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Potential role of ecto-5'-nucleotidase in morphine-induced uridine release and neurobehavioral changes 被引量:1
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作者 CHE Xiao-hang LIU Ping +8 位作者 WU Chun-fu SONG Wu AN Ni-na YU Li-sha BAI Yi-jun XING Zheng CAI Jia-ling WANG Xiao-min YANG Jing-yu 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2018年第9期722-722,共1页
OBJECTIVE There is growing evidence that uridine may act as an endogenous neuromodulator with a potential signaling role in the central nervous system in addition to its function in pyrimidine metabolism.We previously... OBJECTIVE There is growing evidence that uridine may act as an endogenous neuromodulator with a potential signaling role in the central nervous system in addition to its function in pyrimidine metabolism.We previously found that acute morphine treatment significantly increased uridine release in the dorsal striatum of mice,while the mechanism involved in morphine-induced uridine release and the role of uridine in morphine-induced neurobehavioral changes have not been understood.METHODS Uridine release in the dorsal striatum of mice was assessed by in vivo microdialysis coupled with high performance liquid chromatography(HPLC) after morphine treatment.Western blotting and immunofluorescence were used to evaluate the expression of uridine-related proteins.Morphine-induced neurobehavioral changes were assessed by locomotor activity,behavioral sensitization and conditioned place preference(CPP)test.The expression of NT5E,an extracellular enzyme involved in formation of nucleosides,including uridine,was specifically knocked down in the dorsal striatum of mice using adeno-associated virus(AAV)-mediated short hairpin RNA(shRNA).RESULTS Both acute and chronic morphine administration significantly increased uridine release in the dorsal striatum,and this was associated with upregulation of NT5E but not other uridine-related proteins.Inhibition of NT5E with APCP or shRNA markedly inhibited morphine-induced uridine release in the dorsal striatum and related neurobehavioral changes,including hyperlocomotor activity,behavioral sensitization and CPP.CONCLUSION The present study increases our understanding of the contribution of NT5E in regulating morphine-induced neurobehavioral changes,at least as related to uridine,and suggests that NT5E may be a novel therapeutic target to manage morphine abuse. 展开更多
关键词 ecto-5'-nucleotidases URIDINE MORPHINE NEUROBEHAVIORAL CHANGES
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Study the effect of kidney stones on serum xanthine oxidase, ecto-5'-nucleotidase activity and E3 SUMO-protein ligase NSE2(NSMCE2) in Malaysian individuals
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作者 Faridah Yusof Atheer Awad Mehde +2 位作者 Wesen Adel Mehdi Hamid Ghazali Azlina Abd Rahman 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2015年第8期665-668,共4页
Objective: To verify possible relations between 5'-nucleotidase, xanthine oxidase to E3 small ubiquitin-like modifier-protein ligase non structural maintenance of chromosomes elements 2 in sera patients with kidne... Objective: To verify possible relations between 5'-nucleotidase, xanthine oxidase to E3 small ubiquitin-like modifier-protein ligase non structural maintenance of chromosomes elements 2 in sera patients with kidney stones and to evaluate the possibility of a new biomarker for the evaluation of kidney damage. Methods: A sixty patients with known kidney stones who appeared the government health clinics in Kuantan–Pahang and fifty apparently healthy were taken as control group. The 5'-nucleotidase,xanthine oxidase and other