In connection with the current prospect of decarbonization of coal energy through the use of small nuclear power plants (SNPPs) at existing TPPs as heat sources for heat supply to municipal heating networks, there is ...In connection with the current prospect of decarbonization of coal energy through the use of small nuclear power plants (SNPPs) at existing TPPs as heat sources for heat supply to municipal heating networks, there is a technological need to improve heat supply schemes to increase their environmental friendliness and efficiency. The paper proves the feasibility of using the heat-feeding mode of ASHPs for urban heat supply by heating the network water with steam taken from the turbine. The ratio of electric and thermal power of a “nuclear” combined heat and power plant is given. The advantage of using a heat pump, which provides twice as much electrical power with the same heat output, is established. Taking into account that heat in these modes is supplied with different potential, the energy efficiency was used to compare these options. To increase the heat supply capacity, a scheme with the use of a high-pressure heater in the backpressure mode and with the heating of network water with hot steam was proposed. Heat supply from ASHPs is efficient and environmentally friendly even in the case of significant remoteness of heat consumers.展开更多
Concentrating Solar Power(CSP)plants offer a promising way to generate low-emission energy.However,these plants face challenges such as reduced sunlight during winter and cloudy days,despite being located in high sola...Concentrating Solar Power(CSP)plants offer a promising way to generate low-emission energy.However,these plants face challenges such as reduced sunlight during winter and cloudy days,despite being located in high solar radiation areas.Furthermore,their dispatch capacities and yields can be affected by high electricity consumption,particularly at night.The present work aims to develop an off-design model that evaluates the hourly and annual performances of a parabolic trough power plant(PTPP)equipped with a waste heat recovery system.The study aims to compare the performances of this new layout with those of the conventional Andasol 1 plant,with the aim of assessing the improvements achieved in the new design.Based on the results,it can be concluded that the new layout has increased the annual generated power to almost 183 GWh(an increase of about 7.60% is achieved compared to the Andasol 1 layout that generates 169 GWh annually).Additionally,the proposed installation has achieved an efficiency of 20.55%,which represents a 7.87% increase compared to the previous design(19.05%).The Levelized Cost of Electricity(LCOE)of the new layout has been reduced by more than 5.8% compared to the Andasol 1 plant.Specifically,it has decreased from 13.11 to 12.35 c/kWh.This reduction in LCOE highlights the improved cost-effectiveness of the newlayout,making it amore economically viable option for generating electricity compared to the conventional Andasol 1 plant.展开更多
When designing a solar power plant, it is much more important to avoid the shadow on the PV Panels. As the shadow falls on the PV Panels;it significantly reduces the generation of required power as planned and designe...When designing a solar power plant, it is much more important to avoid the shadow on the PV Panels. As the shadow falls on the PV Panels;it significantly reduces the generation of required power as planned and designed. This research paper and case study will help a lot to avoid shadow, especially when selecting inter-row spacing between the strings of solar power plants.展开更多
The evaporator is the main part of a quick-freeze equipment. There are many factors influencing the heat transfer coefficient of an evaporator. The most important factors among them are the fin shape, tube diameter, d...The evaporator is the main part of a quick-freeze equipment. There are many factors influencing the heat transfer coefficient of an evaporator. The most important factors among them are the fin shape, tube diameter, distance of fin space, frost, and velocity of air flow etc. They mainly influence the thermal efficiency of an evaporator, and therefore its thermal efficiency has direct relationship with the whole efficiency of the quick freeze plant. Evaporators with different structural types have different heat transfer efficiency, in order to obtain high efficiency structure of evaporator, 8 evaporator models with different fin shape, tube diameter and tube arrangement are analyzed and compared. The calculation results show that the integral waved fins, equilateral-triangle arranged small diameter tubes and varying fin-spacing has the highest heat transfer coefficient. The experimental result also shows that the evaporator with this type of structure has better thermal efficiency. The experimental result is in good agreement with the calculation result, it can instruct engineering design for usual designer. A real quick-freeze equipment is designed and put into production. The result shows that, compared with traditional domestic quick-freeze equipments, this equipment decreases by 40% in size and by 20% in energy consumption.展开更多
