Two-line hybrid rice with excellent quality is preferred in the Chinese market.However,there is a trade-off between reducing costs for hybrid seed production and lowering the outcrossing rate of the sterile line,which...Two-line hybrid rice with excellent quality is preferred in the Chinese market.However,there is a trade-off between reducing costs for hybrid seed production and lowering the outcrossing rate of the sterile line,which is largely determined by the stigma exsertion rate(SER).In this study,we constructed mutants of male sterility lines with improved grain length(GL)and SER in three elite early-season indica rice varieties through targeted manipulation of the TMS5 and GS3 genes using CRISPR/Cas9-mediated multiplex systems.We obtained a series of marker-free gs3 single mutants and gs3tms5 double mutants with significantly higher SER,longer grains,and increased 1000-grain weight compared with the wild type(WT).Importantly,the typically thermo-sensitive genic male sterile(TGMS)trait with a higher SER was observed in gs3tms5 mutants,and their F1 hybrids exhibited remarkable improvements in grain shape and yield-related traits.Our findings provided an efficient method to generate new valuable TGMS germplasm with improved SER through the mutagenesis of GS3 and TMS5 synergistically,and demonstrated that GS3 had pleiotropic effects on grain size,SER,and grain quality in early-season indica rice.展开更多
This study analyzed the difference between using a downward breaststroke kick and a horizontal breaststroke kick in a sample of world class elite swimmers.We compared average muscle activity of the gluteus maximus,qua...This study analyzed the difference between using a downward breaststroke kick and a horizontal breaststroke kick in a sample of world class elite swimmers.We compared average muscle activity of the gluteus maximus,quadriceps femoris(vastus medialis and rectus femoris),hamstring/long head of the biceps femoris,gastrocnemius medialis,rectus abdominal,and erector spinae when using the downward breaststroke kick technique.We find that when this sample of swimmers utilized the downward breaststroke kick,max speed and velocity per stroke increased,measured by 12,788 EMG samples,where the results are highly correlated to duration of the aerodynamic buoyant force in breaststroke kick technique.The increases in performance observed from measuring the world class elite swimmers is highly correlated to the duration of the kick aerodynamic buoyant force.Among this sample of elite swimmers,the longer a swimmer demonstrates a buoyant force breaststroke kick,the lower the time in a 100 breaststroke.展开更多
Many young elite athletes do not meet their daily energy and nutrient requirements. However, little research has been done on why these athletes do not meet their daily needs. The aim was to research the barriers and ...Many young elite athletes do not meet their daily energy and nutrient requirements. However, little research has been done on why these athletes do not meet their daily needs. The aim was to research the barriers and motivators of young Dutch elite athletes to optimize their nutritional intake. Quantitative and qualitative research was conducted among 8 handball and 4 volleyball players at the Dutch National Sports Center (17.2 ± 0.8 years). First, the nutritional intake was tracked through food diaries and analyzed in Nutritics. Thereupon, five semi-structured interviews based on the COM-B model were carried out. The interviews were transcribed and coded. The athletes had a reduced intake of energy, carbohydrates, vitamins A, C, E, D, calcium, potassium, zinc, and iron compared to their requirements. Seven themes for optimizing their nutritional intake emerged in the interviews: needs assessment, practical translation, portion size, lack of time, involvement, individuality, and food distribution. Barriers that the athletes experienced were that they did not know what their total daily nutritional needs were and how this translates into practice. In addition, the portion size at dinner was too small. They also had little time to eat a full meal due to time pressure from training and school. On the other hand, motivators were receiving meal options to translate their needs into practice with a distribution of moments when they need to eat. Covering these topics in nutritional workshops where athletes actively participate with more individual focus, could contribute to the optimization of their nutritional intake.展开更多
Indonesian Minister of Defence Prabowo Subianto won Indonesia’s presidential election in February 2024.Prabowo hails from an elite family that has served in many high positions in Indonesia.Having served in important...Indonesian Minister of Defence Prabowo Subianto won Indonesia’s presidential election in February 2024.Prabowo hails from an elite family that has served in many high positions in Indonesia.Having served in important positions in the military for a long time,he is very well connected with military and political circles.展开更多
