In order to elucidate the biochemical mechanism of sperm capaeitation, the relationship between plasmalemma Na, K-ATPase, capacitation and acrosome reaction was investigated.Plasmalemma of guinea pig spermatozoa was i...In order to elucidate the biochemical mechanism of sperm capaeitation, the relationship between plasmalemma Na, K-ATPase, capacitation and acrosome reaction was investigated.Plasmalemma of guinea pig spermatozoa was isolated by sucrose gradient centrifugation for the determination of Na, K-ATPase activity. As far as the authors are aware, the Na, K-ATPase activity in plasmalemma of the guinea pig has never been detected. By treating sperm plasmalemma with 0.05% DOC (deoxycholate), enzyme activity could be quantitatively measured. After spermatozoa were incubated in capacitation medium for 8 h, Na, K-ATPase activity was found to be decreased to 35.6% as compared with that before incubation. The spermatozoa incubated for 10.5h in capacitationmedium containing 1 and 5μmol/L ouabain showed 46.5% and 64.4% acrosome reactions respectively, while the acrosome reaction of the control group was only 27.4%. The above results suggest that the decrease in the Na, K-ATPase activity of guinea pig spermatozoa may be a prerequisite for capacitation.Experimental results demonstrated for the first time that Na, K-ATPase activity exists in the sperm plasmalemma of the guinea pig. It was further found that the decrease of Na, K-ATPase activity of plasmalemma enhances sperm capacitation.It is suggested that sperm capacitation in the guinea pig is possibly induced by the decrease in plasmalemma Na, K-ATPaseand, as a consequence, the intracellular Na^+ is increased, which would benefit the exchange of Na_(out)^+/Ca_(in)^+ and the onset of acrosome reaction.展开更多
文摘In order to elucidate the biochemical mechanism of sperm capaeitation, the relationship between plasmalemma Na, K-ATPase, capacitation and acrosome reaction was investigated.Plasmalemma of guinea pig spermatozoa was isolated by sucrose gradient centrifugation for the determination of Na, K-ATPase activity. As far as the authors are aware, the Na, K-ATPase activity in plasmalemma of the guinea pig has never been detected. By treating sperm plasmalemma with 0.05% DOC (deoxycholate), enzyme activity could be quantitatively measured. After spermatozoa were incubated in capacitation medium for 8 h, Na, K-ATPase activity was found to be decreased to 35.6% as compared with that before incubation. The spermatozoa incubated for 10.5h in capacitationmedium containing 1 and 5μmol/L ouabain showed 46.5% and 64.4% acrosome reactions respectively, while the acrosome reaction of the control group was only 27.4%. The above results suggest that the decrease in the Na, K-ATPase activity of guinea pig spermatozoa may be a prerequisite for capacitation.Experimental results demonstrated for the first time that Na, K-ATPase activity exists in the sperm plasmalemma of the guinea pig. It was further found that the decrease of Na, K-ATPase activity of plasmalemma enhances sperm capacitation.It is suggested that sperm capacitation in the guinea pig is possibly induced by the decrease in plasmalemma Na, K-ATPaseand, as a consequence, the intracellular Na^+ is increased, which would benefit the exchange of Na_(out)^+/Ca_(in)^+ and the onset of acrosome reaction.