An experiment was conducted to investigate the response of salicylic acid as a second messenger to the heat stress in grape plants. For this purpose, all leaves of grape (Vitis vinifera×V. labrussa L. cv. Jingxiu...An experiment was conducted to investigate the response of salicylic acid as a second messenger to the heat stress in grape plants. For this purpose, all leaves of grape (Vitis vinifera×V. labrussa L. cv. Jingxiu) plants were removed except the 3rd, 4th, 5th, 6th, and 7th ones. The 5th leaf was fed with C-SA, and the 4th and 6th leaves were exposed to high 14 temperature at 40±0.5°C. It was observed that more C-SA transported out from the 5th leaf and the distribution of C-SA 14 14 in each organ of plant altered in response to heat stress. The accumulation of C-SA in both the 4th and 6th leaves being 14 exposed to high temperature was at least three times higher than that in control. The distribution of C-SA in other distal 14 leaves (the 3rd and 7th leaf) decreased, but more C-SA accumulated in stems adjacent to the 4th or 6th leaf exposed to 14 high temperature. In addition, there was more C-SA being transported upwards or downwards while the 4th and 6th 14 leaves were exposed to high temperature respectively. Therefore, our results suggested that SA was closely involved in signal transduction of heat stress in grape plants. However, the ratio of C radioactivity assayed after SA being extracted 14 to that of direct assay with apparatus was more than 70%, which indicated about 30% C was lost or catabolized during 14 transportation.展开更多
The performance of a 270 MW (9 × 30 MW) AES Corporation barge mounted gas turbine power plant in Nigeria is evaluated using the heat rate and entropy generation by the components of the plant to characterize the ...The performance of a 270 MW (9 × 30 MW) AES Corporation barge mounted gas turbine power plant in Nigeria is evaluated using the heat rate and entropy generation by the components of the plant to characterize the irreversibility in each component when operating at different loads between 90% and 25%. The power plants have the peculiarity that three of the plants were supplied by three (3) different Original Equipment Manufacturers (OEM);A, B and C. This study is sequel to the fact that the gas turbines were the first independent power plants in the country and after more than fifteen years of operation, it is reasonable to evaluate the performance of the major components. By analyzing the thermodynamic performance of these components, the study demonstrates the utility value of exergy efficiency as an important parameter in the evaluation of major components in a gas power plant. Exergy efficiency is shown to be an important parameter in ranking the power plant components, identifying and quantifying the possible areas of reduction in thermodynamic losses and improvement in efficiencies. A new relationship is derived to demonstrate the correlation between the exergy efficiency and the heat rate of a 30 MW gas power plant. The prediction of the derived relationship correlates well with the observed operational performance of the 30 MW power plants. The combustion chamber in each of the plants provides the maximum exergy destruction during operation. Its exergy efficiency is shown to exhibit good correlation with its energy efficiency and the plant rational exergy. The implication is that from an operational and component selection viewpoint in the specifications of a gas power plant, knowledge of the Heat Rate which is usually provided by the OEM is adequate to make a reasonable inference on the performance of some critical components of the plant.展开更多
Producing enough tomato to meet market demand sustainably has not been feasible in the tropics like Ghana. Attempts to improve production using gre</span><span style="font-family:Verdana;">enhous...Producing enough tomato to meet market demand sustainably has not been feasible in the tropics like Ghana. Attempts to improve production using gre</span><span style="font-family:Verdana;">enhouse facilities have not addressed the challenge because of high-</span><span style="font-family:Verdana;">temperature conditions in the greenhouse, which are difficult to manage. Heat stress, arising from high temperatures, hinder the performance of tomato in terms of fruit set and yield. Moreover, the impending climate change is expected to impose more unfavorable environmental conditions on crop production</span><span style="font-family:Verdana;">. An experiment was conducted in (greenhouse at Chiba Un</span><span style="font-family:Verdana;">iversity, Japan) summer period, which has similar high-temperature conditions like Ghana. This work sought to increase the yield of a hea</span><span style="font-family:Verdana;">t-tolerant tomato using a state-of-the-art hydroponic system thr</span><span style="font-family:Verdana;">ough high-density planting. The outcome of this work was intended for adoption and practice in Ghana. A Heat-tolerant tomato “Nkansah HT” along with Lebombo and Jaguar cultivars, were grown at high and low plant densities (4.1 and 2.7 plants m</span><sup><span style="font-family:Verdana;">-2</span></sup><span style="font-family:Verdana;"> respectively).