期刊文献+
共找到21,163篇文章
< 1 2 250 >
每页显示 20 50 100
牛冠状病毒TaqMan荧光定量PCR检测方法的建立及应用
1
作者 蒲鹏 李晨露 +2 位作者 张琪 吴发兴 许信刚 《动物医学进展》 北大核心 2024年第2期7-10,共4页
为建立牛冠状病毒TaqMan荧光定量PCR检测方法,根据GenBank收录的牛冠状病毒AKS-01株N基因序列(KU886219)保守区设计特异性引物和探针,构建重组质粒并进行反应条件优化、特异性试验、重复性试验以及敏感性试验,建立一种检测BCoV的TaqMan... 为建立牛冠状病毒TaqMan荧光定量PCR检测方法,根据GenBank收录的牛冠状病毒AKS-01株N基因序列(KU886219)保守区设计特异性引物和探针,构建重组质粒并进行反应条件优化、特异性试验、重复性试验以及敏感性试验,建立一种检测BCoV的TaqMan荧光定量PCR方法。结果显示,建立的牛冠状病毒TaqMan荧光定量PCR检测方法特异性、敏感性和重复性均良好。该方法BCoV重组质粒标准品在5.75×10^(7)~5.75×10^(3)copies/μL时与Ct值呈现良好线性关系,该方法对牛轮状病毒、牛传染性鼻气管炎病毒、牛病毒性腹泻病毒、牛副流感病毒3型均无交叉反应,特异性良好;该方法对BCoV重组质粒标准品最低检测限为5.75×10^(1)copies/μL;批内和批间重复性试验结果稳定,变异系数均小于2%。利用所建立的TaqMan荧光定量PCR方法对收集的132份样品进行检测,与常规PCR相比,两者符合率为96.21%,可为BCoV的临床检测和流行病学调查提供技术支持。 展开更多
关键词 牛冠状病毒 Taqman荧光定量PCR 检测方法
下载PDF
鸡毒支原体 Taq Man实时荧光定量PCR检测方法的建立
2
作者 孙宇 苗立中 +6 位作者 程立坤 赵家磊 赵修报 沈志强 王敬茹 李书光 余燕 《动物医学进展》 北大核心 2024年第4期64-68,共5页
为快速检测鸡毒支原体,根据鸡毒支原体的保守基因设计特异性引物和Taq Man探针,建立鸡毒支原体Taq Man实时荧光定量PCR方法并分析鸡毒支原体培养过程中颜色单位改变法(color change unit,CCU)和荧光定量PCR检测的相关性。结果显示,标准... 为快速检测鸡毒支原体,根据鸡毒支原体的保守基因设计特异性引物和Taq Man探针,建立鸡毒支原体Taq Man实时荧光定量PCR方法并分析鸡毒支原体培养过程中颜色单位改变法(color change unit,CCU)和荧光定量PCR检测的相关性。结果显示,标准曲线y=-3.646x+44.208,相关系数R^(2)=0.998,最低检测限度为1 copy/μL;与其他菌株等均无交叉反应;组内和组间变异系数均小于3%,表明该方法具有良好的稳定性和可重复性。用建立的实时荧光定量PCR检测方法对26份临床样品进行检测,该方法较普通PCR方法检出率更高。建立的荧光定量PCR与CCU显示鸡毒支原体在对数生长期时,两者具有一定的对应关系。表明建立了鸡毒支原体Taq Man探针实时荧光定量PCR检测方法,可实现对鸡毒支原体的快速检测,为鸡毒支原体感染的防控提供技术基础。 展开更多
关键词 鸡毒支原体 Taq man探针 荧光定量PCR
下载PDF
羊贝氏柯克斯体TaqMan荧光定量PCR检测方法的建立及应用
3
作者 张锐铮 于皓同 +3 位作者 王凯茸 张琪 张淑霞 许信刚 《动物医学进展》 北大核心 2024年第7期6-10,共5页
为建立检测贝氏柯克斯体(Coxiella burnetii)实时荧光定量PCR方法,根据GenBank上收录的C.burnetii RSA439株com1基因序列(CP040059.1)保守区设计特异性引物和探针,并构建重组质粒及优化反应条件,以期建立一种检测贝氏柯克斯体的Taq Man... 为建立检测贝氏柯克斯体(Coxiella burnetii)实时荧光定量PCR方法,根据GenBank上收录的C.burnetii RSA439株com1基因序列(CP040059.1)保守区设计特异性引物和探针,并构建重组质粒及优化反应条件,以期建立一种检测贝氏柯克斯体的Taq Man荧光定量PCR方法。结果显示,该方法所建立标准曲线线性关系良好;敏感性试验结果显示,最低检出限为2.45×10^(2) copies/μL,与PCR最低检出限为2.45×10^(4) copies/μL相比较,灵敏度提高了100倍;对羊支原体、羊流产衣原体、羊布鲁氏菌等均无交叉反应,批内和批间重复性试验结果稳定,变异系数均小于3%,说明特异性、重复性好;利用该方法对收集的126份样品进行检测,结果显示样品阳性率为9.52%,常规PCR检测阳性率为7.14%,符合率为90.47%。该方法可为贝氏柯克斯体临床上快速检测及防控提供技术支持。 展开更多
关键词 贝氏柯克斯 Taq man荧光定量PCR 检测方法
下载PDF
猪肺炎支原体和猪鼻支原体TaqMan双重荧光PCR检测方法的建立及应用 被引量:1
4
作者 周颖 赵硕 +10 位作者 覃国喜 梁龙华 肖婷 陈忠伟 卢冰霞 秦毅斌 林昌华 张胜斌 段群棚 胡庭俊 何颖 《动物医学进展》 北大核心 2024年第2期11-16,共6页
为建立一种可快捷鉴别检测猪肺炎支原体(Mhp)和猪鼻支原体(Mhr)的方法,针对Mhp的183基因和Mhr的p37基因保守片段,分别设计合成引物及TaqMan探针,优化反应条件,建立一种可同时检测Mhp和Mhr的TaqMan双重荧光PCR方法,并评价其特异性、敏感... 为建立一种可快捷鉴别检测猪肺炎支原体(Mhp)和猪鼻支原体(Mhr)的方法,针对Mhp的183基因和Mhr的p37基因保守片段,分别设计合成引物及TaqMan探针,优化反应条件,建立一种可同时检测Mhp和Mhr的TaqMan双重荧光PCR方法,并评价其特异性、敏感性和重复性。结果表明,该方法特异性强,检测其他常见猪呼吸系统病原不发生交叉反应;对标准品pMD18-T-Mhp-183、pMD18-T-Mhr-P37的最低检测限分别达45.2 copies/μL和29.7 copies/μL,比普通PCR检测灵敏度提高10^(3)~10^(4)倍;该方法检测结果的批内与批间变异系数均小于2%。用该方法检测145份广西自治区内临床样品,从中检出82份Mhp和9份Mhr阳性样品。该方法可用于临床样品快速检测及实验室Mhp和Mhr的鉴定,可为Mhp和Mhr在猪群中的早期监测提供技术支持。 展开更多
关键词 猪肺炎支原体 猪鼻支原体 Taqman荧光定量PCR
下载PDF
circMAN1A2调节miR-212-3p/DDIT4轴对胃癌细胞增殖、侵袭和迁移的影响
5
作者 曹博威 贾腾飞 +2 位作者 宋钉町 赵昱清 张文斌 《胃肠病学和肝病学杂志》 CAS 2024年第7期868-874,共7页
