AIM:To investigate lipopolysaccharide(LPS) related signal transduction in interstitial cells of Cajal(ICCs) from mouse small intestine.METHODS:For this study,primary culture of ICCs was prepared from the small intesti...AIM:To investigate lipopolysaccharide(LPS) related signal transduction in interstitial cells of Cajal(ICCs) from mouse small intestine.METHODS:For this study,primary culture of ICCs was prepared from the small intestine of the mouse.LPS was treated to the cells prior to measurement of the membrane currents by using whole-cell patch clamp technique.Immunocytochemistry was used to examine the expression of the proteins in ICCs.RESULTS:LPS suppressed the pacemaker currents of ICCs and this could be blocked by AH6809,a prostaglandin E2-EP2 receptor antagonist or NG-Nitro-Larginine Methyl Ester,an inhibitor of nitric oxide(NO) synthase.Toll-like receptor 4,inducible NO synthase or cyclooxygenase-2 immunoreactivity by specific antibodies was detected on ICCs.Catalase(antioxidant agent) had no action on LPS-induced action in ICCs.LPS actions were blocked by nuclear factor kB(NF-kB) inhibitor,actinomycin D(a gene transcription inhibitor),PD 98059(a p42/44 mitogen-activated protein kinases inhibitor) or SB 203580 [a p38 mitogen-activated protein kinases(MAPK) inhibitor].SB 203580 also blocked the prostaglandin E2-induced action on pacemaker currents in ICCs but not NO.CONCLUSION:LPS inhibit the pacemaker currents in ICCs via prostaglandin E2-and NO-dependent mechanism through toll-like receptor 4 and suggest that MAPK and NF-kB are implicated in these actions.展开更多
[Objective]The paper was to investigate the mechanism of Bacillus coagulans preparation in improving production performance of laying hens in late period of laying.[Method]A total of 648 individuals of"Jingfen 1&...[Objective]The paper was to investigate the mechanism of Bacillus coagulans preparation in improving production performance of laying hens in late period of laying.[Method]A total of 648 individuals of"Jingfen 1"laying hens(66 weeks of age)in late period of laying were randomly divided into four groups,six replicates each group,and each group had 27 individuals of laying hens.The laying hens in control group were fed with basal diet,and those in experimental groupsⅠ,Ⅱ and Ⅲ were fed with the basal diets added with 3.33×10^6,1×10^7,3.33×10^7 CFU/g B.coagulans,respectively.The pretrial lasted one week,and the formal test lasted eight weeks.[Result]Compared with the control group,the laying rate in group Ⅰ increased significantly,and the feed-gain ratio in experimental group Ⅱ decreased noticeably while the spleen index increased remarkably;the alkaline phosphatase activities and blood calcium content in experimental groups Ⅰ,Ⅱ and Ⅲ increased significantly(P<0.05),while the triglyceride content decreased remarkably(P<0.05);the urea content in experimental groups Ⅰ and Ⅱ decreased obviously(P<0.05).Adding B.coagulans significantly increased the specific activity of amylase,lipase and protease in various intestinal mucosa of small intestine(P<0.05);adding B.coagulans significantly increased the content of follicle-stimulating hormone(FSH)and estradiol(E2),and significantly increased the mRNA expression of follicle stimulating hormone receptor gene(FSHR).[Conclusion]B.coagulans preparation could significantly improve the production performance of laying hens in late period of laying;appropriately enhance the immune capacity of laying hens;improve the serum biochemical indicators;increase the activity of digestive enzymes in the small intestine;and promote the release of gonadal hormone and the mRNA expression of FSHR gene in the ovary.展开更多
目的探讨影像组学在临床缓解的克罗恩病(Crohn's disease,CD)患者小肠黏膜愈合的诊断效能,并与磁共振活度指数(magnetic resonance index of activity,MaRIA)的诊断效能进行比较。方法收集2019年1月至2020年1月80例临床缓解的CD患...目的探讨影像组学在临床缓解的克罗恩病(Crohn's disease,CD)患者小肠黏膜愈合的诊断效能,并与磁共振活度指数(magnetic resonance index of activity,MaRIA)的诊断效能进行比较。方法收集2019年1月至2020年1月80例临床缓解的CD患者临床资料。临床缓解依据克罗恩活动度指数(Crohn's disease activity index,CDAI),CDAI<150分为缓解期。CD患者在入组时均行常规血液检测、结肠镜和小肠磁共振检查,并留取粪便测定FC浓度。内镜评分为克罗恩内镜活动度(Crohn's disease index of severity,CDEIS)。MaRIA根据MRI图像计算所得。通过K-近邻算法(K-Nearest Neighbor,KNN)建立KNN模型。分析两者特异度及敏感度,绘制受试者操作特征曲线(ROC),计算曲线下面积(AUC)。结果临床缓解的CD患者中MaRIA诊断小肠黏膜愈合的敏感度为0.802,特异度为0.775,AUC为0.804(P<0.05)。根据训练组建立影像组学KNN模型,其诊断小肠黏膜愈合的敏感度为0.865,特异度为0.6,AUC为0.801(P<0.05)。将KNN模型进行验证,验证组中KNN模型诊断敏感度0.727,特异度0.733,AUC为0.719(P<0.05)。结论影像组学模型和MaRIA均可较好诊断临床缓解的克罗恩病患者小肠黏膜愈合情况。展开更多
基金Supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education,Science and Technology,No.2012-0001335
文摘AIM:To investigate lipopolysaccharide(LPS) related signal transduction in interstitial cells of Cajal(ICCs) from mouse small intestine.METHODS:For this study,primary culture of ICCs was prepared from the small intestine of the mouse.LPS was treated to the cells prior to measurement of the membrane currents by using whole-cell patch clamp technique.Immunocytochemistry was used to examine the expression of the proteins in ICCs.RESULTS:LPS suppressed the pacemaker currents of ICCs and this could be blocked by AH6809,a prostaglandin E2-EP2 receptor antagonist or NG-Nitro-Larginine Methyl Ester,an inhibitor of nitric oxide(NO) synthase.Toll-like receptor 4,inducible NO synthase or cyclooxygenase-2 immunoreactivity by specific antibodies was detected on ICCs.Catalase(antioxidant agent) had no action on LPS-induced action in ICCs.LPS actions were blocked by nuclear factor kB(NF-kB) inhibitor,actinomycin D(a gene transcription inhibitor),PD 98059(a p42/44 mitogen-activated protein kinases inhibitor) or SB 203580 [a p38 mitogen-activated protein kinases(MAPK) inhibitor].SB 203580 also blocked the prostaglandin E2-induced action on pacemaker currents in ICCs but not NO.CONCLUSION:LPS inhibit the pacemaker currents in ICCs via prostaglandin E2-and NO-dependent mechanism through toll-like receptor 4 and suggest that MAPK and NF-kB are implicated in these actions.
