Dry-cured meat products are considerably popular around the world due to unique flavor.Proteolysis is one of the enzymatic reactions from which flavor substances are derived,which is affected by endogenous proteases.T...Dry-cured meat products are considerably popular around the world due to unique flavor.Proteolysis is one of the enzymatic reactions from which flavor substances are derived,which is affected by endogenous proteases.The purpose aimed to reveal the potential relationship between endogenous proteases and key flavor substances in dry-cured pork coppa in this paper.The dynamic changes of endogenous proteases activity,free amino acids,and volatiles during dry-cured pork coppa processing were characterized.The results showed that 5 kinds of free amino acids,Glu,Lys,Val,Ala,and Leu,were identified as significant contributors to taste.Meanwhile,key volatiles,such as hexanal,nonanal,octanal,benzaldehyde,3-methyl butanoic acid,2-methyl propanoic acid,and ethyl octanoate,greatly contributed to the flavor characteristics of dry-cured pork coppa.Further partial correlation analysis was performed to better elucidate the relationship among parameters.The results revealed that close relationship between endogenous proteases and key substances.RAP not only significantly affected the accumulation of key active-amino acids,but also affected the accumulation of ethyl octanoate,2,3-pentanedione,and 2,3-octanedione by regulating the accumulation of octanoic acid and Leu.In addition,cathepsin B and D,DPP II,DPP IV and RAP notably affected accumulation of hexanal.展开更多
Fifty dogs were inflicted with burn-blast combined injury and divided into 5 groups. All the experimental animals began to receive various amounts of fluid and sodium slat replacement 2 h after injury. Serum level of ...Fifty dogs were inflicted with burn-blast combined injury and divided into 5 groups. All the experimental animals began to receive various amounts of fluid and sodium slat replacement 2 h after injury. Serum level of endogenous digitalis-like factor (EDLF展开更多
There are increasing evidences for the presence of an endogenous digitalis-like substance (EDLS) which interacts with the digitalis receptor and acts against the digoxin serum response in human body. The plasma EDLS l...There are increasing evidences for the presence of an endogenous digitalis-like substance (EDLS) which interacts with the digitalis receptor and acts against the digoxin serum response in human body. The plasma EDLS level increases in response to fluid or salt loading. EDLS may play a role in salt and blood pressure regulation. Being considered as a volume-expanding state, pregnancy may cause an increase in maternal EDLS. However, further studies to identify the origin of EDLS in normal pregnancy are needed.展开更多
1 Results Mammalian possess receptors for digitalis,cardiac glycosides,derived from plants.As one of the results from studies on a real endogenous ligand for the receptors,so called an endogenous digitalis-like factor...1 Results Mammalian possess receptors for digitalis,cardiac glycosides,derived from plants.As one of the results from studies on a real endogenous ligand for the receptors,so called an endogenous digitalis-like factor (EDF) or substance (EDS),Inagami and Tamura isolated a promising candidate possessing a molecular weight,336.We elucidated several possible structures (Fig.1) for the factor and synthesized several modeled compounds,some of which exhibited remarkable constriction at higher concentrations t...展开更多
