Continuous separation of protein mixtures by multichannel flow electrophoresis(MFE)wascarried out in a 5-compartment electrolyzer partitioned by membranes.Polyvinyl alcohol(PVA),polyethylene glycol 4000(PEG 4000)and p...Continuous separation of protein mixtures by multichannel flow electrophoresis(MFE)wascarried out in a 5-compartment electrolyzer partitioned by membranes.Polyvinyl alcohol(PVA),polyethylene glycol 4000(PEG 4000)and polyvinylpyrrolidene K30(PVP K30)were applied to theMFE as shielding polymers to prevent protein adsorption on the polyethersulfone microfiltrationmembrane,which was used to space the central compartment and the elution compartments,duringthe electrophoresis process.The effects of polymer concentration on protein transmembrane flux wereexamined.It was found that PVA,PEG 4000 and PVP K30 greatly reduced protein adsorpton on themembrane surface.Continuous separations of bovine serum albumin(BSA)and hemoglobin(HBB)mix-ture in the presence of PEG 4000 yielded 26.6mg BSA 40.4mg HBB per hour.These results haveshown a high potential of scaling up MFE for large scale separation and purification ofbiomolecules.展开更多
The mechanism of dispersion induced by turn in the capillary electrophoresis channel flows was analyzed firstly. Then the mathematical model of electroosmotic flow is built, and the dispersion of the flow, with differ...The mechanism of dispersion induced by turn in the capillary electrophoresis channel flows was analyzed firstly. Then the mathematical model of electroosmotic flow is built, and the dispersion of the flow, with different distribution of charge at inner and outer wall in the turns, was simulated numerically using the finite differential method. A new approach of altering the distribution of charge at inner and outer wall in the turns was presented, based on the computational results, to minimize the dispersion induced by turn. Meanwhile, an optimization algorithm to analyze the numerical results and determine the optimal distribution of charge in the turns was also developed. It is found that the dispersion induced by turn in the capillary electrophoresis channel flows could be significantly suppressed by this approach.展开更多
Fosfomycin, a sodium salt ofcis-(3-methyloxiranyl) phosphonic acid, was used as electrolyte in binary methanol-water media for capillary electrophoresis. The variety of electroosmotic flow with pH*, methanol concentra...Fosfomycin, a sodium salt ofcis-(3-methyloxiranyl) phosphonic acid, was used as electrolyte in binary methanol-water media for capillary electrophoresis. The variety of electroosmotic flow with pH*, methanol concentration and ionic strength was investigated. The migration behavior of nine bases was examined under various conditions, and the separation of thymine, cytosine, 5-flurouracil, 4, 6-diamino-pyrimidine, purine was accomplished.展开更多
基金This work was supported by the National Natural Science Foundation of China
文摘Continuous separation of protein mixtures by multichannel flow electrophoresis(MFE)wascarried out in a 5-compartment electrolyzer partitioned by membranes.Polyvinyl alcohol(PVA),polyethylene glycol 4000(PEG 4000)and polyvinylpyrrolidene K30(PVP K30)were applied to theMFE as shielding polymers to prevent protein adsorption on the polyethersulfone microfiltrationmembrane,which was used to space the central compartment and the elution compartments,duringthe electrophoresis process.The effects of polymer concentration on protein transmembrane flux wereexamined.It was found that PVA,PEG 4000 and PVP K30 greatly reduced protein adsorpton on themembrane surface.Continuous separations of bovine serum albumin(BSA)and hemoglobin(HBB)mix-ture in the presence of PEG 4000 yielded 26.6mg BSA 40.4mg HBB per hour.These results haveshown a high potential of scaling up MFE for large scale separation and purification ofbiomolecules.
基金Project supported by the National Natural Science Foundation of China (No. 20299030)
文摘The mechanism of dispersion induced by turn in the capillary electrophoresis channel flows was analyzed firstly. Then the mathematical model of electroosmotic flow is built, and the dispersion of the flow, with different distribution of charge at inner and outer wall in the turns, was simulated numerically using the finite differential method. A new approach of altering the distribution of charge at inner and outer wall in the turns was presented, based on the computational results, to minimize the dispersion induced by turn. Meanwhile, an optimization algorithm to analyze the numerical results and determine the optimal distribution of charge in the turns was also developed. It is found that the dispersion induced by turn in the capillary electrophoresis channel flows could be significantly suppressed by this approach.
基金Supported by the National Natural Science Foundation of China!(29875020)
文摘Fosfomycin, a sodium salt ofcis-(3-methyloxiranyl) phosphonic acid, was used as electrolyte in binary methanol-water media for capillary electrophoresis. The variety of electroosmotic flow with pH*, methanol concentration and ionic strength was investigated. The migration behavior of nine bases was examined under various conditions, and the separation of thymine, cytosine, 5-flurouracil, 4, 6-diamino-pyrimidine, purine was accomplished.
基金Supported by the National Natural Science Foundation of China(20275014)Interdisciplinary Innovation Research Fund for Young Scholars,Lanzhou University(824611).
