Previous studies have shown that baicalin prevented iron accumulation after substantia nigra injury, reduced divalent metal transporter 1 expression, and increased ferroportin 1 expression in the substantia nigra of r...Previous studies have shown that baicalin prevented iron accumulation after substantia nigra injury, reduced divalent metal transporter 1 expression, and increased ferroportin 1 expression in the substantia nigra of rotenone-induced Parkinson＇s disease rats. In the current study, we investigated the relationship between iron accumulation and transferrin expression in C6 cells, to explore the mechanisms of the inhibitory effect of baicalin on iron accumulation observed in Parkinson＇s disease rats. Iron content was detected using inductively coupled plasma-atomic emission spectroscopy. Results showed that iron content decreased 41% after blocking divalent metal transporter 1 and ferroportin 1 proteins. After treatment with ferric ammonium citrate of differing concentrations （10, 50, 100, 400 ktg/mL） in C6 glioma cells, cell survival rate and ferroportin 1 expression were negatively correlated with ferric ammonium citrate concentration, but divalent metal transporter 1 expression positively correlated with ferric ammonium citrate concentration. Baicalin or deferoxamine reduced divalent metal transporter 1 expression, but increased ferroportin 1 expression in the 100 μg/mL ferric ammonium citrate-loaded C6 cells. These results indicate that baicalin down-regulated iron concentration, which positively regulat- ed divalent metal transporter 1 expression and negatively regulated ferroportin 1 expression, and decreased iron accumulation in the substantia nigra.展开更多
Based on the theory of collaborative innovation and the triple helix theory combined with the needs for innovative development in the modern manufacturing industry in Guangdong Province,the construction of open bases ...Based on the theory of collaborative innovation and the triple helix theory combined with the needs for innovative development in the modern manufacturing industry in Guangdong Province,the construction of open bases as education carriers by the governments was explored in terms of implement joint training for postgraduates in engineering through the integration of science-education and industry-education,to improve the practical innovation ability of postgraduates in engineering.The integration training mode has significantly improved the effectiveness of collaborative education and innovation,and it has become an important talent and intellectual support for Guangdong’s modern manufacturing industry.It has further advanced the theory and practice of joint training conducted by governments,industries,universities,and scientific research institutes.展开更多
基金supported by the Scientific Research Common Program of Beijing Municipal Commission of Education,No.KM20110025010
文摘Previous studies have shown that baicalin prevented iron accumulation after substantia nigra injury, reduced divalent metal transporter 1 expression, and increased ferroportin 1 expression in the substantia nigra of rotenone-induced Parkinson＇s disease rats. In the current study, we investigated the relationship between iron accumulation and transferrin expression in C6 cells, to explore the mechanisms of the inhibitory effect of baicalin on iron accumulation observed in Parkinson＇s disease rats. Iron content was detected using inductively coupled plasma-atomic emission spectroscopy. Results showed that iron content decreased 41% after blocking divalent metal transporter 1 and ferroportin 1 proteins. After treatment with ferric ammonium citrate of differing concentrations （10, 50, 100, 400 ktg/mL） in C6 glioma cells, cell survival rate and ferroportin 1 expression were negatively correlated with ferric ammonium citrate concentration, but divalent metal transporter 1 expression positively correlated with ferric ammonium citrate concentration. Baicalin or deferoxamine reduced divalent metal transporter 1 expression, but increased ferroportin 1 expression in the 100 μg/mL ferric ammonium citrate-loaded C6 cells. These results indicate that baicalin down-regulated iron concentration, which positively regulat- ed divalent metal transporter 1 expression and negatively regulated ferroportin 1 expression, and decreased iron accumulation in the substantia nigra.
基金The Guangdong Provincial Postgraduate Education Innovation Program and the Guangdong Provincial Philosophy and Social Science Planning 2022“Talent Research Special,”“Research on the Institutional System of High-quality Engineering Master’s Training Bases in the Deep Integration of Production and Education”(Project Number:2021JGXM103)。
文摘Based on the theory of collaborative innovation and the triple helix theory combined with the needs for innovative development in the modern manufacturing industry in Guangdong Province,the construction of open bases as education carriers by the governments was explored in terms of implement joint training for postgraduates in engineering through the integration of science-education and industry-education,to improve the practical innovation ability of postgraduates in engineering.The integration training mode has significantly improved the effectiveness of collaborative education and innovation,and it has become an important talent and intellectual support for Guangdong’s modern manufacturing industry.It has further advanced the theory and practice of joint training conducted by governments,industries,universities,and scientific research institutes.
