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Detection of the End Point Temperature of Thermal Denatured Protein in Fish and Chicken Meat Through SDS-PAGE Electrophoresis 被引量:4
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作者 GAO Hongwei MAO Mao +2 位作者 LIANG Chengzhu LIN Chao XIANG Jianhai 《Journal of Ocean University of China》 SCIE CAS 2009年第1期95-99,共5页
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in stu... Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated heating thermal denatured proteins under the same temperature and processing time were studied. The electrophoresis patterns of thermal denatured proteins determined through repeated heating at the same temperature did not exhibit any change. For the detection of cooked fish and meat samples, they were subjected to applying the SDS-PAGE method, which revealed an EPT ranging from 60 ℃ to 80 ℃ . 展开更多
关键词 end point temperature DETECTION sds-page electrophoresis
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Optimization of the Electrophoresis Tricine-SDS-PAGE for Simultaneous Detection of Protein and Peptide in Artisinal Cheese
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《Journal of Agricultural Science and Technology(B)》 2019年第6期423-427,共5页
The objective of this paper was to optimize an electrophoretic methodology (Tricine-SDS-PAGE) to monitor the degree of caseins hydrolysis during the maturation time of artisanal cheese. A cheaper and easier method was... The objective of this paper was to optimize an electrophoretic methodology (Tricine-SDS-PAGE) to monitor the degree of caseins hydrolysis during the maturation time of artisanal cheese. A cheaper and easier method was obtained using small samples and micro-quantities of reagents. Simultaneous detection of proteins and peptides (100 kDa to 1 kDa) in the same gel was another advantage of the method. Initially, protein extraction was performed with 2 mg of lyophilized cheese dissolved in 1.0 mL of sample electrophoretic buffer for 1 h under stirring. After that, the Eppendorf tubes of the samples were kept at -4 °C for 4 h with additional centrifugation at 5,433 ×g for 2 min. This defatting process using centrifugation/refrigeration promoted a good separation of proteins and peptides from the fat layer. After this step, 30 L of the supernatant of the protein extracts was applied to the electrophoresis gel. The results revealed a clear image of protein and peptides in the polyacrylamide gels and allowed an excellent response of the distribution of casein bands (α, β and κ) and the exposure peptide in cheese. The utilization of artisanal cheese as a pilot study of molecular protein analysis could be helpful for further correlation of the fingerprint of protein-peptide profile with taste quality. 展开更多
关键词 Artisanal cheese electrophoresis maturation time PROTEOLYSIS
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Study of Peganum harmala Genetic Diversity Based on Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) Analysis
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作者 H. Rostami-Ahmadvandi D. Kahrizi +3 位作者 A. R. Zebarjadi A. Mostafaie F. Sohrabi-Babahadi S. Kiani 《Journal of Agricultural Science and Technology(A)》 2011年第8期1300-1302,共3页
Syrian Rue (Peganum harmala L.) is grown in semi-arid conditions. Its origin is Central Asia and has several medicinal uses especially in seeds. Study of genetic diversity for this plant is important for researches ... Syrian Rue (Peganum harmala L.) is grown in semi-arid conditions. Its origin is Central Asia and has several medicinal uses especially in seeds. Study of genetic diversity for this plant is important for researches in future. The electrophoresis of seed storage protein is a method to investigate SDS-PAGE and to classify plant varieties because these proteins are highly preserved. This study was conducted to determine the seed storage profiles of 20 Peganum harmala accessions that have been collected in some regions in lran. Extracted proteins were analyzed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) as a biochemical marker. For this purpose, the samples were first crushed and seed protein was extracted by extraction buffer then total soluble proteins were resolved on 15% SDS polyacrylamide gels. The gels showed 20 bands that were high polymorphism among the accessions. The noticeable differences for example were observed in area with 38 KDa. The result of analysis showed that the accessions were classified in three groups (13, 3 and 4 accessions in the first, second and third groups respectively). 展开更多
