Distribution of natural killer cells and T-lymphocyte subsets in peripheral blood,gallbladder cancer and surrounding tissue

BACKGROUND: The patient with malignant tumor always show immunologic function drawback and ingravescent with tumor development, especially in the aspect of cell-mediated immunity. This study was undertaken to define the relationship between the immune function of local cells and cancer development by investigating the distribution of natural killer (NK) cells and T-lymphocyte subsets in peripheral blood, the cancer tissue and the tissue surrounding gallbladder carcinoma. METHODS: The numbers of CD4(+) and CD8(+) T-lymphocytes and NK cells were measured by flow cytometry in samples taken from gallbladder cancer tissue, the surrounding tissues and peripheral blood of 38 patients, and compared with the numbers in the peripheral blood and gallbladder tissue of 30 patients with cholecystitis as controls. RESULTS: The numbers of CD4(+) and CD8(+) T-cells and NK cells in gallbladder cancer tissues were significantly higher than those in the surrounding tissue and gallbladder with gallstone. However, the ratio of CD4(+)/CD8(+) was lower in the cancer tissue than that in the surrounding tissue and tissue from gallbladders with gallstones. The distribution of CD4(+) and CD8(+) T-cells and NK cells in mucous membrane of cholecystitis gallbladder and that in the tissue surrounding gallbladder cancer were significantly different. CONCLUSIONS: Disproportionate and imbalanced distribution of NK cells and subsets of T-lymphocytes occurs in the mucous membrane proper of gallbladder cancer and surrounding tissue. Although gallbladder cancer tissue has higher expressions of CD4(+), CD8(+) and NK cells, the immune function is low or in an inhibited state. In gallbladder cancer immunization therapy, local cellular immunological function should be enhanced and the protective barrier improved.

Effects of Intraoperative Administration of Dexmedetomidine on the Percentage of T-Lymphocyte Subsets and Natural Killer Cells in Patients with Colorectal Cancer

Study Objective: To observe the effect of dexmedetomidine (DEX) on T-lymphocyte subsets and natural killer (NK) cells in the peripheral blood of perioperative patients with colorectal cancer. Design: A random double-blind control clinical study. Setting: A university hospital. Patients: Forty patients with colorectal cancer, ASA I-П. Interventions: All patients were randomly divided into a DEX group (n = 20) and a control group (n = 20). Before induction of anesthesia, epidural catheters were placed in the L1-L2 or T12-L1 intervertebral spaces. The DEX group received 1 μg/kg of DEX (200 μg/50 ml) intravenously for 15 min prior to the surgery, which was then infused at a rate of 0.5 μg/kg/h until 30 min before the end of the surgery. The control group received an intravenous infusion of saline (50 ml) instead of DEX during the same periods as the DEX group. All patients received routine anesthesia and postoperative analgesia. Measurements: Blood samples from all patients were collected at the following time points: before anesthesia (T0), 24 h after surgery (T1), 48 h after surgery (T2) and 72 h after surgery (T3). Changes in T-lymphocyte subsets (CD3+, CD4+, CD8+, CD4+/CD8+) and NK cells were determined by flow cytometry. Main Results: Compared with the control group, the percentages of CD3+ and CD4+ cells and the CD4+/CD8+ ratio in the DEX group increased significantly from T1 to T3

Recovery of natural killer cells is mainly in post-treatment period in chronic hepatitis C patients treated with sofosbuvir plus ledipasvir

AIM To investigate how natural killer(NK) cells are affected in the elimination of hepatitis C virus(HCV) by sofosbuvir/ledipasvir, two highly effective direct-acting antivirals(DAAs). METHODS Thirteen treatment-na?ve and treatment-experienced chronic hepatitis C(CHC) patients were treated with sofosbuvir/ledipasvir, and NK cells were detected at baseline, weeks 2, 4, 8 and 12 during therapy, and week post of treatment(Pt)-12 and 24 after the end of therapy by multicolor flow cytometry and compared with those from 13 healthy controls. RESULTS All patients achieved sustained virological response. There was a significant decline in CD56^(bright) NK cell frequencies at week 8(P = 0.002) and week 12(P = 0.003), which were altered to the level comparable to healthy controls at week Pt-12, but no difference was observed in the frequency of CD56^(dim) NK cells. Compared with healthy controls, the expression levels of NKG2A, NKp30 and CD94 on NK cells from CHC patients at baseline were higher. NKG2A, NKp30 and CD94 started to recover at week 12 and reached the levels similar to those of healthy controls at week Pt-12 or Pt-24. Before treatment, patients have higher interferon(IFN)-γ and perforin levels than healthy controls, and IFN-γ started to recover at week 8 and reached the normalized level at week Pt-12. CONCLUSION NK cells of CHC patients can be affected by DAAs, and phenotypes and function of NK cells recover not at early stage but mainly after the end of sofosbuvir/ledipasvir treatment.