【目的】探究化学调控剂对大穗型水稻籽粒激素和灌浆相关miRNA表达的影响。【方法】采用大田试验,以大穗型杂交稻交源优216为试验材料,测定了喷施ABA和复配化学调控剂后水稻籽粒灌浆充实、激素含量、miRNA及靶基因表达量。【结果】ABA...【目的】探究化学调控剂对大穗型水稻籽粒激素和灌浆相关miRNA表达的影响。【方法】采用大田试验,以大穗型杂交稻交源优216为试验材料,测定了喷施ABA和复配化学调控剂后水稻籽粒灌浆充实、激素含量、miRNA及靶基因表达量。【结果】ABA和复配剂处理显著增加了水稻籽粒、尤其是弱势籽粒灌浆期间的起始灌浆势、最大及平均灌浆速率,进而增加千粒质量和提高水稻产量;ABA和复配剂处理显著增加了灌浆中前期强弱势籽粒中IAA、Z+ZR和ABA的含量,同时显著抑制强弱势籽粒花后10 d和弱势籽粒中花后10和20 d miR167和miR1432的表达量,促进相应靶基因的表达;进一步相关分析结果表明,灌浆速率和籽粒中IAA和ABA的含量呈极显著正相关关系,而与miR167和miR1432的相对表达量呈显著负相关关系。【结论】ABA和复配剂可能是抑制籽粒灌浆中前期miR167、miR1432的表达,促进其下游靶基因的表达,进而增加籽粒中IAA、Z+ZR和ABA含量,促进灌浆速率的提高和千粒质量及产量的增加。展开更多
基金financially supported by the National Natural Science Foundation of China(32001728,32172248)the Taishan Industrial Experts Program+1 种基金the Guizhou High-level Innovative Talent Training Project(Qianke Cooperation Platform Talent number[2016]5662)Guizhou Science and Technology Innovation Talent Team of Ecological Characteristic Meat Products.(QKHPTRC[2020]5004)。
文摘Dry-cured meat products are considerably popular around the world due to unique flavor.Proteolysis is one of the enzymatic reactions from which flavor substances are derived,which is affected by endogenous proteases.The purpose aimed to reveal the potential relationship between endogenous proteases and key flavor substances in dry-cured pork coppa in this paper.The dynamic changes of endogenous proteases activity,free amino acids,and volatiles during dry-cured pork coppa processing were characterized.The results showed that 5 kinds of free amino acids,Glu,Lys,Val,Ala,and Leu,were identified as significant contributors to taste.Meanwhile,key volatiles,such as hexanal,nonanal,octanal,benzaldehyde,3-methyl butanoic acid,2-methyl propanoic acid,and ethyl octanoate,greatly contributed to the flavor characteristics of dry-cured pork coppa.Further partial correlation analysis was performed to better elucidate the relationship among parameters.The results revealed that close relationship between endogenous proteases and key substances.RAP not only significantly affected the accumulation of key active-amino acids,but also affected the accumulation of ethyl octanoate,2,3-pentanedione,and 2,3-octanedione by regulating the accumulation of octanoic acid and Leu.In addition,cathepsin B and D,DPP II,DPP IV and RAP notably affected accumulation of hexanal.
文摘Fifty dogs were inflicted with burn-blast combined injury and divided into 5 groups. All the experimental animals began to receive various amounts of fluid and sodium slat replacement 2 h after injury. Serum level of endogenous digitalis-like factor (EDLF
文摘There are increasing evidences for the presence of an endogenous digitalis-like substance (EDLS) which interacts with the digitalis receptor and acts against the digoxin serum response in human body. The plasma EDLS level increases in response to fluid or salt loading. EDLS may play a role in salt and blood pressure regulation. Being considered as a volume-expanding state, pregnancy may cause an increase in maternal EDLS. However, further studies to identify the origin of EDLS in normal pregnancy are needed.
文摘1 Results Mammalian possess receptors for digitalis,cardiac glycosides,derived from plants.As one of the results from studies on a real endogenous ligand for the receptors,so called an endogenous digitalis-like factor (EDF) or substance (EDS),Inagami and Tamura isolated a promising candidate possessing a molecular weight,336.We elucidated several possible structures (Fig.1) for the factor and synthesized several modeled compounds,some of which exhibited remarkable constriction at higher concentrations t...
文摘【目的】探究化学调控剂对大穗型水稻籽粒激素和灌浆相关miRNA表达的影响。【方法】采用大田试验,以大穗型杂交稻交源优216为试验材料,测定了喷施ABA和复配化学调控剂后水稻籽粒灌浆充实、激素含量、miRNA及靶基因表达量。【结果】ABA和复配剂处理显著增加了水稻籽粒、尤其是弱势籽粒灌浆期间的起始灌浆势、最大及平均灌浆速率,进而增加千粒质量和提高水稻产量;ABA和复配剂处理显著增加了灌浆中前期强弱势籽粒中IAA、Z+ZR和ABA的含量,同时显著抑制强弱势籽粒花后10 d和弱势籽粒中花后10和20 d miR167和miR1432的表达量,促进相应靶基因的表达;进一步相关分析结果表明,灌浆速率和籽粒中IAA和ABA的含量呈极显著正相关关系,而与miR167和miR1432的相对表达量呈显著负相关关系。【结论】ABA和复配剂可能是抑制籽粒灌浆中前期miR167、miR1432的表达,促进其下游靶基因的表达,进而增加籽粒中IAA、Z+ZR和ABA含量,促进灌浆速率的提高和千粒质量及产量的增加。
