AIM: To investigate the effects of different parameters of gastric electrical stimulation (GES) on interstitial cells of Cajal (ICCs) and changes in the insulin-like growth factor 1 (IGF-1) signal pathway in streptozo...AIM: To investigate the effects of different parameters of gastric electrical stimulation (GES) on interstitial cells of Cajal (ICCs) and changes in the insulin-like growth factor 1 (IGF-1) signal pathway in streptozotocin-induced diabetic rats. METHODS: Male rats were randomized into control, diabetic (DM), diabetic with sham GES (DM + SGES), diabetic with GES1 (5.5 cpm, 100 ms, 4 mA) (DM + GES1), diabetic with GES2 (5.5 cpm, 300 ms, 4 mA) (DM + GES2) and diabetic with GES3 (5.5 cpm, 550 ms, 2 mA) (DM + GES3) groups. The expression levels of c-kit, M-SCF and IGF-1 receptors were evaluated in the gastric antrum using Western blot analysis. The distribution of ICCs was observed using immunolabeling for c-kit, while smooth muscle cells and IGF-1 receptors were identified using alpha-SMA and IGF-1R antibodies. Serum level of IGF-1 was tested using enzyme-linked immunosorbent assay. RESULTS: Gastric emptying was delayed in the DM group but improved in all GES groups, especially in the GES2 group. The expression levels of c-kit, M-SCF and IGF-1R were decreased in the DM group but increased in all GES groups. More ICCs (c-kit(+)) and smooth muscle cells (alpha-SMA(+)/IGF-1R(+)) were observed in all GES groups than in the DM group. The average level of IGF-1 in the DM group was markedly decreased, but it was up-regulated in all GES groups, especially in the GES2 group. CONCLUSION: The results suggest that long-pulse GES promotes the regeneration of ICCs. The IGF-1 signaling pathway might be involved in the mechanism underlying this process, which results in improved gastric emptying.展开更多
Background:This study aimed to demonstrate the effectiveness of transcutaneos tibial nerve stimulation(TTNS)in the treatment of bladder pain syndrome(BPS).Materials and methods:The data of 16 female patients,diagnosed...Background:This study aimed to demonstrate the effectiveness of transcutaneos tibial nerve stimulation(TTNS)in the treatment of bladder pain syndrome(BPS).Materials and methods:The data of 16 female patients,diagnosed with BPS in our clinic between 2019 and 2021 and had TTNS twice a week for 12 weeks,were retrospectively analyzed.Results:The mean age of the patients was 46.00±13.11 years,and the mean body mass index was 26.43±3.07 kg/m^(2).After the treatment,the median day time frequency of the patients decreased from 13.37(3.69)to 10.25(4.56)(p<0.001).Nocturia also decreased after treatment from 4.37(1.81)to 3.00(1.94)(p=0.001).The median voiding volume increased by 26.5 mL(p=0.001).The median of the patients'visual analog scale scores decreased after treatment(median of visual analog scale score changed from interquartile range 8[1]to 7[4])(p=0.001).In addition,the median interquartile range interstitial cystitis symptom index scores decreased from 17(4)to 15(10)(p=0.002).Conclusions:In this study it was demonstrated that TTNS is an alternative method that can be successfully applied before invasive methods in the treatment of BPS.展开更多
基金Supported by National Natural Science Foundation of ChinaNo.81270458 and No.81570488
文摘AIM: To investigate the effects of different parameters of gastric electrical stimulation (GES) on interstitial cells of Cajal (ICCs) and changes in the insulin-like growth factor 1 (IGF-1) signal pathway in streptozotocin-induced diabetic rats. METHODS: Male rats were randomized into control, diabetic (DM), diabetic with sham GES (DM + SGES), diabetic with GES1 (5.5 cpm, 100 ms, 4 mA) (DM + GES1), diabetic with GES2 (5.5 cpm, 300 ms, 4 mA) (DM + GES2) and diabetic with GES3 (5.5 cpm, 550 ms, 2 mA) (DM + GES3) groups. The expression levels of c-kit, M-SCF and IGF-1 receptors were evaluated in the gastric antrum using Western blot analysis. The distribution of ICCs was observed using immunolabeling for c-kit, while smooth muscle cells and IGF-1 receptors were identified using alpha-SMA and IGF-1R antibodies. Serum level of IGF-1 was tested using enzyme-linked immunosorbent assay. RESULTS: Gastric emptying was delayed in the DM group but improved in all GES groups, especially in the GES2 group. The expression levels of c-kit, M-SCF and IGF-1R were decreased in the DM group but increased in all GES groups. More ICCs (c-kit(+)) and smooth muscle cells (alpha-SMA(+)/IGF-1R(+)) were observed in all GES groups than in the DM group. The average level of IGF-1 in the DM group was markedly decreased, but it was up-regulated in all GES groups, especially in the GES2 group. CONCLUSION: The results suggest that long-pulse GES promotes the regeneration of ICCs. The IGF-1 signaling pathway might be involved in the mechanism underlying this process, which results in improved gastric emptying.
文摘Background:This study aimed to demonstrate the effectiveness of transcutaneos tibial nerve stimulation(TTNS)in the treatment of bladder pain syndrome(BPS).Materials and methods:The data of 16 female patients,diagnosed with BPS in our clinic between 2019 and 2021 and had TTNS twice a week for 12 weeks,were retrospectively analyzed.Results:The mean age of the patients was 46.00±13.11 years,and the mean body mass index was 26.43±3.07 kg/m^(2).After the treatment,the median day time frequency of the patients decreased from 13.37(3.69)to 10.25(4.56)(p<0.001).Nocturia also decreased after treatment from 4.37(1.81)to 3.00(1.94)(p=0.001).The median voiding volume increased by 26.5 mL(p=0.001).The median of the patients'visual analog scale scores decreased after treatment(median of visual analog scale score changed from interquartile range 8[1]to 7[4])(p=0.001).In addition,the median interquartile range interstitial cystitis symptom index scores decreased from 17(4)to 15(10)(p=0.002).Conclusions:In this study it was demonstrated that TTNS is an alternative method that can be successfully applied before invasive methods in the treatment of BPS.
