Study on External Application of Eleutherosides Ointment on Shenque(CV 8)for Resisting Sleep Deprivation

Objective： To investigate the efficacy of external application of Eleutherosides ointment on Shenque （CV 8） for treating sleep deprivation. Methods： Twenty four healthy young male volunteers at the age of 19-22 years old were randomly allocated to 4 groups （normal, model, treatment and control groups）. Sleep was deprived for 48 hours except the normal group. Self-made Eleutherosides compound preparation was applied externally on Shenque （CV 8）, to observe its influence on psychology and blood biochemical indices of cortisol and testosterone of the human body in sleep deprivation. Results： After sleep deprivation for 48 hours, an increment in cortisol concentration was lower in the Eleutherosides group （44.482±96.065 nmol/L） than in the control group （146.809 ±71.075 nmol/L）; an increment in the self-evaluated depression scale was also lower in the Eleutherosides group （2.833 ± 16.746） than in the control group （20.417 ±10.358）. There were significant differences （P〈0.05）. But, there was no significant difference between the treatment group and control group （P〉0.05） in the decrement of testosterone concentration and the increment of the anxiety scale. Conclusion： External application of Eleutherosides preparation on Shenque （CV 8） can regulate the stress reaction level and psychological endurance of the human body and produce an effect to resist sleep deprivation.

ADJUSTING EFFECTS OF APPLICATION OF GARLIC PASTE AT SHNQU (神阙 CV8) ON THE CIRCADIAN RHYTHM OF BODY TEMPERATURE INDUCED BY SLEEP DEPRIVATION

Objective To observe the effect of application of garlic paste at Shenque （神阙 CV8） on the circadian rhythm in sleep deprivation young students. Metheds Twenty healthy volunteer young male students from Southern Medical University were randomly divided into three groups： normal group （A）, sleep deprivation group （B） and treatment group （C）. Volunteers in group B and C received 48 h sleep deprivation （SD）, and in the mean time volunteers in group C were treated by garlic paste at Shenque （神阙 CV8）, while those in group A had no any treatment. The body temperature of all the volunteers was detected at 6：00 am, 12：00 am, 6：00 pm and 0：00 am, respectively, after the treatment. Results The mean body temperature values in group A and C both were highest at 6： 00 pm and lowest at 6： 00 am which had a significant difference in each group （P〈0.01）; in group B, the mean body temperature was highest at 0：00 am and lowestat 6：00 am, no significant difference was found between them （P〉0.05）. Results of cosine analysis showed that in subjects of group B the circadian rhythm of body temperature still kept going well after SD, but the peak amplitude and amplitude of vibration were higher than those of group A, and the acrophase of group B was obviously lower than that of group C and A. The 3 indexes of group C were similar to those of group A, denoting that garlic paste application of Shenque （神阙 CV8） could prevent disorders of circadian rhythm of the body temperature. Conclusion The garlic paste application at Shenque （神阙 CV8） can adjust circadian rhythm and accelerate the recovery processes of circadian rhythm in SD young students.

刺五加总甙穴位贴敷抗睡眠剥夺作用的实验研究

目的:观察刺五加总甙(eleutherosides)穴位贴敷疗法的抗睡眠剥夺(sleep deprivation,SD)作用。方法;用小平台水环境法(flower pot technique)对大鼠进行连续96h快速动眼相(rapid eye movement,REM)睡眠剥夺,以自制刺五加总甙复方制剂于神阙穴处贴敷,观察其对模型动物外观疲劳程度及血乳酸(Lactic Acid,LA)、皮质醇(Cortisol Cor)、睾酮(Testosterone T)等血生化指标的影响。结果:刺五加总甙穴位贴敷组大鼠外观疲劳程度、睡眠剥夺所致血乳酸浓度升高及低睾酮状态均较对照组为轻(P<0.01),血皮质醇含量变化与对照组比较无显著差异(P>0.05)。结论:刺五加总甙穴位贴敷可改善实验动物的疲劳状态,调节机体应激反应水平,具有对抗睡眠剥夺所致疲劳的作用。

刺五加总甙穴位贴敷疗法的抗睡眠剥夺作用实验研究

目的 :观察刺五加总甙穴位贴敷疗法的抗睡眠剥夺作用 ,为研制一种适合于部队应用的外用抗睡眠剥夺制剂提供理论和实验依据。方法 :用小平台水环境法 (flowerpottechnique)对大鼠进行连续 96h快速动眼相睡眠剥夺 ,以自制刺五加总甙复方制剂于神阙穴处贴敷 ,观察其对睡眠剥夺动物外观疲劳程度及血乳酸、皮质醇、睾酮等血液生化指标的影响。结果 :96h睡眠剥夺后 ,刺五加总甙穴位贴敷组大鼠外观疲劳程度较轻 ,血乳酸浓度 (2 .0 2 5± 0 .92 0 )mmol·L- 1低于对照组 (4.85 5± 0 .76 9)mmol·L- 1,血清睾酮含量 (5 .2 4 9± 1.783)nmol·L- 1高于对照组 (3.0 6 1± 2 .0 0 3)nmol·L- 1,差异均有显著性 (P <0 .0 1) ,血清皮质醇含量变化与对照组比较无显著差异(P >0 .0 5 )。结论 :刺五加总甙穴位贴敷可减轻实验动物疲劳状态 ,调节机体应激反应水平 。

