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Dynamic Pathology and Antigen Location Study on Broiler Breeders with Coinfection of Marek's Disease Virus and Reticuloendotheliosis Virus 被引量:1
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作者 DIAO Xiu-guo ZHU Guo CHENG Zi-qiang WANG Gui-hua MENG Xiang-kai GAO Ting-ting CUI Zhi-zhong 《Agricultural Sciences in China》 CAS CSCD 2008年第11期1387-1393,共7页
To further understand the generation and development of coinfection of Marek's disease virus (MDV) and reticuloendotheliosis virus (REV) in broiler breeders, and then find the method and optimal time of different... To further understand the generation and development of coinfection of Marek's disease virus (MDV) and reticuloendotheliosis virus (REV) in broiler breeders, and then find the method and optimal time of differential diagnosis for complex clinic multiple infection, the authors studied the pathohistological changes, apoptosis, immunohistochemistry (immunofluorescence), and ultrastructure of tumor tissues of broiler breeders inoculated with MDV and REV. The study showed that proliferation of small lymphocytes was seen in the main organs at the age of 1 week, then immature lymphocytes, all kinds of lymphocytes, primitive reticulum cells, and Marek's disease cells (MDCs) were observed at 2-9 weeks. Apoptosis of lymphocytes could not be seen until the age of 10 weeks in the immune system. Immunohistochemistry detection showed that the positive signs of MDV and REV antigen were observed in the main organs at 2 weeks of age. Multi-morphology lymphocytes, MDV, and REV, mitotic figures and apoptosis of lymphocytes were observed with the help of transmission electron microscopy. MDV cooperating with REV promotes the course of disease of coinfection. Differential diagnosis can be done by immunohistochemistry in the early stage (before 2 weeks), and histopathology in the late stage (post 4 weeks). MDCs, primitive reticulum cells, immature lymphocytes, and two kinds of virions can serve as a basis for bistopathology differential diagnosis. 展开更多
关键词 Marek's disease virus reticuloendotheliosis virus COINFECTION dynamic pathology antigen location
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