Biomechanical Experimental Study on Effective Fraction of Radix Salviae Miltiorrhizae on Healing of Bone Fracture

Objective: To assess the effect of Danshen 9403 (DS 9403), an effective fraction of Radix Salviae miltiorrhizae (RSM) on healing of bone fracture. Methods: Standardized radial fracture was performed in 120 Wistar rats. The model rats were randomized into four groups: Group A was fed with DS 9403, group B injected with Staphylococcus aureus , group C with normal saline administration, and group D with RSM injection. The treatment began at the first day of fracture. The rats were sacrificed on the day 25, 39 and 50 separately in batches and their intact radii were removed by dissection for detecting load and stress of three point bending test with autograph universal material testing machine (Shimazu, Japan). Results: The parameters of load in DS 9403 treated group on the 39th day and that of stress at 25th, 39th and 50th day were (6.20±1.32)N, (5.71±3.58)N/mm 2, (8.27±2.42)N/mm 2 and (66.25±26.21)N/mm 2 respectively, which were significantly higher than those in other groups, P <0.05. Conclusion: DS 9403 has the action of increasing the strength of fracture broken end.

Protective effect of ultrashortwave versus radix salviae miltiorrhizae on brains of rats with cerebral ischemia-reperfusion injury

BACKGROUND： HOW to control the effect of oxygen-derived free radicals on development of cerebral injury and cerebral edema is a key factor for treating cerebral ischemia-reperfusion injury. OBJECTIVE： To observe and compare the protective effects, synergistic action and mechanisms of ultrashortwave （USW） and radix salviae miltiorrhizae （RSM） on the focal cerebral ischemia-reperfusion injuries in rats. DESIGN： Randomized controlled animal study SEI-FING： Department of Rehabilitation Medicine, First Hospital affiliated to China Medical University MATERIALS： A total of 160 healthy Wistar rats of both genders and aged 18-20 weeks weighing 250-300 g of clean grade were selected in this study. 5 mL/ampoule RSM injection fluid was produced by the First Pharmaceutical Corporation of Shanghai （batch number： 011019, 0.01 mug）. The USW therapeutic device was produced by Shanghai Electronic Device Factory with the frequency of 40.68 MHz and the maximal export power of 40 W. The first channel of power after modulation was 11 W. METHODS： The experiment was carried out in the Rehabilitation Medicine Department of the First Hospital affiliated to China Medical University from May 2002 to January 2003. Focal ischemia-reperfusion model was established in rats by reversible right middle cerebral artery occlusion with filament. Right cerebral ischemia was for 2 hours and then with 24 hours reperfusion. The scores of neurological deficits were evaluated by 0 to 4 scales. After surgery, 64 successful rats models were divided into four groups according to digital table： control group, USW group, RSM group and RSM ＋ USW group with 16 cases in each group. Rats in control group were intraperitoneally injected with the same volume of saline （0.1 mL/g）; rats in USW group were given small dosage of USW on head for 10 minutes at 6 hours after reperfusion; rats in RSM group were intraperitoneally injected with 0.01 mL/g RSM solution at 30 minutes before reperfusion; rats in RSM ＋ USW group were intraperitoneally injected with 0.01 mL/g RSM parenteral solution at 30 minutes before reperfusion and given small dosage of USW on head for 10 minutes once at 6 hours after reperfusion; sixteen rats in sham operation group did not receive any treatment. All 80 rats were taken brains at 24 hours after reperfusion to measure wet and dry weights to calculate water content： Cerebral water content （%） = （1-dry/wet weight） × 100%. Superoxide dismutase （SOD） activity was measured by hydroxylamine method and malondialdehyde （MDA） content was measured by TBA photometric method. MAIN OUTCOME MEASURES ： Cerebral water content, SOD activity and MDA content RESULTS： All 160 rats except 80 failing in modeling were involved in the final analysis. （① The cerebral water content of left hemisphere made no significant difference （P 〉 0.05）. The cerebral water content of right hemisphere in the control group and the three treatment groups was obviously higher than that of the sham operation group [（81.26±0.77）%, （79.74±0.68）%, （79.76±0.81）%, （79.61±0.79）%, （77.43±0.61）%, P 〈 0.05]. The cerebral water content of right hemisphere in the three treatment groups was obviously lower than that of the control group （P〈 0.05）. There was no significant difference among the three treatment groups （P 〉 0.05）. ② Compared with the control group, SOD activity （right） of the control group decreased obviously （P 〈 0.05）, while MDA content increased obviously （P 〈 0.05）. SOD activity in the three therapeutic groups increased obviously, while MDA content decreased obviously （P 〈 0.05）; there was no significant difference among the three treatment groups （P 〉 0.05）. CONCLUSION： ① USW and RSM therapy have neuroprotective effects against focal cerebral ischemia-reperfusion injuries by means of decreasing cerebral water content and MDA and increasing the activity of SOD. ② Synergistic action was not observed between these two therapeutic methods.

