Exosomes exhibit complex biological functions and mediate a variety of biological processes,such as promoting axonal regeneration and functional recove ry after injury.Long non-coding RNAs(IncRNAs)have been reported t...Exosomes exhibit complex biological functions and mediate a variety of biological processes,such as promoting axonal regeneration and functional recove ry after injury.Long non-coding RNAs(IncRNAs)have been reported to play a crucial role in axonal regeneration.Howeve r,the role of the IncRNA-microRNAmessenger RNA(mRNA)-competitive endogenous RNA(ceRNA)network in exosome-mediated axonal regeneration remains unclear.In this study,we performed RNA transcriptome sequencing analysis to assess mRNA expression patterns in exosomes produced by cultured fibroblasts(FC-EXOs)and Schwann cells(SCEXOs).Diffe rential gene expression analysis,Gene Ontology analysis,Kyoto Encyclopedia of Genes and Genomes analysis,and protein-protein intera ction network analysis were used to explo re the functions and related pathways of RNAs isolated from FC-EXOs and SC-EXOs.We found that the ribosome-related central gene Rps5 was enriched in FC-EXOs and SC-EXOs,which suggests that it may promote axonal regeneration.In addition,using the miRWalk and Starbase prediction databases,we constructed a regulatory network of ceRNAs targeting Rps5,including 27 microRNAs and five IncRNAs.The ceRNA regulatory network,which included Ftx and Miat,revealed that exsosome-derived Rps5 inhibits scar formation and promotes axonal regeneration and functional recovery after nerve injury.Our findings suggest that exosomes derived from fibro blast and Schwann cells could be used to treat injuries of peripheral nervous system.展开更多
Recent studies have found that erythropoietin promotes the recovery of neurological function after traumatic brain injury.However,the precise mechanism of action remains unclea r.In this study,we induced moderate trau...Recent studies have found that erythropoietin promotes the recovery of neurological function after traumatic brain injury.However,the precise mechanism of action remains unclea r.In this study,we induced moderate traumatic brain injury in mice by intrape ritoneal injection of erythro poietin for 3 consecutive days.RNA sequencing detected a total of 4065 differentially expressed RNAs,including 1059 mRNAs,92 microRNAs,799 long non-coding RNAs,and 2115circular RNAs.Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses revealed that the coding and non-coding RNAs that were differentially expressed after traumatic brain injury and treatment with erythropoietin play roles in the axon guidance pathway,Wnt pathway,and MAPK pathway.Constructing competing endogenous RNA networks showed that regulatory relationship between the differentially expressed non-coding RNAs and mRNAs.Because the axon guidance pathway was repeatedly enriched,the expression of Wnt5a and Ephb6,key factors in the axonal guidance pathway,was assessed.Ephb6 expression decreased and Wnt5a expression increased after traumatic brain injury,and these effects were reversed by treatment with erythro poietin.These findings suggest that erythro poietin can promote recove ry of nerve function after traumatic brain injury through the axon guidance pathway.展开更多
目的:探讨藏医药治疗脑卒中的用药规律,为临床治疗和新药研发提供数据参考。方法:收集《藏药方剂大全》中治疗脑卒中的藏药方剂,使用Excel 2017和R version 4.1.1进行药物频次、用药剂量、药物性味、三化味、归经、功效分析,从多角度探...目的:探讨藏医药治疗脑卒中的用药规律,为临床治疗和新药研发提供数据参考。方法:收集《藏药方剂大全》中治疗脑卒中的藏药方剂,使用Excel 2017和R version 4.1.1进行药物频次、用药剂量、药物性味、三化味、归经、功效分析,从多角度探析治疗脑卒中方剂的配伍特点和用药规律。结果:药性以温性药为主,六味中以辛味为主,三化味中以苦化味为主;归经以脾经和胃经最多;药效以行气、活血、通络、清热等为主;肉豆蔻、诃子、川木香、丁香等的用药剂量均在《中华人民共和国药典》标明范围内;使用频次≥15的有肉豆蔻、诃子、川木香、红花等31味药。结论:本研究结果符合藏医临床和配伍理论,对临床治疗脑卒中的方剂和成药的开发提供数据参考。展开更多
In this work,an approach is proposed to acquire synonymous attribute phrases of named entities(NEs) from an online encyclopedia.Synonymous attribute phrases are the phrases that express the same attribute with differe...In this work,an approach is proposed to acquire synonymous attribute phrases of named entities(NEs) from an online encyclopedia.Synonymous attribute phrases are the phrases that express the same attribute with different surface forms for a class of NEs.Specifically,the proposed approach is composed of three stages.Firstly,the entries related to a given NE class are automatically selected from an online encyclopedia.Secondly,attribute phrases are extracted based on the statistics of phrase frequency.Thirdly,synonymous attributes are identified in a pairwise manner through a classification framework combining multiple features.The proposed approach is applied on Baidu Baike,a Chinese online encyclopedia,for four different NE classes.Experimental results show that the approach obtains an average precision of 74%and an average F-value of 65%for the four NE classes.In particular,thousands of synonymous attribute phrase pairs are acquired for each class,which demonstrates the effectiveness of the proposed approach.展开更多