Impact of comorbid anxiety and depression on quality of life and cellular immunity changes in patients with digestive tract cancers

AIM:A study was performed to investigate the impact of comorbid anxiety and depression (CAD) on quality of life (QOL) and cellular immunity changes in patients with digestive tract cancers. METHODS: One hundred and fifty-six cases of both sexes with cancers of the digestive tract admitted between March 2001 and February 2004 in the Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong University were randomly enrolled in the study. Depressive and anxiety disorder diagnoses were assessed by using the Structured Clinical Interview for DSM-IV. All adult patients were evaluated with the Hamilton depressive scale (HAMD, the 24-item version), the Hamilton anxiety scale (HAMA, a modified 14-item version), quality of life questionnaire-core 30 (QLQ-C30), social support rating scale (SSRS), simple coping style questionnaire (SCSQ), and other questionnaires, respectively. In terms of HAMD ≥ 20 and HAMA ≥ 14, the patients were categorized, including CAD (n = 31) in group A, anxiety disorder (n = 23) in group B, depressive disorder (n = 37) in group C, and non-disorder (n = 65) in group D. Immunological parameters such as T-lymphocyte subsets and natural killer (NK) cell activities in peripheral blood were determined and compared among the four groups. RESULTS: The incidence of CAD was 21.15% in patients with digestive tract cancers. The average scores of social support was 43.67±7.05 for 156 cases, active coping 20.34±7.33, and passive coping 9.55±5.51. Compared with group D, subjective support was enhanced slightly in group A, but social support, objective support, and utilization of support reduced, especially utilization of support with significance (6.16 vs7.80, P<0.05); total scores of active coping decreased, while passive coping reversed; granulocytes proliferated, monocytes declined, and lymphocytes declined significantly (32.87 vs 34.00, P<0.05); moreover, the percentage of CD3, CD4, CD8 and CD56 in T lymphocyte subsets was in lower level, respectively, and CD56 showed a significant decline in group A (26.02 vs 32.20, P<0.05), however, CD4/CD8 ratio increased. Physical function, role function, fatigue, sleeplessness and constipation had significant changes among different groups by one-way ANOVA, and group A was in poor QOL. It revealed that global health-related quality of life (QL) were positively correlated with active coping and CD56; CAD was negatively correlated with QL, active coping and CD56. Furthermore, the step-wise regression analysis suggested that utilization of support, CD56, active coping, fatigue, sleeplessness and depression were significant factors contributing to QOL. CONCLUSION: CAD, which can impair QOL and cellular immunity, occurs with a higher incidence in patients with digestive tract cancers. Hence, it is essential to improve mental health for them with specifically tailored interventions.

中国健康成人外周血自然杀伤细胞及其亚群的正常值范围流式细胞学分析

目的:研究中国健康人外周血中自然杀伤(natural killer, NK)细胞及其亚群的比例和数量,初步确定其正常值范围作为临床检验的参考数值。方法:选取200例健康成年人,年龄范围为18~87岁,所有受试者被分成6个年龄组:18~30、31~40、41~50、51~60、61~70、71~87岁,收集外周血,以CD16、CD56、CD3、CD19等作为表面标记,使用流式细胞术检测NK细胞及亚群的相对比例及绝对计数。采用SPSS 27.0软件分析数据,计量资料用均数±标准差表示,同时进行t检验、方差分析或秩和检验比较各年龄组和性别组的差异,检验水准α=0.05,以P<0.05为差异有统计学意义。结果:200例健康成人受试者CD3^(-)CD56^(+)NK细胞的范围为(13.14±7.56)×10^(6)/L,CD56^(dim)CD16^(+)NK细胞的范围为(5.23±3.12)×10^(6)/L,CD56^(bright)NK细胞的范围为(85.61±7.40)×10^(6)/L,NK T细胞的范围为(4.16±3.34)×10^(6)/L,NK B细胞的范围为(0.46±0.24)×10^(6)/L。CD3^(-)CD56^(+)NK和NK T细胞在和年龄的相关性方面差异无统计学意义(P=0.417,P=0.217);随着年龄的增长,NK B和CD56^(dim)CD16~+NK细胞的数量有一定的下降趋势(r=0.234,P<0.001;r=0.099,P<0.001),尤其是在50岁以后更加明显;CD56^(bright)NK则随着年龄增长有上升趋势(r=0.143,P<0.001)。结论:NK细胞及其亚群的检测对于自身免疫病、感染性疾病和肿瘤的诊断、治疗和预后分析具有重要参考价值;本研究为临床检测NK细胞亚群提供了初步的参考范围,但尚需扩大例数,进行多中心试验以进一步明确。