EFFECTS OF HYPOXIC ENDOTHELIAL CELL CONDITIONED MEDIUM ON PROLIFERATION AND COLLAGEN SYNTHESIS OF SMOOTH MUSCLE CELLS AND INHIBITORY EFFECTS OF RADIX SALVIAE MILTIORRHIZAE

The effects of hypoxic endothelial cell conditioned medium (HECCM) on proliferation and collagen synthesis of cultured porcine pulmonary arterial smooth muscle cells (PASMCs) were studied by 3H-thymidine (3H-TdR) and 3H-proline incorporations, image analysis for determination of DNA content and colorimetric assay using MTT, and the inhibitory effects of radix salviae miltiorrhizae (RSM) on them were also investigated. The results showed that HECCM could induce enhancement of the enzymatic activity of mitochondria, increase of the nucleic DNA content and increases of the 3H-TdR and 3H-proline incorporations in PASMCs. The 3H-proline incorporation in PASMCs cultured in HECCM was 1.83 times as much as that cultured in normoxic endothelial cell conditioned medium (NECCM). Compared with the control, Chinese herb medicine RSM could inhibit the proliferation of PASMCs cultured in HECCM and decrease the 3H-prolinc incorporation in PASMCs cultured in both HECCM and NECCM (P< 0.001). However, RSM had no ef fects on the nucleic DNA content and 3H-TdR incorporation into DNA of PASMCs cultured in NECCM. It suggests that hypoxia may stimulate the endothelia to synthesize and secrete some cytokines which can stimulate the proliferation and the synthesis of collagen of PASMCs and RSM can inhibit this process.

Effects of Tetramethylpyrazine and Radix Salviae Miltiorrhizae on Collagen Synthesis and Proliferation of Cardiac Fibroblasts

Objective: To explore the effects of Tetramethylpyrazine (TMP) and Radix Salviae Miltiorrhizae (RSM) on collagen synthesis and proliferation of cardiac fibroblasts. Methods: Using collagenase and pancreatin digested rat cardiac tissue assay to isolate cardiac fibroblasts (FB). Different dosage of TMP, RSM and norepinephrine were used to study their effects on the collagen synthesis and proliferation of cultured cardiac FB. Results: Compared with the control group, moderate or high dosage TMP and RSM could significantly inhibit the collagen synthesis and the proliferation of cultured cardiac FB. Moreover, low-dose TMP (50 mg/L) and low-dose RSM (3 g/L) could antagonize the collagen synthesis and the proliferation of cultured cardiac FB stimulated by NE (500μg/L). Conclusion: Both TMP and RSM can inhibit the collagen synthesis and proliferation of cultured cardiac FB processes.The mechanisms of these effects might be correlated to their Ca++ antagonistic action. Original article on CJIM(Chin) 1998; 18(7): 423

Effects of Radix Salviae Miltiorrhizae on Proliferation,Apoptosis and c-myc Protein Expression of Fibroblast in Culture of Kindney with Lupus Nephritis

Objectiv:To observe the effects ofRadix Salviae Miltiorrhizae (SM) on humanfibroblast in culture of kidney with lupus nephritis (LN ). Methods: Fibroblasts wereisolated from culture of kidney biopsy of LN patients, and effect of SM on 3H-TdR incorporated rate of fibroblasts was observed. Theapoptosis and c-myc expression were detectedin the same time by flow cytometry.Results:SM could inhibit the proliferation of fibrolast,and promote the programmed cell deaththrough upregulate c-myc protein expression inhuman renal fibroblasts. Conclusions: Longterm administration of SM in large dosagecould be effective on interstial fibrosis of LN,so that to prevent or reduce the scar tissue for-mation and teatrd the occurrence of uremia.

