Studies of Ce(Ⅲ)-ALC-F Interacting with Herring Sperm DNA by Electrochemical, Fluorimetric and UV-spectrophotometric Method

Ce(Ⅲ)-ALC-F complex can react with hsDNA to form an electrochemically non-active supermolecular complex Ce(Ⅲ)-ALC-F-DNA in the buffer solution of (CH2)6N4(pH=4.9), which results in the decrease of the peak current of Ce(Ⅲ)-ALC-F. This method can be applied to determine DNA concentration. In addition, by using fluorimetric and UV-spectrophotometric methods with studies of denatured DNA and the effect of NaCl solution , it is also found that the binding mode is intercalation.

钕(Ⅲ)-桑色素配合物与DNA相互作用的电化学与荧光光谱研究

采用电化学和荧光光谱法对Nd(Ⅲ)-桑色素配合物与DNA的相互作用进行了研究。实验发现在pH为6.0的Tris-HCl缓冲溶液中,加入溴化十六烷基三甲铵(CTMAB)微乳液,Nd(Ⅲ)能与桑色素形成配合物并在-1.55 V(vsSCE)处产生1个灵敏的极谱波。Nd(Ⅲ)-桑色素配合物与DNA以嵌入方式生成一种非电活性超分子复合物Nd(Ⅲ)-桑色素-DNA,导致Nd(Ⅲ)-桑色素的峰电流降低,峰电流在一定范围内与DNA质量浓度呈线性关系,线性范围为1～15 mg/L,检出限为0.08 mg/L。在pH为6.0的Tris-HCl缓冲溶液中,体系的荧光强度最强,最大激发波长为392 nm,最大发射波长为535 nm。配合物荧光强度在DNA存在时增强,并与DNA的质量浓度在1～10 mg/L范围内呈线性关系,检出限为0.10mg/L,可用于DNA的测定。