期刊文献+
共找到36,811篇文章
< 1 2 250 >
每页显示 20 50 100
A Study on Framework of Open Standard and Intellectual Property in E-Business
1
作者 Bi Chunli Shu Huaying Xi Jiarong 《China Communications》 SCIE CSCD 2007年第4期89-93,共5页
With the progress and development of information society,electronic business(e-Business) becomes a new model of business.E-Business does lower the cost of trading significantly;furthermore it allows restructuring the ... With the progress and development of information society,electronic business(e-Business) becomes a new model of business.E-Business does lower the cost of trading significantly;furthermore it allows restructuring the supply chain,business processes, and the nature of business.Open standard plays an important role in expanding e-Business,ranging from the Internet protocol(TCP/IP,HTTP...) to nearly all aspects of information technology that related to e-Business.More importantly,openness has emerged as a strategy with many of the most successful e-Business ventures granting business partner and consumers unparallel access to their inner working and personnel.Open standards have given rise to a prodigious international expansion in the number of users and range of applications relevant to daily life.The traditional market failure argument suggests that because innovation is expensive to produce and cheap to copy,so more intellectual property incorporated into the standards,and e-Business is inevitable.This paper defines the new framework of e-Business,which describes the relationship of the public benefit from Open Standard and the private benefit from intellectual property and try to find a balance between the benefits of the patent holders,industry,and end-users in order to realize a winning situation for all parties. 展开更多
关键词 IPR e-business OPEN STANDARD
下载PDF
Construction of 3D porous Cu_(1.81)S/nitrogen-doped carbon frameworks for ultrafast and long-cycle life sodium-ion storage
2
作者 Chen Chen Hongyu Xue +6 位作者 Qilin Hu Mengfan Wang Pan Shang Ziyan Liu Tao Peng Deyang Zhang Yongsong Luo 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS 2025年第1期191-200,共10页
Transition metal sulfides have great potential as anode mterials for sodium-ion batteries(SIBs)due to their high theoretical specific capacities.However,the inferior intrinsic conductivity and large volume variation d... Transition metal sulfides have great potential as anode mterials for sodium-ion batteries(SIBs)due to their high theoretical specific capacities.However,the inferior intrinsic conductivity and large volume variation during sodiation-desodiation processes seriously affect its high-rate and long-cyde performance,unbeneficial for the application as fast-charging and long-cycling SIBs anode.Herein,the three-dimensional porous Cu_(1.81)S/nitrogen-doped carbon frameworks(Cu_(1.81)S/NC)are synthesized by the simple and facile sol-gel and annealing processes,which can accommodate the volumetric expansion of Cu_(1.81)S nanoparticles and accelerate the transmission of ions and electrons during Na^(+)insertion/extraction processes,exhibiting the excellent rate capability(250.6 mA·g^(-1)at 20.0 A·g^(-1))and outstanding cycling stability(70% capacity retention for 6000 cycles at 10.0 A·g^(-1))for SIBs.Moreover,the Na-ion full cells coupled with Na_(3)V_(2)(PO_(4))_(3)/C cathode also demonstrate the satisfactory reversible specific capacity of 330.5 mAh·g^(-1)at 5.0 A·g^(-1)and long-cycle performance with the 86.9% capacity retention at 2.0 A·g^(-1)after 750 cycles.This work proposes a promising way for the conversionbased metal sulfides for the applications as fast-charging sodium-ion battery anode. 展开更多
关键词 copper sulfide nanoparticles porous carbon framework fast charging long-cycle performance sodium-ion full batteries
下载PDF
E-business在企业价值创造过程中的评价模型
3
作者 张传波 《科技和产业》 2005年第6期36-38,共3页
电子商务作为一种崭新的商业模式,它的发展必将有利地推动传统企业经济效益的提高,对我国经济的发展产生重要的影响作用。本文从系统论与突变论的角度出发,在分析企业经济效益模型的理论基础上建立了数学模型,详细探讨了致使企业经济效... 电子商务作为一种崭新的商业模式,它的发展必将有利地推动传统企业经济效益的提高,对我国经济的发展产生重要的影响作用。本文从系统论与突变论的角度出发,在分析企业经济效益模型的理论基础上建立了数学模型,详细探讨了致使企业经济效益发生质变的临界点,并阐述了其作用效果与途径。 展开更多
关键词 e-business 价值创造 评价模型
下载PDF
传统批发业走进e-business时代的策略思考 被引量:1
4
作者 赵秋梅 《经济师》 北大核心 2001年第1期22-22,共1页
关键词 商业 e-business时代 批发业 电子商务 信息化
下载PDF
浅谈企业实施E-Business系统的关键成功要素CSFs及成功标准 被引量:1
5
作者 张璐璐 《江西财经大学学报》 2003年第1期62-65,共4页
本文透视了E-Business的发展现状,建立了关系模型来揭示E-Business四个主要组成部分(ERP,SCM,E-Commerce和CRM)之间的关系以及它们如何为不同的战略目标服务,在大量的实际调查和现有案例研究结果的基础上,总结了E-Business系统实施的关... 本文透视了E-Business的发展现状,建立了关系模型来揭示E-Business四个主要组成部分(ERP,SCM,E-Commerce和CRM)之间的关系以及它们如何为不同的战略目标服务,在大量的实际调查和现有案例研究结果的基础上,总结了E-Business系统实施的关键成功要素CSFs,并分析其对系统实施的影响,比较了ERP,SCM,E-Commerce和CRM实施的不同成功标准,旨在为企业组织实施工作和评估系统实施效果提供可靠的依据。 展开更多
