[Objective] The aim was to provide scientific basis for development and utilization of Pinus elliottii Engelm resources.[Method] The extraction process of shikimic acid in Pinus elliottii Engelm was studied,and the co...[Objective] The aim was to provide scientific basis for development and utilization of Pinus elliottii Engelm resources.[Method] The extraction process of shikimic acid in Pinus elliottii Engelm was studied,and the content of shikimic acid was determined by HPLC.The HPLC conditions were as follows;Alltima NH2 （5 μm,4.6 mm × 150 mm） column separation;the mobile phase was acetonitrile-2%H3PO4 （90：10）;the flowing velocity was 1 ml/min;test wavelength was 213 nm,the width of belt was 16 nm;reference wavelength was 300 nm,the width of belt was 80 nm.[Result] By the single factor and orthogonal tests,the optimum conditions were found as follows：ethanol concentration 60%,extraction temperature 75 ℃,solid-liquid ratio 1：25,extraction time 2.5 h.The extraction rate of shikimic acid was 1.49%.[Conclusion] Shikimic acid in Pinus elliottii Engelm could be used as a new resource to develop and utilize.展开更多
人类活动引起陆地生态系统氮输入水平持续升高,对全球碳循环产生影响。为探究氮素对土壤呼吸的影响,2010年6月至2012年1月,采用氮添加试验对亚热带湿地松林的土壤呼吸进行了研究。试验共设置4种氮添加水平:对照CK,0 g m^(-2)a^(-1);低氮...人类活动引起陆地生态系统氮输入水平持续升高,对全球碳循环产生影响。为探究氮素对土壤呼吸的影响,2010年6月至2012年1月,采用氮添加试验对亚热带湿地松林的土壤呼吸进行了研究。试验共设置4种氮添加水平:对照CK,0 g m^(-2)a^(-1);低氮LN,5g m^(-2)a^(-1);中氮MN,15 g m^(-2)a^(-1);高氮HN,30 g m^(-2)a^(-1);每月上、下旬采用Li-cor 8100测定土壤呼吸速率。结果表明:(1)氮添加对土壤呼吸有着显著的抑制作用,LN、MN和HN处理的土壤呼吸年累积量较CK处理分别降低了26.6%、23.7%和29.5%,而施氮处理间的土壤呼吸无显著差异;(2)林分生长期间(6-9月),施氮第1年的土壤呼吸所受抑制作用显著高于第2年同期的水平,显示施氮对土壤呼吸的影响随时间的推移而降低;(3)湿地松林土壤呼吸存在明显的季节动态,最大值出现在8月(356.32 mgCO_2 m^(-2)h^(-1)),最小值出现在1月(99.12 mg CO_2 m^(-2)h^(-1)),施氮处理并不改变土壤呼吸的季节性变化规律;(4)CK处理林分中,土壤呼吸与土壤温度间存在极显著的指数关系,而与土壤湿度无显著相关。施氮处理没有改变土壤呼吸与土壤温度之间的相互关系,但抑制了土壤呼吸的温度敏感性(Q_(10));(5)施氮处理显著减少了林分凋落物量、土壤微生物碳、氮量,并轻微抑制了细根生物量,这些改变导致了土壤呼吸的下降。上述结果表明施氮会显著抑制亚热带湿地松林的土壤呼吸速率,而这种抑制作用将随着时间的推移而降低。展开更多
[Objective] The research aimed to study the mycorrhizal formation in the offspring tissue culture seedling bottle of Pinus elliottii with Lecanosticta acicola resistance.[Method] The mycorrhizal formation of Pinus ell...[Objective] The research aimed to study the mycorrhizal formation in the offspring tissue culture seedling bottle of Pinus elliottii with Lecanosticta acicola resistance.[Method] The mycorrhizal formation of Pinus elliottii regenerated plant in vitro was studied,and the growth condition of regenerated plant was observed.[Result] The culture substrate and the inoculation amount of mycorrhizal fungi had the big influence on the mycorrhizal formation in vitro.After the root primordia were induced by Pinus elliottii caespitose shoots,it was best to transplant in the perlite substrate and inoculate Pisolithus tinctorius,which was favorable for the mycorrhizal formation.When the two blocks of Pisolithus tinctorius were inoculated in the perlite medium,the mycorrhizal formation rate reached 84.4%.The dichotomous branching short root was the most,and 12.49 roots were formed in every main root.The mycorrhizal formation improved the survival rate of regenerated plant domestication transplantation.The mycorrhizal regenerated plant grew well in the phytotron,and the root system was developed.[Conclusion] The research provided the basis for improving the survival rate of Pinus elliottii tissue culture regenerated plant with Lecanosticta acicola resistance.展开更多