Study on Preventive and Therapeutic Effect of Radix Salviae Miltiorrhizae on Recurrence and Metastasis of Liver Cancer

Objective: To study the inhibitory effect of Radix Salviae Miltiorrhizae (RSM) on adhesive and invasive ability of SMMC 7721 liver cancer cells, and on the metastasis and recurrence on post hepatectomy liver cancer in nude mice. Methods: Effect of RSM on SMMC 7721 cell membrane intercellular adhesive molecule 1 (ICAM 1) expression was observed by immunofluorescence flow cytometry; effect on invasive ability and of SMMC 7721 cell and the detachment of which attached to fibronectin (FN) by cell migration experiment; effect on adhesion of SMMC 7721 cell with FN by MTT method and effect on adhesion between 7721 7721, 7721 lymphocyte and 7721 endothelial cell by cell adhesion experiment. LCI D20 human liver cancer metastasis model, after hepatectomy, was used to observe the effect of RSM on recurrence and metastasis of liver carcinoma in nude mice. Results: ICAM 1 expression in SMMC 7721 cells incubated with RSM was significantly lower than that in cells did not treated with RSM. RSM could inhibit the invasive ability of SMMC 7721 cell and made the cells already attached to FN exfoliated. It could also inhibit the adhesion of 7721 7721, 7721 lymphocyte and 7721 endothelial cell. And RSM showed preventive and therapeutic effect on intrahepatic and remote metastasis/recurrence of early and late human post hepatectomy liver cancer in nude mice. Conclusion: RSM could inhibit the invasion and adhesion of SMMC 7721 cell and could also prevent and inhibit metastasis and recurrence of human liver cancer after hepatectomy in nude mice.

基于本草考证的丹参功效研究

丹参用药历史悠久,《神农本草经》中即有相关记载。2020版《中华人民共和国药典》载丹参功效为活血祛瘀、通经止痛、清心除烦、凉血消痈。但历代医家对丹参的功效理解认识不一,甚至有相悖的现象。本研究分别从汉晋南北朝时期、唐宋金元时期、明清时期和近代时期,通过本草考证对历代本草进行深入挖掘与梳理。经过系统考证,梳理了历代主流本草中丹参功效认识的沿革。发现历代本草中所记录的正品丹参即为历版《中华人民共和国药典》所载的丹参品种,主要功效为活血化瘀。

丹参有效部位对骨折愈合过程中胶原基因表达的影响

观察丹参有效部位（ＲＳ９４０３）对骨折愈合过程中前胶原、转化生长因子β１（ＴＧＦβ１）基因表达模式的影响，分析ＲＳ９４０３促进骨折愈合作用的分子机理。方法：健康成年Ｗｉｓｔａｒ大鼠２４只，作双侧桡骨骨折模型，随机分成ＲＳ９４０３组、阳性对照药三花接骨散组（三花组）和空白对照组（空白组），分别以ＲＳ９４０３、三花接骨散和生理盐水灌胃给药，术后３天、 １周、 ２周和 ４周后处死大鼠取材，采用不脱钙的大鼠骨痂组织冰冻切片进行原位杂交，观察骨痂组织中前胶原和 ＴＧＦβ１基因的表达。结果：骨折后第 ３天，ＲＳ９４０３组与三花组的骨折端 ＴＧＦβ１ ｍＲＮＡ的表达明显高子空白组，并已出现血型胶原基因的表达。骨折第１周末，成纤维细胞和软骨细胞样细胞的Ⅲ型前胶原基因表达占主导，ＲＳ９４０３组与三花组Ⅱ、Ⅰ型前胶原与 ＴＧＦβ１基因的表达较空白组里同步增强趋势。骨折第 ２周末， Ⅰ型前胶原 ｍＲＮＡ表达明显增加，ＲＳ９４０３组和三花组中肥大软骨细胞明显多于空白组，Ⅱ型前胶原ｍＲＮＡ的表达呈现显著的衰落趋势。同时证实共有表型表达现象的存在，尤以ＲＳ９４０３组与三花组更为明显。骨折第４周末，软骨骨痂基本被骨组织替代。 ＲＳ９４０３组和三花组?