biochemical parameters were measured by colorimetric tests. The serum NSMCE2 were measured by enzyme linked immunosorbent assay.Results: The mean serum xanthine oxidase [(39.98±19.70) IU/L] and ecto-5'-nucleotidase activity(40.03±9.53 IU/L) were significantly higher than the controls' levels of(18.04 ±6.26) and(16.06 ±4.61) IU/L respectively. There were 85.00% and 83.33%, of patients with kidney stones who had abnormal ecto-5'-nucleotidase activity and uric acid respectively while xanthine oxidase activity was less sensitive 58.33%.Conclusions: The present study suggests that the increase in serum of xanthine oxidase,ecto-5'-nucleotidase activities E3 small ubiquitin-like modifier-protein ligase NSE2 concentration can be used as biomarkers for diagnosis of kidney damage in patients with kidney stone,also in developments of change DNA damage and inflammation disorders in these patients. 展开更多
关键词 Kidney stones XANTHINE OXIDASE ecto-5-nucleotidase E3 SUMO-protein LIGASE NSE2
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旋毛虫5′-nucleotidase基因特征与克隆表达 被引量:1
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作者 廖成水 王晓利 +7 位作者 杜付玉 郁川 余祖华 张春杰 李银聚 吴庭才 刘明远 程相朝 《中国人兽共患病学报》 CAS CSCD 北大核心 2018年第11期971-976,共6页
目的研究旋毛虫5′-nucleotidase基因的序列结构特征及其蛋白克隆表达。方法从旋毛虫获得5′-nucleotidase基因的序列,利用生物信息学方法进行系统性分析,并进行原核表达。结果旋毛虫5′-nucleotidase基因大小为1 653bp,编码550个氨基酸... 目的研究旋毛虫5′-nucleotidase基因的序列结构特征及其蛋白克隆表达。方法从旋毛虫获得5′-nucleotidase基因的序列,利用生物信息学方法进行系统性分析,并进行原核表达。结果旋毛虫5′-nucleotidase基因大小为1 653bp,编码550个氨基酸,119个氨基酸残基组成,理论相对分子质量(Mr)为62kD,分子式为C_(2800)H_(4358)N_(742)O_(803)S_(23),等电点为6.13,属于稳定存在的亲水蛋白质。该蛋白含有21个氨基酸组成的信号肽,同时具有跨膜区域,此外还含有3个N-糖基化位点、2个O-糖基化位点、20个磷酸化位点、28个B细胞线性结合位点和13个T细胞结合位点。二级结构中,α-螺旋占43.27%(238个),伸展链占22.73%(125个),β-折叠占7.82%(43个),无规则卷曲占26.18%(144个)。SDS-PAGE显示,5′-nucleotidase基因在原核表达系统中呈可溶性形式表达,重组蛋白大小约为74kD。结论成功克隆了旋毛虫5′-nucleotidase基因,对其进行序列分析和原核表达,为进一步探究5′-nucleotidase蛋白在旋毛虫感染过程中的作用奠定基础。 展开更多
关键词 旋毛虫 5-nucleotidase 克隆 原核表达 生物信息学
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Effect of Fertilizer Diammonium Phosphate on Liver,Kidney and Muscle 5-Nucleotidase Activity of Fresh Water Teleost Fish Clarias batrachus
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作者 T.S.NAQVI M.S.NAQVI R.K.SINGH 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 1993年第4期385-388,共4页
The toxic effect of fertilizer Diammonium phosphate resulted in alterations of 5'-Nucleotidase activity of tissues liver, kidney and muscles offish C. batrachus at varying intervals and exposures. Alterations in 5... The toxic effect of fertilizer Diammonium phosphate resulted in alterations of 5'-Nucleotidase activity of tissues liver, kidney and muscles offish C. batrachus at varying intervals and exposures. Alterations in 5'-Nuclcotidase activity of body organs gave an idea of the toxicity caused by the fertilizer. Thus the enzyme 5'-Nucleotidase can be used to monitor the pollution in aquatic ecosystem. 展开更多
关键词 Effect of Fertilizer Diammonium Phosphate on Liver Kidney and Muscle 5-nucleotidase Activity of Fresh Water Teleost Fish Clarias batrachus
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Prolonged intermittent theta burst stimulation restores the balance between A_(2A)R-and A_(1)R-mediated adenosine signaling in the 6-hydroxidopamine model of Parkinson's disease
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作者 Milica Zeljkovic Jovanovic Jelena Stanojevic +4 位作者 Ivana Stevanovic Milica Ninkovic Tihomir V.Ilic Nadezda Nedeljkovic Milorad Dragic 《Neural Regeneration Research》 SCIE CAS 2025年第7期2053-2067,共15页