The development of needle resin ducts of Pinus elliottii was studied using electron microscope. The results indicate that the resin duct development encompasses at least three distinctive development stages: the s...The development of needle resin ducts of Pinus elliottii was studied using electron microscope. The results indicate that the resin duct development encompasses at least three distinctive development stages: the stage of initial cells, the stage of middle lamellae expansion and intercellular space appearance, and the stage of cavity enlargement and the resin duct formation. During the whole development stages, all kinds of organells in the duct cells show different ultrastructural features and regularity, among them the plastids' changing is the most striking one. On the basis of analysing the ultrastructural features of developing resin duct cells, the pattern of duct cavity formation, possible sites of resin synthesis, the manner and way of the resin elimination from the protoplast are discussed.展开更多
Different ground mulching treatments were designed to observe the effects on soil moisture content in a second-generation seed orchard of Pinus elliottii. The results showed that： （1） there were extreme differences...Different ground mulching treatments were designed to observe the effects on soil moisture content in a second-generation seed orchard of Pinus elliottii. The results showed that： （1） there were extreme differences in soil moisture content Ⅰ and soil moisture content Ⅲ, but no obvious difference in soil moisture content II was observed; and （2） the high-to-low order of soil moisture content Ⅰ under different ground mulching treatments was white film〉sawdust〉black film〉the control, and the highest value was higher than the lowest one by 26.69%; the high-to-low order of soil moisture content Ⅱ under different ground mulching treatments was white film〉black film〉sawdust〉the control, and the highest value was higher than the lowest one by 20.64%; and the high-to-low order of soil moisture content Ⅲ under different ground mulching treatments was black film〉white film〉sawdust〉the control, and the highest value was higher than the lowest one by 26.61%.展开更多
基金Supported by National Natural Science Foundation of China(30560119)Supported by Natural Science Foundation of Guangxi(0991030)+3 种基金Innovational Plan Project for Guangxi Postgraduate Ed-ucation(2008105930817M46)Project of Science Research Project of Guangxi Education Department([2006]26),Project of Science Research Project of Guangxi Education Department(200708LX180)Scientific Researc-hing fund project of Guangxi University(X081020)Fund Project for Guangxi Young People(0832008)~~
文摘[Objective] The aim was to provide scientific basis for development and utilization of Pinus elliottii Engelm resources.[Method] The extraction process of shikimic acid in Pinus elliottii Engelm was studied,and the content of shikimic acid was determined by HPLC.The HPLC conditions were as follows;Alltima NH2 （5 μm,4.6 mm × 150 mm） column separation;the mobile phase was acetonitrile-2%H3PO4 （90：10）;the flowing velocity was 1 ml/min;test wavelength was 213 nm,the width of belt was 16 nm;reference wavelength was 300 nm,the width of belt was 80 nm.[Result] By the single factor and orthogonal tests,the optimum conditions were found as follows：ethanol concentration 60%,extraction temperature 75 ℃,solid-liquid ratio 1：25,extraction time 2.5 h.The extraction rate of shikimic acid was 1.49%.[Conclusion] Shikimic acid in Pinus elliottii Engelm could be used as a new resource to develop and utilize.