Due to the complexity of leadership and its variability across situations,it is suggested that research on leadership should focus on attempts to understand leadership in specific situations or roles,and develop local...Due to the complexity of leadership and its variability across situations,it is suggested that research on leadership should focus on attempts to understand leadership in specific situations or roles,and develop local knowledge about leadership in each situation or role.This paper validates the possibility and importance of building indigenous leadership theories from historical perspective.We demonstrated how to carry out research on indigenous Chinese leadership from a historical perspective by comparative case studies using historical data.Based on the principles of typicalness,representativeness,and comparativeness,we selected six counterparts of political elites in ancient China as comparative cases,which includes Guan Zhong&Shang Yang(organizational reformer),Liu Bang&Xiang Yu(entrepreneur),Li Shimin&Qin Shihuang(intergenerational transition successor),Zhuge Liang&Zeng Guofan(professional manager),Lu Su&Zhang Juzheng(mess-to-order maker),and Zuo Zongtang&Wang Anshi(national dream driver).The paper aims to develop indigenous leadership theory specific to Chinese mental states and culture.展开更多
The performance of proton exchange membrane fuel cells is very sensitive to temperature. The electrochemical reaction results directly in temperature variations in the proton exchange membrane fuel cell. Ensuring effe...The performance of proton exchange membrane fuel cells is very sensitive to temperature. The electrochemical reaction results directly in temperature variations in the proton exchange membrane fuel cell. Ensuring effective temperature control is crucial to ensure fuel cell reliability and durability. This paper uses active disturbance rejection control in the thermal management system to maintain the operating temperature and the stack inlet and outlet temperature difference at the set value. First, key cooling system modules such as expansion tanks, coolant circulation pumps and radiators based on Simulink were built. Then, physical modeling and simulation of the fuel cell cooling system was carried out. In order to ensure the effectiveness of the control strategy and reduce the parameter tuning workload, an active disturbance rejection control parameter optimization method using an elite genetic algorithm was proposed. When the optimized control strategy responds to input disturbances, the maximum overshoot of the system is only 1.23% and can reach stability again in 30 s, so the fuel cell temperature can be controlled effectively. Simulation results show that the optimized control strategy can effectively control the stack temperature and coolant temperature difference under the influence of stepped charging current without interference or with interference, and has strong robustness and anti-interference capability.展开更多
Melon is an important horticultural crop with extensive diversity in many horticultural groups.To explore its genomic diversity,it is necessary to assemble more high-quality complete genomes from different melon acces...Melon is an important horticultural crop with extensive diversity in many horticultural groups.To explore its genomic diversity,it is necessary to assemble more high-quality complete genomes from different melon accessions.Meanwhile,a large number of QTLs have been mapped in several studies.Integration of the published QTLs onto a complete genome can provide more accurate information for candidate gene cloning.To address these problems,a telomere-to-telomere(T2T)genome of the elite melon landrace Kuizilikjiz(Cucumis melo L.var.inodorus)was de novo assembled and all the published QTLs were projected onto it in this study.The results showed that a high-quality Kuizilikjiz genome with the size of 379.2 Mb and N50 of 31.7 Mb was de novo assembled using the combination of short reads,PacBio high-fidelity long reads,Hi-C data,and a high-density genetic map.Each chromosome contained the centromere and telomeres at both ends.A large number of structural variations were observed between Kuizilikjiz and the other published genomes.A total of 1294 QTLs published in 67 studies were collected and projected onto the T2T genome.Several clustered,co-localized,and overlapped QTLs were determined.Furthermore,20 stable meta-QTLs were identified,which significantly reduced the mapping intervals of the initial QTLs and greatly facilitated identification of the candidate genes.Collectively,the T2T genome assembly together with the numerous projected QTLs will not only broaden the high-quality genome resources but also provide valuable and abundant QTL information for cloning the genes controlling important traits in melon.展开更多