关键词 企业 e-business系统 CSFs 成功标准 企业资源计划 ERP 供应链管理 SCM 客户关系管理 企业资源管理 ERM
下载PDF
Identifying E-Business Model: A Value Chain-Based Analysis 被引量:1
6
作者 ZENG Qingfeng, HUANG Lihua (Department of Information Management and Information Systems, School of Management, Fudan University Shanghai 200433 China) 《Journal of Electronic Science and Technology of China》 2004年第3期146-151,共6页
E-business will change the ways that all companies do business, and most traditional businesses will evolve from their current business model to a combination of place and space via e-business model. To choose the pro... E-business will change the ways that all companies do business, and most traditional businesses will evolve from their current business model to a combination of place and space via e-business model. To choose the proper e-business model becomes the important strategic concern for company to succeed. The main objective of this paper is to investigate the analysis framework for identifying e-business model. Based on the e-business process, from the value chain to the value net perspective. This paper provides a theoretical framework for identifying e-business models, and results in 11 e-business models. The strategic intend of every e-business model is discussed in the end of this paper. An enterprise e-business model design and implementation can be specified by the combination of one or more among 11 e-business models. 展开更多
关键词 e-business mode value chain e-business strategy
下载PDF
Challenges and Opportunities in Preserving Key Structural Features of 3D-Printed Metal/Covalent Organic Framework 被引量:1
7
作者 Ximeng Liu Dan Zhao John Wang 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第8期362-381,共20页
Metal-organic framework(MOF)and covalent organic framework(COF)are a huge group of advanced porous materials exhibiting attractive and tunable microstructural features,such as large surface area,tunable pore size,and ... Metal-organic framework(MOF)and covalent organic framework(COF)are a huge group of advanced porous materials exhibiting attractive and tunable microstructural features,such as large surface area,tunable pore size,and functional surfaces,which have significant values in various application areas.The emerging 3D printing technology further provides MOF and COFs(M/COFs)with higher designability of their macrostructure and demonstrates large achievements in their performance by shaping them into advanced 3D monoliths.However,the currently available 3D printing M/COFs strategy faces a major challenge of severe destruction of M/COFs’microstructural features,both during and after 3D printing.It is envisioned that preserving the microstructure of M/COFs in the 3D-printed monolith will bring a great improvement to the related applications.In this overview,the 3D-printed M/COFs are categorized into M/COF-mixed monoliths and M/COF-covered monoliths.Their differences in the properties,applications,and current research states are discussed.The up-to-date advancements in paste/scaffold composition and printing/covering methods to preserve the superior M/COF microstructure during 3D printing are further discussed for the two types of 3D-printed M/COF.Throughout the analysis of the current states of 3D-printed M/COFs,the expected future research direction to achieve a highly preserved microstructure in the 3D monolith is proposed. 展开更多
关键词 Metal-organic frameworks Covalent organic frameworks 3D printing Microstructure MONOLITH
下载PDF
ICT Workers and Professional Spirit in the E-Business Era 被引量:1
8
作者 MURATA Kiyoshi HIGASHIMOTO Shigeki 《Journal of Electronic Science and Technology of China》 2004年第3期104-109,共6页
Because of the ubiquity of information and communication technology (ICT) and ICT-based information systems, ICT workers in business organisations have power, both intentional and unintentional, over people's qual... Because of the ubiquity of information and communication technology (ICT) and ICT-based information systems, ICT workers in business organisations have power, both intentional and unintentional, over people's quality of life. On this point, all of them, not only ICT professionals but also non-professionals or end-users, need to accept responsibility to the public and to have professional spirit in order to make and keep the information society and e-business environment safe and reliable. However, an individual ICT worker is not necessarily a strong existence; he or she is in a complex situation filled with stress and pressure and his or her decisions are sometimes affected by it. Therefore, social and organisational measures to prompt ICT workers to behave in professional spirit are necessary to be taken. 展开更多