中晚期非小细胞肺癌患者外周血淋巴细胞亚群特点分析

目的分析中晚期非小细胞肺癌(NSCLC)患者外周血淋巴细胞亚群的分布特点及临床意义。方法选择2022年1月至2023年4月收治的中晚期NSCLC患者93例,采用流式细胞术检测患者外周血总T、CD4^(+)T、CD8^(+)T、总B和NK细胞的比例及绝对值,分析其分布特征。结果58.06%的患者外周血淋巴细胞比例降低,35.48%的患者外周血淋巴细胞绝对值降低。流式分析显示,外周血T(84.95%)、B(76.34%)和NK(92.47%)细胞分布比例多处于正常范围,CD4^(+)/CD8^(+)比例异常占60.22%。外周血T(60.22%)、CD4^(+)T(58.06%)和CD8^(+)T细胞(64.52%)绝对值以降低为主,B细胞绝对值降低者占44.09%,NK细胞绝对值降低者占32.26%。腺癌患者外周血总T细胞比例显著低于鳞癌患者水平(P=0.047)。腺癌患者外周血NK细胞比例和绝对值均显著低于鳞癌患者水平(P值分别为0.0025、0.02)。其余指标在不同病理类型之间的分布差异无统计学意义(P>0.05)。结论中晚期NSCLC患者外周血免疫细胞减低,CD4^(+)/CD8^(+)比例失衡,NK细胞水平与NSCLC病理类型相关。

子宫内膜异位症中在位和异位内膜自然杀伤细胞的分布及数量变化

目的 :探讨子宫内膜异位症 (内异症 )患者异位、在位内膜组织中自然杀伤 (NK)细胞的数量变化及其在内异症发生、发展中可能的作用。方法 :采用CD57单克隆抗体 ,应用免疫组织化学法标记内异症患者的在位和异位子宫内膜组织中NK细胞 ,观察其分布特点并计数其密度 ,并与正常妇女子宫内膜对照研究。结果 :CD57阳性的NK细胞存在于内异症组和正常对照组的增殖期与分泌期子宫内膜组织中。内异症患者在位、异位内膜之间及与正常对照组子宫内膜中NK细胞的数量均无统计学差异(P>0.05) ,但内异症组的异位内膜NK细胞密度有增多的趋势。结论 :(1)内异症患者的子宫在位内膜和异位内膜的NK细胞的数量无明显变化 ;(2)内异症患者异位内膜中的NK细胞的功能可能受抑制 。

