目的:采用高效液相色谱法测定化合物ent-kaur-16-en-19-oic ac id在光叶番荔枝不同部位和番荔枝属不同种类植物叶子中的含量。方法:色谱条件为:Nuc leosil C18色谱柱,流动相选用乙睛:水(含1%冰醋酸)=67.5:32.5,检测波长211nm。结果:平...目的:采用高效液相色谱法测定化合物ent-kaur-16-en-19-oic ac id在光叶番荔枝不同部位和番荔枝属不同种类植物叶子中的含量。方法:色谱条件为:Nuc leosil C18色谱柱,流动相选用乙睛:水(含1%冰醋酸)=67.5:32.5,检测波长211nm。结果:平均回收率为98.41%,光叶番荔枝树皮、光叶番荔枝树枝(去皮)、光叶番荔枝树叶、AP番荔枝树叶、牛心番荔枝树叶、山刺番荔枝树叶中化合物ent-kaur-16-en-19-oic ac id的含量分别为0.7147%、0.01252%、0.01773%、0.04212%、0.007305%、0.09083%。结论:在光叶番荔枝各部位中,树皮的含量最高,达0.7147%,在所测番荔枝属植物的树叶中,山刺番荔枝树叶中含量最高,达0.09083%。展开更多
A new diterpenoid dimer annonebinide A has been isolated from the stems of Annona glabra. Its structure was determined to be ent-16a-hydroxykauran-17-yl ent-16b-kauran-17-oate on the basis of spectroscopic and chemic...A new diterpenoid dimer annonebinide A has been isolated from the stems of Annona glabra. Its structure was determined to be ent-16a-hydroxykauran-17-yl ent-16b-kauran-17-oate on the basis of spectroscopic and chemical evidence.展开更多
A new ent-kaurane diterpene was isolated from the rhizome of Aralia fargesii. On the basis of chemical and spectral evidences, its structure was established as 16 alpha-hydroxy-17-isovaleroyloxy-ent-kauran-19-oic acid.
目的:探讨Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid(5F)对人绒癌JAR细胞侵袭、转移、黏附的影响及MMP-9、VEGF表达的变化。方法:用不同浓度的5F作用JAR细胞,MTT比色法,Transwell小室,细胞黏附实验观察其增殖、侵袭、迁移、黏附...目的:探讨Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid(5F)对人绒癌JAR细胞侵袭、转移、黏附的影响及MMP-9、VEGF表达的变化。方法:用不同浓度的5F作用JAR细胞,MTT比色法,Transwell小室,细胞黏附实验观察其增殖、侵袭、迁移、黏附能力的变化;Western blot方法检测5F对JAR细胞MMP-9、VEGF表达水平的影响。结果:作用6 h,5F能抑制JAR细胞的增殖、转移、侵袭、黏附,其抑制率呈剂量效应(P<0.05)。5F作用24 h JAR细胞,MMP-9、VEGF蛋白表达水平能明显下调。结论:5F能抑制JAR细胞的侵袭、转移、黏附并下调MMP-9、VEGF蛋白表达水平。展开更多
In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance（NMR） tech- ...In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance（NMR） tech- niques and mass spectrometry and identified to be 3a-phenylpropionoyloxy-ent-kaur-16-en-oic acid（l）. The isolated diterpenes were tested for their cytotoxicity activities via 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bro- mide（MTT） assay. The results show that compounds 1, 2, 3, 4 and 6 exhibit different levels of cytotoxic activities. Especially, compound 2 shows significant cytotoxicity toward HeLa and A549 cell lines（IC50=6.14 and 8.76 μmol/L）.展开更多
Two new diterpenoids,3,4,18β-cyclopropa-ent-abieta-8（14）,13（15）-dien-16,12-olide（1） and 3-oxojolkinolide B（2）,were isolated from the roots of Suregada glomerulata（Blume） Baill.Their structures were determin...Two new diterpenoids,3,4,18β-cyclopropa-ent-abieta-8（14）,13（15）-dien-16,12-olide（1） and 3-oxojolkinolide B（2）,were isolated from the roots of Suregada glomerulata（Blume） Baill.Their structures were determined by spectroscopic evidences. Compound 2 showed moderate cytotoxicity.展开更多
