The activity of polygalacturonase inhibiting protein (PGIP)was assayed in four kind of cultures induced endo-polygalacturonase (endo-PG). It was discovered that endo-PG stimulated obviously the accumulation of PGIP. T...The activity of polygalacturonase inhibiting protein (PGIP)was assayed in four kind of cultures induced endo-polygalacturonase (endo-PG). It was discovered that endo-PG stimulated obviously the accumulation of PGIP. The PGIP contents of SuMai No. 3 and SA-Ⅱ cells,which are fungal disease-resistant,are higher than the contents of Mian Yang No. 11 and JingHua No.1. It is indicated in cell level that PGIP has relation to resistance of plant.展开更多
文摘The activity of polygalacturonase inhibiting protein (PGIP)was assayed in four kind of cultures induced endo-polygalacturonase (endo-PG). It was discovered that endo-PG stimulated obviously the accumulation of PGIP. The PGIP contents of SuMai No. 3 and SA-Ⅱ cells,which are fungal disease-resistant,are higher than the contents of Mian Yang No. 11 and JingHua No.1. It is indicated in cell level that PGIP has relation to resistance of plant.
文摘【目的】解析桃果实黏离核性状的分子机制。【方法】以不同黏离核性状的45份桃野生资源、地方品种和育成品种为试材,采用第2代测序技术鉴定2个通过连锁分析得到的、串联重复的endo-PG基因在45份种质中的SNPs(single nucleotide polymorphisms)信息;继而采用GLM(Generalized Linear Models)和MLM(Mixed Linear Models)模型进行关联分析,鉴定与黏离核性状关联的SNPs。【结果】在ppa006839m和ppa006857m这2个endo-PG基因内共鉴定到98个SNPs,平均1 SNPs/59 bp。在少数位点上,离核和黏核种质的SNPs多态性存在差异,但整体多样性水平差异不显著,暗示这2个endo-PG基因倾向于中性进化。根据98个SNPs位点信息构建的系统发育树能够反映这些种质的进化关系,即光核桃、甘肃桃最为原始,山桃其次,普通桃最为进化。GLM关联分析结果表明,在ppa006839m基因的内含子3区存在一个与黏离核性状显著关联的位点,对表型变异的解释率为21.1%,但经Bonferroni多重检验校正后,该位点并无统计学意义。而MLM模型的关联分析并没有检测到显著关联位点。【结论】研究结果虽然没能解释果实黏离核的分子机制,但暗示ppa006839m可能与该性状存在更为紧密的联系。同时,研究结果也为果实黏离核性状的分子标记辅助育种奠定基础。