Efficient speed controllers for dynamic driving tasks in autonomous vehicles are crucial for ensuring safety and reliability.This study proposes a novel approach for designing a fractional order proportional-integral-...Efficient speed controllers for dynamic driving tasks in autonomous vehicles are crucial for ensuring safety and reliability.This study proposes a novel approach for designing a fractional order proportional-integral-derivative(FOPID)controller that utilizes a modified elite opposition-based artificial hummingbird algorithm(m-AHA)for optimal parameter tuning.Our approach outperforms existing optimization techniques on benchmark functions,and we demonstrate its effectiveness in controlling cruise control systems with increased flexibility and precision.Our study contributes to the advancement of autonomous vehicle technology by introducing a novel and efficient method for FOPID controller design that can enhance the driving experience while ensuring safety and reliability.We highlight the significance of our findings by demonstrating how our approach can improve the performance,safety,and reliability of autonomous vehicles.This study’s contributions are particularly relevant in the context of the growing demand for autonomous vehicles and the need for advanced control techniques to ensure their safe operation.Our research provides a promising avenue for further research and development in this area.展开更多
Background:Developing context-specific,evidence-informed,and implementable injury-prevention programs is challenging.Women playing in the elite Australian Football League for Women are at high risk of serious knee inj...Background:Developing context-specific,evidence-informed,and implementable injury-prevention programs is challenging.Women playing in the elite Australian Football League for Women are at high risk of serious knee injuries,and no specific injury-prevention program exists.The objective of the study was to describe the collaborative process used to create a context-specific injury-prevention program.Methods:A previously used intervention-development process was modified to incorporate a partnership with the sport’s governing organization and focus on engaging program implementers.The Reach Effectiveness Adoption Implementation and Maintenance(RE-AIM)Sports Setting Matrix guided program development and implementation strategies.Results:The 7-step process,aligned with the RE-AIM Sports Setting Matrix,was applied to develop the injury-prevention program and was titled Prep to Play PRO.The steps were:(Step 1)gaining organizational support and establishing a project partnership;(Step 2)using research evidence and clinical experience;(Step 3)consulting content and context experts;(Step 4)engaging the organization,experts,program implementers,and end-users to concreate the intervention and develop implementation strategies;(Step 5)testing the intervention acceptability and feasibility;(Step 6)evaluating the intervention and implementation strategies against theory;and(Step 7)obtaining feedback from early implementers and end-users.Conclusion:Engaging critical stakeholders at multiple ecological levels(organization,team,and athlete)throughout program development and implementation planning support real-world use.The processes and activities described can guide future sports injury-prevention program development and implementation.展开更多
Rice panicle branches are key routes for transportation of photo synthetic products to grains.Branch senescence is an important trait that the breeders considered when breeding elite rice cultivars in South China.Howe...Rice panicle branches are key routes for transportation of photo synthetic products to grains.Branch senescence is an important trait that the breeders considered when breeding elite rice cultivars in South China.However,little is known about the mechanisms underlying the contributions of stay-green panicle branches to rice yield and quality.Here,we reported stay-green branches that continuously maintained transportation function and provided carbohydrates for basal grain development at the late stages of rice development.This ensured the coordination of development between apical and basal grains,and further improved the processing quality of elite rice.These findings provided strategies for improving the fullness and quality of rice grains and presented an important basis for cultivating elite rice.展开更多
