The Use of HPLC in Determination of Endogenous Hormones in Anthers of Bitter Melon

A simple, rapid and perfect extraction and determination method for the endogenous hormones in anthers of bitter melon with reversed-phase high performance liquid chromatography （HPLC） has been developed. The HPLC system consisted of Hypersil ODS C 18 reverse phase column （150 mm × 4.6 mm, 5 μm） with a methanol gradient in 0.6% acetic acid and UV detector set at 254 nm. The results showed that the method was accurate and efficient.

Effect of Different Treatment on Anther Callus Formation and Browning in Balsam Pear （Mornordica charantia L.）

Brown callus derived from anther limited the application of anther culture in balsam pear. In order to establish a more perfect regeneration system from anther cultuer, the effects of low temperature pretreatment, 2,4-dichlorophenoxyacetic acid （2,4-D）, vitamin C （Vc） and silver nitrate （AgNO3） on callus induction and browning in anther culture of balsam pear （Momordiea charantia L.） were investigated. The results showed that after pretreatment at 4 ℃ for 1 day, callus induction rate was the highest and browning rate was the lowest. Anthers on MS medium supplemented with 2,4-D 0.5 mg/L formed more and better callus. The medium supplemented with Vc or AgNO3 was advantageous to the induction of callus and reduction of browning. When cultured on medium supplemented with 50 mg/L Vc or 5 mg/L AgNO3, callus induction rate was the highest and browning rate was rather low.

苦瓜耐热性相关农艺性状的筛选

从生长势、叶片热伤害程度、结果能力和存活率等方面对28份苦瓜材料进行耐热性综合鉴定,鉴定出不同苦瓜材料的耐热性强弱,再从中选取10个耐热性强、中、弱的苦瓜材料,分别调查死苗率、初花期、开花天数、第一雌花节位、茎粗以及果实性状中的果长、横径、肉厚、单瓜重等田间表型性状。结果表明:苦瓜品种(或自交系)的耐热性与初花期、开花天数、总产量、果肉横径、肉厚等呈显著正相关,与死苗率呈显著负相关,这些均可作为苦瓜成株期耐热性鉴定或筛选的农艺性状参考指标。