</span></span><a name="_Hlk72355905"></a><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">Each plant was grown in a low substrate volume culture (0.5 L plant</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) </span><a name="_Hlk72267699"></a><span style="font-family:Verdana;">in a recirculating nutrient film technique (NFT) hydroponic system</span></span><span style="font-family:""><span style="font-family:Verdana;">. Parameters measured were plant growth and dry matter assimilation at 12 week</span><span style="font-family:Verdana;">s after transplanting, and the generative components. Results sh</span><span style="font-family:Verdana;">owed that a high plant density increased plant height but reduced chlorophyll content by</span><span style="font-family:Verdana;"> 9.6%. </span></span><span style="font-family:Verdana;">Under temperature stress conditions, the three cultivars reco</span><span style="font-family:Verdana;">rded more than 95% fruit set, but plant density did not affect the fruit set and the incidence of blossom end rot (BER).</span><span style="font-family:Verdana;"> The incidence of BER reduced the marketable yield of the Jaguar cultivar by 51% but, this physiological disorder was not recorded in the HT and the Lebombo cultivars. A high-density planting increased the yield per unit area increased by 38.9%. However, it is uneconomical to cultivate the Jaguar cultivar under a heat stress condition due to its high susceptibility to blossom end rot. To improve the yield of tomatoes under tropical heat stress with a threatening climate change condition, the HT is a better cultivar suited for high-density planting. This study shows that high-density cultivation of the HT cultivar in NFT hydroponic system has the potential to increase Ghana’s current tomato yield by 4.8 times.展开更多
[ Objective ] Heat shock factors (HSFs) are the major transcription factors of eukaryotic heat shock responses. This study aims to investigate the adversity stress tolerance functions of Arabidopsis heat shock facto...[ Objective ] Heat shock factors (HSFs) are the major transcription factors of eukaryotic heat shock responses. This study aims to investigate the adversity stress tolerance functions of Arabidopsis heat shock factor AtHsfAla, which has important significance for in-depth understanding of adversity stress tolerance mechanisms of plants and further utilization of heat shock factor genes. [Method] Genomic DNA of Arabidopsis was extracted with CTAB method and purified to obtain Arabidopsis DNA samples for in vitro site-specific recombination cloning ( Gateway cloning) to construct plant expression vector of heat shock factor AtHs- fAla. Firstly, donor vector pDONR 201/AtHsfAla was constructed based on attB and attP site-specific recombination method (BP reaction), to identify E. coli transformants harboring correct sequence of AtHsfAla by sequencing; secondly, plant expression vector pBTWG2/AttlsfAla overexpressing Arabidopsis heat shock factor AtHsfAla was constructed based on attL and attR site-specific recombination method (LR reaction), to screen E. coli transformants harboring target plasmid. [ Result] Plant expression vector of Arabidopsis heat shock factor gene AtHsfAla was constructed successfully. [ Conclusion] This study not only provided experimental materials for acquiring transgenic plants overexpressing heat shock transcription factor AtHsfAla, but also laid the foundation for further investigation of the diversity of adversity stress tolerance functions reanlated by HSFs.展开更多
Objective: To construct plant transformation vector containing Escherichia coli heat-labile enterotoxin B subunit(LT-B) gene and generate LT-B transgenic tobacco plants. Methods: The LT-B coding sequence was amplified...Objective: To construct plant transformation vector containing Escherichia coli heat-labile enterotoxin B subunit(LT-B) gene and generate LT-B transgenic tobacco plants. Methods: The LT-B coding sequence was amplified from pMMB68 by PCR, subcloned into middle vector pUCmT and binary vector pBI121 to obtain plant expression vector pBI-LTB, in which LT-B expression was controlled under the Cauliflower mosaic virus (CaMV) 35S promoter. The tobacco plants (Nicotiana tobacum L.Cuttivar Xanthi) were transformed by co-cultivating leaf discs method via Agrobacterium tumefaciens LBA4404 harboring the plant expression vector. The regenerated transgenic tobacco plants were selected by kanamycin and confirmed by PCR, Southern blot, Western blot and ELISA. Results:LT-B gene integrated in the tobacco genomic DNA and were expressed in 9 strains of transgenic tobacco plants. The yield was varied from 3.36-10.56 ng/mg total soluble tobacco leaf protein. Conclusion: The plant binary expression vector pBI-LTB was constructed successfully, and transgenic LT-B tobacco plants was generated, and confirmed by Southern blot. The protein LT-B expressed by engineered plants was identified by Western blot analysis and had the expected molecular weight of LT-B pentamer protein. This result is an important step close to developing an edible vaccine and supplying a mucasal immunoajuvant, which will contribute to the prevention of mucosa-route evading pathogen.展开更多