艾灸足三里穴与关元穴对训练小鼠免疫功能的影响

目的探讨艾灸足三里、关元穴对训练小鼠免疫功能的影响。方法将30只雄性小鼠分为安静对照组(n=10)、运动训练组(n=10)、运动+艾灸组(n=10),后两组小鼠进行6周递增负荷训练,运动+艾灸组小鼠在每次训练后1h艾灸足三里、关元两个穴位,末次训练为负重8%的力竭游泳训练。测定各组小鼠血T淋巴细胞亚群、自然杀伤细胞(NK)、自然杀伤T(NKT)细胞及脾脏、胸腺抗氧化酶的活性变化情况。结果运动训练组与安静对照组相比,CD3+、CD4+/CD8+、NK、NKT、脾脏指数(SI)、胸腺指数(TI)降低(P<0.05),脾脏、胸腺组织中谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)降低(P<0.05),丙二醛(MDA)升高(P<0.05);运动+艾灸组与安静对照组相比,CD3+、CD4+/CD8+、NK、NKT降低(P<0.05),脾脏组织中MDA升高(P<0.05),胸腺组织中SOD升高(P<0.05),CAT降低(P<0.05);运动+艾灸组与运动训练组相比,CD3+、CD4+/CD8+、NK、NKT、SI、TI升高(P<0.05),脾脏、胸腺组织MDA降低(P<0.05),GSH-PX、SOD、CAT升高(P<0.05)。结论艾灸足三里穴、关元穴可提高训练小鼠外周血CD3+、NK、NKT细胞数量,预防CD4+/CD8+比值失调;同时抑制由于训练造成的TI、SI的变化趋势,增强胸腺、脾脏组织抗氧化酶的活性。提示艾灸足三里、关元穴可改善训练引起的免疫功能下降,提高机体免疫功能。

病毒性心肌炎患者细胞免疫功能检测的临床意义

目的：探讨病毒性心肌炎患者细胞免疫功能检测的临床意义。方法：选择本院2008—01／2009—12收治的病毒性心肌炎患者75例作为实验组，以67例健康者作为对照组，分别在入院时和入院后24h内静脉采血，对治疗前后T细胞亚群、自然杀伤细胞（NK）活性及肿瘤坏死因子（TNF）进行检测和分析。结果：与入院时比较，入院24h后实验组外周血T淋巴细胞亚群CD3、CD4、CD8及CD4／CD8均有所提高，且CD3、CD4及CD4／CD8差异均具有统计学意义（P〈0．05），但仍略低于对照组，而差异不具有统计学意义；与此同时，与入院时比较，入院24h后实验组NK及TNF均有所提高，差异均具有统计学意义（P〈0．05），但仍略低于对照组，而差异不具有统计学意义。结论：更加深入地探讨病毒性心肌炎患者的免疫损伤机制将有助于更好地指导治疗，而且具有重要的临床意义。

儿童过敏性紫癜急性期免疫功能探讨

目的:总结儿童过敏性紫癜(Henoch-Schonlein purpura,HSP)的细胞及体液免疫特征,分析其与紫癜性肾炎(Henoch-Schnlein purpura nephritis,HSPN)的关系,探讨细胞免疫及体液免疫功能在HSP发病机制中的作用及临床检测价值。方法:HSP急性期患儿190例,按有无肾脏损害分为紫癜性肾炎组(HSPN)和非肾炎组(NHSPN),流式细胞仪免疫荧光法检测T淋巴细胞亚群、自然杀伤细胞(Natural killer cells,NK cells)、B淋巴细胞变化,ELISA法检测血清IL-4、IL-10、TNF-α的含量,速率散射比浊法检测外周血免疫球蛋白IgG、IgM、IgA、IgE、C3、C4水平。结果:190例HSP患儿中,合并肾脏损害33例,不合并肾脏损害157例。与对照组相比,急性期HSP组CD19细胞绝对值、IL-4、IL-10、TNF-α、IgG、IgA、IgE、补体C3显著升高(P均<0.05),CD3、CD4、CD8、NK细胞绝对值显著降低(P均<0.05);HSP患儿中,HSPN组的IL-10、IgA水平高于NHSPN组(P<0.05),两组之间其余指标的差异无统计学意义(P均>0.05)。IgA水平升高组HSPN发生率(21.64%)高于IgA水平正常组(7.14%,χ2=5.785,P<0.05)。IL-10水平与IgA呈正相关(r=0.425 9,P<0.05),IL-4水平与IgE呈正相关(r=0.541 7,P<0.05)。结论:HSP急性期存在细胞及体液免疫功能的紊乱,均参与了HSP的发病机制,表现为细胞免疫功能低下,引起炎性介质分泌增多,导致多克隆B细胞活化及免疫球蛋白的分泌增加,介导了系统性微小血管炎,其中IgA介导的体液免疫紊乱在HSP的发病机制中起了主要作用;HSPN的IgA水平升高更为显著,可能是预测HSP发生肾脏损害的一个危险因素。