The disintegration of networks is a widely researched topic with significant applications in fields such as counterterrorism and infectious disease control. While the traditional approaches for achieving network disin...The disintegration of networks is a widely researched topic with significant applications in fields such as counterterrorism and infectious disease control. While the traditional approaches for achieving network disintegration involve identifying critical sets of nodes or edges, limited research has been carried out on edge-based disintegration strategies. We propose a novel algorithm, i.e., a rank aggregation elite enumeration algorithm based on edge-coupled networks(RAEEC),which aims to implement tiling for edge-coupled networks by finding important sets of edges in the network while balancing effectiveness and efficiency. Our algorithm is based on a two-layer edge-coupled network model with one-to-one links, and utilizes three advanced edge importance metrics to rank the edges separately. A comprehensive ranking of edges is obtained using a rank aggregation approach proposed in this study. The top few edges from the ranking set obtained by RAEEC are then used to generate an enumeration set, which is continuously iteratively updated to identify the set of elite attack edges.We conduct extensive experiments on synthetic networks to evaluate the performance of our proposed method, and the results indicate that RAEEC achieves a satisfactory balance between efficiency and effectiveness. Our approach represents a significant contribution to the field of network disintegration, particularly for edge-based strategies.展开更多
Grape white rot is a disease caused by Coniella diplodiella(Speg.)Sacc.(Cd)can drastically reduce the production and quality of grape(Vitis vinifera).WRKY transcription factors play a vital role in the regulation of p...Grape white rot is a disease caused by Coniella diplodiella(Speg.)Sacc.(Cd)can drastically reduce the production and quality of grape(Vitis vinifera).WRKY transcription factors play a vital role in the regulation of plant resistance to pathogens,but their functions in grape white rot need to be further explored.Here,we found that the expression of the WRKY IIe subfamily member VvWRKY5 was highly induced by Cd infection and jasmonic acid(JA)treatment.Transient injection and stable overexpression(in grape calli and Arabidopsis)demonstrated that VvWRKY5 positively regulated grape resistance to white rot.We also determined that VvWRKY5 regulated the JA response by directly binding to the promoters of VvJAZ2(a JA signaling suppressor)and VvMYC2(a JA signaling activator),thereby inhibiting and activating the transcription of VvJAZ2 and VvMYC2,respectively.Furthermore,the interaction between VvJAZ2 and VvWRKY5 enhanced the suppression and promotion of VvJAZ2 and VvMYC2 activities by VvWRKY5,respectively.When VvWRKY5 was overexpressed in grape,JA content was also increased.Overall,our results suggested that VvWRKY5 played a key role in regulating JA biosynthesis and signal transduction as well as enhancing white rot resistance in grape.Our results also provide theoretical guidance for the development of elite grape cultivars with enhanced pathogen resistance.展开更多
No optimization algorithm can obtain satisfactory results in all optimization tasks.Thus,it is an effective way to deal with the problem by an ensemble ofmultiple algorithms.This paper proposes an ensemble of populati...No optimization algorithm can obtain satisfactory results in all optimization tasks.Thus,it is an effective way to deal with the problem by an ensemble ofmultiple algorithms.This paper proposes an ensemble of population-based metaheuristics(EPM)to solve single-objective optimization problems.The design of the EPM framework includes three stages:the initial stage,the update stage,and the final stage.The framework applies the transformation of the real and virtual population to balance the problem of exploration and exploitation at the population level and uses an elite strategy to communicate among virtual populations.The experiment tested two benchmark function sets with fivemetaheuristic algorithms and four ensemble algorithms.The ensemble algorithms are generally superior to the original algorithms by Friedman’s average ranking andWilcoxon signed ranking test results,demonstrating the ensemble framework’s effect.By solving the iterative curves of different test functions,we can see that the ensemble algorithms have faster iterative optimization speed and better optimization results.The ensemble algorithms cannot fall into local optimumby virtual populations distribution map of several stages.The ensemble framework performs well from the effects of solving two practical engineering problems.Some results of ensemble algorithms are superior to those of metaheuristic algorithms not included in the ensemble framework,further demonstrating the ensemble method’s potential and superiority.展开更多
基金the Natural Science Foundation of Zhejiang Province,China(Grant Nos.LY24C130004,LY22C135104,and LY23C130002)the National Natural Science Foundation of China(Grant No.31501288)+1 种基金the Open Project Program of State Key Laboratory of Rice Biology and Breeding,China(Grant No.20210207)Central Publicinterest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRF-CNRRI-202203).
