Efficient generation of pink-fruited tomatoes using CRISPR/Cas9 system

Tomato （Solanum lycopersicum） is the leading vegetable crop worldwide and an essential component of a healthy diet （Lin et al., 2014; Du et al., 2017）. Fruit color is regarded as one of the most important commercial traits in tomato （The Tomato Genome Consortium, 2012）. Consumers in different regions have different color preferences. For example, European and American consumers prefer red tomatoes, while pink tomatoes are more pop- ular in Asia countries, particularly in China and Japan （Ballester et al., 2010; Lin et al., 2014）. However, most of tomato breeding ma- terials are red-fruited, thus the generation of pink-fruited materials is very important for Asian tomato production. Metabolomics and genetics studies demonstrate that the pink trait results from the absence of yellow-colored flavonoid naringenin chalcone （NarCh） in the peels,

Efficient generation of the mouse model with a defined point mutation through haploid cell-mediated gene editing

Generation of mouse models carrying a defined point mutation,especially disease-related point mutations,is of considerable interest for research in biology and medicine.The standard method based on embryonic stem cell（ESC）-mediated homologous recombination（HR）is time-and labor-consuming.

High efficient generation of holographic stereograms based on wavefront recording plane

A computer generated holographic stereogram based on the wavefront recording plane （WRP） is presented. A WRP closed to the parallax image plane is introduced to record the complex amplitude in a small region for each point in the parallax image. By using three times of fast Fourier transform （FFT） to execute the Fresnel dif- fraction calculation between the WRP and the holographic stereogram plane, the object wave contributing to the hologram pattern can be achieved. The computation complexity of the proposed approach is dramatically reduced. The results show that the calculation time can be decreased by more than one order of magnitude.

Efficient generation of non-classical photon pairs in a hot atomic ensemble

We demonstrate the generation of non-classical photon pairs in a warm S-Rb atomic vapor （＇ell with no buffer gas or polarization preserving coatings via spontaneous four-wave mixing. We obtain the photon pairs with a 1/e correlation time of 40 ns and the violation of Cauchy-Sehwartz inequality by a factor of 23 - 3. This provides a convenient and efficient method to generate photon pair sources based on an atomic ensemble.

Low organic matter abundance and highly efficient hydrocarbon generation of saline source rock in the Qaidam Basin,NW China

The geochemical analysis and experimental simulation are comprehensively used to systematically study the hydrocarbon generation material,organic matter enrichment and hydrocarbon generation model of Paleogene source rock in the Western Qaidam Depression,Qaidam Basin,NW China.Three main factors result in low TOC values of saline lacustrine source rock of the Qaidam Basin:relatively poor nutrient supply inhibits the algal bloom,too fast deposition rate causes the dilution of organic matter,and high organic matter conversion efficiency causes the low residual organic carbon.For this type of hydrogen-rich organic matter,due to the reduction of organic carbon during hydrocarbon generation,TOC needs to be restored based on maturity before evaluating organic matter abundance.The hydrocarbon generation of saline lacustrine source rocks of the Qaidam Basin is from two parts:soluble organic matter and insoluble organic matter.The soluble organic matter is inherited from organisms and preserved in saline lacustrine basins.It generates hydrocarbons during low-maturity stage,and the formed hydrocarbons are rich in complex compounds such as NOS,and undergo secondary cracking to form light components in the later stage;the hydrocarbon generation model of insoluble organic matter conforms to the traditional“Tissot”model,with an oil generation peak corresponding to Ro of 1.0%.

Optimal design of linear switched reluctance motor for sea wave power generation

The switchless reluctance motor’s non-permanent magnet structure design ensures its high reliability in the marine environment;thus,it is a feasible solution for the generator of a sea wave power generation system.However,the corresponding thrust density and efficiency remain insufficient.This study focused on a new type of flat linear switched reluctance motor(LSRM),using the finite element software to establish a structural model,and optimized the design with the goal of improving the efficiency and energy density.The entropy method was adopted for sensitivity stratification to objectively select weights to avoid the influence of subjectively selected different proportional weights on the optimization results.Based on the entropy method,the sensitivity of different structural parameters was stratified,and the simulated annealing algorithm,response surface method,and single parameter scanning method were combined for optimization.Finally,the optimal structural size parameters of the motor were determined.Based on the two-dimensional finite element method,to simulate the electromagnetic performance of the reluctance motor under different operating conditions,such as thrust,loss,and efficiency,changes in motor performance before and after optimization were compared to verify the high power generation efficiency and energy density of the optimized linear motor.

The impact of dielectrics on the electrical capacity, concentration, efficiency ozone generation for the plasma reactor with mesh electrodes

This paper presents experimental results concerning the effect of dielectric type on ozone concentration and the efficiency of its generation in plasma reactor with two mesh electrodes.Three types of dielectric solid were used in the study; glass, micanite and Kapton insulating foil. The experiments were conducted for voltage ranges from 2.3 to 13 k V. A plasma reactor equipped with two 0.3×0.3 mm^2 mesh electrodes made of acid resistant AISI 304 mesh was used in the experiments. The influence of the dielectric type on the concentration and efficiency of ozone generation was described. The resulting maximum concentration of the ozone was about 2.70–9.30 g O3 m^-3, depending on the dielectrics used. The difference between the maximum and the minimum ozone concentration depends on the dielectric used,this accounts for 70% at the variance. The reactor capacity has also been described in the paper; total Ct and dielectric capacitance Cd depending on the dielectric used and its thickness.

Optimization of highly efficient terahertz generation in lithium niobate driven by Ti:sapphire laser pulses with 30 fs pulse duration

We systematically study the optimization of highly efficient terahertz（THz） generation in lithium niobate（LN）crystal pumped by 800 nm laser pulses with 30 fs pulse duration. At room temperature, we obtain a record optical-to-THz energy conversion efficiency of 0.43% by chirping the pump laser pulses. Our method provides a new technique for producing millijoule THz radiation in LN via optical rectification driven by joule-level Ti：sapphire laser systems, which deliver sub-50-fs pulse durations.

Efficient L-band ASE Generation Using a C/L-Band Coupler, an Optical Mirror and a FBG

A new structure for efficient and cost-effective L-band amplified spontaneous emission (ASE) generation was experimentally investigated and demonstrated using a C/L-band coupler, an optical mirror, and a fiber Bragg grating (FBG). The proposed structure has about 1.5 dB increase of the power around 1570 nm compared with conventional one. Various structures for power enhancement were also considered.

Effect of Different Raft Shapes on Hydrodynamic Characteristics of the Attenuator-Type Wave Energy Converter

A numerical simulation method based on CFD has been established to simulate the fully coupled motion for an atten-uator-type wave energy converter(WEC).Based on this method,a detailed parametric analysis has been conducted to investigate the design of the rafts.The effects of different parameters(wave parameters,structural parameters and PTO parameters)on the hydrodynamic characteristics of the attenuator-type WEC were studied in detail.The results show that in terms of wave parameters,there is an optimal wave period,which makes the relative pitching angle amplitude of the WEC reach the maximum,and the increase of wave height is conducive to the relative pitching angle amplitude of wave energy.Under different wave conditions,the relative pitch angle of the parallelogram raft device is the maximum.In terms of structural parameters,the parallelogram attenuator-type device has the optimal values in different relative directions,different distances and different apex angle,which makes the relative motion amplitude of the device reach the maximum,and the spacing and the apex angle have influence on the motion frequency of the device,while the relative direction has almost no influence on it.In terms of PTO parameters,there is an optimal damping coefficient,which makes the power generation efficiency of the WEC reach the maximum.The research results provide a valuable reference for future research and design of the attenuator-type WEC.

Amplification assisted difference frequency generation for efficient mid-infrared conversion based on monolithic tandem lithium niobate superlattice

We report the investigation on the performance of an amplification assisted difference frequency generation(AA-DFG) system driven by pulsed pump and continuous-wave primary signal lasers. A monolithic tandem lithium niobate superlattice was employed as the nonlinear crystal with a uniform grating section for the DFG process, followed by a chirp section for the optical parametric amplification process. The impacts of pump pulse shape, primary signal power, input beam diameter, and crystal structure on the pump-to-idler conversion efficiency of the AA-DFG system were comprehensively studied by numerically solving the coupled wave equations. It is concluded that square pump pulse and high primary signal power are beneficial to high pump-to-idler conversion efficiency. In addition, tighter input beam focus and smaller DFG length proportion could redeem the reduction in conversion efficiency resulting from wider acceptance bandwidths for the input lasers. We believe that such systems combining the merits of high stability inherited from cavity-free configuration and high efficiency attributed from the cascaded nonlinear conversion should be of great interest to a wide community,especially when the pulse shaping technique is incorporated.