目的探究circMAN1A2靶向miR-212-3p/DNA损伤诱导转录子4(NDA damage-inducible transcript 4,DDIT4)轴对胃癌细胞增殖、侵袭和迁移的影响。方法qRT-PCR法检测胃癌组织和细胞中circMAN1A2、miR-212-3p表达水平。荧光素酶检测circMAN1A2与... 目的探究circMAN1A2靶向miR-212-3p/DNA损伤诱导转录子4(NDA damage-inducible transcript 4,DDIT4)轴对胃癌细胞增殖、侵袭和迁移的影响。方法qRT-PCR法检测胃癌组织和细胞中circMAN1A2、miR-212-3p表达水平。荧光素酶检测circMAN1A2与miR-212-3p、DDIT4和miR-212-3p的靶向关系。在胃癌细胞SGC-7901、HGC-27中转染si-NC、si-circMAN1A2、si-circMAN1A2+anti-NC、si-circMAN1A2+anti-miR-212-3p、miR-NC、miR-212-3p mimics,将未转染的SGC-7901、HGC-27细胞设为对照。平板克隆实验检测细胞增殖;细胞划痕及Transwell实验分别检测细胞迁移、侵袭能力。Western blotting检测增殖、迁移及侵袭相关蛋白表达。结果在胃癌组织及细胞中,circMAN1A2高表达,miR-212-3p低表达;下调circMAN1A2可以靶向上调miR-212-3p表达,且下调DDIT4表达,进而抑制胃癌细胞增殖、侵袭和迁移能力。抑制miR-212-3p表达可部分逆转抑制circMAN1A2表达对胃癌细胞恶性增殖的抑制作用。结论在胃癌细胞中抑制circMAN1A2表达可抑制胃癌细胞增殖、迁移与侵袭,其与调节miR-212-3p/DDIT4信号轴有关。 展开更多
关键词 环状RNA man1A2 微小RNA-212-3p DNA损伤诱导转录子4 胃癌 增殖 侵袭 迁移
下载PDF
猪细小病毒TaqMan荧光定量PCR检测方法的建立及应用
6
作者 杨奕 吴发兴 +4 位作者 康京丽 孙洪涛 王志亮 许信刚 张琪 《动物医学进展》 北大核心 2024年第2期75-79,共5页
旨在建立一种猪细小病毒(PPV)快速准确PCR检测方法,根据PPV VP2基因的保守序列设计并合成1对特异性引物和1条特异性探针,建立了可检测PPV的TaqMan荧光定量PCR检测方法。建立的检测方法敏感性高,最低可检测8.76×10^(1)copies/μL的P... 旨在建立一种猪细小病毒(PPV)快速准确PCR检测方法,根据PPV VP2基因的保守序列设计并合成1对特异性引物和1条特异性探针,建立了可检测PPV的TaqMan荧光定量PCR检测方法。建立的检测方法敏感性高,最低可检测8.76×10^(1)copies/μL的PPV VP2基因标准品;特异性试验结果显示,该方法检测猪流行性腹泻病毒、猪圆环病毒2型、伪狂犬病病毒、猪繁殖与呼吸综合征病毒均无交叉反应;重复性试验组内与组间变异系数均低于2%,重复性良好。用该方法检测102份临床样本,检出阳性样本16份,阳性检出率为15.69%,将检测出的阳性样本进行PCR扩增并测序,证实检测样品中确实存在PPV。该方法敏感性高、特异性强,适用于PPV的定量检测及猪细小病毒病的流行病学调查。 展开更多
关键词 猪细小病毒 VP2 TAQman探针 荧光定量PCR
下载PDF
牛支原体和丝状支原体丝状亚种双重TaqMan荧光定量PCR检测方法的建立与应用
7
作者 马芷忻 武琪 +2 位作者 刘桐 辛九庆 徐青元 《中国预防兽医学报》 CAS CSCD 北大核心 2024年第3期262-268,共7页
牛传染性胸膜肺炎和牛支原体病均为重要的牛传染病,两者均可致牛出现呼吸系统症状,临床上难以区分。为建立鉴别诊断牛传染性胸膜肺炎和牛支原体病的双重Taq Man荧光定量PCR检测方法,本研究根据这两种病的病原丝状支原体丝状亚种和牛支... 牛传染性胸膜肺炎和牛支原体病均为重要的牛传染病,两者均可致牛出现呼吸系统症状,临床上难以区分。为建立鉴别诊断牛传染性胸膜肺炎和牛支原体病的双重Taq Man荧光定量PCR检测方法,本研究根据这两种病的病原丝状支原体丝状亚种和牛支原体的基因组保守区域(丝状支原体丝状亚种nt826-nt1742,牛支原体nt189-nt618)分别设计引物与探针,通过优化反应体系与反应条件,建立同时检测这两种病原的Taq Man荧光定量PCR检测方法。特异性试验结果显示,该方法仅对丝状支原体丝状亚种模拟样品和牛支原体检测结果为阳性,而巴氏杆菌、牛传染性鼻气管炎病毒、无乳支原体、山羊支原体山羊肺炎亚种、丝状支原体山羊亚种、关节炎支原体Leachii株检测结果均为阴性,特异性较强。分别以1.0×10~7拷贝/μL~1.0×10~1拷贝/μL的p EASY-Mmm和p EASY-Mb质粒标准品为模板,进行敏感性试验,结果显示,该方法对丝状支原体丝状亚种和牛支原体重组质粒标准品的检测限均为1.0×10~1拷贝/μL,敏感性较高。对不同浓度质粒标准品混合物的重复性试验结果显示,组内与组间重复性试验变异系数均小于2.5%,重复性较好。利用该方法对112份临床样品(104份鼻拭子、8份肺组织样品)检测,结果显示,丝状支原体丝状亚种检测结果和已发表PCR方法检测结果一致,均为阴性,而本实验建立的方法检测出4份牛支原体阳性样品,且经测序证实检出样品为真实阳性样品,而已发表的多重PCR方法检测该病原结果均为阴性。本研究建立了能够同时检测丝状支原体丝状亚种和牛支原体的双重Taq Man荧光定量PCR方法,其特异性强、敏感性高、重复性好,可用于各种临床样品的检测,为这两种病原的快速检测和流行病学调查提供了技术手段。 展开更多
关键词 牛传染性胸膜肺炎 牛支原体 Taqman荧光定量PCR
下载PDF
TaqMan探针双重实时荧光定量PCR法同步检测兰花中的建兰花叶病毒和齿兰环斑病毒
8
作者 孙爱青 王丽花 +3 位作者 张艺萍 杨秀梅 许凤 苏艳 《植物保护》 CAS CSCD 北大核心 2024年第2期219-227,239,共10页
为建立一种同步定量检测建兰花叶病毒(cymbidium mosaic virus,CymMV)和齿兰环斑病毒(odontoglossum ringspot virus,ORSV)的高效检测方法,本研究根据CymMV和ORSV CP基因高度保守区分别设计引物和探针并筛选,获得156 bp和148 bp的靶标... 为建立一种同步定量检测建兰花叶病毒(cymbidium mosaic virus,CymMV)和齿兰环斑病毒(odontoglossum ringspot virus,ORSV)的高效检测方法,本研究根据CymMV和ORSV CP基因高度保守区分别设计引物和探针并筛选,获得156 bp和148 bp的靶标序列及对应的最优特异性引物探针组合,建立了基于TaqMan探针的双重实时荧光定量PCR方法。该方法最低检出限为1拷贝或6.2×10^(-3)fg,最低稳定检出限为10拷贝或6.2×10^(-2)fg,是RT-PCR的10~100倍;构建的标准曲线线性关系良好,扩增效率分别为97.7%和100.2%,相关系数R2分别为1.000和0.999;对其他5种常见病毒均无扩增曲线,检测特异性强;批组内与批组间重复性试验Ct值变异系数≤0.60%,重复性和稳定性好。利用该方法和基因芯片法分别对4个种属的66个兰花样品进行方法验证,该方法相较于基因芯片法检出率提高了53.85%(CymMV)和162.5%(ORSV)。综上所述,本方法可同时高通量检测CymMV和ORSV 2种病毒靶基因,结果可靠,应用前景广阔,可为开展病毒精准鉴定、科学防控以及从源头遏制病毒传播提供技术支撑。 展开更多