An imbalance in adenosine-mediated signaling,particularly the increased A_(2A)R-mediated signaling,plays a role in the pathogenesis of Parkinson's disease.Existing therapeutic approaches fail to alter disease prog... An imbalance in adenosine-mediated signaling,particularly the increased A_(2A)R-mediated signaling,plays a role in the pathogenesis of Parkinson's disease.Existing therapeutic approaches fail to alter disease progression,demonstrating the need for novel approaches in PD.Repetitive transcranial magnetic stimulation is a non-invasive approach that has been shown to improve motor and non-motor symptoms of Parkinson's disease.However,the underlying mechanisms of the beneficial effects of repetitive transcranial magnetic stimulation remain unknown.The purpose of this study is to investigate the extent to which the beneficial effects of prolonged intermittent theta burst stimulation in the 6-hydroxydopamine model of experimental parkinsonism are based on modulation of adenosine-mediated signaling.Animals with unilateral 6-hydroxydopamine lesions underwent intermittent theta burst stimulation for 3 weeks and were tested for motor skills using the Rotarod test.Immunoblot,quantitative reverse transcription polymerase chain reaction,immunohistochemistry,and biochemical analysis of components of adenosine-mediated signaling were performed on the synaptosomal fraction of the lesioned caudate putamen.Prolonged intermittent theta burst stimulation improved motor symptoms in 6-hydroxydopamine-lesioned animals.A 6-hydroxydopamine lesion resulted in progressive loss of dopaminergic neurons in the caudate putamen.Treatment with intermittent theta burst stimulation began 7 days after the lesion,coinciding with the onset of motor symptoms.After treatment with prolonged intermittent theta burst stimulation,complete motor recovery was observed.This improvement was accompanied by downregulation of the e N/CD73-A_(2A)R pathway and a return to physiological levels of A_(1)R-adenosine deaminase 1 after 3 weeks of intermittent theta burst stimulation.Our results demonstrated that 6-hydroxydopamine-induced degeneration reduced the expression of A_(1)R and elevated the expression of A_(2A)R.Intermittent theta burst stimulation reversed these effects by restoring the abundances of A_(1)R and A_(2A)R to control levels.The shift in ARs expression likely restored the balance between dopamine-adenosine signaling,ultimately leading to the recovery of motor control. 展开更多
关键词 A_(1)R A_(2A)R adenosine receptors ADENOSINE ecto-5-nucleotidase intermittent theta burst stimulation non-invasive brain stimulation Parkinson's disease purinergic signalling
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Changes in Adenosine Metabolism in Asthma. A Study on Adenosine, 5&#39-NT, Adenosine Deaminase and Its Isoenzyme Levels in Serum, Lymphocytes and Erythrocytes
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作者 Jitender Sharma Bala K. Menon +1 位作者 Vannan K. Vijayan Surendra K. Bansal 《Open Journal of Respiratory Diseases》 2015年第2期33-49,共17页
Background: Adenosine deaminase (ADA) and 5'-nucleotidase (5'-NT) play a crucial role in adenosine metabolism in healthy individuals. Adenosine is an inflammatory mediator of asthma. Changes in adenosine metab... Background: Adenosine deaminase (ADA) and 5'-nucleotidase (5'-NT) play a crucial role in adenosine metabolism in healthy individuals. Adenosine is an inflammatory mediator of asthma. Changes in adenosine metabolism and role of ADA and 5'-NT in regulating adenosine level in asthmatics and correlation of these changes with severity of asthma are not clearly understood. Methods: In this study, we screened 5217 patients, of which 2416 were diagnosed with asthma. Further, of 2416 asthmatics, only 45 patients who strictly fulfilled the selection criteria were enrolled in the study. The patients were classified into mild, moderate and severe persistent groups;each group consisted of fifteen patients. Fifteen healthy subjects served as controls. Adenosine levels and activities of 5'-NT, total ADA, ADA1 and ADA2 in serum, lymphocytes and erythrocytes were determined. The data were analysed statistically and p vice versa. 展开更多