关键词 ICT worker professional spirit e-business environment moral working environment
下载PDF
An Exploratory Study of E-Business Success Factors 被引量:1
9
作者 LI Jingting, HUANG Jinghua (School of Economic Management, Tsinghua University Beijing 100084 China) 《Journal of Electronic Science and Technology of China》 2004年第3期167-172,共6页
E-business success factors are Important for traditional enterprises to implement e-business. This topic is attracting more and more researchers to study. This paper makes an exploratory study on the factors influenci... E-business success factors are Important for traditional enterprises to implement e-business. This topic is attracting more and more researchers to study. This paper makes an exploratory study on the factors influencing e-business success. Firstly, based on the literature review, 52 factors are suggested. Secondly, two rounds of survey with Delphi method are conducted. Qualitative and quantitative analysis are used to identify 57 factors. This is the foundation of empirical study. 展开更多
关键词 e-business success factors comprehensive factors web features and functions
下载PDF
Parallel Light Fields: A Perspective and A Framework 被引量:1
10
作者 Fei-Yue Wang Yu Shen 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2024年第2期542-544,共3页
Dear Editor,Light fields give relatively complete description of scenes from perspective of angles and positions of rays. At present time, most of the computer vision algorithms take 2D images as input which are simpl... Dear Editor,Light fields give relatively complete description of scenes from perspective of angles and positions of rays. At present time, most of the computer vision algorithms take 2D images as input which are simplified expression of light fields with depth information discarded. In theory, computer vision tasks may achieve better performance as long as complete light fields are acquired. 展开更多
关键词 COMPUTER framework simplified
下载PDF
Porous framework materials for energy&environment relevant applications:A systematic review 被引量:2
11
作者 Yutao Liu Liyu Chen +16 位作者 Lifeng Yang Tianhao Lan Hui Wang Chenghong Hu Xue Han Qixing Liu Jianfa Chen Zeming Feng Xili Cui Qianrong Fang Hailong Wang Libo Li Yingwei Li Huabin Xing Sihai Yang Dan Zhao Jinping Li 《Green Energy & Environment》 SCIE EI CAS CSCD 2024年第2期217-310,共94页
Carbon peaking and carbon neutralization trigger a technical revolution in energy&environment related fields.Development of new technologies for green energy production and storage,industrial energy saving and eff... Carbon peaking and carbon neutralization trigger a technical revolution in energy&environment related fields.Development of new technologies for green energy production and storage,industrial energy saving and efficiency reinforcement,carbon capture,and pollutant gas treatment is in highly imperious demand.The emerging porous framework materials such as metal–organic frameworks(MOFs),covalent organic frameworks(COFs)and hydrogen-bonded organic frameworks(HOFs),owing to the permanent porosity,tremendous specific surface area,designable structure and customizable functionality,have shown great potential in major energy-consuming industrial processes,including sustainable energy gas catalytic conversion,energy-efficient industrial gas separation and storage.Herein,this manuscript presents a systematic review of porous framework materials for global and comprehensive energy&environment related applications,from a macroscopic and application perspective. 展开更多
关键词 Porous framework materials CATALYSIS SEPARATION Gas storage Carbon neutrality
下载PDF
All‑Covalent Organic Framework Nanofilms Assembled Lithium‑Ion Capacitor to Solve the Imbalanced Charge Storage Kinetics 被引量:2
12
作者 Xiaoyang Xu Jia Zhang +6 位作者 Zihao Zhang Guandan Lu Wei Cao Ning Wang Yunmeng Xia Qingliang Feng Shanlin Qiao 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第6期246-260,共15页