关键词 建兰花叶病毒 齿兰环斑病毒 TAQman探针 RT-QPCR 兰花
下载PDF
Fast solution to the free return orbit's reachable domain of the manned lunar mission by deep neural network
9
作者 YANG Luyi LI Haiyang +1 位作者 ZHANG Jin ZHU Yuehe 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第2期495-508,共14页
It is important to calculate the reachable domain(RD)of the manned lunar mission to evaluate whether a lunar landing site could be reached by the spacecraft. In this paper, the RD of free return orbits is quickly eval... It is important to calculate the reachable domain(RD)of the manned lunar mission to evaluate whether a lunar landing site could be reached by the spacecraft. In this paper, the RD of free return orbits is quickly evaluated and calculated via the classification and regression neural networks. An efficient databasegeneration method is developed for obtaining eight types of free return orbits and then the RD is defined by the orbit’s inclination and right ascension of ascending node(RAAN) at the perilune. A classify neural network and a regression network are trained respectively. The former is built for classifying the type of the RD, and the latter is built for calculating the inclination and RAAN of the RD. The simulation results show that two neural networks are well trained. The classification model has an accuracy of more than 99% and the mean square error of the regression model is less than 0.01°on the test set. Moreover, a serial strategy is proposed to combine the two surrogate models and a recognition tool is built to evaluate whether a lunar site could be reached. The proposed deep learning method shows the superiority in computation efficiency compared with the traditional double two-body model. 展开更多
关键词 manned lunar mission free return orbit reachable domain(RD) deep neural network computation efficiency
下载PDF
DOPO-MAN改性大豆油基水性聚氨酯丙烯酸酯的制备
10
作者 秦良雨 彭丽 王念贵 《胶体与聚合物》 CAS 2024年第2期78-81,共4页
本文以9,10-二氢-9-氧杂-10-磷杂菲-10-氧化物(DOPO)、马来酸酐(MA)和二乙醇胺(DEA)为原料制备出DOPO-MAN,以其为改性剂制备出可UV-LED固化的DOPO-MAN改性大豆油基水性聚氨酯丙烯酸酯乳液。将乳液与光引发剂(TPO)混合均匀后经UV-LED光固... 本文以9,10-二氢-9-氧杂-10-磷杂菲-10-氧化物(DOPO)、马来酸酐(MA)和二乙醇胺(DEA)为原料制备出DOPO-MAN,以其为改性剂制备出可UV-LED固化的DOPO-MAN改性大豆油基水性聚氨酯丙烯酸酯乳液。将乳液与光引发剂(TPO)混合均匀后经UV-LED光固化,制成固化膜。实验结果表明,当DOPO-MAN的含量为20%时,固化膜的极限氧指数为24.3,UL-94测试也达到了V-1级别。 展开更多
关键词 水性聚氨酯 大豆油 DOPO-man 改性
下载PDF
Assessment of Meteorological Threats to the Coordinated Search and Rescue of Unmanned/Manned Aircraft
11
作者 Fei YAN Chuan LI +2 位作者 Xiaoyi FU Kefeng WU Yuying LI 《Meteorological and Environmental Research》 2024年第1期27-29,37,共4页