关键词 Peganum harmala sds-page genetic diversity cluster analysis
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Prevalence and Study of the Clonality of Extended-Spectrum Beta-Lactamase-Producing Klebsiella pneumoniae Strains in Neonatology at the University Hospitals of Abidjan by the Pulsed Field Gel Electrophoresis and the Quantitative Antibiogram
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作者 Valérie M’Bengue Gbonon Sidjè Arlette Afran +6 位作者 Stanislas Assohoun Egomli Djeda Franck Arnaud Gnahoré Aboubakar Sylla N’Golo David Coulibaly Nathalie Guessennd Assanvo Simon-Pierre N’Guetta Mireille Dosso 《Advances in Microbiology》 CAS 2024年第9期416-429,共14页
Background: ESBL-producing strains of Klebsiella pneumoniae, one of the main causes of nosocomial and hospital-acquired infections, are commonly associated with therapeutic impasses. Surveillance of these multidrug-re... Background: ESBL-producing strains of Klebsiella pneumoniae, one of the main causes of nosocomial and hospital-acquired infections, are commonly associated with therapeutic impasses. Surveillance of these multidrug-resistant pathogens is a crucial tool for controlling and preventing infections. This surveillance involves the use of appropriate molecular and phenotypic typing techniques. The choice of techniques is based on criteria such as discriminatory power, intra- and inter-laboratory reproducibility, epidemiological concordance, ease of use and cost. The aim of our study was to identify clusters of Extended-Spectrum Beta-Lactamase-producing Klebsiella pneumoniae (ESBL-K. pneumoniae) strains circulating in neonatology using quantitative antibiogram (QA) and Pulsed Field Gel Electrophoresis (PFGE). Materials and Methods: This cross-sectional study included 55 K. pneumoniae strains isolated from a total of 513 samples. These various samples are taken from newborns, healthcare personnel, and the environment. K. pneumoniae identification followed standard bacteriological procedures and was confirmed using the Vitek® 2 (bioMérieux). The detection of the ESBL phenotype was performed using the synergy test. QA and PFGE were used to identify clonal relationships between the various strains isolated. Concordance between these two methods was assessed by calculating Cohen’s KAPPA coefficient and Simpson’s diversity index. Results: Among the 55 K. pneumoniae strains included in this study, 58.2% (32/55) were found to be Extended-Spectrum Beta-Lactamase (ESBL) producers. Most of these strains were isolated from neonatal samples (blood samples and rectal swabs). The quantitative antibiogram method applied to 28 out of the 32 ESBL-producing strains revealed that the isolates were grouped into 5 clusters. Pulsed Field Gel Electrophoresis performed on a total of 16 ESBL-producing strains showed the existence of four profiles. A perfect concordance was observed between the two methods. Conclusion: The results of this study highlighted the existence of clonal strains of various origins within neonatology units. 展开更多
关键词 Resistance-Clone-Klebsiella pneumoniae-Pulsed Field Gel electrophoresis-Quantitative Antibiogram
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Analysis of Resistance-related Proteins in Rice Against Brown Planthopper by Two-dimensional Electrophoresis 被引量:12
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作者 陈荣智 翁清妹 +2 位作者 黄臻 祝莉莉 何光存 《Acta Botanica Sinica》 CSCD 2002年第4期427-432,共6页
A recombinant inbred population (RI) was constructed from a cross between B5, an introgression. line from the wild rice Oryza officinalis Wall. ex Watt, and susceptible cultivar Minghui 63 ( O. sativa L.). The brown p... A recombinant inbred population (RI) was constructed from a cross between B5, an introgression. line from the wild rice Oryza officinalis Wall. ex Watt, and susceptible cultivar Minghui 63 ( O. sativa L.). The brown planthopper ( BPH) resistances of RI lines were evaluated. Based on bulked segregant analysis (BSA), two protein bulks were made by extracting proteins from equally mixed seedlings of extremely resistant and susceptible plants selected from the RI population, respectively. Two-dimensional electrophoresis was used to detect the changes of polypeptide pattern. Results showed that a protein P40 ( pI 6.3, Mw 40 kD) was significantly reduced or vanished after BPH infestation for 48 h in the susceptible bulk, while it remained uninfluenced in the resistant bulk. In connection with the physiological changes of the resistant and susceptible lines subjected to BPH sucking, we suppose that the protein P40 is related to the interaction responses of lice plants to BPH infestation. 展开更多
