Cladosporium cladosporioides is an emerging pathogenic fungus that causes Cladosporium rot in postharvest table grapes(Vitis vinifera). However,studies investigating the infection process of C. cladosporioides are lac...Cladosporium cladosporioides is an emerging pathogenic fungus that causes Cladosporium rot in postharvest table grapes(Vitis vinifera). However,studies investigating the infection process of C. cladosporioides are lacking. Therefore, this study aimed to elucidate the infection process by investigating the influence of microclimatic parameters(temperature, wetness and fungal age) in C. cladosporioides pathogenesis, activities of grape defense-related enzymes and grape quality during the infection. C. cladosporioides effectively infects grapes by developing distinct colonies on the artificial wounds and berry surfaces, completing its life cycle within 48 h. The C. cladosporioides disease incidence optimally occurred at 20 ℃ and 25 ℃. Wetness played an influential role in the infectivity of C. cladosporioides and 7-day-old C. cladosporioides resulted in the most serious disease incidence of table grapes. As a result of infection, the quality of grapes was affected, including berry weight, p H, titratable acidity(TA), total soluble solids(TSS), and ascorbic acid level. This infection also induced defense-related enzymes, including polyphenoloxidase(PPO), peroxidase(POD), phenylalanine ammonialyase(PAL), and catalase(CAT), at certain times. The findings of this study demonstrated that Cladosporium rot development depended on the microclimatic parameters of grapes, significantly affected the grape quality and induced grape's defense-related enzymes.展开更多
Through the investigation of the current production situation,regional distribution,variety structure,production organization and existing problems of table grapes in Henan Province,this paper conducted an in-depth an...Through the investigation of the current production situation,regional distribution,variety structure,production organization and existing problems of table grapes in Henan Province,this paper conducted an in-depth analysis on the advantages and disadvantages of the development of table grapes industry in Henan Province.In view of the current problems and development trend,it came up with some pertinent recommendations,including transforming the concept of cultivation,adhering to the principle of"high-quality varieties,standardized cultivation,mechanized management,large-scale operation,and diversified market",strengthening brand building,and promote the integrated development of the primary,secondary and tertiary industries,so as to promote the change of table grape industry in Henan Province from"quantitative scale model"to"quality benefit model",and promote its sustainable and high-quality development.展开更多
Changes in the metabolites of table grapes(Vitis vinifera) reportedly occur during postharvest senescence. The aim of this study was to determine the metabolomic differences in postharvest table grapes(‘Red Globe’) ...Changes in the metabolites of table grapes(Vitis vinifera) reportedly occur during postharvest senescence. The aim of this study was to determine the metabolomic differences in postharvest table grapes(‘Red Globe’) after being subjected to different senescence periods. To this end, we used widely targeted metabolomics based on ultra-performance liquid chromatography and tandem mass spectrometry. A total of 135 differential metabolites were identified. During postharvest senescence, the levels of most differential flavonoids(e.g., pelargonidin 3-O-glucoside, quercetin-3-O-glucoside, and cyanidin 3-O-glucoside) and L-aspartic acid decreased, while the levels of phenolic acids(e.g., trans-4-hydroxycinnamic acid methyl ester) and pantothenol increased. During early and late senescence, the levels of most differential lipids, especially LysoPC, as well as those of nucleotides and their derivatives, such as uridine, decreased and increased, respectively. Collectively, the findings of this study provide fundamental insights into the reasonable control of table grape fruit postharvest senescence and lay a solid foundation for further research.展开更多
基金the financial support received from National Natural Science Foundation of China (Grant Nos. 31950410556, 31901743, and 31971795)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (Grant No. PAPD-2018-87)。
文摘Cladosporium cladosporioides is an emerging pathogenic fungus that causes Cladosporium rot in postharvest table grapes(Vitis vinifera). However,studies investigating the infection process of C. cladosporioides are lacking. Therefore, this study aimed to elucidate the infection process by investigating the influence of microclimatic parameters(temperature, wetness and fungal age) in C. cladosporioides pathogenesis, activities of grape defense-related enzymes and grape quality during the infection. C. cladosporioides effectively infects grapes by developing distinct colonies on the artificial wounds and berry surfaces, completing its life cycle within 48 h. The C. cladosporioides disease incidence optimally occurred at 20 ℃ and 25 ℃. Wetness played an influential role in the infectivity of C. cladosporioides and 7-day-old C. cladosporioides resulted in the most serious disease incidence of table grapes. As a result of infection, the quality of grapes was affected, including berry weight, p H, titratable acidity(TA), total soluble solids(TSS), and ascorbic acid level. This infection also induced defense-related enzymes, including polyphenoloxidase(PPO), peroxidase(POD), phenylalanine ammonialyase(PAL), and catalase(CAT), at certain times. The findings of this study demonstrated that Cladosporium rot development depended on the microclimatic parameters of grapes, significantly affected the grape quality and induced grape's defense-related enzymes.
基金Characteristic Forest and Fruit Special Project of Science and Technology Support Action Plan for"Regional Distribution,Large-scale Operation,Standardized Production,and Industrialized Development of High-quality Wheat,Peanuts,Grass and Livestock,Fruits and Vegetable Industries in Henan Province(202004)Project for Co-construction of Modern Agriculture Comprehensive Demonstration County between Henan Academy of Agricultural Sciences and the Counties(Yu Nong Ke[2021]No.30).
文摘Through the investigation of the current production situation,regional distribution,variety structure,production organization and existing problems of table grapes in Henan Province,this paper conducted an in-depth analysis on the advantages and disadvantages of the development of table grapes industry in Henan Province.In view of the current problems and development trend,it came up with some pertinent recommendations,including transforming the concept of cultivation,adhering to the principle of"high-quality varieties,standardized cultivation,mechanized management,large-scale operation,and diversified market",strengthening brand building,and promote the integrated development of the primary,secondary and tertiary industries,so as to promote the change of table grape industry in Henan Province from"quantitative scale model"to"quality benefit model",and promote its sustainable and high-quality development.
基金supported by the Natural Science Foundation of China(31801833 and 31801811)the Innovation Capability Support Programs of Shaanxi Province,China(2020KJXX-035)+2 种基金the China Postdoctoral Science Foundation(2019M653771 and 2019T120953)the Fundamental Research Funds for the Central Universities of China(2452019016)the China Agriculture Research System of MOF and MARA(CARS-29-zp-6)。
文摘Changes in the metabolites of table grapes(Vitis vinifera) reportedly occur during postharvest senescence. The aim of this study was to determine the metabolomic differences in postharvest table grapes(‘Red Globe’) after being subjected to different senescence periods. To this end, we used widely targeted metabolomics based on ultra-performance liquid chromatography and tandem mass spectrometry. A total of 135 differential metabolites were identified. During postharvest senescence, the levels of most differential flavonoids(e.g., pelargonidin 3-O-glucoside, quercetin-3-O-glucoside, and cyanidin 3-O-glucoside) and L-aspartic acid decreased, while the levels of phenolic acids(e.g., trans-4-hydroxycinnamic acid methyl ester) and pantothenol increased. During early and late senescence, the levels of most differential lipids, especially LysoPC, as well as those of nucleotides and their derivatives, such as uridine, decreased and increased, respectively. Collectively, the findings of this study provide fundamental insights into the reasonable control of table grape fruit postharvest senescence and lay a solid foundation for further research.
