Four new ent-kaurane diterpenoids,namely,3α-tigloyloxypterokaurene L_(3)(1),ent-17-hydroxy-kaura-9(11),15-dien-19-oic acid(2),and wedelobatins A(3)and B(4),together with 11 known ent-kaurane diterpenoids(5-15),were i...Four new ent-kaurane diterpenoids,namely,3α-tigloyloxypterokaurene L_(3)(1),ent-17-hydroxy-kaura-9(11),15-dien-19-oic acid(2),and wedelobatins A(3)and B(4),together with 11 known ent-kaurane diterpenoids(5-15),were isolated from the ethanol extract of Wedelia trilobata.All the structures of 1-15 were elucidated on the basis of spectroscopic studies.展开更多
Two new ent-kaurane diterpenoids, xerophilusin E (1) and xerophilusin F (2), were isolated from the leaves of Isodon xerophilus. Their structures were determined as 3, 20: 7, 20-diepoxy-ent-kaur-16-en-15-one and 7 bet...Two new ent-kaurane diterpenoids, xerophilusin E (1) and xerophilusin F (2), were isolated from the leaves of Isodon xerophilus. Their structures were determined as 3, 20: 7, 20-diepoxy-ent-kaur-16-en-15-one and 7 beta, 14 beta, 20 (R)-trihydroxy-11 beta-acetoxy-7,20-cyclo-ent-kaur-16-en-6, 15-dione, respectively, by spectral methods and X-ray crystallographic analysis.展开更多
Two new ent-kaurane diterpenoids, minheryins H (1) and I (2), were isolated from the leaves of Isodon henryi. