In the search for the genome of egg drop syndrome virus (EDSV-76) Chinese strain AAV-2, part of restriction endonuclease physical map is analyzed, the complete genomic library is organized. On basis of this, the compl...In the search for the genome of egg drop syndrome virus (EDSV-76) Chinese strain AAV-2, part of restriction endonuclease physical map is analyzed, the complete genomic library is organized. On basis of this, the complete genome nucleotide sequences (32 838 bp in length, including terminal structures) are determined. The data analysis shows: compared with the other Adenovirases, strain AAV-2 has more disparity on genomic structure and the distribution of open reading frame (ORF). There are no clear El, E3 and E4 regions in AAV-2 genome. Two segments located at both ends of genome (l .1 kb and 8.3 kb in length respectively) have no homology with the other adenovirus genomes. In addition, strain AAV-2 genome lacks ORFs encoding El A, pV and pIX, which are common ORFs encoding early, lately proteins in Adenovirus. This reveals differences between EDSA-76, the sole standard strain of group Ⅲ Avian Adenovirases, and the other Avian Adenovirases for the first time. It will help the search for Avian Adenovirus and will also help the search of all Adenovirases.展开更多
用减蛋综合征-76(Egg drop syndrome’76)病毒WPDV205株感染31周龄莱航鸡,复制出了典型的减蛋综合征产蛋曲线,但蛋壳质量未见变化。从接种后第2周初,产蛋率由68.4％开始下降,至第4周降至43.5％。放射示踪实验表明,接种后7天,病毒分布于...用减蛋综合征-76(Egg drop syndrome’76)病毒WPDV205株感染31周龄莱航鸡,复制出了典型的减蛋综合征产蛋曲线,但蛋壳质量未见变化。从接种后第2周初,产蛋率由68.4％开始下降,至第4周降至43.5％。放射示踪实验表明,接种后7天,病毒分布于卵巢和输卵管的膨大部、峡部及阴道部,14天在肺和输卵管的膨大部,21天在阴道部,28天在肾和输卵管的漏斗部、峡部及阴道部。第4周后,除第8周在阴道部检出病毒外,在生殖道其它部位均未检出病毒。鸡群接种病毒后第1至4周从其蛋内容物中检测到病毒。展开更多
用NDV La Sota株、IBV M41株、EDS76 V HSH23株、AEV Van Roekel株为种毒,分别接种鸡胚或鸭胚,制备各种病毒抗原液,经福尔马林灭活后,采用FILTRON盒式超滤系统对制苗病毒抗原液进行浓缩,然后按一定比例混合,以司本80及吐温80为乳化剂,1...用NDV La Sota株、IBV M41株、EDS76 V HSH23株、AEV Van Roekel株为种毒,分别接种鸡胚或鸭胚,制备各种病毒抗原液,经福尔马林灭活后,采用FILTRON盒式超滤系统对制苗病毒抗原液进行浓缩,然后按一定比例混合,以司本80及吐温80为乳化剂,10号白油为佐剂,制成ND-IB-EDS76-AE四联灭活疫苗。对四联苗各项技术指标进行了测定,证明本疫苗安全、无任何副作用;免疫10-14 d后产生免疫力;ND部分效检,攻毒100％保护,每羽份含ND效价达50-123 PD50;IB部分效检,免疫21-28 d后攻毒保护率达80％-100％,IB HI效价≥1:64;EDS76效检,免疫后21 d HI效价≥1:128;AE效检,保护率达80％-100％。展开更多
文摘In the search for the genome of egg drop syndrome virus (EDSV-76) Chinese strain AAV-2, part of restriction endonuclease physical map is analyzed, the complete genomic library is organized. On basis of this, the complete genome nucleotide sequences (32 838 bp in length, including terminal structures) are determined. The data analysis shows: compared with the other Adenovirases, strain AAV-2 has more disparity on genomic structure and the distribution of open reading frame (ORF). There are no clear El, E3 and E4 regions in AAV-2 genome. Two segments located at both ends of genome (l .1 kb and 8.3 kb in length respectively) have no homology with the other adenovirus genomes. In addition, strain AAV-2 genome lacks ORFs encoding El A, pV and pIX, which are common ORFs encoding early, lately proteins in Adenovirus. This reveals differences between EDSA-76, the sole standard strain of group Ⅲ Avian Adenovirases, and the other Avian Adenovirases for the first time. It will help the search for Avian Adenovirus and will also help the search of all Adenovirases.
文摘用减蛋综合征-76(Egg drop syndrome’76)病毒WPDV205株感染31周龄莱航鸡,复制出了典型的减蛋综合征产蛋曲线,但蛋壳质量未见变化。从接种后第2周初,产蛋率由68.4％开始下降,至第4周降至43.5％。放射示踪实验表明,接种后7天,病毒分布于卵巢和输卵管的膨大部、峡部及阴道部,14天在肺和输卵管的膨大部,21天在阴道部,28天在肾和输卵管的漏斗部、峡部及阴道部。第4周后,除第8周在阴道部检出病毒外,在生殖道其它部位均未检出病毒。鸡群接种病毒后第1至4周从其蛋内容物中检测到病毒。
文摘用NDV La Sota株、IBV M41株、EDS76 V HSH23株、AEV Van Roekel株为种毒,分别接种鸡胚或鸭胚,制备各种病毒抗原液,经福尔马林灭活后,采用FILTRON盒式超滤系统对制苗病毒抗原液进行浓缩,然后按一定比例混合,以司本80及吐温80为乳化剂,10号白油为佐剂,制成ND-IB-EDS76-AE四联灭活疫苗。对四联苗各项技术指标进行了测定,证明本疫苗安全、无任何副作用;免疫10-14 d后产生免疫力;ND部分效检,攻毒100％保护,每羽份含ND效价达50-123 PD50;IB部分效检,免疫21-28 d后攻毒保护率达80％-100％,IB HI效价≥1:64;EDS76效检,免疫后21 d HI效价≥1:128;AE效检,保护率达80％-100％。
