Although upconversion nanoparticle(UCNP)-based luminescence resonance energy transfer(LRET)has attracted intensive attention,its application in sensing strategies for biomacromolecule detection is still limited due to...Although upconversion nanoparticle(UCNP)-based luminescence resonance energy transfer(LRET)has attracted intensive attention,its application in sensing strategies for biomacromolecule detection is still limited due to the low energy-transfer efficiency.Here,we develop an energy pumping strategy to enhance the upconversion LRET efficiency of UCNP from 3.6% to 70.3%.Surface collector dye cyanine-3(Cy3)is modified on the UCNP surface to concentrate the scattered emission energy from interior UCNP to particle surface and then further pumped to the surface extended acceptor.Using caspase-3 as a target,its recognition and cleavage of QSY7-labeled peptide co-assembled on UCNP-Cy3 leads to the release of QSY7 to recover Cy3 fluorescence.Owing to the multiple emissions of UCNP,this system can be integrated with another surface collector to enhance reactive oxygen species(ROS)generation for photodynamic therapy(PDT).Caspase-3,as a PDT product,is imaged in situ with an internal standard to self-evaluate the PDT effect.展开更多
基金the National Natural Science Foundation of China(nos.21635005,21974064,22022405,21827812,21890741,22004062,and 22004068)Natural Science Foundation of Jiangsu Province for distinguished Young Scholars(no.BK20200010)+2 种基金Specially appointed Professor Foundation of Jiangsu ProvinceChina Postdoctoral Science Foundation(no.252986)Program for Innovative Talents and Entrepreneurs of Jiangsu Province.
文摘Although upconversion nanoparticle(UCNP)-based luminescence resonance energy transfer(LRET)has attracted intensive attention,its application in sensing strategies for biomacromolecule detection is still limited due to the low energy-transfer efficiency.Here,we develop an energy pumping strategy to enhance the upconversion LRET efficiency of UCNP from 3.6% to 70.3%.Surface collector dye cyanine-3(Cy3)is modified on the UCNP surface to concentrate the scattered emission energy from interior UCNP to particle surface and then further pumped to the surface extended acceptor.Using caspase-3 as a target,its recognition and cleavage of QSY7-labeled peptide co-assembled on UCNP-Cy3 leads to the release of QSY7 to recover Cy3 fluorescence.Owing to the multiple emissions of UCNP,this system can be integrated with another surface collector to enhance reactive oxygen species(ROS)generation for photodynamic therapy(PDT).Caspase-3,as a PDT product,is imaged in situ with an internal standard to self-evaluate the PDT effect.