文摘Two-line hybrid rice with excellent quality is preferred in the Chinese market.However,there is a trade-off between reducing costs for hybrid seed production and lowering the outcrossing rate of the sterile line,which is largely determined by the stigma exsertion rate(SER).In this study,we constructed mutants of male sterility lines with improved grain length(GL)and SER in three elite early-season indica rice varieties through targeted manipulation of the TMS5 and GS3 genes using CRISPR/Cas9-mediated multiplex systems.We obtained a series of marker-free gs3 single mutants and gs3tms5 double mutants with significantly higher SER,longer grains,and increased 1000-grain weight compared with the wild type(WT).Importantly,the typically thermo-sensitive genic male sterile(TGMS)trait with a higher SER was observed in gs3tms5 mutants,and their F1 hybrids exhibited remarkable improvements in grain shape and yield-related traits.Our findings provided an efficient method to generate new valuable TGMS germplasm with improved SER through the mutagenesis of GS3 and TMS5 synergistically,and demonstrated that GS3 had pleiotropic effects on grain size,SER,and grain quality in early-season indica rice.
文摘This study analyzed the difference between using a downward breaststroke kick and a horizontal breaststroke kick in a sample of world class elite swimmers.We compared average muscle activity of the gluteus maximus,quadriceps femoris(vastus medialis and rectus femoris),hamstring/long head of the biceps femoris,gastrocnemius medialis,rectus abdominal,and erector spinae when using the downward breaststroke kick technique.We find that when this sample of swimmers utilized the downward breaststroke kick,max speed and velocity per stroke increased,measured by 12,788 EMG samples,where the results are highly correlated to duration of the aerodynamic buoyant force in breaststroke kick technique.The increases in performance observed from measuring the world class elite swimmers is highly correlated to the duration of the kick aerodynamic buoyant force.Among this sample of elite swimmers,the longer a swimmer demonstrates a buoyant force breaststroke kick,the lower the time in a 100 breaststroke.
文摘Many young elite athletes do not meet their daily energy and nutrient requirements. However, little research has been done on why these athletes do not meet their daily needs. The aim was to research the barriers and motivators of young Dutch elite athletes to optimize their nutritional intake. Quantitative and qualitative research was conducted among 8 handball and 4 volleyball players at the Dutch National Sports Center (17.2 ± 0.8 years). First, the nutritional intake was tracked through food diaries and analyzed in Nutritics. Thereupon, five semi-structured interviews based on the COM-B model were carried out. The interviews were transcribed and coded. The athletes had a reduced intake of energy, carbohydrates, vitamins A, C, E, D, calcium, potassium, zinc, and iron compared to their requirements. Seven themes for optimizing their nutritional intake emerged in the interviews: needs assessment, practical translation, portion size, lack of time, involvement, individuality, and food distribution. Barriers that the athletes experienced were that they did not know what their total daily nutritional needs were and how this translates into practice. In addition, the portion size at dinner was too small. They also had little time to eat a full meal due to time pressure from training and school. On the other hand, motivators were receiving meal options to translate their needs into practice with a distribution of moments when they need to eat. Covering these topics in nutritional workshops where athletes actively participate with more individual focus, could contribute to the optimization of their nutritional intake.
文摘Indonesian Minister of Defence Prabowo Subianto won Indonesia’s presidential election in February 2024.Prabowo hails from an elite family that has served in many high positions in Indonesia.Having served in important positions in the military for a long time,he is very well connected with military and political circles.