丹参有效部位(丹参-9403)对骨折愈合影响的生物力学实验研究

目的：探讨丹参提取的有效部位（丹参－９４０３）对骨折愈合的影响。方法：用Ｗｉｓｔａｒ大白鼠进行标准骨折（桡骨中１／３）造模后，随机分为４组（即丹参－９４０３、金葡液、生理盐水及丹参注射液组），术后第２天开始分别灌胃各组药物，连续喂养２５、３９、５０天后处死，取完整桡骨，置于岛津Ａｕｔｏｇｒａｐｈ万能材料测试仪上测定３点抗折强度的载荷、应力。结果：丹参－９４０３组在３９天的载荷指标和２５、３９、５０天应力指标最高，分别为（６２０±１３２）牛顿（Ｎ），（５７１±３５８）Ｎ／ｍｍ２、（８２７±２４２）Ｎ／ｍｍ２、（６６２５±２６５１）Ｎ／ｍｍ２，与其他各组比较，有显著性差异（Ｐ＜００５）。结论：丹参－９４０３具有提高骨折断端强度的作用。

丹参 甘露醇对肢体缺血再灌注所致远处器官损伤的保护作用

目的 通过动物实验观察丹参和甘露醇对肢体缺血再灌注所致心、肝、肾、胰损伤的保护作用。方法 15 0只Wistar大鼠随机分成正常对照组、缺血 2h组、缺血 3h组、缺血 3h组用丹参组和缺血 3h用甘露醇组 5个组。实验组于缺血、再灌注 1、2、3和 2 4h取材光镜对比观察。结果 肢体缺血和再灌注后 ,实验组心、肝、肾、胰毛细血管扩张、充血、中性白细胞浸润、细胞变性、组织损伤。用丹参和甘露醇组的病变明显轻于未用药组。

肢体缺血再灌注致肺损伤及丹参的保护作用

目的 通过动物实验方法观察丹参对肢体缺血再灌注所致肺损伤的保护作用。方法 110只Wistar大鼠随机分成正常对照、缺血 3h和缺血 3h用丹参三个组。实验组于缺血、再灌注 1、2、3和 2 4h取材 ,光镜下对比观察和组织学评估。结果 肢体缺血和再灌注后 ,实验组肺泡隔增厚、水肿 ,毛细血管扩张、充血 ,大量多核白细胞浸润、贴壁 ,部分肺不张。用丹参组的病理改变明显轻于未用丹参组。

丹参现代研究概况与进展(续一)

近年来,对丹参的生物学特点、化学成分、药理活性、作用机制、临床制剂和临床用途等方面的研究均取得了较大进展。研究结果表明,不同产地的丹参含有的化学成分有明显的差异,是造成其临床效果不一的主要原因。丹参含有多种化学成分,可以分为丹参酮和丹酚酸两大类化合物。丹参酮类化合物多数有二萜结构,目前分离鉴定的化合物有30余种;丹酚酸类化合物多具有酚酸结构,结构明确的丹酚酸有20余种,这些化合物构成了丹参的有效成分,是丹参发挥治疗作用的物质基础。丹参有效成分的主要药理作用表现在多个方面,丹参酮类化合物以改善血液循环、抗茵和抗炎作用为主;而丹酚酸类化合物则以抗氧化、抗凝血和细胞保护作用特别突出。利用丹参已经研究成功多种临床应用的药物,包括注射剂、口服制剂等20余种,分别用于治疗心血管系统疾病、皮肤病、肝肾疾病等,并取得良好效果。

丹参现代研究概况与进展(续前)

近年来 ,对丹参的生物学特点、化学成分、药理活性、作用机制、临床制剂和临床用途等方面的研究均取得了较大进展。研究结果表明 ,不同产地的丹参含有的化学成分有明显的差异 ,是造成其临床效果不一的主要原因。丹参含有多种化学成分 ,可以分为丹参酮和丹酚酸两大类化合物。丹参酮类化合物多数有二萜结构 ,目前分离鉴定的化合物有 3 0余种 ;丹酚酸类化合物多具有酚酸结构 ,结构明确的丹酚酸有 2 0余种 ,这些化合物构成了丹参的有效成分 ,是丹参发挥治疗作用的物质基础。丹参有效成分的主要药理作用表现在多个方面 ,丹参酮类化合物以改善血液循环、抗菌和抗炎作用为主 ;而丹酚酸类化合物则以抗氧化、抗凝血和细胞保护作用特别突出。利用丹参已经研究成功多种临床应用的药物 ,包括注射剂、口服制剂等 2 0余种 ,分别用于治疗心血管系统疾病、皮肤病、肝肾疾病等 。