文摘人类活动引起陆地生态系统氮输入水平持续升高,对全球碳循环产生影响。为探究氮素对土壤呼吸的影响,2010年6月至2012年1月,采用氮添加试验对亚热带湿地松林的土壤呼吸进行了研究。试验共设置4种氮添加水平:对照CK,0 g m^(-2)a^(-1);低氮LN,5g m^(-2)a^(-1);中氮MN,15 g m^(-2)a^(-1);高氮HN,30 g m^(-2)a^(-1);每月上、下旬采用Li-cor 8100测定土壤呼吸速率。结果表明:(1)氮添加对土壤呼吸有着显著的抑制作用,LN、MN和HN处理的土壤呼吸年累积量较CK处理分别降低了26.6%、23.7%和29.5%,而施氮处理间的土壤呼吸无显著差异;(2)林分生长期间(6-9月),施氮第1年的土壤呼吸所受抑制作用显著高于第2年同期的水平,显示施氮对土壤呼吸的影响随时间的推移而降低;(3)湿地松林土壤呼吸存在明显的季节动态,最大值出现在8月(356.32 mgCO_2 m^(-2)h^(-1)),最小值出现在1月(99.12 mg CO_2 m^(-2)h^(-1)),施氮处理并不改变土壤呼吸的季节性变化规律;(4)CK处理林分中,土壤呼吸与土壤温度间存在极显著的指数关系,而与土壤湿度无显著相关。施氮处理没有改变土壤呼吸与土壤温度之间的相互关系,但抑制了土壤呼吸的温度敏感性(Q_(10));(5)施氮处理显著减少了林分凋落物量、土壤微生物碳、氮量,并轻微抑制了细根生物量,这些改变导致了土壤呼吸的下降。上述结果表明施氮会显著抑制亚热带湿地松林的土壤呼吸速率,而这种抑制作用将随着时间的推移而降低。
基金Supported by State Forestry Administration"948"Item(2011-4-69)~~
文摘[Objective] The research aimed to study the mycorrhizal formation in the offspring tissue culture seedling bottle of Pinus elliottii with Lecanosticta acicola resistance.[Method] The mycorrhizal formation of Pinus elliottii regenerated plant in vitro was studied,and the growth condition of regenerated plant was observed.[Result] The culture substrate and the inoculation amount of mycorrhizal fungi had the big influence on the mycorrhizal formation in vitro.After the root primordia were induced by Pinus elliottii caespitose shoots,it was best to transplant in the perlite substrate and inoculate Pisolithus tinctorius,which was favorable for the mycorrhizal formation.When the two blocks of Pisolithus tinctorius were inoculated in the perlite medium,the mycorrhizal formation rate reached 84.4%.The dichotomous branching short root was the most,and 12.49 roots were formed in every main root.The mycorrhizal formation improved the survival rate of regenerated plant domestication transplantation.The mycorrhizal regenerated plant grew well in the phytotron,and the root system was developed.[Conclusion] The research provided the basis for improving the survival rate of Pinus elliottii tissue culture regenerated plant with Lecanosticta acicola resistance.
文摘The development of needle resin ducts of Pinus elliottii was studied using electron microscope. The results indicate that the resin duct development encompasses at least three distinctive development stages: the stage of initial cells, the stage of middle lamellae expansion and intercellular space appearance, and the stage of cavity enlargement and the resin duct formation. During the whole development stages, all kinds of organells in the duct cells show different ultrastructural features and regularity, among them the plastids' changing is the most striking one. On the basis of analysing the ultrastructural features of developing resin duct cells, the pattern of duct cavity formation, possible sites of resin synthesis, the manner and way of the resin elimination from the protoplast are discussed.
基金Supported by the "948" Program of State Forestry Administration(2013-4-37)~~
文摘Different ground mulching treatments were designed to observe the effects on soil moisture content in a second-generation seed orchard of Pinus elliottii. The results showed that： （1） there were extreme differences in soil moisture content Ⅰ and soil moisture content Ⅲ, but no obvious difference in soil moisture content II was observed; and （2） the high-to-low order of soil moisture content Ⅰ under different ground mulching treatments was white film〉sawdust〉black film〉the control, and the highest value was higher than the lowest one by 26.69%; the high-to-low order of soil moisture content Ⅱ under different ground mulching treatments was white film〉black film〉sawdust〉the control, and the highest value was higher than the lowest one by 20.64%; and the high-to-low order of soil moisture content Ⅲ under different ground mulching treatments was black film〉white film〉sawdust〉the control, and the highest value was higher than the lowest one by 26.61%.