刺五加总甙神阙穴贴敷抗睡眠剥夺的研究

目的 观察刺五加总甙神阙穴位贴敷疗法的抗睡眠剥夺作用。方法 健康青年男性（19～22岁）志愿者24名，完全随机分为4组（正常组、模型组、处理组、对照组），除正常组外剥夺全部睡眠48h，以自制刺五加总甙复方制剂于神阙穴处贴敷，观察其对睡眠剥夺人体的心理影响以及对皮质醇、睾酮等血液生化指标的影响。结果 48h睡眠剥夺后，刺五加总甙穴位贴敷组皮质醇浓度增高值（44．482±96．065nmol／L）低于对照组增高值（146．809±71．075nmol／L），自评抑郁量表增加值（2．833±16．746）亦低于对照组增加值（20．417±10．358），差异均具有显著性（P〈0．05），而睾酮浓度降低和焦虑量表增加值，处理组与对照组无明显差异（P〉0．05）。结论 刺五加总甙穴位贴敷可调节机体应激反应水平和心理承受能力，具有对抗睡眠剥夺的作用。

大蒜膏贴敷神阙穴整复人体心率和血压昼夜节律的研究

目的:观察神阙穴外贴大蒜膏对抗睡眠剥夺所致昼夜节律紊乱的整复作用,初步探讨大蒜膏整复昼夜节律紊乱的机理。方法:将20名健康成年男性随机分为三组:正常对照组(A)、睡眠剥夺组(B)、睡眠剥夺并贴药组(C),对B和C组同时进行48h睡眠剥夺,A组不做任何处理,观察A组及睡眠剥夺后B和C组的血压和心率的昼夜变化。结果:A组心率卯时最低,午时最高,二者之间差异有显著性意义(P<0.01),呈现近似昼夜节律变化,峰值为241.654,大约出现在16:11;B组心率卯时最低,子时最高,二者相比没有显著性意义(P>0.05),近似节律消失;C组的心率在卯时最低,酉时最高,二者之间差异有显著性意义(P<0.01),也呈现近似昼夜节律变化,峰值270.744,出现在18:05左右,与A组相比没有出现明显的漂移。三组的收缩压和舒张压都没有近似节律性(P>0.05)。结论:大蒜膏穴位贴敷可以调整人体紊乱的昼夜节律,在一定程度上促进节律的恢复。

刺五加总甙神阙穴贴敷抗睡眠剥夺效果观察

目的：观察刺五加总甙神阙穴位贴敷疗法的抗睡眠剥夺作用。方法：健康青年男性（19～22岁）志愿者24名，完全随机分为正常组、模型组、处理组、对照组4组，除正常组外剥夺全部睡眠48h，以刺五加总甙复方制剂于神阙穴处贴敷，观察其对睡眠剥夺人体的心理影响以及对皮质醇、睾酮等血液生化指标的影响。结果：48h睡眠剥夺后，刺五加总甙穴位贴敷组皮质醇浓度增高值（16．117±34．807ng／dl）低于对照组增高值（53．192±25．752ng／dl），自评抑郁量表增加值（2.833±16．746）亦低于对照组增加值（20．417±10．358），而睾酮浓度降低和焦虑量表增加值与对照组无明显差异。结论：刺五加总甙穴位贴敷可调节机体应激反应水平和心理承受能力。具有对抗睡眠剥夺的作用。

逆针灸联合三才配穴对完全睡眠剥夺24h青年学生疲劳的影响

目的观察逆针灸联合三才配穴对完全睡眠剥夺(TSD)24 h后青年学生疲劳程度的影响。方法将60例健康男性志愿者随机分为治疗组和对照组,各30例。其中治疗组脱落2例。实际入组治疗组28例,对照组30例。治疗组取百会、神门(双)、三阴交(双),即三才配穴,在TSD 24 h前接受7 d的逆针灸预防性治疗,对照组不接受任何治疗,比较TSD 24 h前后受试者的疲劳严重度量表(FSS)评分、简易疲劳量表评分(BFI)和脑电超慢涨落图技术(ET)脑疲劳程度监测值的变化,统计分析差异。结果TSD 24 h后,治疗组的FSS量表评分、BFI量表评分均低于对照组,有显著性差异(均P<0.05),治疗组脑疲劳状态监测值低于对照组,有极显著性差异(P<0.01)。结论逆针灸联合三才配穴对青年学生TSD 24 h后产生的疲劳感具有缓解作用。