The architecture and working principle of coordinated search and rescue system of unmanned/manned aircraft,which is composed of manned/unmanned aircraft and manned aircraft,were first introduced,and they can cooperate... The architecture and working principle of coordinated search and rescue system of unmanned/manned aircraft,which is composed of manned/unmanned aircraft and manned aircraft,were first introduced,and they can cooperate with each other to complete a search and rescue task.Secondly,a threat assessment method based on meteorological data was proposed,and potential meteorological threats,such as storms and rainfall,can be predicted by collecting and analyzing meteorological data.Finally,an experiment was carried out to evaluate the performance of the proposed method in different scenarios.The experimental results show that the coordinated search and rescue system of unmanned/manned aircraft can be used to effectively assess meteorological threats and provide accurate search and rescue guidance. 展开更多
关键词 Unmanned/manned aircraft Coordinated search and rescue Assessment of meteorological threats Meteorological data
下载PDF
基于TaqMan MGB探针的大豆北方茎溃疡病菌快速检测
12
作者 李雪莲 于洪娟 +1 位作者 朱小琼 段维军 《大豆科学》 CAS CSCD 北大核心 2024年第3期317-325,共9页
为准确快速检测检疫性真菌大豆北方茎溃疡病菌,根据大豆北方茎溃疡病菌及其近似种的模式分离物ITS序列差异,设计并合成1对特异性引物和1条MGB探针,建立大豆北方茎溃疡病菌的实时荧光PCR检测方法。特异性试验结果表明:该检测方法能特异... 为准确快速检测检疫性真菌大豆北方茎溃疡病菌,根据大豆北方茎溃疡病菌及其近似种的模式分离物ITS序列差异,设计并合成1对特异性引物和1条MGB探针,建立大豆北方茎溃疡病菌的实时荧光PCR检测方法。特异性试验结果表明:该检测方法能特异性检测大豆北方茎溃疡病菌。灵敏度试验结果表明:最低检测限量为10μL反应体系中总DNA含量1.0 pg;实时荧光PCR优化反应条件为引物终浓度0.5μmol·L^(-1),探针终浓度0.6μmol·L^(-1)。实际样品检测结果表明:该方法可用于疑似受大豆北方茎溃疡病菌侵染的进境大豆样品的检测与初筛。此方法准确、快速、灵敏,整个反应过程约1 h,检测过程完全闭管,无需PCR后续处理,可作为大豆北方茎溃疡病菌检测防控方法。 展开更多
关键词 大豆北方茎溃疡病菌 实时荧光PCR Taq man-MGB探针 快速检测
下载PDF
Eco-economic evolution, emergy evaluation and policy options for the sustainable development of Tibet 被引量:13
13
作者 YAN Mao-chao, H. T. Odum ( Commission for Integrated Survey of Natural Resources, Chinese Academy of Sciences, Beijing, China, Center for Environmental Policy, Department of Environmental Engineering Sciences, University of Florida, Gainesville, FL, USA) 《Journal of Geographical Sciences》 SCIE CSCD 2000年第1期1-27,共27页
As economies and ecological support systems become more interdependent, new disciplines are needed to 'bridge the gap' of understanding between societies and nature. It is now clear that neither ecology nor ec... As economies and ecological support systems become more interdependent, new disciplines are needed to 'bridge the gap' of understanding between societies and nature. It is now clear that neither ecology nor economics alone can address the problems of our global commons. New measures of wealth, of value, of contributions and production are needed that acknowledge the 'natural capital' and 'ecosystem services' provided from healthy environments. A new interface is now being recognized termed 'eco-economics'. It is an ambitious and necessary attempt to understand the affairs of humanity and nature as a single, interdependent system. New tools are being invested to measure wealth. services and production fairly and equitably. In