长期服用茶多酚对人免疫功能和抗氧化作用的初步研究

目的 探讨长期服用茶多酚(GTP)对肝癌高危险人群免疫功能和抗氧化作用的影响。方法 以服用GTP和安慰剂满3年的1 200名肝癌高危险人群为对象,采用S-P一步染色法、MTT法和黄嘌呤氧化酶法,分别抽样检测淋巴细胞亚群、NK细胞活性和超氧化物歧化酶(SOD)。结果 服用GTP人群的CD3、CD4、CD4／CD8及NK细胞活性均显著高于服用安慰剂的对照人群(P<0.05)。CD8则明显低于对照人群(P<0.05);服药组人群血清SOD活性明显高于对照人群(P<0.05)。结论 GTP预防人类肝癌的作用机制与免疫调节功能和抗氧化作用密切相关。

原发性免疫性血小板减少症患者脾切除术前T淋巴细胞亚群检测的临床意义

目的分析激素治疗无效或停药后复发的原发性免疫性血小板减少症(ITP)患者脾切除术前T淋巴细胞亚群、自然杀伤细胞的变化,探讨其与脾切除后ITP早期复发的关系。方法收集2009年1月-2015年12月于深圳市宝安区人民医院治疗的ITP脾切除患者32例,随访时间3~60个月,按脾切除疗效分为早期复发组(脾切除后6个月内复发,PLT<30×109/L)及有效组(脾切除后PLT>30×109/L且较切除前PLT增加2倍以上)。应用流式细胞技术检测患者脾切除术前外周血T淋巴细胞亚群(CD3+CD4+、CD3+CD8+、CD3+CD4+/CD3+CD8+、CD3-CD19+)及CD56+CD16+自然杀伤细胞的百分比(CD3-/CD16+CD56+)。计量资料组间比较采用t检验,计数资料组间比较采用χ2检验或Fisher检验。结果早期复发组患者11例(占34.4%),有效组21例(占65.6%)。早期复发组脾切除术前CD3+CD4+、CD3+CD4+/CD3+CD8+明显低于有效组[(21.7±5.4)%vs(32.6±4.1)%,t=6.39,P=0.015;0.65±0.21 vs 1.29±0.36,t=3.92,P=0.003];早期复发组脾切除术前CD3+CD8+明显高于有效组[(34.5±5.9)%vs(26.8±6.6)%,t=-3.20,P=0.030];两组患者CD3-/CD16+CD56+、CD3-CD19+差异均无统计学意义(P值均>0.05)。结论行脾切除术治疗前CD3+CD4+、CD3+CD4+/CD3+CD8+低的ITP患者,术后易出现复发,提示T淋巴细胞亚群异常可作为ITP脾切除疗效预测的参考指标之一。

复方四参饮对病毒性心肌炎患者细胞免疫功能的影响

为了研究复方四参饮对病毒性心肌炎患者细胞免疫功能的影响，测定了６０例患者的自然杀伤细胞（ＮＫ细胞）活性和２０例患者的Ｔ淋巴细胞亚群。结果除了ＯＫＴ_８外都显著低于正常值（Ｐ＜０．０１）。经复方四参饮治疗后，除ＯＫＴ_８外皆有显著升高（Ｐ＜０．０５）。中医辩证分型表明，ＮＫ细胞功能下降以气阴两虚组为明显，而Ｔ细胞亚群的改变以气虚组为显著。ＮＫ细胞活性与ＯＫＴ_４／ＯＫＴ_８值呈负相关（Ｐ＜０．０５）。此外，早期ＮＫ细胞功能状况与疾病预后关系密切。

地佐辛复合舒芬太尼术后自控静脉镇痛对肿瘤患者T淋巴细胞亚群和NK细胞水平的影响

目的:观察地佐辛复合舒芬太尼术后自控静脉镇痛对肿瘤患者T淋巴细胞亚群和NK细胞水平的影响。方法:择期行胃肠道恶性肿瘤根治术患者40例,将其随机分为地佐辛复合舒芬太尼组(DS组,n=20)和舒芬太尼组(S组,n=20)。两组患者术后各时点采用视觉模拟评分(VAS评分)行镇痛效果评价;分别于麻醉前(T0)、术毕(T1)、术后12h(T2)、24h(T3)、48h(T4)抽取外周静脉血,用流式细胞仪测定其T淋巴细胞亚群(CD3+、CD4+、CD8+、CD4+/CD8+)及NK细胞水平。结果:两组患者各时点VAS评分差异无统计学意义;与T0时比较,T1～T4时两组患者CD3+、CD4+、CD4+/CD8+水平均明显降低(P<0.05),T1～T3时两组患者NK细胞水平低于麻醉前(P<0.05)。两组各时点CD8+水平变化差异无统计学意义。与S组相比,DS组在术后24 h CD3+、CD4+、CD4+/CD8+、NK细胞水平明显增高(P<0.05);术后48hDS组NK细胞水平已恢复到麻醉前水平,而S组仍处于较低水平(P<0.05)。结论:地佐辛复合舒芬太尼自控静脉镇痛更有利于肿瘤患者术后细胞免疫功能的恢复。