文摘目的:采用高效液相色谱法测定化合物ent-kaur-16-en-19-oic ac id在光叶番荔枝不同部位和番荔枝属不同种类植物叶子中的含量。方法:色谱条件为:Nuc leosil C18色谱柱,流动相选用乙睛:水(含1%冰醋酸)=67.5:32.5,检测波长211nm。结果:平均回收率为98.41%,光叶番荔枝树皮、光叶番荔枝树枝(去皮)、光叶番荔枝树叶、AP番荔枝树叶、牛心番荔枝树叶、山刺番荔枝树叶中化合物ent-kaur-16-en-19-oic ac id的含量分别为0.7147%、0.01252%、0.01773%、0.04212%、0.007305%、0.09083%。结论:在光叶番荔枝各部位中,树皮的含量最高,达0.7147%,在所测番荔枝属植物的树叶中,山刺番荔枝树叶中含量最高,达0.09083%。
文摘A new diterpenoid dimer annonebinide A has been isolated from the stems of Annona glabra. Its structure was determined to be ent-16a-hydroxykauran-17-yl ent-16b-kauran-17-oate on the basis of spectroscopic and chemical evidence.
文摘A new ent-kaurane diterpene was isolated from the rhizome of Aralia fargesii. On the basis of chemical and spectral evidences, its structure was established as 16 alpha-hydroxy-17-isovaleroyloxy-ent-kauran-19-oic acid.
文摘目的:探讨Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid(5F)对人绒癌JAR细胞侵袭、转移、黏附的影响及MMP-9、VEGF表达的变化。方法:用不同浓度的5F作用JAR细胞,MTT比色法,Transwell小室,细胞黏附实验观察其增殖、侵袭、迁移、黏附能力的变化;Western blot方法检测5F对JAR细胞MMP-9、VEGF表达水平的影响。结果:作用6 h,5F能抑制JAR细胞的增殖、转移、侵袭、黏附,其抑制率呈剂量效应(P<0.05)。5F作用24 h JAR细胞,MMP-9、VEGF蛋白表达水平能明显下调。结论:5F能抑制JAR细胞的侵袭、转移、黏附并下调MMP-9、VEGF蛋白表达水平。
基金The project of State Key Laboratory of Phytochemistry and Plant Resourcesin West China (P-06-04) Kunming Institute of Botany,CAS,and the 973 Program(No.2009CB522300)
基金Supported by the National Natural Science Foundation of China（No.31300285）, the Natural Science Foundation of Fujian Province, China（No.2015J01348）, the Specialized Research Fund for the Doctoral Program of Higher Education of China （No.20110073120072） and the Fujian Province Development Guidance（Key） Project, China（No.2014Y0074）.
文摘In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance（NMR） tech- niques and mass spectrometry and identified to be 3a-phenylpropionoyloxy-ent-kaur-16-en-oic acid（l）. The isolated diterpenes were tested for their cytotoxicity activities via 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bro- mide（MTT） assay. The results show that compounds 1, 2, 3, 4 and 6 exhibit different levels of cytotoxic activities. Especially, compound 2 shows significant cytotoxicity toward HeLa and A549 cell lines（IC50=6.14 and 8.76 μmol/L）.
基金the National Science and Technology Project of China(No.2009ZX09301-003-12-1) for financial support of this work
文摘Two new diterpenoids,3,4,18β-cyclopropa-ent-abieta-8（14）,13（15）-dien-16,12-olide（1） and 3-oxojolkinolide B（2）,were isolated from the roots of Suregada glomerulata（Blume） Baill.Their structures were determined by spectroscopic evidences. Compound 2 showed moderate cytotoxicity.
