Gene transfer is one of the key techniques in gene therapy application. Unfortunately, it seems that by now, there still exists no approach with simplicity, easiness, efficiency and safety. A novel method for gene del...Gene transfer is one of the key techniques in gene therapy application. Unfortunately, it seems that by now, there still exists no approach with simplicity, easiness, efficiency and safety. A novel method for gene delivery, electro\|acupuncture needle\|mediated gene transfer which combined the Chinese traditional acupuncture with modern gene introduction, was developed. With acupuncture needle carrying exogenous gene into muscle after direct electronic stimuli, efficient gene delivery was achieved.展开更多
To investigate the feasibility of intramuscular electro-acupuncture-mediated VEGF gene transfer on experimental limb ischemia, the transcription and expression of VEGF gene have been detected with RT-PCR and immunohis...To investigate the feasibility of intramuscular electro-acupuncture-mediated VEGF gene transfer on experimental limb ischemia, the transcription and expression of VEGF gene have been detected with RT-PCR and immunohistochemistry staining, and the biological effect of VEGF gene inside the nat limb has been observed through vascular angiography. Results from angiography show that the transfer of VEGF gene into the rat with experimental limb ischemia can stimulate the formation of neovessel, establish collateral circulation, augment blood perfusion, and ameliorate distal limb necrosis. These findings reveal that intramuscular electro acupuncture-mediated VEGF gene transfer may present a new therapeutic modality for treating tissue ischemia.展开更多
文摘Gene transfer is one of the key techniques in gene therapy application. Unfortunately, it seems that by now, there still exists no approach with simplicity, easiness, efficiency and safety. A novel method for gene delivery, electro\|acupuncture needle\|mediated gene transfer which combined the Chinese traditional acupuncture with modern gene introduction, was developed. With acupuncture needle carrying exogenous gene into muscle after direct electronic stimuli, efficient gene delivery was achieved.
文摘To investigate the feasibility of intramuscular electro-acupuncture-mediated VEGF gene transfer on experimental limb ischemia, the transcription and expression of VEGF gene have been detected with RT-PCR and immunohistochemistry staining, and the biological effect of VEGF gene inside the nat limb has been observed through vascular angiography. Results from angiography show that the transfer of VEGF gene into the rat with experimental limb ischemia can stimulate the formation of neovessel, establish collateral circulation, augment blood perfusion, and ameliorate distal limb necrosis. These findings reveal that intramuscular electro acupuncture-mediated VEGF gene transfer may present a new therapeutic modality for treating tissue ischemia.