淫羊藿总黄酮对环磷酰胺致免疫低下小鼠的免疫调节作用

目的:研究淫羊藿总黄酮(TFE)对环磷酰胺致免疫低下小鼠的免疫调节作用。方法:Balb/C小鼠40只,雌雄各半,随机分为空白组、模型组、TFE低、中、高剂量组,每组8只。空白组腹腔注射生理盐水,其余各组连续5d腹腔注射环磷酰胺建立免疫低下模型。空白组和模型组灌胃生理盐水,TFE低、中、高剂量组分别按200、400及800mg/kg剂量灌胃TFE。给药35d后测定各组体质量、脾质量、脾细胞绝对数量、相对数量、骨髓有核细胞数量及脾脏中CD3+、CD4+、CD8+、CD19+、NK细胞数量。结果:造模结束后,模型组脾细胞绝对数量、相对数量、骨髓有核细胞数量及脾脏中CD3+、CD4+、CD8+、CD19+、NK细胞的数量低于空白组;TFE低剂量组脾脏中CD3+、CD4+、CD8+、NK细胞及骨髓有核细胞数量高于模型组;TFE中剂量组和高剂量组的脾细胞绝对数量、骨髓有核细胞数量及脾脏中CD3+、CD8+、CD19+、NK细胞数量高于模型组;而且TFE高剂量组脾细胞相对数量和CD4+也高于模型组;差异均有统计学意义(P<0.01或P<0.05)。结论:TFE可显著增强环磷酰胺所致免疫低下小鼠的免疫功能,其部分机制在于增加脾细胞绝对数量和相对数量,调节脾脏中T细胞亚群、B淋巴细胞、NK细胞数量,并促进骨髓有核细胞的增殖。

巨细胞病毒感染时免疫功能的变化及其意义

目的 探讨巨细胞病毒感染时免疫变化及其意义。方法 以 2 0名CMV IgM(Cytomegalovirus immunoglobulinM)阳性的病儿为观察对象 ,另设对照组 30例。ELISA法检测柯萨奇B组病毒抗原 (CVB Ag)、IgM抗体 (CVB IgM)、巨细胞病毒IgM抗体 (CMV IgM)、EB病毒IgM抗体 (EBV IgM) ,单克隆抗体标记间接ABC免疫法检测外周血T细胞亚群。结果 CMV感染组的LAK细胞、NK细胞活性均明显降低 (P <0 .0 1)。合并其它感染原的CMV混合感染组CD3含量减少 ,CD8含量增加 (P <0 .0 5 ) ,CD4 CD8数值明显降低 (P <0 .0 1)。病毒混合感染并合并支原体感染组CD3含量减少 (P <0 .0 5 )、CD4 CD8数值、IgA含量明显降低 (P <0 .0 1)。结论 巨细胞病毒感染影响了T细胞亚群的平衡 。

胸腺肽α1对老年晚期恶性肿瘤患者细胞免疫功能的影响

目的 :观察胸腺肽α1对老年恶性肿瘤患者细胞免疫功能的影响。方法 :30例老年恶性肿瘤患者 ,用胸腺肽α1治疗 2个月 ,第 1个月每日 1.6 mg,第 2个月隔日 1.6 mg,均为皮下注射 ,观察治疗前后 T细胞亚群及 NK细胞、患者生活质量、血常规及肝肾功能变化。结果 :30例患者用胸腺肽α1前 ,CD4、CD4 /CD8、NK细胞分别为 (32 .33± 6 .2 5 ) %、(0 .77± 0 .2 3) %、(16 .0 5± 6 .79) % ,治疗后为 (39.4 2± 9.2 6 ) %、(1.19± 0 .5 3) %、(2 4 .37± 8.2 3) %。治疗前后比较均有显著意义 (P<0 .0 5 ) ,患者生活质量分值治疗后比治疗前显著提高 (P<0 .0 5 )。结论 :胸腺肽α1能提高老年晚期恶性肿瘤患者的细胞免疫功能 ,是较理想的免疫增强剂。