this report we use systems analysis approach to study the combined ecological-economic system of Tibet We use an alternative measure of value, based on real contributions to system performance, termed EMERGY, spelt with an 'M'. It is an accounting unit of total contributions, direct and indirect. used in generating a product or service. It is a concept derived from understanding whole systems, their interactions and interdependence, and the resources driving and maintaining them. This broader approach will help us to investigate Tibetan resources utilization and potentialities and exchange. Emergy analysis allows comparison and incroporation of environmental costs and benefits with variables of traditional economic costs and benefits to provide a more comprehensive perspective for public policy directives. Through this research, we think: 1) China has made a great effort in making Tibet develop from a closed regional system to a more and more opened regional system which is very important to the sustainable development of Tibet. 2) The increase of economic development and population growth has a close relationship with the total emergy storage and use in transportation. Education in modern science and technology increased the shared emergy of Tibetans to modern science and technology and stimulated the process of matching material and energy imports from outside with local resources. 3) There is great potential to develop hydropower and geothermal reserves that may stimulate not only economic development but also the better use of environment. 4) The better trained population can increase the empower of Tibet eco-economic system. Future development needs all kinds of talent exchanges with the outside system to develop education. 3) Compared with the Tibet economic system, Taiwan economic system is much more competitive. GNP and emergy use are very useful functions for comparing the competitive power of different ecological economic systems. 展开更多
关键词 EMERGY eco-economic evolution sustainable development
下载PDF
薄壳山核桃疮痂病菌TaqMan荧光定量PCR检测方法的建立及应用
14
作者 赵玉强 卓可儿 +5 位作者 郭云 朱灿灿 王行军 陆小美 田艳丽 胡白石 《植物保护》 CAS CSCD 北大核心 2023年第3期193-198,共6页
疮痂病是薄壳山核桃上最具毁灭性的病害,带菌植物材料是传播疮痂病的重要来源。准确、灵敏、快速的检测方法可为该病害流行规律调查和防控提供有力的依据。本文通过比较薄壳山核桃疮痂病菌Venturia effusa及其近似种之间的ITS序列差异,... 疮痂病是薄壳山核桃上最具毁灭性的病害,带菌植物材料是传播疮痂病的重要来源。准确、灵敏、快速的检测方法可为该病害流行规律调查和防控提供有力的依据。本文通过比较薄壳山核桃疮痂病菌Venturia effusa及其近似种之间的ITS序列差异,设计了特异性引物和TaqMan探针,建立了薄壳山核桃疮痂病菌的荧光定量PCR检测方法。特异性检测结果表明,该方法可以检测不同地区的薄壳山核桃疮痂病菌菌株,而对其近似种以及薄壳山核桃上的其他真菌均没有信号。本研究建立的检测方法对薄壳山核桃疮痂病菌DNA的最低检测限可达0.5 pg/μL。该方法用于田间样品检测时,检测时间仅需1 h,远快于常规的分离培养法。本研究建立的基于TaqMan探针的荧光定量PCR检测方法为薄壳山核桃疮痂病菌的快速检测和监测提供了有力工具。 展开更多
关键词 薄壳山核桃疮痂病菌 荧光定量PCR TAQman探针 快速检测
下载PDF
Degree of coupling and coordination of eco-economic system and the influencing factors: a case study in Yanchi County, Ningxia Hui Autonomous Region, China 被引量:12
15
作者 LU Huiling ZHOU Lihua +2 位作者 CHEN Yong AN Yiwei HOU Caixia 《Journal of Arid Land》 SCIE CSCD 2017年第3期446-457,共12页