文摘Due to the complexity of leadership and its variability across situations,it is suggested that research on leadership should focus on attempts to understand leadership in specific situations or roles,and develop local knowledge about leadership in each situation or role.This paper validates the possibility and importance of building indigenous leadership theories from historical perspective.We demonstrated how to carry out research on indigenous Chinese leadership from a historical perspective by comparative case studies using historical data.Based on the principles of typicalness,representativeness,and comparativeness,we selected six counterparts of political elites in ancient China as comparative cases,which includes Guan Zhong&Shang Yang(organizational reformer),Liu Bang&Xiang Yu(entrepreneur),Li Shimin&Qin Shihuang(intergenerational transition successor),Zhuge Liang&Zeng Guofan(professional manager),Lu Su&Zhang Juzheng(mess-to-order maker),and Zuo Zongtang&Wang Anshi(national dream driver).The paper aims to develop indigenous leadership theory specific to Chinese mental states and culture.
文摘The performance of proton exchange membrane fuel cells is very sensitive to temperature. The electrochemical reaction results directly in temperature variations in the proton exchange membrane fuel cell. Ensuring effective temperature control is crucial to ensure fuel cell reliability and durability. This paper uses active disturbance rejection control in the thermal management system to maintain the operating temperature and the stack inlet and outlet temperature difference at the set value. First, key cooling system modules such as expansion tanks, coolant circulation pumps and radiators based on Simulink were built. Then, physical modeling and simulation of the fuel cell cooling system was carried out. In order to ensure the effectiveness of the control strategy and reduce the parameter tuning workload, an active disturbance rejection control parameter optimization method using an elite genetic algorithm was proposed. When the optimized control strategy responds to input disturbances, the maximum overshoot of the system is only 1.23% and can reach stability again in 30 s, so the fuel cell temperature can be controlled effectively. Simulation results show that the optimized control strategy can effectively control the stack temperature and coolant temperature difference under the influence of stepped charging current without interference or with interference, and has strong robustness and anti-interference capability.
基金This work was supported by the Key R&D Project of Hubei Province(2021BBA101)the Fundamental Research Funds for the Central Universities(2662020YLPY024)+2 种基金the Key R&D Project of Xinjiang Academy of Agricultural Sciences(xjkcpy-2022006)the Tianshan Innovation Team(2022D14015)the China Agriculture Research System(CARS-25).
文摘Melon is an important horticultural crop with extensive diversity in many horticultural groups.To explore its genomic diversity,it is necessary to assemble more high-quality complete genomes from different melon accessions.Meanwhile,a large number of QTLs have been mapped in several studies.Integration of the published QTLs onto a complete genome can provide more accurate information for candidate gene cloning.To address these problems,a telomere-to-telomere(T2T)genome of the elite melon landrace Kuizilikjiz(Cucumis melo L.var.inodorus)was de novo assembled and all the published QTLs were projected onto it in this study.The results showed that a high-quality Kuizilikjiz genome with the size of 379.2 Mb and N50 of 31.7 Mb was de novo assembled using the combination of short reads,PacBio high-fidelity long reads,Hi-C data,and a high-density genetic map.Each chromosome contained the centromere and telomeres at both ends.A large number of structural variations were observed between Kuizilikjiz and the other published genomes.A total of 1294 QTLs published in 67 studies were collected and projected onto the T2T genome.Several clustered,co-localized,and overlapped QTLs were determined.Furthermore,20 stable meta-QTLs were identified,which significantly reduced the mapping intervals of the initial QTLs and greatly facilitated identification of the candidate genes.Collectively,the T2T genome assembly together with the numerous projected QTLs will not only broaden the high-quality genome resources but also provide valuable and abundant QTL information for cloning the genes controlling important traits in melon.