Free-standing covalent organic framework(COFs)nanofilms exhibit a remarkable ability to rapidly intercalate/de-intercalate Li^(+) in lithium-ion batteries,while simultaneously exposing affluent active sites in superca... Free-standing covalent organic framework(COFs)nanofilms exhibit a remarkable ability to rapidly intercalate/de-intercalate Li^(+) in lithium-ion batteries,while simultaneously exposing affluent active sites in supercapacitors.The development of these nanofilms offers a promising solution to address the persistent challenge of imbalanced charge storage kinetics between battery-type anode and capacitor-type cathode in lithium-ion capacitors(LICs).Herein,for the first time,custom-made COFBTMB-TP and COFTAPB-BPY nanofilms are synthesized as the anode and cathode,respectively,for an all-COF nanofilm-structured LIC.The COFBTMB-TP nanofilm with strong electronegative–CF3 groups enables tuning the partial electron cloud density for Li^(+) migration to ensure the rapid anode kinetic process.The thickness-regulated cathodic COFTAPB-BPY nanofilm can fit the anodic COF nanofilm in the capacity.Due to the aligned 1D channel,2D aromatic skeleton and accessible active sites of COF nanofilms,the whole COFTAPB-BPY//COFBTMB-TP LIC demonstrates a high energy density of 318 mWh cm^(−3) at a high-power density of 6 W cm^(−3),excellent rate capability,good cycle stability with the capacity retention rate of 77%after 5000-cycle.The COFTAPB-BPY//COFBTMB-TP LIC represents a new benchmark for currently reported film-type LICs and even film-type supercapacitors.After being comprehensively explored via ex situ XPS,7Li solid-state NMR analyses,and DFT calculation,it is found that the COFBTMB-TP nanofilm facilitates the reversible conversion of semi-ionic to ionic C–F bonds during lithium storage.COFBTMB-TP exhibits a strong interaction with Li^(+) due to the C–F,C=O,and C–N bonds,facilitating Li^(+) desolation and absorption from the electrolyte.This work addresses the challenge of imbalanced charge storage kinetics and capacity between the anode and cathode and also pave the way for future miniaturized and wearable LIC devices. 展开更多
关键词 Covalent organic frameworks Lithium-ion capacitor Charge storage kinetic
下载PDF
浅论ERP和E-business的有效结合
13
作者 王强 《经济技术协作信息》 2006年第16期7-7,共1页
一、企业的ERP与E-business 在我国,企业实施“企业资源计划(ERP)”,曾经经历过多次“高潮”。虽有成功的经验,但多数企业仍然对于花这么多钱搞ERP是否值得,感到疑惑。因此,当今天因特网、电子商务来临之际,他们就会感到更加... 一、企业的ERP与E-business 在我国,企业实施“企业资源计划(ERP)”,曾经经历过多次“高潮”。虽有成功的经验,但多数企业仍然对于花这么多钱搞ERP是否值得,感到疑惑。因此,当今天因特网、电子商务来临之际,他们就会感到更加困惑:碰到的到底是最令人兴奋的商机,还是最可怕的挑战。 展开更多
关键词 企业资源计划(ERP) e-business 有效结合 电子商务 因特网 高潮 商机
下载PDF
Accelerating Oxygen Electrocatalysis Kinetics on Metal-Organic Frameworks via Bond Length Optimization 被引量:2
14
作者 Fan He Yingnan Liu +10 位作者 Xiaoxuan Yang Yaqi Chen Cheng‑Chieh Yang Chung‑Li Dong Qinggang He Bin Yang Zhongjian Li Yongbo Kuang Lecheng Lei Liming Dai Yang Hou 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第9期279-290,共12页
Metal-organic frameworks(MOFs)have been developed as an ideal platform for exploration of the relationship between intrinsic structure and catalytic activity,but the limited catalytic activity and stability has hamper... Metal-organic frameworks(MOFs)have been developed as an ideal platform for exploration of the relationship between intrinsic structure and catalytic activity,but the limited catalytic activity and stability has hampered their practical use in water splitting.Herein,we develop a bond length adjustment strategy for optimizing naphthalene-based MOFs that synthesized by acid etching Co-naphthalenedicarboxylic acid-based MOFs(donated as AE-CoNDA)to serve as efficient catalyst for water splitting.AE-CoNDA exhibits a low overpotential of 260 mV to reach 10 mA cm^(−2)and a small Tafel slope of 62 mV dec^(−1)with excellent stability over 100 h.After integrated AE-CoNDA onto BiVO_(4),photocurrent density of 4.3 mA cm^(−2)is achieved at 1.23 V.Experimental investigations demonstrate that the stretched Co-O bond length was found to optimize the orbitals hybridization of Co 3d and O 2p,which accounts for the fast kinetics and high activity.Theoretical calculations reveal that the stretched Co-O bond length strengthens the adsorption of oxygen-contained intermediates at the Co active sites for highly efficient water splitting. 展开更多
关键词 Metal-organic frameworks Bond length adjustment Spin state transition Orbitals hybridization Water splitting
下载PDF
Application of VPN in e-Business
15
作者 刘爽英 李博 《Journal of Measurement Science and Instrumentation》 CAS 2012年第3期248-250,共3页