丹参药物血清对病理条件下VEC血管调节物质和自由基的调控作用

目的探讨丹参药物血清对血管内皮细胞分泌ET、SOD和MDA的影响及其治疗血瘀证的机理。方法经LPS和丹参药物血清作用于人脐静脉内皮细胞后,取上清培养液测定ET、NO、SOD和MDA水平。结果丹参药物血清可缓解LPS所致NO与ET的水平升高,并改善LPS所致SOD的活性降低和MDA的水平升高。结论丹参可以维持NO与ET的动态平衡,去除自由基,维持血管正常生理功能,从而能有效防治血瘀证。

超短波与丹参对脑缺血再灌注损伤大鼠脑保护作用的比较研究

目的 :观察并比较超短波与丹参对大鼠局灶性脑缺血再灌注损伤的保护作用并探讨两者作用机制和有无协同作用。方法 :用线栓法制备一侧大脑中动脉栓塞 再灌注大鼠模型 ,造成大鼠右脑缺血 2h再灌注 2 4h ,采用 5级评分法评定神经功能缺损程度来筛选病例。造模后 ,大鼠分成假手术组 ,对照组 ,超短波组 ,丹参组及超短波与丹参合用组。所有大鼠均于再灌注 2 4h后断头取脑 ,分别观察脑含水量、缺血侧脑组织的超氧化物歧化酶(SOD)和丙二醛 (MDA)含量。结果 :超短波治疗、丹参治疗及二者合用治疗均能减轻大鼠缺血侧脑含水量 (P <0 .0 5 ) ,提高抗氧化酶SOD含量 (P <0 .0 5 ) ,降低自由基产物MDA的含量 (P <0 .0 5 ) ,3个治疗组之间差异无显著性意义。结论 :超短波治疗与丹参治疗对大鼠局灶性脑缺血再灌注损伤具有神经保护作用 ,此作用可能与减轻脑水肿 ,升高SOD ,降低MDA有关 ,二者疗效比较未见明显差异。

丹参加玻璃酸钠对兔膝关节软骨保护的效应与机制研究

目的通过观察丹参加玻璃酸钠关节内注射,探讨其对兔膝关节软骨的保护效应与机制。方法通过切断膝关节前交叉韧带和内侧副韧带造成膝OA模型,将80只日本大耳白兔随机分为A组、B组、C组、D组。给药4、8周后处死实验兔,行软骨细胞凋亡率检测。结果模后第4周、8周,B、C、D三组软骨细胞凋亡率著低于A组(P<0.01),A、B、C、D组组间软骨细胞凋亡率比较,有显著性差异(P<0.01);B、C、D组组间比较,差异无统计学意义(P>0.05),但D组凋亡率明显小于B、C组。结论丹参加玻璃酸钠关节内注射,能明显降低兔膝关节软骨的细胞凋亡率,减缓膝关节软骨的退变,对软骨起保护效应。

用电化学方法研究红景天和丹参清除超氧阴离子自由基和羟基自由基的作用

利用单扫示波极谱法研究了红景天和丹参对活性氧自由基(O_2^-,OH)的清除作用.超氧阴离子自由基(O_2^-)通过邻苯三酚自氧化体系产生.羟基自由基由Fenton反应生成,用自旋捕集剂苯基叔丁基氮酮(PBN)捕捉生成的OH,以单扫示波极谱法检测自旋加合物.红景天和丹参样品对O_2^-和OH均有一定的清除作用,丹参对活性氧自由基的清除作用强于红景天.对O_2^-的清除能力为:红景天甙>吉林长白山红景天>西藏红景天;丹参对照品>安国丹参(Ⅰ)>安国丹参(Ⅱ).对OH的清除能力为:红景天甙>吉林长白山红景天>西藏红景天;安国丹参(Ⅱ)>安国丹参(Ⅰ)>丹参对照品.分别计算了各样品对自由基清除作用的IC_(50)值.