大肠癌患者外周血T细胞亚群和NK细胞活性检测的临床意义

目的 探讨大肠癌患者外周血T淋巴细胞亚群及NK细胞活性检测的临床意义。方法 采用SAP法及LDH释放法 ,对 5 7例大肠癌患者和 33例正常人的外周血T细胞亚群及NK细胞活性进行检测。结果 大肠癌患者CD+ 4细胞值、CD+ 4/CD+ 8比值下降且NK细胞活性显著降低 ,CD+ 8细胞值显著高于对照组 ;这种变化随临床分期期别的增高更加显著 (P <0 .0 5 ) ;化疗后CD+ 4细胞值、CD+ 4/CD+ 8比值的下降和NK细胞活性的降低及CD+ 8细胞值的增高更为突出 (P <0 .0 1) ;术后发生转移的患者 ,CD+ 4细胞值、CD+ 4/CD+ 8比值下降及NK细胞活性下降 ,CD+ 8细胞值再回升。结论 大肠癌患者细胞免疫功能明显紊乱 ,并随病情进展及肿瘤转移而加重。切除肿瘤有助于改善患者细胞免疫功能 。

氟比洛芬酯联合吗啡镇痛对胃癌患者T淋巴细胞亚群及自然杀伤细胞的影响

目的观察氟比洛芬酯联合吗啡镇痛对胃癌根治术患者围术期外周血T淋巴细胞亚群及自然杀伤(NK)细胞的影响。方法40例择期全麻下行胃癌根治术患者随机分为氟比洛芬酯组(A组)和吗啡组(B组),每组20例,分别于术前0.5h静注氟比洛芬酯或安慰药英脱利匹特,术后距第一次给药6h再次静注氟比洛芬酯或英脱利匹特。两组患者术后均行患者自控静脉镇痛(PCIA)。于麻醉前、手术开始后2h、术后24、48、120h五个时点用流式细胞仪检测T淋巴细胞亚群(CD3+、CD4+、CD8+)及NK细胞(CD3-CD16+CD56+)。结果与麻醉前比较,两组CD3+、CD4+、CD4+/CD8+和NK细胞在手术2h、术后24、48h均明显降低(P<0.05);术后120hCD3-CD16+CD56+仍未恢复至麻醉前水平(P<0.05)。与B组比较,A组CD3+、CD4+、CD4+/CD8+在术后24h下降幅度较小(P<0.05),而NK细胞则在手术2h和术后24h下降幅度较小(P<0.05)。结论胃癌根治术患者围术期用氟比洛芬酯联合吗啡镇痛较单用吗啡镇痛对T淋巴细胞亚群和NK细胞有保护作用。

消化系统肿瘤患者外周血T淋巴细胞亚群及NK细胞分析与临床意义

目的通过观察外周血T淋巴细胞亚群及NK细胞活性在消化系统肿瘤患者中的表达,探讨消化系统肿瘤患者的细胞免疫功能状况及临床意义。方法采用流式细胞分析法对123例消化系统肿瘤患者外周血T淋巴细胞亚群及NK细胞进行检测,并与35例健康对照组比较。结果与健康对照组比较,消化系统各肿瘤组患者外周血总T细胞(CD3+)、T4细胞(CD3+CD4+)、NK细胞(CD3-CD16/56+)百分率下降(P<0.01);T4/T8(CD3+CD4+/CD3+CD8+)比值降低(P<0.05);T8细胞(CD3+CD8+)百分率明显升高(P<0.05);消化系统各肿瘤组间差异无统计学意义(P>0.05)。结论消化系统肿瘤患者外周血T淋巴细胞亚群及NK细胞活性表达较健康对照组显著降低,表明消化系统肿瘤患者细胞免疫功能低下。检测消化系统肿瘤患者外周血T淋巴细胞亚群和NK细胞水平对评估患者的细胞免疫功能、监测抗瘤疗效和判断预后均具有一定的临床意义。