Background:The aim of this study was to investigate the influence of marking meth-ods on the outcomes of body composition analysis and provide guidance for the se-lection of marking methods in mouse body composition a...Background:The aim of this study was to investigate the influence of marking meth-ods on the outcomes of body composition analysis and provide guidance for the se-lection of marking methods in mouse body composition analysis.Methods:Male C57BL/6J mice aged 6 weeks were randomly assigned for pre-and post-ear tagging measurements.The body composition of the mice was measured using a small animal body composition analyzer,which provided measurements of the mass of fat,lean,and free fluid.Then,the mass of fat,lean and free fluid to body weight ratio was gained.Further data analysis was conducted to obtain the range and coeffi-cient of variation in body composition measurements for each mouse.The distribution of fat and lean tissue in the mice was also analyzed by comparing the fat-to-lean ratio.Results:(1)The mass of all body composition components in the ear tagging group was significantly lower than that in the control group.(2)There was a significant in-crease in the range and coefficient of variation of body composition measurements between the ear tagging group and the control group.(3)The fat-to-lean ratio in the ear tagging group was significantly lower than that in the control group.Conclusions:Ear tagging significantly lowered the results of body composition analy-sis in mice and higher the results of measurement error.Therefore,ear tagging should be avoided as much as possible when conducting body composition analysis experi-ments in mice.展开更多
【目的】为了适应中国牧场的移动通信环境,加强动物从饲养至屠宰的全程监管,实现对动物的追踪和溯源。【方法】组合"Alien H3"和"Mifare One S70"两种特性不同的芯片,制作一种复合型的RFID动物耳标。其中"H3&q...【目的】为了适应中国牧场的移动通信环境,加强动物从饲养至屠宰的全程监管,实现对动物的追踪和溯源。【方法】组合"Alien H3"和"Mifare One S70"两种特性不同的芯片,制作一种复合型的RFID动物耳标。其中"H3"芯片适宜远距离身份识别,需要应用抗碰撞数学模型实现识别概率的优化。"S70"芯片适宜存储动物饲养日志,为了避免日志被篡改,需要采用"位运算"方式计算适宜的权限控制码。【结果】复合型耳标支持在运动中远距离识别动物身份,算法优化后,单时隙识别概率从0.055 4提升至0.379 8,表现为识别效率提高7倍;耳标还支持近距离扫描更新饲养日志,控制码设置为"70FF0869",保护日志不被篡改的同时实现透明阅读。【结论】复合型耳标可以帮助牧场追踪动物的饲养过程,避免动物在活动中错漏重要的防疫流程,并加强了动物档案的管理;复合型耳标又能支持流通环节的NFC扫描,客户可以通过手机透明地阅读动物饲养日志,实现溯源。展开更多
文摘Background:The aim of this study was to investigate the influence of marking meth-ods on the outcomes of body composition analysis and provide guidance for the se-lection of marking methods in mouse body composition analysis.Methods:Male C57BL/6J mice aged 6 weeks were randomly assigned for pre-and post-ear tagging measurements.The body composition of the mice was measured using a small animal body composition analyzer,which provided measurements of the mass of fat,lean,and free fluid.Then,the mass of fat,lean and free fluid to body weight ratio was gained.Further data analysis was conducted to obtain the range and coeffi-cient of variation in body composition measurements for each mouse.The distribution of fat and lean tissue in the mice was also analyzed by comparing the fat-to-lean ratio.Results:(1)The mass of all body composition components in the ear tagging group was significantly lower than that in the control group.(2)There was a significant in-crease in the range and coefficient of variation of body composition measurements between the ear tagging group and the control group.(3)The fat-to-lean ratio in the ear tagging group was significantly lower than that in the control group.Conclusions:Ear tagging significantly lowered the results of body composition analy-sis in mice and higher the results of measurement error.Therefore,ear tagging should be avoided as much as possible when conducting body composition analysis experi-ments in mice.