Improving the intensity and efficiency of compressed echo in rare-earth-ion-doped crystal

We investigate the intensity and efficiency of a compressed echo, which is important in arbitrary waveform generation（AWG）. A new model of compressed echo is proposed based on the optical Bloch equations, which exposes much more detailed parameters than the conventional model, such as the time delay of the chirp lasers, the nature of the rare-earth-iondoped crystal, etc. According to the novel model of compressed echo, we find that reducing the time delay of the chirp lasers and scanning the lasers around the center frequency of the inhomogeneously broadened spectrum, while utilizing a crystal with larger coherence time and excitation lifetime can improve the compressed echo＇s intensity and efficiency. The theoretical analysis is validated by numerical simulations.

Enhanced wide-angle third-harmonic generation in flat-band-engineered quasi-BIC metagratings

Nonlinear metasurfaces and photonic crystals provide a significant way to generate and manipulate nonlinear signals owing to the resonance-and symmetry-based light-matter interactions supported by the artificial structures.However,the nonlinear conversion efficiency is generally limited by the angular dispersion of optical resonances especially in nonparaxial photonics.Here,we propose a metagrating realizing a quasi-bound-state in the continuum in a flat band to dramatically improve the third harmonic generation(THG)efficiency.A superior operating angular range is achieved based on the interlayer and intralayer couplings,which are introduced by breaking the mirror symmetry of the metagrating.We demonstrate the relation of angular dispersion between the nonlinear and linear responses at different incident angles.We also elucidate the mechanism of these offaxis flat-band-based nonlinear conversions through different mode decomposition.Our scheme provides a robust and analytical way for nonparaxial nonlinear generation and paves the way for further applications such as wide-angle nonlinear information transmission and enhanced nonlinear generation under tight focusing.

Study on Hydraulic Transmission of Offshore Low-speed Wind Energy Generator

For offshore hydraulic drive wind turbines,the problems of unsatisfactory speed control and low efficiency at low wind speeds are targeted.A low-speed high-torque radial piston pump is designed to replace the traditional fixed pump with a particular focus on its low-speed performance.The pump is characterized by small internal leakage at low wind speeds and high volumetric efficiency,which is beneficial to improve the power generation efficiency of the system.A new linear control method based on the PID algorithm and feedforward compensation was proposed to obtain the constant speed output control of variable motor at low wind speed.With the model for wind turbine and fixed pump-variable motor main drive system,the system was simulated and experimentally proved to verify the feasibility and anti-interference performance of the system control method at low wind speeds.A promising outcome was obtained on the response characteristics of system power and efficiency at low wind speeds.This can be a powerful technical support for the normal ustility of hydraulic drive wind turbines.

Chiral phonon activated spin Seebeck effect in chiral materials

Efficient generation of spin polarization is very important for spintronics and quantum computation. In chiral materials without magnetic order nor spin-orbit coupling, we find a new spin selectivity effect—chiral phonon activated spin Seebeck(CPASS)effect. Starting with the nonequilibrium distribution of chiral phonons under a temperature gradient, the CPASS coefficients are computed based on the Boltzmann transport theory. With both the phonon-drag and band transport contributions, the spin accumulations generated by the CPASS effect exhibit quadratic dependence on the temperature gradient. The strength of the CPASS effect and the relative magnitude of both contributions are tunable by the chemical potential modulation. The CPASS effect, which gives a promising explanation on the traditional chiral-induced spin selectivity effect, provides opportunities for the exploration of advanced spintronic devices based on chiral materials even in the absence of any magnetic order and spin-orbit coupling.

Innovated Inertia Control of DFIG with Dynamic Rotor Speed Recovery

High wind power penetration(WPP)is challenging system frequency stability.As a countermeasure,virtual inertia controls are introduced,utilizing kinetic energy(KE)stored in wind turbine generators(WTGs)for frequency regulation.Without restoration,generation efficiency of WTGs will be degraded after inertia contribution.To counter this issue,we propose an inertia control scheme of a doubly fed induction generator(DFIG),aiming at achieving dynamic inertia recovery regarding both KE and DC link energy.An asymmetrical droop control,referred to as the rate of change of frequency(RoCoF),is proposed for KE management.The upper boundary of droop gain is extended to give full play to converters and is revised,considering the system frequency state,to counter positive feedback issues induced by reversible gain regulation,which is restricted by KE to ensure stable operations as well.The inertial DC energy needed to cooperate with KE control regarding countering small fluctuations,is improved with an orderly recovery behavior.Case studies are conducted under dynamic wind conditions and the results indicate that with our proposed scheme,the ability of dynamic inertia recovery can be obtained,bringing DFIG higher generation efficiency and more adequate operation margin for sustained regulation.Essentially,the inertial frequency response and fluctuation suppression ability is well maintained.