关键词 RICE recombinant inbred lines brown planthopper resistance two-dimensional electrophoresis
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Research on Sex and Tissue Differences in Isozymic Phenotype of Varicorhinus macrolepis through Isozyme Electrophoresis 被引量:6
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作者 庞秋香 庞书香 +5 位作者 赵博生 宿烽 孙欢欢 颜越 吕志林 刘龙 《Agricultural Science & Technology》 CAS 2010年第5期101-105,共5页
Polyacrylamide gel electrophoresis (PAGE) and biochemical staining method were used in this study for the analysis on malate dehydrogenase (MDH,E.C. 1.1.1.37) isozymes zymogram in 11 different types of tissues of male... Polyacrylamide gel electrophoresis (PAGE) and biochemical staining method were used in this study for the analysis on malate dehydrogenase (MDH,E.C. 1.1.1.37) isozymes zymogram in 11 different types of tissues of male and female Varicorhinus macrolepis. It had been found for the first time that the phenotype of malate dehydrogenase (MDH),acid phosphatase (ACP) and superoxide dismutase (SOD) showed difference between male and female V. macrolepis,and there was no difference among different individuals in the same sex. Therefore,the electrophoresis band of malate dehydrogenase,acid phosphatase and superoxide dismutase could be used as an indicator for the identification of gender and tissues of V. macrolepis,which would provide basic data for the developmental genetics,variety improvement and directed breeding of V. macrolepis groups,thus facilitating the development and protection of this valuable fish species. 展开更多
关键词 Varicorhinus macrolepis Malate dehydrogenase Acid phosphatase Superoxide dismutase Polyacrylamide gel electrophoresis (PAGE)
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Study on the Optimization of Two-dimensional Electrophoresis Technology System for Rapeseed Proteome 被引量:4
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作者 王丽娟 任学敏 +4 位作者 杜艳慧 张其彬 罗水忠 姜绍通 郑志 《Agricultural Science & Technology》 CAS 2011年第5期625-629,共5页
[Objective] The research aimed to establish the two-dimensional electrophoresis(2-DE)technology which was suitable for the rapeseed proteome research.[Method] Xiangyou 17 was as the material.The sample preparation m... [Objective] The research aimed to establish the two-dimensional electrophoresis(2-DE)technology which was suitable for the rapeseed proteome research.[Method] Xiangyou 17 was as the material.The sample preparation method,gel concentration and loading amount,etc.in 2-DE technology were optimized.[Result] The best extraction method of total protein of rapeseed was TCA-acetone method,and the protein spots on 2-DE map were the most.When IPG strip(pH 3-10)and 12% gel were used,and the loading amount was 250 μg,the two-dimensional electrophoresis map with the clear background,good repeatability and high protein spot resolution was obtained.[Conclusion] The research laid the foundation for carrying out the rapeseed proteomics research. 展开更多
关键词 RAPESEED PROTEOME Two-dimensional electrophoresis System optimization
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Single Cell Gel Electrophoresis Assay of Porcine Leydig Cell DNA Damage Induced by Zearalenone 被引量:1
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作者 甄建伟 刘青 +5 位作者 顾建红 袁燕 刘学忠 王捍东 刘宗平 卞建春 《Agricultural Science & Technology》 CAS 2012年第7期1587-1590,1594,共5页
[Objective] This study aimed to investigate the effect of zearalenone (ZEN) on DNA damage of porcine leydig cells. [Method] Porcine leydig cells cultured in vitro were collected to determine the median lethal dose (LD... [Objective] This study aimed to investigate the effect of zearalenone (ZEN) on DNA damage of porcine leydig cells. [Method] Porcine leydig cells cultured in vitro were collected to determine the median lethal dose (LD50) of ZEN with tetrazolium-based colorimetric assay (MTT assay). Comet assay was carried out to detect the DNA damage of porcine leydig cells exposed to at 0 (negative group), 1, 5, 10, 20, 40 μmol/L of ZEN. [Result] The percentage of cell tail was 16.67%, 34.00%, 40.67%, 52.00% and 64.67% under 0, 1, 5, 10 and 20 μmol/L of ZEN, respectively; the differences between the percentages of cell tail in various experimental groups had extremely significant statistical significance compared with the negative group (P<0.01), showing a significant dose-effect relationship; Tail length in various groups was 57.60±4.78, 57.75±6.25, 78.97±5.83, 100.50±6.94 and 146.83±12.31 μm, respectively; Tail DNA % in various groups was 21.29±2.25%, 22.24±2.43%, 31.21±6.27%, 37.45±4.33% and 60.68±9.83%, respectively; Tail length and Tail DNA % in experimental groups with ZEN concentration above 5 μmol/L showed significant differences (P<0.05) compared with the negative group, which showed an upward trend with the increase of ZEN concentration. [Conclusion] ZEN has genotoxic effect on porcine leydig cells, which can cause DNA damage, with a significant dose-effect relationship. 展开更多