肢体缺血再灌注致肠损伤及丹参的保护作用

目的 :通过动物实验观察丹参对肢体缺血再灌注所致肠损伤的保护作用。方法 :80只Wistar大鼠随机分成正常对照组 ,缺血 3h和缺血 3h用丹参 3个组。实验组于缺血再灌注 1,2 ,3和 2 4h取材光镜对比观察和组织学评估。结果 :肢体缺血和再灌注后 ,实验组肠粘膜固有层毛细血管扩张、充血、大量多核白细胞浸润。大肠和小肠粘膜厚度和隐窝深度明显减少 (P <0 .0 1) ,小肠绒毛高度相对增加 (P <0 .0 1) ,隐窝细胞有变性坏死 ,用丹参组的病变明显轻于未用丹参组。结论

沙参麦冬汤对新生大鼠支气管肺发育不良的保护作用及其机制

目的:观察沙参麦冬汤作用下慢性支气管肺发育不良(BPD)新生大鼠肺的发育情况,阐明沙参麦冬汤对高体积分数氧(高氧)所致新生大鼠慢性BPD的影响及其作用机制。方法:200只新生大鼠分为对照组,模型组,地塞米松组,低和高剂量沙参麦冬汤组,每组40只。除对照组外,其余4组大鼠采用持续吸入高氧造模,沙参麦冬汤组和地塞米松组大鼠灌胃给予6.00和24.0 mg·kg-1沙参麦冬汤及0.75μg·kg-1地塞米松注射液,对照组大鼠给予等量生理盐水。给药21 d后检测各组大鼠肺组织湿质量/干质量(W/D)值,HE染色法观察各组大鼠肺组织病理形态表观,计算各组大鼠肺组织中辐射状肺泡计数(RAC),检测各组大鼠肺组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平,ELISA方法检测各组大鼠肺组织中细胞炎性因子白细胞间介素1(IL-1)、白细胞间介素10(IL-10)、肿瘤坏死因子α(TNF-α)水平,Western blotting法检测各组大鼠肺组织中促凋亡蛋白Bax、抗凋亡蛋白Bcl-2和caspase-3蛋白表达水平。结果:与对照组比较,模型组大鼠肺组织W/D值明显升高(t=3.144,P<0.05),可见明显肺水肿;氧暴露21 d后肺泡腔扩大、结构简化,间隔增厚,RAC明显减少(t=3.989,P<0.05),炎症细胞浸润,肺组织中MDA、SOD、GSH-Px、IL-1和TNF-α水平以及Bax和caspase-3蛋白表达水平明显升高(t=6.562,t=1.971,t=1.972,t=20.241,t=32.808,t=22.663,t=19.234,P<0.05),IL-10水平明显降低(t=7.857,P<0.05);与模型组比较,地塞米松组和不同剂量沙参麦冬汤组大鼠肺水肿程度改善,肺组织W/D值明显降低(t=1.018,t=2.691,t=0.760,P<0.05),肺泡结构紊乱情况减轻,肺组织中MDA、SOD、GSH-Px、IL-1和TNF-α水平以及Bax和caspase-3蛋白表达水平降低(t=6.562,t=1.971,t=1.972,t=20.241,t=32.808,t=22.663,t=19.234,P<0.05),IL-10水平和Bcl-2蛋白表达水平升高(t=7.857,t=7.743,P<0.05),且以高剂量沙参麦冬汤组效果为佳。结论:沙参麦冬汤能有效保护由高氧造成的新生大鼠支气管和肺的损伤。

丹参对暴发性肝衰竭大鼠肝损伤及肝再生的影响

目的:探讨丹参对暴发性肝衰竭(FHF)大鼠肝损伤及肝再生的影响。方法:Wistar大鼠随机分为4组:正常对照组、FHF组、丹参组、促肝细胞生长素(PHGF)组。FHF、丹参组和PHGF组动物模型采用TAA皮下注射,剂量按每kg体重600mg,2次,每次间隔24h,复制FHF动物模型。丹参组和PHGF组动物除皮下注射TAA外,于实验前3天至实验结束分别于皮下注射丹参注射液1ml/100g和PHGF1ml/100g,正常对照组和FHF组大鼠同时皮下注射生理盐水1ml/100g。FHF组、丹参组和PHGF组,于第2次注射TAA后24h,随机取大鼠各8只,腹主动脉取血测肝功能。迅速取肝组织,用10%甲醛液固定,制成石蜡切片,检测肝细胞有丝分裂指数(MI)和增殖细胞核抗原(PCNA)。结果:丹参具有降低FHF大鼠ALT、AST及TBil,显著提高肝细胞MI和PCNA的作用(P<0.05,P<0.01)。结论:丹参具有改善FHF大鼠肝功能和促进肝细胞增殖及再生的作用。