The development of super-resolution technology has made it possible to investigate the ultrastructure of intracellular organelles by fuorescence microscopy,which has greatly facilitated the development of life science...The development of super-resolution technology has made it possible to investigate the ultrastructure of intracellular organelles by fuorescence microscopy,which has greatly facilitated the development of life sciences and biomedicine.To realize super-resolution imaging of living cells,both advanced imaging systems and excellent fuorescent probes are required.Traditional fuorescent probes have good availability,but that is not the case for probes for live-cell super-resolution imaging.In this review,we frst introduce the principles of various super-resolution technologies and their probe requirements,then summarize the existing designs and delivery strategies of super-resolution probes for live-cell imaging,and fnally provide a brief conclusion and overview of the future.展开更多
基金supported by the National Key Research and Development Program of China(No.2022YFC3401100)the National Natural Science Foundation of China(Grant Nos.32271428,92054110,and 32201132)China Postdoctoral Science Foundation funded project(Nos.BX20220125 and 2022M711257).
文摘The development of super-resolution technology has made it possible to investigate the ultrastructure of intracellular organelles by fuorescence microscopy,which has greatly facilitated the development of life sciences and biomedicine.To realize super-resolution imaging of living cells,both advanced imaging systems and excellent fuorescent probes are required.Traditional fuorescent probes have good availability,but that is not the case for probes for live-cell super-resolution imaging.In this review,we frst introduce the principles of various super-resolution technologies and their probe requirements,then summarize the existing designs and delivery strategies of super-resolution probes for live-cell imaging,and fnally provide a brief conclusion and overview of the future.