关键词 Leydig cells ZEARALENONE DNA damage Comet assay (Single cell gel electrophoresis assay)
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Interactions Between Thrombin and Natural Proudcts of Coreopsis tinctoria Nuttt. and Cistanche deserticola Ma. in Capillary Zone Electrophoresis 被引量:1
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作者 刘一 张媛 +3 位作者 刘晓明 凌笑梅 屠鹏飞 崔景荣 《Journal of Chinese Pharmaceutical Sciences》 CAS 2006年第1期38-44,共7页
Aim A capillary zone electrophoretic method (CZE) was used to determine the interactions between natural products and thrombin. Methods Samples containing natural products and thrombin at various ratios were incubat... Aim A capillary zone electrophoretic method (CZE) was used to determine the interactions between natural products and thrombin. Methods Samples containing natural products and thrombin at various ratios were incubated at 25 ℃ and then were separated by CZE with Tris-acetate buffer at pH 7.2. Each run could be accomplished within 5 min. Results In CZE, the peak width broadened due to the affinity interaction between natural products and thrombin. Compared with positive and negative control, the natural products (CB-1, CB-2) from Coreopsis tinctoria Nuttt. interacted with thrombin; CB-3 from Coreopsis tinctoria Nuttt. and HC-1, HC-2, HC-3 from Cistanche deserticola Ma. did not bind to thrombin. Both qualification and quantification characterizations of the binding were determined. Conclusion The established method is capable of sensitive and fast determination of natural products and thrombin interactions, it can be employed as an alternative method. 展开更多
关键词 capillary zone electrophoresis Coreopsis tinctoria Nuttt. Cistanche deserticola Ma INTERACTIONS THROMBIN
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Optimization of SSR-PCR Non-denatured Polyacrylamide Gel Electrophoresis Conditions in Kernelled Apricot 被引量:1
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作者 艾鹏飞 方闪闪 +1 位作者 吴学敏 靳占忠 《Agricultural Science & Technology》 CAS 2010年第9期50-52,139,共4页
[Objective] The aim was to optimize the SSR-PCR non-denatured polyacrylamide gel electrophoresis conditions in kernelled apricot.[Method]25 accessions of kernelled apricot and three accessions of edible apricot were s... [Objective] The aim was to optimize the SSR-PCR non-denatured polyacrylamide gel electrophoresis conditions in kernelled apricot.[Method]25 accessions of kernelled apricot and three accessions of edible apricot were selected as experimental materials to screen the repeatable SSR loci with high polymorphism by the use of SSR markers combined with non-denatured polyacrylamide gel electrophoresis.And the effect of different factors on electrophoresis conditions was compared to explore the optimal SSR-PCR non-denatured polyacrylamide gel electrophoresis conditions in kernelled apricot.[Result]The optimal non-denatured polyacrylamide gel electrophoresis conditions for SSR-PCR were established as follows:polyacrylamide gel concentration 6%,the ratio of acrylamide to bisacrylamide 29∶1,electrophoresis at 1 000 V for 2-3 h,and staining for 15 min within 0.1% AgNO3.[Conclusion]The optimum electrophoresis system has provided some technical foundations to further study the phylogenetic relationship of kernelled apricots by SSR markers. 展开更多
关键词 Kernelled Apricot SSR markers Polyacrylamide gel electrophoresis Silver staining
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The Superiority of Like-rocket Immunoelectrophoresis Using in Single Cell Gel Electrophoresis Assay 被引量:1
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作者 穆淑梅 康现江 郭明申 《Agricultural Science & Technology》 CAS 2010年第7期17-19,共3页
[Objective] The like-rocket immunoelectrophoresis was used to explore a new feasible electrophoresis method for single cell gel electrophoresis assay (comet assay).[Method] The like-rocket immunoelectrophoresis was ... [Objective] The like-rocket immunoelectrophoresis was used to explore a new feasible electrophoresis method for single cell gel electrophoresis assay (comet assay).[Method] The like-rocket immunoelectrophoresis was used for single cell gel electrophoresis assay to detect DNA damage at single cell level,then it was compared with traditional electrophoresis method to analyze its advantage and disadvantages.[Result] Under cell DNA undamaged state,the results of two electrophoresis methods were consistent.When cell DNA was damaged,the comet tail divergence of some cells under traditional electrophoresis method were drifted,however,the comet tail image of like-rocket immunoelectrophoresis was concentrated and not shifted.[Conclusion] The like-rocket immunoelectrophoresis had some advantages. 展开更多
关键词 Single cell gel electrophoresis DNA damage Like-rocket immunoelectrophoresis
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Electrophoresis Analysis on the Protein Expression in Heteromorphic Leaves of Populus euphratica Oliv. 被引量:1
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作者 岳宁 郑彩霞 Cai-xia 《Agricultural Science & Technology》 CAS 2010年第5期41-45,共5页
[Objective] This study was to elucidate the cellular and molecular mechanism of the development of heteromorphic leaves of Populus euphratica Oliv. [Method] By employing SDS-PAGE and 2-demensional electrophoresis (2-D... [Objective] This study was to elucidate the cellular and molecular mechanism of the development of heteromorphic leaves of Populus euphratica Oliv. [Method] By employing SDS-PAGE and 2-demensional electrophoresis (2-DE) techniques,proteins in various heteromorphic leaves from the same adult tree of P. euphratica were isolated and separated to the electrophoresis technique suitable for the separation and analysis of proteins in leaves of P. euphratica tree. [Results] There were significant differences in the expressions of proteins in various heteromorphic leaves of P. euphratica tree. SDS-PAGE pattern showed that bands of proteins with molecular weight of 57.2,13.2,30.2,23.9 and 33.3 kDa were remarkably different. 2-D electrophoresis pattern presented that proteins in leaves of P. euphratica tree mainly belong to acidic proteins distributed at pH value of 5.0-6.5 and with molecular weight of 20-40 kDa; totally 73 different protein spots were observed,of which 51 were up expressed and other 22 were down expressed in the serrated ovate leaves. [Conclusion] Based on these results,we speculate that regulated gene expression in leaves of P. euphratica tree results in the generation of different shapes of leaves,in order to adapt to the surroundings better. 展开更多
关键词 Populus euphratica Oilv. Heteromorphic leaves sds-page 2-demensional electrophoresis
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Enantioseparation of Several PharmaceuticalRacemates with High PerformanceCapillary electrophoresis
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作者 李方 靳慧 +2 位作者 戴荣继 顾峻岭 傅若农 《Journal of Beijing Institute of Technology》 EI CAS 1997年第3期239-243,共5页
With high performance capillary electrophoresis using -cyclodextrin or its deriveatives as the chiral selectors, five pairs of drug enantiomers were separated, The PH of the back- gmund electrolyte and the chiral sele... With high performance capillary electrophoresis using -cyclodextrin or its deriveatives as the chiral selectors, five pairs of drug enantiomers were separated, The PH of the back- gmund electrolyte and the chiral selector concentrations were optimized; and the effect of organic modifier on separation of chlorpheniramine enantiomers was also inver 展开更多
关键词 capillary electrophoresis ENANTIOSEPARATION chiral drugs
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Electrophoresis of poly(dT)_(20) through α-hemolysin nanopore in high concentration potassium chloride solution
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作者 司伟 杨浩杰 +4 位作者 纪安平 李堃 沙菁 刘磊 陈云飞 《Journal of Southeast University(English Edition)》 EI CAS 2016年第4期496-501,共6页
Experiments of poly(dT)20 electrophoresis throughα-hemolysin nanopores were performed to unveil the electrophoretic transport mechanism of DNA through nanopores in high concentration potassium chloride solution. It... Experiments of poly(dT)20 electrophoresis throughα-hemolysin nanopores were performed to unveil the electrophoretic transport mechanism of DNA through nanopores in high concentration potassium chloride solution. It is found that there are two obvious current blockades induced by poly(dT)20 translocation and collision events. Both blockade currents increase linearly with the applied bias voltage. However, the normalized blockade currents are almost kept the same although variable bias voltages are applied. The collision time of poly(dT)20 in the luminal site of the pore remains constant for different voltages. The translocation speed of poly(dT)20through the nanopore decreases with the increase of bias voltage. It is because as the potential increases, the drag force on the homopolymer helps it to crumple into a cluster much easier due to the poor stacking of thymine residues compared with homopolymers consisting of other nucleotides. Molecular dynamics simulations further confirm the experimental results. Increasing the applied bias voltage can slowdown the translocation velocity of the flexible poly(dT)20, which favors increasing the precision of single molecule detection by using nanopores. 展开更多
关键词 NANOPORE transport speed ionic current electrophoresis
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A Peptides Migration Model in capillary Zone Electrophoresis
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作者 梁恒 王正刚 +2 位作者 顾峻岭 傅若农 林炳承 《Journal of Beijing Institute of Technology》 EI CAS 1996年第1期35-39,共5页
A peptides migration model based on the principle of mechanics is presented in capillary zone electrophoresis (CZE). It is shown that the migration that the (tr) is a function of electric (Q), relative molecular mass ... A peptides migration model based on the principle of mechanics is presented in capillary zone electrophoresis (CZE). It is shown that the migration that the (tr) is a function of electric (Q), relative molecular mass (Mr), conformation parameter (Rc) of peptides and electrophoretic condition parameter(A). The conformation parameter is introduced to characterize multifarious shapes owing to the complex conlormation and the various kinds of macromolecules, where Rc≥1/3. The parameters A and Rc can be obtained from experimental data. The times of migration of the nine standard peptides were measured in pH 2.5buffer on different electrophoretic conditions in CZE. The experimental results agreed well with the theoretical prediction. 展开更多
关键词 capillary zone electrophoresis migration model conformation parameter
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Separation of Nucleotides in Swine Tissues Using Capillary Zone Electrophoresis
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作者 顾峻岭 刘玉 +2 位作者 傅若农 鲁红军 张燕婉 《Journal of Beijing Institute of Technology》 EI CAS 1994年第2期155-161,共7页
Capillary zone electrophoresis has been applied to the analysis of nucleotides. The effects of buffer concentration. pH and other operating conditions on the separation were investigated and optimized. By using the me... Capillary zone electrophoresis has been applied to the analysis of nucleotides. The effects of buffer concentration. pH and other operating conditions on the separation were investigated and optimized. By using the method, separation and identification of nuclotides in swine tissues were completed. 展开更多
关键词 SEPARATION nucleotides/capillary zone electrophoresis
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AN ALLOZYME ELECTROPHORESIS STUDY ON ELEVEN SPECIES OF MEGOPHRYINAE IN CHINA 被引量:2
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作者 刘万兆 杨大同 《Zoological Research》 CAS CSCD 1994年第S1期182-192,0,共12页
Allozymes of eleven species of Megophryinae in China were examined electrophoretically to investigate genetic diversity and phylogenetic relationships. Fourteen enzymes, presumptively coded by 24 loci were detected to... Allozymes of eleven species of Megophryinae in China were examined electrophoretically to investigate genetic diversity and phylogenetic relationships. Fourteen enzymes, presumptively coded by 24 loci were detected to be variable. Gene frequencies of each population at each locus were presented. The commonly used measure of genetic diversity, the average heterozygosity (H) were calculated based on gene frequencies. The results indicated that Megophryinae had a high level of genetic diversity in amphibians, an average H of 0.18, ranging from 0.058 to 0.28. Nei's (1978) genetic distances(Nei's D) were calculated for all possible population pairs. A dendrogram of 13 populations representing 11 species, 3 genera of Megophryinae were derived and presented by using UPGMA, based on Nei' s D. The assignment of Ophryophryne as a distinct genus were supported by an average Nei's D of 1.4067 which separated O. microstoma from all other populations.Subdivision of Brachytarsophrys from Megophrys was not supported by this study. Within Megophrys, three groups were recognized: (1)M. lateralis, M. giganticus and M. longipes; (2)M. palpebralespineosa, M. boettgeri and M. parva;(3) M. minor and M. kuatunensis. Three populations of M. omeimontis were closely related and share a clade independent from all other Megophrys, and B. feae as well. 展开更多
关键词 AMPHIBIA PELOBATIDAE Megophryinae Genetic diversity Phylogenetic relationships Allozyme electrophoresis China
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Simultaneous Determination of Clonidine Hydrochloride,Hydrochlorothiazide, and Rutin in Zhenju Jiangya Tablet by Capillary Electrophoresis 被引量:4
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作者 苏圣民 郁韵秋 《Journal of Chinese Pharmaceutical Sciences》 CAS 2005年第3期173-175,共3页
Aim To develop a method for the determination of three drug components: clonidine hydrochloride, hydrochlorothiazide and rutin in Zhenju Jiangya tablet. Methods An uncoated capillary tube was used to analyze under 20... Aim To develop a method for the determination of three drug components: clonidine hydrochloride, hydrochlorothiazide and rutin in Zhenju Jiangya tablet. Methods An uncoated capillary tube was used to analyze under 20 kV voltage at 20 ℃. The samples were introduced into the capillary tube by hydrodynamic mode applying 50 kPa for 5 s and detected at 210 nm. Results The linear ranges of clonidine hydrochlofide, hydrochlorothiazide, and rutin were 10 μg· mL^-1 - 100μg· mL^-1, 30μg· mL^-1 - 300 μg· mL^- 1, and 30μg · mL^-1 - 300μg · mL^-1, respectively. Inter-day and intra-day RSD were all below 10.5%. The recoveries were 94.96% for clonidine hydrochloride, 84.45% for hydroehlorothiazide, and 89.88 % for rutin. Conclusion Clonidine hydrochloride, hydrochlorothiazide, and rutin are baseline separated. The method is simple and rapid for simultaneous determination of the three drug components in Zhenju Jiangya tablet. 展开更多
关键词 capillary electrophoresis clonidine hydrochlofide HYDROCHLOROTHIAZIDE RUTIN ZhenjuJiangya tablet
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Optimization of Multiplex PCR and Multiplex Gel Electrophoresis in Sunflower SSR Analysis Using Infrared Fluorescence and Tailed Primers 被引量:3
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作者 张潞生 Vanessa BECQUET +1 位作者 李绍华 David ZHANG 《Acta Botanica Sinica》 CSCD 2003年第11期1312-1318,共7页
In an effort to simplify the procedure and to reduce the cost of fluorescence SSR analysis, the conditions of the multiplex PCR and the multiplex gel electrophoresis were optimized in the genetic analysis of sunflower... In an effort to simplify the procedure and to reduce the cost of fluorescence SSR analysis, the conditions of the multiplex PCR and the multiplex gel electrophoresis were optimized in the genetic analysis of sunflower (Helianthus annuus L.) inbred lines. Results indicated that factors for a successful multiplex PCR assay were related to the cycling touchdown annealing temperature, the balance of primer concentration at the various loci, the concentration of PCR buffer and the Taq DNA polymerase. Based on the optimization, a tailed primer strategy was outlined, and the effective ways were proposed to overcome the troubleshootings commonly encountered in the multiplex PCR and the multiplex gel electrophoresis. 展开更多
关键词 simple sequence repeat (SSR) tailed primer multiplex PCR multiplex gel electrophoresis SUNFLOWER
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A New Capillary Electrophoresis Apparatus with Piezoelectric Ceramics High Voltage Source and Amperometric Detector 被引量:19
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作者 Chen, ZG Mo, JYM +3 位作者 Yang, XY Wang, LS Mei, XT Zhang, MS 《Chinese Chemical Letters》 SCIE CAS CSCD 1999年第3期231-234,共4页
A new capillary electrophoresis apparatus was designed. Piezoelectric ceramics transformer technology was first applied in capillary electrophoresis, a high voltage and stable source was made. Amperometric detector wa... A new capillary electrophoresis apparatus was designed. Piezoelectric ceramics transformer technology was first applied in capillary electrophoresis, a high voltage and stable source was made. Amperometric detector was used in which the working electrode was closely opposite to the end of capillary. The apparatus was characterized in good reproducibility, safety and very low cost. 展开更多
关键词 capillary electrophoresis piezoelectric ceramics amperometric detector
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