Based on the statistical data, we analyzed and evaluated the degree of coupling and coordination of the eco-economic system in Yanchi County for the period spanning from 1983 to 2014. The eco-economic system can be di... Based on the statistical data, we analyzed and evaluated the degree of coupling and coordination of the eco-economic system in Yanchi County for the period spanning from 1983 to 2014. The eco-economic system can be divided into socioeconomic and ecological sub-systems and their relationship can reveal the interaction state between the two sub-systems and help the local government to establish a coordinated development mode. An index system was constructed to assess the development of the two sub-systems before the evaluation of the degree of coupling and coordination. The principal component regression analysis was adopted to quantitatively assess the influences of natural, economic and social factors on the degree of coupling and coordination of the eco-economic system. Results showed that, from 1983 to 2014, the development trends of both sub-systems were increasing with the ecological sub-system having more fluctuations. The degree of coupling and coordination of the eco-economic system in the study area increased gradually from 1983 to 2014, but experienced five different development stages from the verge of disorder to favorable coordination. The development of the local social and economic conditions was the most important factor influencing the degree of coupling and coordination. The second most important factor was the financial support from the local government. In addition, the environment protection policies also played undeniable roles. Due to the diversity of the influence factors, the government should take comprehensive measures to promote the sustainable development of the eco-economic system. 展开更多
关键词 degree of coupling and coordination eco-economic system ecological sub-system socioeconomic sub-system YanchiCounty
下载PDF
Research on Functional Characteristics of Karst Eco-economic Compound System in Guizhou Province 被引量:2
16
作者 LUO Kun-yan Xingyi Normal University for Nationalities,Xingyi 562400,China 《Asian Agricultural Research》 2011年第3期113-119,共7页
Based on the eco-economy theory,this paper analyzed the characteristics of the components of eco-economic compound system of karst region in Guizhou Province.The functional characteristics of eco-economic compound sys... Based on the eco-economy theory,this paper analyzed the characteristics of the components of eco-economic compound system of karst region in Guizhou Province.The functional characteristics of eco-economic compound system of karst region in Guizhou Province were analyzed.The functional characteristics were as follows:low biological production of Guizhou Province;weak capacity of ecosystem;slow speed of storage and accumulation of material and serious environmental pollution;low production and efficiency of energy;serious wastes of energy.On