Objective:To investigate the mechanistic basis for the attenuation of bone degeneration by edible bird's nest(EBN)in ovariectomized rats.Methods:Forty-two female Sprage-Dawley rats were randomized into 7 groups(6 ...Objective:To investigate the mechanistic basis for the attenuation of bone degeneration by edible bird's nest(EBN)in ovariectomized rats.Methods:Forty-two female Sprage-Dawley rats were randomized into 7 groups(6 in each group).The ovariectomized(OVX)and OVX+6%,3%,and 1.5%EBN and OVX+estrogen groups were given standard rat chow alone,standard rat chow+6%,3%,and 1.5%EBN,or standard rat chow+estrogen therapy(0.2mg/kg per day),respectively.The sham-operation group was surgically opened without removing the ovaries.The control group did not have any surgical intervention.After 12 weeks of intervention,blood samples were taken for serum estrogen,osteocalcin,and osteoprotegerin,as well as the measurement of magnesium,calcium abd zinc concentrations.While femurs were removed from the surrounding muscles to measure bone mass density using the X-ray edge detection technique,then collected for histology and estrogen receptor(ER)immunohistochemistry.Results:Ovariectomy altered serum estrogen levels resulting in increased food intake and weight gain,while estrogen and EBN supplementation attenuated these changes.Ovariectomy also reduced bone ER expression and density,and the production of osteopcalcin and osteorotegerin,which are important pro-osteoplastic hormones that promote bone mineraliztion and density.Conversely,estrogen and EBN increased serum estrogen levels leading to increased bone ER expression,pro-osteoplastic hormone production and bone density(all P<0.05).Conclusion:EBN could be used as a safe alternative to hormone replacement therapys for managing menopausal complications like bone degeneration.展开更多
测定燕窝中蛋白质、氨基酸、脂肪和唾液酸的含量,以了解燕窝的营养成分;运用正交试验研究木瓜蛋白酶酶解提取燕窝唾液酸的最佳工艺条件.结果表明:燕窝是一种高蛋白、低脂肪的食品,必需氨基酸占氨基酸总量的42%,特征活性物质——唾液酸...测定燕窝中蛋白质、氨基酸、脂肪和唾液酸的含量,以了解燕窝的营养成分;运用正交试验研究木瓜蛋白酶酶解提取燕窝唾液酸的最佳工艺条件.结果表明:燕窝是一种高蛋白、低脂肪的食品,必需氨基酸占氨基酸总量的42%,特征活性物质——唾液酸的含量达13.47%;在酶解时间2.5 h、液料比30∶1、酶解温度45℃、p H 8的最佳提取工艺条件下,唾液酸的提取率为(9.98±0.05)%,比传统水提法的提取率提高了9.91%.展开更多
基金Supported by Grants from Ministry of Science,Technology and Innovation,E-science Fund(No.5450666)Malaysia+3 种基金the Health and Family Planning Commission of Hebei(No.20160315)the Administration of Traditional Chinese Medicine of Hebei(No.2017196)the Key Discipline Construction Project of Hebei Provincial Universities[No.JiJiao Gao-2013-(4)-2012-37]Start up Fund for High Level Talents of Chengde Medical University(No.201704)。
文摘Objective:To investigate the mechanistic basis for the attenuation of bone degeneration by edible bird's nest(EBN)in ovariectomized rats.Methods:Forty-two female Sprage-Dawley rats were randomized into 7 groups(6 in each group).The ovariectomized(OVX)and OVX+6%,3%,and 1.5%EBN and OVX+estrogen groups were given standard rat chow alone,standard rat chow+6%,3%,and 1.5%EBN,or standard rat chow+estrogen therapy(0.2mg/kg per day),respectively.The sham-operation group was surgically opened without removing the ovaries.The control group did not have any surgical intervention.After 12 weeks of intervention,blood samples were taken for serum estrogen,osteocalcin,and osteoprotegerin,as well as the measurement of magnesium,calcium abd zinc concentrations.While femurs were removed from the surrounding muscles to measure bone mass density using the X-ray edge detection technique,then collected for histology and estrogen receptor(ER)immunohistochemistry.Results:Ovariectomy altered serum estrogen levels resulting in increased food intake and weight gain,while estrogen and EBN supplementation attenuated these changes.Ovariectomy also reduced bone ER expression and density,and the production of osteopcalcin and osteorotegerin,which are important pro-osteoplastic hormones that promote bone mineraliztion and density.Conversely,estrogen and EBN increased serum estrogen levels leading to increased bone ER expression,pro-osteoplastic hormone production and bone density(all P<0.05).Conclusion:EBN could be used as a safe alternative to hormone replacement therapys for managing menopausal complications like bone degeneration.
文摘测定燕窝中蛋白质、氨基酸、脂肪和唾液酸的含量,以了解燕窝的营养成分;运用正交试验研究木瓜蛋白酶酶解提取燕窝唾液酸的最佳工艺条件.结果表明:燕窝是一种高蛋白、低脂肪的食品,必需氨基酸占氨基酸总量的42%,特征活性物质——唾液酸的含量达13.47%;在酶解时间2.5 h、液料比30∶1、酶解温度45℃、p H 8的最佳提取工艺条件下,唾液酸的提取率为(9.98±0.05)%,比传统水提法的提取率提高了9.91%.