文摘In connection with the current prospect of decarbonization of coal energy through the use of small nuclear power plants (SNPPs) at existing TPPs as heat sources for heat supply to municipal heating networks, there is a technological need to improve heat supply schemes to increase their environmental friendliness and efficiency. The paper proves the feasibility of using the heat-feeding mode of ASHPs for urban heat supply by heating the network water with steam taken from the turbine. The ratio of electric and thermal power of a “nuclear” combined heat and power plant is given. The advantage of using a heat pump, which provides twice as much electrical power with the same heat output, is established. Taking into account that heat in these modes is supplied with different potential, the energy efficiency was used to compare these options. To increase the heat supply capacity, a scheme with the use of a high-pressure heater in the backpressure mode and with the heating of network water with hot steam was proposed. Heat supply from ASHPs is efficient and environmentally friendly even in the case of significant remoteness of heat consumers.
文摘Concentrating Solar Power(CSP)plants offer a promising way to generate low-emission energy.However,these plants face challenges such as reduced sunlight during winter and cloudy days,despite being located in high solar radiation areas.Furthermore,their dispatch capacities and yields can be affected by high electricity consumption,particularly at night.The present work aims to develop an off-design model that evaluates the hourly and annual performances of a parabolic trough power plant(PTPP)equipped with a waste heat recovery system.The study aims to compare the performances of this new layout with those of the conventional Andasol 1 plant,with the aim of assessing the improvements achieved in the new design.Based on the results,it can be concluded that the new layout has increased the annual generated power to almost 183 GWh(an increase of about 7.60% is achieved compared to the Andasol 1 layout that generates 169 GWh annually).Additionally,the proposed installation has achieved an efficiency of 20.55%,which represents a 7.87% increase compared to the previous design(19.05%).The Levelized Cost of Electricity(LCOE)of the new layout has been reduced by more than 5.8% compared to the Andasol 1 plant.Specifically,it has decreased from 13.11 to 12.35 c/kWh.This reduction in LCOE highlights the improved cost-effectiveness of the newlayout,making it amore economically viable option for generating electricity compared to the conventional Andasol 1 plant.
文摘When designing a solar power plant, it is much more important to avoid the shadow on the PV Panels. As the shadow falls on the PV Panels;it significantly reduces the generation of required power as planned and designed. This research paper and case study will help a lot to avoid shadow, especially when selecting inter-row spacing between the strings of solar power plants.
基金This project is supported by National Key Technologies R&D Programme,China (No.2001BA501A22).
文摘The evaporator is the main part of a quick-freeze equipment. There are many factors influencing the heat transfer coefficient of an evaporator. The most important factors among them are the fin shape, tube diameter, distance of fin space, frost, and velocity of air flow etc. They mainly influence the thermal efficiency of an evaporator, and therefore its thermal efficiency has direct relationship with the whole efficiency of the quick freeze plant. Evaporators with different structural types have different heat transfer efficiency, in order to obtain high efficiency structure of evaporator, 8 evaporator models with different fin shape, tube diameter and tube arrangement are analyzed and compared. The calculation results show that the integral waved fins, equilateral-triangle arranged small diameter tubes and varying fin-spacing has the highest heat transfer coefficient. The experimental result also shows that the evaporator with this type of structure has better thermal efficiency. The experimental result is in good agreement with the calculation result, it can instruct engineering design for usual designer. A real quick-freeze equipment is designed and put into production. The result shows that, compared with traditional domestic quick-freeze equipments, this equipment decreases by 40% in size and by 20% in energy consumption.
基金the National Natural Science Foundation of China(30070531,30270918).