the basis of functional characteristics of eco-economic compound System in Karst region.Some views in terms of maintenance and reconstruction of compound system,were put forward,including laying stress on improving ecological system;choosing and cultivating the advanced species that suit the Karst region;improving the amount and speed of material accumulation,at the same time,introducing into advanced production technologies and management experience;reducing the energy efficiency of each section in economic system and improving the transformation efficiency of energy. 展开更多
关键词 eco-economic COMPOUND system KARST region of Guizh
下载PDF
结核分枝杆菌复合群、副结核分枝杆菌和禽分枝杆菌禽亚种多重TaqMan荧光定量PCR方法的建立及初步应用 被引量:1
17
作者 蔡珠明 党光辉 +6 位作者 臧鑫鑫 邵明珠 唐阳阳 鹿萍 崔莹莹 崔子寅 刘思国 《中国预防兽医学报》 CAS CSCD 北大核心 2023年第1期51-59,共9页
结核分枝杆菌复合群(MTBC)、包括结核分枝杆菌(MTB)、牛分枝杆菌(MB)、非洲分枝杆菌、田鼠分枝杆菌等;非结核分枝杆菌(NTM)中禽分枝杆菌(MA)是自然界常见的动物致病菌,包含4个亚种,分别为禽分枝杆菌禽亚种(MAA)、禽分枝杆菌人/猪亚种(M... 结核分枝杆菌复合群(MTBC)、包括结核分枝杆菌(MTB)、牛分枝杆菌(MB)、非洲分枝杆菌、田鼠分枝杆菌等;非结核分枝杆菌(NTM)中禽分枝杆菌(MA)是自然界常见的动物致病菌,包含4个亚种,分别为禽分枝杆菌禽亚种(MAA)、禽分枝杆菌人/猪亚种(MAH)、禽分枝杆菌副结核亚种/副结核分枝杆菌(MAP)和禽分枝杆菌森林土壤亚种(MAS)。为建立MTBC、MAP和MAA的多重荧光定量PCR检测方法,本研究基于MTBC、MAP和MAA的特异性基因devR、F57和IS901分别设计引物及探针,通过优化各反应条件,建立了一种可同时检测3种分枝杆菌的多重Taq Man荧光定量PCR方法。特异性试验结果显示,该方法对MTB、MB、MAP、MAA和MAS检测为阳性,对其他畜禽病原菌,包括MAH、13种NTM和7种非分枝杆菌检测结果均为阴性,特异性较强。敏感性试验结果显示,该方法对MB、MAP和MAA重组质粒标准品的检测限均为1.0拷贝/μL,敏感性较高;重复性试验结果显示,批内与批间重复性试验变异系数均小于2.5%,重复性较好。利用该方法检测体外模拟污染(MB、MAP和MAA菌液浓度分别为1.0×10^(3)cfu/mL~1.0×10^(0)cfu/mL)的27份牛抗凝血和12份牛淋巴结组织样品,确定该方法对3种菌的检测限,结果显示该方法对3种菌的检测限均为1.0 cfu/mL~10.0 cfu/mL。将浓度为1.0×10^(6)cfu/mL的MB、MAP和MAA菌液分别单一、两两混合、三种菌混合感染BALB/c小鼠,5 d后,剖杀各组小鼠并取各组织及血液样品,同时采用该方法、常规单一PCR及细菌分离培养方法检测,比较三者的检测结果。结果显示,多重荧光定量PCR方法对不同感染组小鼠的各组织样品检出率为98.1%(212/216),常规单一PCR方法的检出率为81.5%(176/216),细菌分离培养的检出率为52.8%(114/216),多重荧光定量PCR与常规单一PCR的阳性符合率均为100.0%(176/176),与细菌分离培养鉴定结果的阳性符合率均为100.0%(114/114)。本研究首次建立了能够同时检测MTBC、MAP和MAA的Taq Man多重荧光定量PCR方法,其特异性强、敏感性高、重复性及稳定性好,检测性能强,可以用于临床各种样品的检测,为这3种病原的快速检测和流行病学调查提供了技术手段。 展开更多
关键词 结核分枝杆菌复合群 副结核分枝杆菌 禽分枝杆菌禽亚种 多重Taq man荧光定量PCR
下载PDF
番茄褐色皱果病毒Taq Man荧光定量RT-PCR检测方法的建立及应用 被引量:1
18
作者 李献锋 于璇 +7 位作者 冯黎霞 于翠 丁钿 徐强 赵菊鹏 马骏 武目涛 魏霜 《植物保护》 CAS CSCD 北大核心 2023年第6期185-193,共9页
为建立番茄褐色皱果病毒(tomato brown rugose fruit virus,ToBRFV)Taq Man荧光定量RT-PCR方法,本研究针对ToBRFV RNA依赖的RNA聚合酶(RNA-dependent RNA polymerase,RdRP)基因序列设计了4组特异性引物和Taq Man探针,构建了标准曲线,并... 为建立番茄褐色皱果病毒(tomato brown rugose fruit virus,ToBRFV)Taq Man荧光定量RT-PCR方法,本研究针对ToBRFV RNA依赖的RNA聚合酶(RNA-dependent RNA polymerase,RdRP)基因序列设计了4组特异性引物和Taq Man探针,构建了标准曲线,并对该方法的特异性、灵敏度和实际样品的检测效率进行评估。结果显示,该方法特异性强,与番茄花叶病毒、番茄斑驳花叶病毒、烟草花叶病毒、黄瓜绿斑驳花叶病毒、番茄环斑病毒、番茄黑环病毒、辣椒轻斑驳病毒、烟草环斑病毒和凤果花叶病毒等9种病毒均无交叉反应。建立的标准曲线的R^(2)为0.999,扩增效率为102.096%,检测灵敏度为10 fg/μL,与EPPO PM 7/146(1)推荐的CaTa28、CSP1325方法相当,是常规RT-PCR的100倍;采用该方法成功从60份番茄和辣椒叶片、种子样品中检出10份阳性样品。建立的Taq Man荧光定量RT-PCR方法特异性强、灵敏度高,适用于实际样品中ToBRFV的快速精准检测。 展开更多
关键词 番茄褐色皱果病毒 RNA依赖的RNA聚合酶 Taq man探针 荧光定量RT-PCR 检测
下载PDF
Yield of Agricultural Eco-economic System in Dongting Rim
19
作者 Maozhang HOU Yulin ZHU 《Asian Agricultural Research》 2013年第10期61-67,共7页
