New approach to assess sperm DNA fragmentation dynamics： Fine-tuning mathematical models

Background： Sperm DNA fragmentation（sDF） has been proved to be an important parameter in order to predict in vitro the potential fertility of a semen sample. Colloid centrifugation could be a suitable technique to select those donkey sperm more resistant to DNA fragmentation after thawing. Previous studies have shown that to elucidate the latent damage of the DNA molecule, sDF should be assessed dynamically, where the rate of fragmentation between treatments indicates how resistant the DNA is to iatrogenic damage. The rate of fragmentation is calculated using the slope of a linear regression equation. However, it has not been studied if s DF dynamics fit this model. The objectives of this study were to evaluate the effect of different after-thawing centrifugation protocols on sperm DNA fragmentation and elucidate the most accurate mathematical model（linear regression, exponential or polynomial） for DNA fragmentation over time in frozen-thawed donkey semen.Results： After submitting post-thaw semen samples to no centrifugation（UDC）, sperm washing（SW） or single layer centrifugation（SLC） protocols, sD F values after 6 h of incubation were significantly lower in SLC samples than in SW or UDC.Coefficient of determination（R-2） values were significantly higher for a second order polynomial model than for linear or exponential. The highest values for acceleration of fragmentation（aSDF） were obtained for SW, fol owed by SLC and UDC.Conclusion： SLC after thawing seems to preserve longer DNA longevity in comparison to UDC and SW. Moreover,the fine-tuning of models has shown that sDF dynamics in frozen-thawed donkey semen fit a second order polynomial model, which implies that fragmentation rate is not constant and fragmentation acceleration must be taken into account to elucidate hidden damage in the DNA molecule.

A Comparative Study of the Seismic Performances and Failure Mechanisms of Slopes Using Dynamic Centrifuge Modeling

In this study, dynamic centrifuge model tests were performed for sand slopes under different earthquake ground motions and slope angle to characterize the seismic performance of slopes. Four groups of tests under varying seismic input amplitude were conducted. Under the action of increasing earthquake intensity, the rigidity of the soil decreases and the damping ratio increases, both of the dynamic response and the predominant period of slopes are increased. Three types of seismic waves with the same seismic intensity were applied in the model tests. It shows that the variability in the ground motion leads to the acceleration response spectra of the slopes being completely different and the Northridge seismic wave with low-frequency component is closest to the predominant period of the slope model. In addition, the effect of slope angle on the seismic performance of slopes were also clarified. The results reveal how the slope angle affects the acceleration recorded on the ground surface of the slope, both in terms of the peak ground-motion acceleration(PGA) amplification factor and the predominant period. Finally, the permanent displacement of the model slopes under different earthquake intensities were further analyzed. Based on the nonlinear growth of the permanent displacement of the slope, the test results demonstrated the failure process of the slope, which can further provide a basis for theperformance-based seismic design of slopes.