文摘Efficient speed controllers for dynamic driving tasks in autonomous vehicles are crucial for ensuring safety and reliability.This study proposes a novel approach for designing a fractional order proportional-integral-derivative(FOPID)controller that utilizes a modified elite opposition-based artificial hummingbird algorithm(m-AHA)for optimal parameter tuning.Our approach outperforms existing optimization techniques on benchmark functions,and we demonstrate its effectiveness in controlling cruise control systems with increased flexibility and precision.Our study contributes to the advancement of autonomous vehicle technology by introducing a novel and efficient method for FOPID controller design that can enhance the driving experience while ensuring safety and reliability.We highlight the significance of our findings by demonstrating how our approach can improve the performance,safety,and reliability of autonomous vehicles.This study’s contributions are particularly relevant in the context of the growing demand for autonomous vehicles and the need for advanced control techniques to ensure their safe operation.Our research provides a promising avenue for further research and development in this area.
基金supported by an AFL Research Board Grant(2018)La Trobe University Research Focus Area Grant(2018)+2 种基金La Trobe University Exercise and Medicine Research Centresupported by a National Health and Medical Research Council(NHMRC)Early Career Fellowship(No.1156674)supported by a NHMRC Postgraduate Scholarship(No.1114296).
文摘Background:Developing context-specific,evidence-informed,and implementable injury-prevention programs is challenging.Women playing in the elite Australian Football League for Women are at high risk of serious knee injuries,and no specific injury-prevention program exists.The objective of the study was to describe the collaborative process used to create a context-specific injury-prevention program.Methods:A previously used intervention-development process was modified to incorporate a partnership with the sport’s governing organization and focus on engaging program implementers.The Reach Effectiveness Adoption Implementation and Maintenance(RE-AIM)Sports Setting Matrix guided program development and implementation strategies.Results:The 7-step process,aligned with the RE-AIM Sports Setting Matrix,was applied to develop the injury-prevention program and was titled Prep to Play PRO.The steps were:(Step 1)gaining organizational support and establishing a project partnership;(Step 2)using research evidence and clinical experience;(Step 3)consulting content and context experts;(Step 4)engaging the organization,experts,program implementers,and end-users to concreate the intervention and develop implementation strategies;(Step 5)testing the intervention acceptability and feasibility;(Step 6)evaluating the intervention and implementation strategies against theory;and(Step 7)obtaining feedback from early implementers and end-users.Conclusion:Engaging critical stakeholders at multiple ecological levels(organization,team,and athlete)throughout program development and implementation planning support real-world use.The processes and activities described can guide future sports injury-prevention program development and implementation.
基金supported by the Laboratory of Lingnan Modern Agriculture Project,China (Grant No. NT2021001)the National Natural Science Foundation of China (Grant No. 31801448)+1 种基金the Special Fund for Scientific Innovation Strategy-Construction of High Level Academy of Agriculture Science,China (Grant No. R2018PY-QF003)the Special Fund for Science and Technology Innovation Strategy of Guangdong Academy of Agricultural Sciences,Dean’s Fund Key Project (Grant No. 202001)。
文摘Rice panicle branches are key routes for transportation of photo synthetic products to grains.Branch senescence is an important trait that the breeders considered when breeding elite rice cultivars in South China.However,little is known about the mechanisms underlying the contributions of stay-green panicle branches to rice yield and quality.Here,we reported stay-green branches that continuously maintained transportation function and provided carbohydrates for basal grain development at the late stages of rice development.This ensured the coordination of development between apical and basal grains,and further improved the processing quality of elite rice.These findings provided strategies for improving the fullness and quality of rice grains and presented an important basis for cultivating elite rice.
基金supported by the National Natural Science Foundation of China (Grant Nos. 61877046, 12271419, and 62106186)the Natural Science Basic Research Program of Shaanxi (Program No. 2022JQ-620)the Fundamental Research Funds for the Central Universities (Grant Nos. XJS220709, JB210701, and QTZX23002)。
文摘The disintegration of networks is a widely researched topic with significant applications in fields such as counterterrorism and infectious disease control. While the traditional approaches for achieving network disintegration involve identifying critical sets of nodes or edges, limited research has been carried out on edge-based disintegration strategies. We propose a novel algorithm, i.e., a rank aggregation elite enumeration algorithm based on edge-coupled networks(RAEEC),which aims to implement tiling for edge-coupled networks by finding important sets of edges in the network while balancing effectiveness and efficiency. Our algorithm is based on a two-layer edge-coupled network model with one-to-one links, and utilizes three advanced edge importance metrics to rank the edges separately. A comprehensive ranking of edges is obtained using a rank aggregation approach proposed in this study. The top few edges from the ranking set obtained by RAEEC are then used to generate an enumeration set, which is continuously iteratively updated to identify the set of elite attack edges.We conduct extensive experiments on synthetic networks to evaluate the performance of our proposed method, and the results indicate that RAEEC achieves a satisfactory balance between efficiency and effectiveness. Our approach represents a significant contribution to the field of network disintegration, particularly for edge-based strategies.