With the development of lnternet, eBusiness has gradually become a new model for business activity, however, the security of eBusiness is a major bottleneck restricting the development of eBusiness. Network with virtu... With the development of lnternet, eBusiness has gradually become a new model for business activity, however, the security of eBusiness is a major bottleneck restricting the development of eBusiness. Network with virtual private net work(VPN) can reduce network costs and communication costs, increase flexibility and provide safe and smooth network for the corporations that do eBusiness across regions. This article introduces the definition and the technological core of VPN, and researches and analyzes the VPN application in ecommerce. 展开更多
关键词 e-business lnternet security virtual private network(VPN) TUNNEL
下载PDF
Managing the surge:A comprehensive review of the entire disposal framework for retired lithium-ion batteries from electric vehicles 被引量:2
16
作者 Ruohan Guo Feng Wang +2 位作者 M.Akbar Rhamdhani Yiming Xu Weixiang Shen 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第5期648-680,共33页
Anticipating the imminent surge of retired lithium-ion batteries(R-LIBs)from electric vehicles,the need for safe,cost-effective and environmentally friendly disposal technologies has escalated.This paper seeks to offe... Anticipating the imminent surge of retired lithium-ion batteries(R-LIBs)from electric vehicles,the need for safe,cost-effective and environmentally friendly disposal technologies has escalated.This paper seeks to offer a comprehensive overview of the entire disposal framework for R-LIBs,encompassing a broad spectrum of activities,including screening,repurposing and recycling.Firstly,we delve deeply into a thorough examination of current screening technologies,shifting the focus from a mere enumeration of screening methods to the exploration of the strategies for enhancing screening efficiency.Secondly,we outline battery repurposing with associated key factors,summarizing stationary applications and sizing methods for R-LIBs in their second life.A particular light is shed on available reconditioning solutions,demonstrating their great potential in facilitating battery safety and lifetime in repurposing scenarios and identifying their techno-economic issues.In the realm of battery recycling,we present an extensive survey of pre-treatment options and subsequent material recovery technologies.Particularly,we introduce several global leading recyclers to illustrate their industrial processes and technical intricacies.Furthermore,relevant challenges and evolving trends are investigated in pursuit of a sustainable end-of-life management and disposal framework.We hope that this study can serve as a valuable resource for researchers,industry professionals and policymakers in this field,ultimately facilitating the adoption of proper disposal practices. 展开更多
关键词 Lithium-ion battery Battery reproposing and recycling Miaieiials recovery technologies Techno-economic issues End-of-life management Disposal framework
下载PDF
Atomically dispersed Mn-N_(x) catalysts derived from Mn-hexamine coordination frameworks for oxygen reduction reaction 被引量:1
17
作者 Guoyu Zhong Liuyong Zou +10 位作者 Xiao Chi Zhen Meng Zehong Chen Tingzhen Li Yongfa Huang Xiaobo Fu Wenbo Liao Shaona Zheng Yongjun Xu Feng Peng Xinwen Peng 《Carbon Energy》 SCIE EI CAS CSCD 2024年第5期114-126,共13页
Metal-organic frameworks recently have been burgeoning and used as precursors to obtain various metal-nitrogen-carbon catalysts for oxygen reduction reaction(ORR).Although rarely studied,Mn-N-C is a promising catalyst... Metal-organic frameworks recently have been burgeoning and used as precursors to obtain various metal-nitrogen-carbon catalysts for oxygen reduction reaction(ORR).Although rarely studied,Mn-N-C is a promising catalyst for ORR due to its weak Fenton reaction activity and strong graphitization catalysis.Here,we developed a facile strategy for anchoring the atomically dispersed nitrogen-coordinated single Mn sites on carbon nanosheets(MnNCS)from an Mn-hexamine coordination framework.The atomically dispersed Mn-N_(4) sites were dispersed on ultrathin carbon nanosheets with a hierarchically porous structure.The optimized MnNCS displayed an excellent ORR performance in half-cells(0.89 V vs.reversible hydrogen electrode(RHE)in base and 0.76 V vs.RHE in acid in half-wave potential)and Zn-air batteries(233 mW cm^(−2)in peak power density),along with significantly enhanced stability.Density functional theory calculations further corroborated that the Mn-N_(4)-C(12)site has favorable adsorption of*OH as the rate-determining step.These findings demonstrate that the metal-hexamine coordination framework can be used as a model system for the rational design of highly active atomic metal catalysts for energy applications. 展开更多