关键词 ASTHMA ADENOSINE METABOLISM ADENOSINE DEAMINASE 5'-nucleotidase
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鼠伤寒沙门菌5'-nucleotidase基因缺失株的构建及其生物学特性的研究 被引量:1
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作者 张梦珂 廖成水 +5 位作者 毛福超 王晓利 张春杰 李银聚 吴庭才 程相朝 《中国兽医科学》 CAS CSCD 北大核心 2019年第5期589-597,共9页
为了探究5'-nucleotidase基因的功能及其在鼠伤寒沙门菌感染中的作用,本研究首先构建缺失1 112 bp的5'-nucleotidase基因的重组自杀性质粒pR E 112Δ5'-nucleotidase,利用重组自杀性质粒介导的等位基因交换技术,两步法筛选... 为了探究5'-nucleotidase基因的功能及其在鼠伤寒沙门菌感染中的作用,本研究首先构建缺失1 112 bp的5'-nucleotidase基因的重组自杀性质粒pR E 112Δ5'-nucleotidase,利用重组自杀性质粒介导的等位基因交换技术,两步法筛选出SL1344Δ5'-nucleotidase缺失株,并对其生物学特性进行初步研究。PCR及测序结果表明,SL1344Δ5'-nucleotidase构建成功,且回复株SL1344CΔ5'-nucleotidase构建成功。进一步研究表明,缺失株SL1344Δ5'-nucleotidase保留了亲本菌株SL1344的血清型1,4,5,12∶i∶1,2,且能够稳定遗传缺失1 112 bp的5'-nucleotidase基因,生长速度没有发生明显改变。但是,缺失株SL1344Δ5'-nucleotidase口服感染6周龄BALB/c小鼠的LD50为4.30×10~7CF U,毒力降低至亲本株SL1344的1.96%。免疫保护率试验结果显示,对6周龄BALB/c小鼠免疫后第17天,鼠伤寒沙门菌野生株攻毒有50%的保护率。缺失株免疫小鼠后第21天,血清抗体IgG水平达到最高。回复菌株与亲本亲株之间生物学特性无显著性差异,基本恢复到野生型水平。上述结果表明,SL1344Δ5'-nucleotidase基因缺失株构建成功,遗传稳定,毒力明显降低,且具有较好的免疫原性,为研究鼠伤寒沙门菌5'-nucleotidase基因与其致病力之间的关系提供了依据,并为研究它在鼠伤寒沙门菌感染中的作用奠定了基础。 展开更多
关键词 鼠伤寒沙门菌SL1344 5-nucleotidase基因 缺失株 回复株 生物学特性
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淋巴管生成因子与大肠癌转移的相关性研究 被引量:1
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作者 吕媛芳 贺建宇 《临床肿瘤学杂志》 CAS 2009年第4期309-313,共5页
目的:探讨淋巴管生成因子的定量表达与大肠癌转移的相关性。方法:本文通过收集已有淋巴结转移的大肠腺癌手术标本37例,运用荧光实时定量PCR技术对淋巴管生成因子LYVE-1、Prox-1、podoplanin及5’-nucleotidase分子表达进行定量分析,分... 目的:探讨淋巴管生成因子的定量表达与大肠癌转移的相关性。方法:本文通过收集已有淋巴结转移的大肠腺癌手术标本37例,运用荧光实时定量PCR技术对淋巴管生成因子LYVE-1、Prox-1、podoplanin及5’-nucleotidase分子表达进行定量分析,分别比较这些因子在切除标本的肿瘤、癌旁正常组织中表达的差异。结果:Prox-1及podoplanin在肿瘤及癌旁正常组织分别为:Prox-1表达量为0.96±0.25和0.52±0.22(P=0.003 8),podoplanin表达量为0.87±0.26和0.78±0.22(P=0.028 8),并与肿瘤的淋巴结转移有关。LYVE-1在肿瘤及癌旁正常组织中表达都明显增加,分别为0.90±0.28和0.87±0.26(P=0.365),未检出5’-nucleotidase在肿瘤及癌旁正常组织中的表达。结论:Prox-1及podoplanin与大肠癌发生及淋巴结转移可能有一定关系,联合检测Prox-1和podoplanin的表达,对了解大肠癌的侵袭转移及判定预后具有一定价值。 展开更多
关键词 大肠癌 淋巴管内皮标记物 PROX-1 PODOPLANIN 5-nucleotidase LYVE-1
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The Sources of Extracellular Adenosine in Hippocampus and Neostriatum of the Rat Brain
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作者 M.A.Pak Y.Yetkin 《World Journal of Neuroscience》 2014年第2期85-91,共7页
Endogenous adenosine can enter the extracellular space either by direct release or via formation from adenine nucleotides. We have compared the effects of substances blocking the ecto-5'-nucleotidase with a, b-met... Endogenous adenosine can enter the extracellular space either by direct release or via formation from adenine nucleotides. We have compared the effects of substances blocking the ecto-5'-nucleotidase with a, b-methylene adenosine 5'-diphosphate (AOPCP) or adenosine kinase with iodotubercidin (Itu) on field potentials in the hippocampus and the neostriatum in vitro evoked by stimulation of the stratum radiatum or the cortico-striatal pathway respectively. AOPCP enhanced the amplitude of the population spikes by 34% in the hippocampus and by 26.5% in the neostriatum. DPCPX, a selective A1-receptor antagonist, increased the amplitude of the population spikes by 68% in the hippocampus and by 53.5% in the neostriatum. Thus both, release of adenosine from the intracellular space and, extracellular dephosphorylation of adenine nucleotides to a lesser extent, contribute to the effective levels of adenosine in the extracellular space in hippocampus and neostriatum. 展开更多