基金We thank Ying Zhang from Zhengzhou Fruit Research Institute for providing the Coniella diplodiella strain WR01.We thank Haifeng Jia from Nanjing Agricultural University for providing the‘Gamay’grape calli.The research was supported by the National Natural Science Foundation of China(grant no.31972368)the China Agriculture Research System(grant no.CARS-29-yc-6)+2 种基金the Basic research innovation capability enhancement project of provin-cial colleges and universities(grant no.2021JK06)the Depart-ment of Science and Technology of Liaoning Province(grant no.2022030723-JH5/104)the Shenyang Science and Technology Bureau Funds(grant no.21-116-3-27).
文摘Grape white rot is a disease caused by Coniella diplodiella(Speg.)Sacc.(Cd)can drastically reduce the production and quality of grape(Vitis vinifera).WRKY transcription factors play a vital role in the regulation of plant resistance to pathogens,but their functions in grape white rot need to be further explored.Here,we found that the expression of the WRKY IIe subfamily member VvWRKY5 was highly induced by Cd infection and jasmonic acid(JA)treatment.Transient injection and stable overexpression(in grape calli and Arabidopsis)demonstrated that VvWRKY5 positively regulated grape resistance to white rot.We also determined that VvWRKY5 regulated the JA response by directly binding to the promoters of VvJAZ2(a JA signaling suppressor)and VvMYC2(a JA signaling activator),thereby inhibiting and activating the transcription of VvJAZ2 and VvMYC2,respectively.Furthermore,the interaction between VvJAZ2 and VvWRKY5 enhanced the suppression and promotion of VvJAZ2 and VvMYC2 activities by VvWRKY5,respectively.When VvWRKY5 was overexpressed in grape,JA content was also increased.Overall,our results suggested that VvWRKY5 played a key role in regulating JA biosynthesis and signal transduction as well as enhancing white rot resistance in grape.Our results also provide theoretical guidance for the development of elite grape cultivars with enhanced pathogen resistance.
基金supported by National Natural Science Foundation of China under Grant 62073330.The auther J.T.received the grant。
文摘No optimization algorithm can obtain satisfactory results in all optimization tasks.Thus,it is an effective way to deal with the problem by an ensemble ofmultiple algorithms.This paper proposes an ensemble of population-based metaheuristics(EPM)to solve single-objective optimization problems.The design of the EPM framework includes three stages:the initial stage,the update stage,and the final stage.The framework applies the transformation of the real and virtual population to balance the problem of exploration and exploitation at the population level and uses an elite strategy to communicate among virtual populations.The experiment tested two benchmark function sets with fivemetaheuristic algorithms and four ensemble algorithms.The ensemble algorithms are generally superior to the original algorithms by Friedman’s average ranking andWilcoxon signed ranking test results,demonstrating the ensemble framework’s effect.By solving the iterative curves of different test functions,we can see that the ensemble algorithms have faster iterative optimization speed and better optimization results.The ensemble algorithms cannot fall into local optimumby virtual populations distribution map of several stages.The ensemble framework performs well from the effects of solving two practical engineering problems.Some results of ensemble algorithms are superior to those of metaheuristic algorithms not included in the ensemble framework,further demonstrating the ensemble method’s potential and superiority.