The kinetics of ecotoxicant transport through the membrane was studied. The ecotoxicants studied were pecticides widely used in agriculture: lontrel (clopyralid), sencor (metribuzin), basagran (bentazon), roundup (gly...The kinetics of ecotoxicant transport through the membrane was studied. The ecotoxicants studied were pecticides widely used in agriculture: lontrel (clopyralid), sencor (metribuzin), basagran (bentazon), roundup (glyphosate), kusagard (alloxydim sodium), and sethoxydim, as well as lontrel complexes with cobalt and copper. All compounds considered penetrate through the model phosphatidylcholine liposomal membranes. The transfer rate was monitored by fluorescence quenching of ?-ATP inside the liposomes. The mathematical model for the process was proposed, and the mass transfer rates were calculated. The octanol/water partition constants were determined. All the compounds con- sidered were shown to accumulate in the fatty layer. The kinetics of their accumulation was studied, and the rates of accumulation in the nonpolar phase were calculated, which correlate with the complexation constants of the same compounds. Bioaccumulation of the toxicants is caused by the formation of complexes with the fatty phase (lipid part) of the cellular membranes. The toxicants under study transferred into the nonpolar phase with a considerable rate dur- ing the whole observation time, namely, 18 months.展开更多
The analysis was made over the leukograms of leukocyte concentrate in venous blood of healthy people （77 persons） and a group consisting from 18 persons with hybrid cells （LE-phenomenon） detected in them. Authors ...The analysis was made over the leukograms of leukocyte concentrate in venous blood of healthy people （77 persons） and a group consisting from 18 persons with hybrid cells （LE-phenomenon） detected in them. Authors constructed in vitro processes, which take place in vivo, and got the artificial morphological analogue of LE-cells through a physical and chemical agent--polyethylene glycol. The suggestion was made that the formation of hybrid cells in vivo （on the example of lupus erythematosus） depends on a contact of examinee with ecological toxicants.展开更多
文摘The kinetics of ecotoxicant transport through the membrane was studied. The ecotoxicants studied were pecticides widely used in agriculture: lontrel (clopyralid), sencor (metribuzin), basagran (bentazon), roundup (glyphosate), kusagard (alloxydim sodium), and sethoxydim, as well as lontrel complexes with cobalt and copper. All compounds considered penetrate through the model phosphatidylcholine liposomal membranes. The transfer rate was monitored by fluorescence quenching of ?-ATP inside the liposomes. The mathematical model for the process was proposed, and the mass transfer rates were calculated. The octanol/water partition constants were determined. All the compounds con- sidered were shown to accumulate in the fatty layer. The kinetics of their accumulation was studied, and the rates of accumulation in the nonpolar phase were calculated, which correlate with the complexation constants of the same compounds. Bioaccumulation of the toxicants is caused by the formation of complexes with the fatty phase (lipid part) of the cellular membranes. The toxicants under study transferred into the nonpolar phase with a considerable rate dur- ing the whole observation time, namely, 18 months.
文摘The analysis was made over the leukograms of leukocyte concentrate in venous blood of healthy people （77 persons） and a group consisting from 18 persons with hybrid cells （LE-phenomenon） detected in them. Authors constructed in vitro processes, which take place in vivo, and got the artificial morphological analogue of LE-cells through a physical and chemical agent--polyethylene glycol. The suggestion was made that the formation of hybrid cells in vivo （on the example of lupus erythematosus） depends on a contact of examinee with ecological toxicants.
