成功。 展开更多
关键词 布鲁菌 新城疫病毒样颗粒载体 二联嵌合型病毒样颗粒疫苗 L7/L12蛋白 BCSP31蛋白
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