文摘[Objective]The paper was to investigate the mechanism of Bacillus coagulans preparation in improving production performance of laying hens in late period of laying.[Method]A total of 648 individuals of"Jingfen 1"laying hens(66 weeks of age)in late period of laying were randomly divided into four groups,six replicates each group,and each group had 27 individuals of laying hens.The laying hens in control group were fed with basal diet,and those in experimental groupsⅠ,Ⅱ and Ⅲ were fed with the basal diets added with 3.33×10^6,1×10^7,3.33×10^7 CFU/g B.coagulans,respectively.The pretrial lasted one week,and the formal test lasted eight weeks.[Result]Compared with the control group,the laying rate in group Ⅰ increased significantly,and the feed-gain ratio in experimental group Ⅱ decreased noticeably while the spleen index increased remarkably;the alkaline phosphatase activities and blood calcium content in experimental groups Ⅰ,Ⅱ and Ⅲ increased significantly(P<0.05),while the triglyceride content decreased remarkably(P<0.05);the urea content in experimental groups Ⅰ and Ⅱ decreased obviously(P<0.05).Adding B.coagulans significantly increased the specific activity of amylase,lipase and protease in various intestinal mucosa of small intestine(P<0.05);adding B.coagulans significantly increased the content of follicle-stimulating hormone(FSH)and estradiol(E2),and significantly increased the mRNA expression of follicle stimulating hormone receptor gene(FSHR).[Conclusion]B.coagulans preparation could significantly improve the production performance of laying hens in late period of laying;appropriately enhance the immune capacity of laying hens;improve the serum biochemical indicators;increase the activity of digestive enzymes in the small intestine;and promote the release of gonadal hormone and the mRNA expression of FSHR gene in the ovary.
文摘目的探讨影像组学在临床缓解的克罗恩病(Crohn's disease,CD)患者小肠黏膜愈合的诊断效能,并与磁共振活度指数(magnetic resonance index of activity,MaRIA)的诊断效能进行比较。方法收集2019年1月至2020年1月80例临床缓解的CD患者临床资料。临床缓解依据克罗恩活动度指数(Crohn's disease activity index,CDAI),CDAI<150分为缓解期。CD患者在入组时均行常规血液检测、结肠镜和小肠磁共振检查,并留取粪便测定FC浓度。内镜评分为克罗恩内镜活动度(Crohn's disease index of severity,CDEIS)。MaRIA根据MRI图像计算所得。通过K-近邻算法(K-Nearest Neighbor,KNN)建立KNN模型。分析两者特异度及敏感度,绘制受试者操作特征曲线(ROC),计算曲线下面积(AUC)。结果临床缓解的CD患者中MaRIA诊断小肠黏膜愈合的敏感度为0.802,特异度为0.775,AUC为0.804(P<0.05)。根据训练组建立影像组学KNN模型,其诊断小肠黏膜愈合的敏感度为0.865,特异度为0.6,AUC为0.801(P<0.05)。将KNN模型进行验证,验证组中KNN模型诊断敏感度0.727,特异度0.733,AUC为0.719(P<0.05)。结论影像组学模型和MaRIA均可较好诊断临床缓解的克罗恩病患者小肠黏膜愈合情况。
文摘本试验旨在研究谷氨酰胺、壳聚糖及其二者复合添加对断奶仔猪小肠二糖酶活性、小肠绒毛高度及粪便菌群的影响。选取21日龄平均体重为(5.27±0.13)kg的断奶仔猪192头,随机分为4个处理,每个处理3个重复,每个重复16头猪。试验期14 d,分21～28 d和29～35 d 2个阶段,各组分别饲喂基础日粮(对照组)、基础日粮+1.00%谷氨酰胺(谷氨酰胺组)、基础日粮+0.04%壳聚糖(壳聚糖组)、基础日粮+1.00°%谷氨酰胺+0.04%壳聚糖(复合组)。结果表明:1)与对照组相比,复合组十二指肠乳糖酶、麦芽糖酶活性、空肠乳糖酶、蔗糖酶活性及回肠乳糖酶、蔗糖酶活性均显著升高(P<0.05);2)小肠绒毛高度在壳聚糖组、谷氨酰胺组、复合组依次呈升高趋势,但各组之间无显著性差异(P>0.05);3)复合组仔猪粪便中大肠杆菌、葡萄球菌数量降低,乳酸杆菌数量增加,但差异均不显著(P>0.05)。由此可知,同时添加1.00%谷氨酰胺和0.04%壳聚糖显著提高断奶仔猪小肠二糖酶的活性,增加绒毛高度,改善粪便菌群组成,且其效果均优于其他单独添加组。