关键词 carbon nanosheets ELECTROCATALYST metal-organic frameworks Mn-N_(4) oxygen reduction reaction Zn-air batteries
下载PDF
Booming Demand in China’s Second-hand Market——2018 Development Report for China Second-hand E-businesses
18
作者 Lynn Yu 《China's Foreign Trade》 2019年第3期47-49,共3页
On June 3rd,JD.com announced that Paipai,its second-hand commodity trade platform,was strategically merged with Aihuishou(Love Recycling)which is one of the largest electronics recycling platforms in China.Recently,th... On June 3rd,JD.com announced that Paipai,its second-hand commodity trade platform,was strategically merged with Aihuishou(Love Recycling)which is one of the largest electronics recycling platforms in China.Recently,the Ecommerce Research Centre of 100ec.cn released the2018 Development Report for China Second-hand E-businesses(hereinafter referred to as the Report),which indicated that as of the end of 2017. 展开更多
关键词 China service Development Report for China Second-hand e-businesses BOOMING DEMAND in China’s Second-hand MARKET
下载PDF
The Challenge of Implementing Information Security Standards in Small and Medium e-Business Enterprises
19
作者 Ja’far Alqatawna 《Journal of Software Engineering and Applications》 2014年第10期883-890,共8页
The dynamic nature of online systems requires companies to be proactive with thwarting information security threats, and to follow a systematic way for managing and evaluating the security of their online services. Th... The dynamic nature of online systems requires companies to be proactive with thwarting information security threats, and to follow a systematic way for managing and evaluating the security of their online services. The existence of security standards is an important factor that helps organisations to evaluate and manage security by providing guidelines and best practices that enable them to follow a standard and systematic way to protect their e-Business activities. However, the suitability of available information security standards for Small and Medium e-Business Enterprises (e-SME) is worth further investigation. In this paper three major security standards including Common Criteria, System Security Engineering-Capability and Maturity Model and ISO/IEC 27001 were analysed. Accordingly, several challenges associated with these standards that may render them difficult to be implemented in e-SME have been identified. 展开更多
关键词 Information Security STANDARDS e-business Small and MEDIUM ENTERPRISES
下载PDF
One stone two birds:electrochemical and colorimetric dual-mode biosensor based on copper peroxide/covalent organic framework nanocomposite for ultrasensentive norovirus detection 被引量:1
20
作者 Guobao Ning Quanmei Duan +6 位作者 Huan Liang Huifang Liu Min Zhou Chunlan Chen Chong Zhang Hui Zhao Canpeng Li 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期920-931,共12页
Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the world.It is urgent to identify the pathogenic microorganism of the diarrhea in short time.In this work,we developed an electroche... Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the world.It is urgent to identify the pathogenic microorganism of the diarrhea in short time.In this work,we developed an electrochemical and colorimetric dual-mode detection for NoV based on the excellent dual catalytic properties of copper peroxide/COF-NH_(2)nanocomposite(CuO_(2)@COF-NH_(2)).For the colorimetric detection,NoV can be directly detected by the naked eye based on CuO_(2)@COF-NH_(2)as a laccase-like nonazyme using“peptide-NoV-antibody”recognition mode.The colorimetric assay displayed a wide and quality linear detection range from 1 copy/mL to 5000 copies/mL of NoV with a low limit of detection(LOD)of 0.125 copy/mL.For the electrochemical detection of NoV,CuO_(2)@COF-NH_(2)showed an oxidation peak of copper ion from Cu^(+)to Cu^(2+)using“peptide-NoV-antibody”recognition mode.The electrochemical assay showed a linear detection range was 1-5000 copies/mL with a LOD of 0.152 copy/mL.It's worthy to note that this assay does not need other electrical signal molecule,which provide the stable and sensitive electrochemial detection for NoV.The electrochemical and colorimetric dual-mode detection was used to detect NoV in foods and faceal samples,which has the potential for improving food safety and diagnosing of NoV-infected diarrhea. 展开更多
关键词 NOROVIRUS Specific peptides Electrochemical and colorimetric assay DUAL-MODE Copper peroxide/covalent organic framework
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部