Encyclopedia of Computational Neuroscience(ISBN:978-1-4614-6674-1,e Book ISBN:978-1-4614-6676-5)was published by Springer-Verlag New York in 2015.The editors of this book are Dieter Jaeger(Department of Biology,Emory ...Encyclopedia of Computational Neuroscience(ISBN:978-1-4614-6674-1,e Book ISBN:978-1-4614-6676-5)was published by Springer-Verlag New York in 2015.The editors of this book are Dieter Jaeger(Department of Biology,Emory University)and Ranu Jung(Department of Biomedical Engineering,Florida International University).展开更多
基金supported by the National Natural Science Foundation of China,No.81870975(to SZ)。
文摘Exosomes exhibit complex biological functions and mediate a variety of biological processes,such as promoting axonal regeneration and functional recove ry after injury.Long non-coding RNAs(IncRNAs)have been reported to play a crucial role in axonal regeneration.Howeve r,the role of the IncRNA-microRNAmessenger RNA(mRNA)-competitive endogenous RNA(ceRNA)network in exosome-mediated axonal regeneration remains unclear.In this study,we performed RNA transcriptome sequencing analysis to assess mRNA expression patterns in exosomes produced by cultured fibroblasts(FC-EXOs)and Schwann cells(SCEXOs).Diffe rential gene expression analysis,Gene Ontology analysis,Kyoto Encyclopedia of Genes and Genomes analysis,and protein-protein intera ction network analysis were used to explo re the functions and related pathways of RNAs isolated from FC-EXOs and SC-EXOs.We found that the ribosome-related central gene Rps5 was enriched in FC-EXOs and SC-EXOs,which suggests that it may promote axonal regeneration.In addition,using the miRWalk and Starbase prediction databases,we constructed a regulatory network of ceRNAs targeting Rps5,including 27 microRNAs and five IncRNAs.The ceRNA regulatory network,which included Ftx and Miat,revealed that exsosome-derived Rps5 inhibits scar formation and promotes axonal regeneration and functional recovery after nerve injury.Our findings suggest that exosomes derived from fibro blast and Schwann cells could be used to treat injuries of peripheral nervous system.
基金supported by the National Natural Science Foundation of China,No.81771355the Natural Science Foundation of Chongqing Science and Technology Bureau,Nos.CSTC2015jcyjA10096,cstc2021jcyj-msxmX0262(all to ZL)。
文摘Recent studies have found that erythropoietin promotes the recovery of neurological function after traumatic brain injury.However,the precise mechanism of action remains unclea r.In this study,we induced moderate traumatic brain injury in mice by intrape ritoneal injection of erythro poietin for 3 consecutive days.RNA sequencing detected a total of 4065 differentially expressed RNAs,including 1059 mRNAs,92 microRNAs,799 long non-coding RNAs,and 2115circular RNAs.Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses revealed that the coding and non-coding RNAs that were differentially expressed after traumatic brain injury and treatment with erythropoietin play roles in the axon guidance pathway,Wnt pathway,and MAPK pathway.Constructing competing endogenous RNA networks showed that regulatory relationship between the differentially expressed non-coding RNAs and mRNAs.Because the axon guidance pathway was repeatedly enriched,the expression of Wnt5a and Ephb6,key factors in the axonal guidance pathway,was assessed.Ephb6 expression decreased and Wnt5a expression increased after traumatic brain injury,and these effects were reversed by treatment with erythro poietin.These findings suggest that erythro poietin can promote recove ry of nerve function after traumatic brain injury through the axon guidance pathway.
文摘目的:探讨藏医药治疗脑卒中的用药规律,为临床治疗和新药研发提供数据参考。方法:收集《藏药方剂大全》中治疗脑卒中的藏药方剂,使用Excel 2017和R version 4.1.1进行药物频次、用药剂量、药物性味、三化味、归经、功效分析,从多角度探析治疗脑卒中方剂的配伍特点和用药规律。结果:药性以温性药为主,六味中以辛味为主,三化味中以苦化味为主;归经以脾经和胃经最多;药效以行气、活血、通络、清热等为主;肉豆蔻、诃子、川木香、丁香等的用药剂量均在《中华人民共和国药典》标明范围内;使用频次≥15的有肉豆蔻、诃子、川木香、红花等31味药。结论:本研究结果符合藏医临床和配伍理论,对临床治疗脑卒中的方剂和成药的开发提供数据参考。
基金Supported by the National High Technology Research and Development Programme of China(No.2008AA01Z144)the National NaturalScience Foundation of China(No.61073126,61073129)
文摘In this work,an approach is proposed to acquire synonymous attribute phrases of named entities(NEs) from an online encyclopedia.Synonymous attribute phrases are the phrases that express the same attribute with different surface forms for a class of NEs.Specifically,the proposed approach is composed of three stages.Firstly,the entries related to a given NE class are automatically selected from an online encyclopedia.Secondly,attribute phrases are extracted based on the statistics of phrase frequency.Thirdly,synonymous attributes are identified in a pairwise manner through a classification framework combining multiple features.The proposed approach is applied on Baidu Baike,a Chinese online encyclopedia,for four different NE classes.Experimental results show that the approach obtains an average precision of 74%and an average F-value of 65%for the four NE classes.In particular,thousands of synonymous attribute phrase pairs are acquired for each class,which demonstrates the effectiveness of the proposed approach.
文摘Encyclopedia of Computational Neuroscience(ISBN:978-1-4614-6674-1,e Book ISBN:978-1-4614-6676-5)was published by Springer-Verlag New York in 2015.The editors of this book are Dieter Jaeger(Department of Biology,Emory University)and Ranu Jung(Department of Biomedical Engineering,Florida International University).