To analyze the efficiency of the agro-ecosystem of Dongting Rim in Hunan Province,this paper adopted the emergy theory to study the agricultural output of this region during 2001 to 2010.The results showed that the va... To analyze the efficiency of the agro-ecosystem of Dongting Rim in Hunan Province,this paper adopted the emergy theory to study the agricultural output of this region during 2001 to 2010.The results showed that the value of regional agricultural emergy output increased by44.68%to(6.50 E)sej,but lower than the growth of non-agricultural industry output.Compared with 2001,the emergy output of planting industry was(4.10E)sej in 2010 which occupied 63.09%of the total agricultural emergy output and got an increase of 2.93 percentage points,while the emergy output of stock farming got a decrease of 2.87 percentage points.The growth of fishery emergy output was not in conformity with the characteristics of Dongting Rim in Hunan.From the contrast between emergy output and economic output,we could find that the economic return ratio of planting industry and fishery industry were lower than those of forestry industry and stock farming industry,which indicated that it is necessary to improve the economic benefits of planting industry and fishery industry which were the traditional industries with advantages of the Dongting Rim in Hunan. 展开更多
关键词 Dongting RIM AGRICULTURAL eco-economic SYSTEM Emer
下载PDF
Emergy Analysis of Sustainability of Eco-economic System in Sichuan's Baisha River Valley
20
作者 AN Yun 《Asian Agricultural Research》 2012年第6期66-70,共5页
Using emergy analysis method,I conduct quantitative analysis of social,economic and natural systems in Sichuan's Baisha River valley during the period 2002-2006,through the establishment of indicators.I compare th... Using emergy analysis method,I conduct quantitative analysis of social,economic and natural systems in Sichuan's Baisha River valley during the period 2002-2006,through the establishment of indicators.I compare the emergy of Baisha River valley,with that of Xinjiang's Manas River valley and Sichuan Province,and evaluate the sustainable development conditions in Baisha River valley,in order to provide corresponding reference for the development and utilization of Baisha River valley in the future.The results show that from the perspective of emergy input of economic system,Baisha River valley is a typical ecological and economic zone with rich resources to be developed;in terms of the development of natural conditions and utilization of ecological resources,the resource utilization intensity of Baisha River valley is weak and the environmental loading ratio there is low,thus the level of emergy yield needs to be improved;the system of Baisha River valley has enormous capacity for sustainable development and good potential for development. 展开更多
关键词 Baisha River valley eco-economic system Emergy analysis Sustainable development
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部