文摘An experiment was conducted to investigate the response of salicylic acid as a second messenger to the heat stress in grape plants. For this purpose, all leaves of grape (Vitis vinifera×V. labrussa L. cv. Jingxiu) plants were removed except the 3rd, 4th, 5th, 6th, and 7th ones. The 5th leaf was fed with C-SA, and the 4th and 6th leaves were exposed to high 14 temperature at 40±0.5°C. It was observed that more C-SA transported out from the 5th leaf and the distribution of C-SA 14 14 in each organ of plant altered in response to heat stress. The accumulation of C-SA in both the 4th and 6th leaves being 14 exposed to high temperature was at least three times higher than that in control. The distribution of C-SA in other distal 14 leaves (the 3rd and 7th leaf) decreased, but more C-SA accumulated in stems adjacent to the 4th or 6th leaf exposed to 14 high temperature. In addition, there was more C-SA being transported upwards or downwards while the 4th and 6th 14 leaves were exposed to high temperature respectively. Therefore, our results suggested that SA was closely involved in signal transduction of heat stress in grape plants. However, the ratio of C radioactivity assayed after SA being extracted 14 to that of direct assay with apparatus was more than 70%, which indicated about 30% C was lost or catabolized during 14 transportation.
文摘The performance of a 270 MW (9 × 30 MW) AES Corporation barge mounted gas turbine power plant in Nigeria is evaluated using the heat rate and entropy generation by the components of the plant to characterize the irreversibility in each component when operating at different loads between 90% and 25%. The power plants have the peculiarity that three of the plants were supplied by three (3) different Original Equipment Manufacturers (OEM);A, B and C. This study is sequel to the fact that the gas turbines were the first independent power plants in the country and after more than fifteen years of operation, it is reasonable to evaluate the performance of the major components. By analyzing the thermodynamic performance of these components, the study demonstrates the utility value of exergy efficiency as an important parameter in the evaluation of major components in a gas power plant. Exergy efficiency is shown to be an important parameter in ranking the power plant components, identifying and quantifying the possible areas of reduction in thermodynamic losses and improvement in efficiencies. A new relationship is derived to demonstrate the correlation between the exergy efficiency and the heat rate of a 30 MW gas power plant. The prediction of the derived relationship correlates well with the observed operational performance of the 30 MW power plants. The combustion chamber in each of the plants provides the maximum exergy destruction during operation. Its exergy efficiency is shown to exhibit good correlation with its energy efficiency and the plant rational exergy. The implication is that from an operational and component selection viewpoint in the specifications of a gas power plant, knowledge of the Heat Rate which is usually provided by the OEM is adequate to make a reasonable inference on the performance of some critical components of the plant.
文摘Producing enough tomato to meet market demand sustainably has not been feasible in the tropics like Ghana. Attempts to improve production using gre</span><span style="font-family:Verdana;">enhouse facilities have not addressed the challenge because of high-</span><span style="font-family:Verdana;">temperature conditions in the greenhouse, which are difficult to manage. Heat stress, arising from high temperatures, hinder the performance of tomato in terms of fruit set and yield. Moreover, the impending climate change is expected to impose more unfavorable environmental conditions on crop production</span><span style="font-family:Verdana;">. An experiment was conducted in (greenhouse at Chiba Un</span><span style="font-family:Verdana;">iversity, Japan) summer period, which has similar high-temperature conditions like Ghana. This work sought to increase the yield of a hea</span><span style="font-family:Verdana;">t-tolerant tomato using a state-of-the-art hydroponic system thr</span><span style="font-family:Verdana;">ough high-density planting. The outcome of this work was intended for adoption and practice in Ghana. A Heat-tolerant tomato “Nkansah HT” along with Lebombo and Jaguar cultivars, were grown at high and low plant densities (4.1 and 2.7 plants m</span><sup><span style="font-family:Verdana;">-2</span></sup><span style="font-family:Verdana;"> respectively).</span></span><a name="_Hlk72355905"></a><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">Each plant was grown in a low substrate volume culture (0.5 L plant</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) </span><a name="_Hlk72267699"></a><span style="font-family:Verdana;">in a recirculating nutrient film technique (NFT) hydroponic system</span></span><span style="font-family:""><span style="font-family:Verdana;">. Parameters measured were plant growth and dry matter assimilation at 12 week</span><span style="font-family:Verdana;">s after transplanting, and the generative components. Results sh</span><span style="font-family:Verdana;">owed that a high plant density increased plant height but reduced chlorophyll content by</span><span style="font-family:Verdana;"> 9.6%. </span></span><span style="font-family:Verdana;">Under temperature stress conditions, the three cultivars reco</span><span style="font-family:Verdana;">rded more than 95% fruit set, but plant density did not affect the fruit set and the incidence of blossom end rot (BER).</span><span style="font-family:Verdana;"> The incidence of BER reduced the marketable yield of the Jaguar cultivar by 51% but, this physiological disorder was not recorded in the HT and the Lebombo cultivars. A high-density planting increased the yield per unit area increased by 38.9%. However, it is uneconomical to cultivate the Jaguar cultivar under a heat stress condition due to its high susceptibility to blossom end rot. To improve the yield of tomatoes under tropical heat stress with a threatening climate change condition, the HT is a better cultivar suited for high-density planting. This study shows that high-density cultivation of the HT cultivar in NFT hydroponic system has the potential to increase Ghana’s current tomato yield by 4.8 times.
