In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, rates of primary production and physiological status. This study describes a preliminary investigation on...In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, rates of primary production and physiological status. This study describes a preliminary investigation on the joint application of the three kinds of fluorescence analysis in the physiological study of microalgae. Flow cytometry and fluorescence spectrometry were used to obtain the in vivo static fluorescence information of pigments, and a Pulsed-Amplitude-Modulation chlorophyll fluorometer was used to detect the dynamic fluorescence of chlorophyll. The validity of the joint application was proved by analyzing two labora- tory cultured Arctic microalgae, Pseudo-nitzschia delicatissima (Bacillariophyceae) and Thalassiosira sp. The higher value of minimum fluorescence yield in dark-adapted state (Fo), actual photochemical efficiency of PSll (ФPSII), and electron transport rate (ETR) exhibited positive results in a higher cell abundance and chlorophyll a content of P. delicatissima; whereas higher fl-carotene content of Thalassiosira sp. played an important role in the protection of photosynthesis.展开更多
A three dimensional model which considers the effects of turbulence and detailed chemi cal kinetics is built to simulate the combustion process of engine fueled by compressed nature gas (CNG). The model is accompli...A three dimensional model which considers the effects of turbulence and detailed chemi cal kinetics is built to simulate the combustion process of engine fueled by compressed nature gas (CNG). The model is accomplished by integrating CFD software KIVA3V and chemical kinetic soft- ware CHEMKINII. Meanwhile, a turbulence combustion model which is suitable for describing the reaction rate under the coupled simulation is developed to balance the effects of turbulence and de tailed chemical kinetics. To reduce the computation time, subsequent development of the simulation code is realized, which enables the simulation code to have the function of parallel computing and run on parallel computing facility based on message passing interface (MPI). The coupled software is used to simulate the combustion process of spark ignition CNG engine. The results show that sim ulation data have a good consistency with experimental results and parallel computing has good effi ciency and accelerate ratio.展开更多
基金financially supported by the National Natural Science Foundation of China (Grant no.41076130)the SOA Youth Marine Science Foundation (Grant no.2010116)the Open Research Foundation of Laboratory of Marine Ecosystem and Biogeochemistry,SOA (Grant no.LMEB200902)
文摘In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, rates of primary production and physiological status. This study describes a preliminary investigation on the joint application of the three kinds of fluorescence analysis in the physiological study of microalgae. Flow cytometry and fluorescence spectrometry were used to obtain the in vivo static fluorescence information of pigments, and a Pulsed-Amplitude-Modulation chlorophyll fluorometer was used to detect the dynamic fluorescence of chlorophyll. The validity of the joint application was proved by analyzing two labora- tory cultured Arctic microalgae, Pseudo-nitzschia delicatissima (Bacillariophyceae) and Thalassiosira sp. The higher value of minimum fluorescence yield in dark-adapted state (Fo), actual photochemical efficiency of PSll (ФPSII), and electron transport rate (ETR) exhibited positive results in a higher cell abundance and chlorophyll a content of P. delicatissima; whereas higher fl-carotene content of Thalassiosira sp. played an important role in the protection of photosynthesis.
基金Supported by the National Natural Science Foundation of China(50976012)
文摘A three dimensional model which considers the effects of turbulence and detailed chemi cal kinetics is built to simulate the combustion process of engine fueled by compressed nature gas (CNG). The model is accomplished by integrating CFD software KIVA3V and chemical kinetic soft- ware CHEMKINII. Meanwhile, a turbulence combustion model which is suitable for describing the reaction rate under the coupled simulation is developed to balance the effects of turbulence and de tailed chemical kinetics. To reduce the computation time, subsequent development of the simulation code is realized, which enables the simulation code to have the function of parallel computing and run on parallel computing facility based on message passing interface (MPI). The coupled software is used to simulate the combustion process of spark ignition CNG engine. The results show that sim ulation data have a good consistency with experimental results and parallel computing has good effi ciency and accelerate ratio.