[ Objective ] The research aimed to discuss the occurrence regularity of apple early defoliation disease and control effects of fungicides in west Sichuan plateau. [ Method ] Apple early defoliation disease in west Si...[ Objective ] The research aimed to discuss the occurrence regularity of apple early defoliation disease and control effects of fungicides in west Sichuan plateau. [ Method ] Apple early defoliation disease in west Sichuan plateau was systematically investigated during 2009 and 2011, and the control effects of different fungicides during different spraying periods were studied. [ Result] The species of pathogen mainly was Marssonina ma/i （ P. Henn）, accounting for 32.5%, Phyl-losticta Pirina Sa accounted for 29.9%, and Alternaria mali Roberts accounted for 25.7%. The field fluctuation regularity was as follows ： the disease generally be- gan in the mid-May and ended in early and middle October with only one damage peak during the whole year, the fluctuation had positive correlation with rainfall （ R2 =0.891 8 ＊ ＊ ）. Control test showed that 40% flusilazole had the best control effect （86.5%）, which was best to apply after anthesis. [ Conclusion ] The paper provided theoretical basis for the effective control against apple early defoliation disease.展开更多
利用对峙培养法,从分离自番茄根部土壤的细菌中筛选得1株对番茄早疫病菌具强拮抗活性的菌株B731。根据形态特征、生理生化反应及16S r DNA序列分析将其鉴定为枯草芽孢杆菌Bacillus subtilis。该菌在初始p H 7.0的NB培养液、装液量25 m L...利用对峙培养法,从分离自番茄根部土壤的细菌中筛选得1株对番茄早疫病菌具强拮抗活性的菌株B731。根据形态特征、生理生化反应及16S r DNA序列分析将其鉴定为枯草芽孢杆菌Bacillus subtilis。该菌在初始p H 7.0的NB培养液、装液量25 m L(250 m L锥形瓶)、28℃、120 r/min培养24~48 h抑菌能力最大,抑菌带宽达1.24 cm,且其在番茄植株及根际土壤中有很强的定殖能力。接种15 d后,在番茄植株叶片或果实表面,其定殖量可保持在2×102 cfu/cm2以上;而在番茄根际土壤中,其定殖量可达104 cfu/g。菌株B731的代谢产物能有效抑制番茄早疫病菌分生孢子的萌发和菌丝生长,抑制率分别高达89.72%和82.62%。喷施于叶面,具有很好的防病效果,预防处理防效可高达81.83%。展开更多
基金Supported by Comprehensive Test Station in West Sichuan Plateau for National Apple's Industrial Technology System ( CARS-28)~~
文摘[ Objective ] The research aimed to discuss the occurrence regularity of apple early defoliation disease and control effects of fungicides in west Sichuan plateau. [ Method ] Apple early defoliation disease in west Sichuan plateau was systematically investigated during 2009 and 2011, and the control effects of different fungicides during different spraying periods were studied. [ Result] The species of pathogen mainly was Marssonina ma/i （ P. Henn）, accounting for 32.5%, Phyl-losticta Pirina Sa accounted for 29.9%, and Alternaria mali Roberts accounted for 25.7%. The field fluctuation regularity was as follows ： the disease generally be- gan in the mid-May and ended in early and middle October with only one damage peak during the whole year, the fluctuation had positive correlation with rainfall （ R2 =0.891 8 ＊ ＊ ）. Control test showed that 40% flusilazole had the best control effect （86.5%）, which was best to apply after anthesis. [ Conclusion ] The paper provided theoretical basis for the effective control against apple early defoliation disease.
文摘利用对峙培养法,从分离自番茄根部土壤的细菌中筛选得1株对番茄早疫病菌具强拮抗活性的菌株B731。根据形态特征、生理生化反应及16S r DNA序列分析将其鉴定为枯草芽孢杆菌Bacillus subtilis。该菌在初始p H 7.0的NB培养液、装液量25 m L(250 m L锥形瓶)、28℃、120 r/min培养24~48 h抑菌能力最大,抑菌带宽达1.24 cm,且其在番茄植株及根际土壤中有很强的定殖能力。接种15 d后,在番茄植株叶片或果实表面,其定殖量可保持在2×102 cfu/cm2以上;而在番茄根际土壤中,其定殖量可达104 cfu/g。菌株B731的代谢产物能有效抑制番茄早疫病菌分生孢子的萌发和菌丝生长,抑制率分别高达89.72%和82.62%。喷施于叶面,具有很好的防病效果,预防处理防效可高达81.83%。