基金Supported by National Natural Science Foundation of China(31060039,31260061)Natural Science Foundation of Yunnan Province(2010ZC163)+1 种基金Project of Kunming University(YJL11025)Fund for Key Discipline Construction of Kunming University
文摘[ Objective ] Heat shock factors (HSFs) are the major transcription factors of eukaryotic heat shock responses. This study aims to investigate the adversity stress tolerance functions of Arabidopsis heat shock factor AtHsfAla, which has important significance for in-depth understanding of adversity stress tolerance mechanisms of plants and further utilization of heat shock factor genes. [Method] Genomic DNA of Arabidopsis was extracted with CTAB method and purified to obtain Arabidopsis DNA samples for in vitro site-specific recombination cloning ( Gateway cloning) to construct plant expression vector of heat shock factor AtHs- fAla. Firstly, donor vector pDONR 201/AtHsfAla was constructed based on attB and attP site-specific recombination method (BP reaction), to identify E. coli transformants harboring correct sequence of AtHsfAla by sequencing; secondly, plant expression vector pBTWG2/AttlsfAla overexpressing Arabidopsis heat shock factor AtHsfAla was constructed based on attL and attR site-specific recombination method (LR reaction), to screen E. coli transformants harboring target plasmid. [ Result] Plant expression vector of Arabidopsis heat shock factor gene AtHsfAla was constructed successfully. [ Conclusion] This study not only provided experimental materials for acquiring transgenic plants overexpressing heat shock transcription factor AtHsfAla, but also laid the foundation for further investigation of the diversity of adversity stress tolerance functions reanlated by HSFs.
基金Supported by the National Natural Science Foundation ofChina (No. 30070848)
文摘Objective: To construct plant transformation vector containing Escherichia coli heat-labile enterotoxin B subunit(LT-B) gene and generate LT-B transgenic tobacco plants. Methods: The LT-B coding sequence was amplified from pMMB68 by PCR, subcloned into middle vector pUCmT and binary vector pBI121 to obtain plant expression vector pBI-LTB, in which LT-B expression was controlled under the Cauliflower mosaic virus (CaMV) 35S promoter. The tobacco plants (Nicotiana tobacum L.Cuttivar Xanthi) were transformed by co-cultivating leaf discs method via Agrobacterium tumefaciens LBA4404 harboring the plant expression vector. The regenerated transgenic tobacco plants were selected by kanamycin and confirmed by PCR, Southern blot, Western blot and ELISA. Results:LT-B gene integrated in the tobacco genomic DNA and were expressed in 9 strains of transgenic tobacco plants. The yield was varied from 3.36-10.56 ng/mg total soluble tobacco leaf protein. Conclusion: The plant binary expression vector pBI-LTB was constructed successfully, and transgenic LT-B tobacco plants was generated, and confirmed by Southern blot. The protein LT-B expressed by engineered plants was identified by Western blot analysis and had the expected molecular weight of LT-B pentamer protein. This result is an important step close to developing an edible vaccine and supplying a mucasal immunoajuvant, which will contribute to the prevention of mucosa-route evading pathogen.