关键词 AOPCP ecto-5'-nucleotidase Iodotubercidin DPCPX ELECTROPHYSIOLOGY
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The Biochemical and Cytochemical Study of 5'-nucleotldase Activity in Chilling-sensitive and Chilling-tolerant Rice Plasmalemma
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作者 潘杰 孙龙华 简令成 《Chinese Science Bulletin》 SCIE EI CAS 1993年第1期81-85,共5页
5′-nucleotidase (EC 3.1.3.5) can catalyze the hydrolysis of most ribonucleoside 5′-monophosphates and deoxynucleoside 5′-monophosphates to the corresponding nucleosides and orthophosphates. It is localized predomin... 5′-nucleotidase (EC 3.1.3.5) can catalyze the hydrolysis of most ribonucleoside 5′-monophosphates and deoxynucleoside 5′-monophosphates to the corresponding nucleosides and orthophosphates. It is localized predominantly in the plasma membranes of human and animal tissues,and often used as a marker enzyme for plasma membranes 展开更多
关键词 5-nucleotidase RICE chilling-tolerance.
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Skewed CD39/CD73/adenosine pathway contributes to B-cell hyperactivation and disease progression in patients with chronic hepatitis B 被引量:1
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作者 Shuang-Nan Zhou Ning Zhang +8 位作者 Hong-Hong Liu Peng Xia Chao Zhang Jin-Wen Song Xing Fan Ming Shi Lei Jin Ji-Yuan Zhang Fu-Sheng Wang 《Gastroenterology Report》 SCIE EI 2021年第1期49-58,I0002,共11页
Background:The mechanisms underlying B-cell hyperactivation in patients with chronic hepatitis B virus(HBV)infection remain largely undefined.The present study assessed the clinical characteristics of the CD39/CD73/ad... Background:The mechanisms underlying B-cell hyperactivation in patients with chronic hepatitis B virus(HBV)infection remain largely undefined.The present study assessed the clinical characteristics of the CD39/CD73/adenosine pathway in patients with chronic hepatitis B(CHB).Methods:We examined CD39 and CD73 expression and adenosine production by B-cells from 202 HBV-infected patients.B-cell-activation phenotypes were assessed by flow cytometry after CpG+CD40 ligand stimulation with or without blockade and activation of the adenosine pathway.Results:CD39 and CD73 expression on circulating B-cells was decreased in CHB patients with high HBV DNA,HBeAg positivity,high HBsAg levels,and active liver inflammation,and was hierarchically restored in complete responders according to HBeAg seroconversion or HBsAg reduction.However,CD39 and CD73 expression on activated memory and tissue-like memory B-cell subsets in complete responders was not increased despite effective antiviral treatments.Furthermore,CD39 and CD73 expression on intra-hepatic B-cells was decreased in inflammatory livers.In vitro,B-cells from CHB patients showed a markedly reduced capacity to generate CD39/CD73-dependent extracellular adenosine and expressed increased levels of activation markers after adenosine-production blockade.Contrastingly,metformin significantly reduced activation-marker expression via regulating AMP-activated protein kinase.Conclusions:The skewed CD39 and CD73 expression on B-cells was associated with a high viral burden,liver inflammation,and antiviral efficacy in CHB patients,and the skewed CD39/CD73/adenosine pathway contributed to B-cell hyperactivation.Regulation of the CD39/CD73/adenosine pathway using metformin may represent a therapeutic option to reverse HBVinduced immune pathogenesis. 展开更多
关键词 hepatitis B virus B-LYMPHOCYTE CD39 5-nucleotidase lymphocyte activation
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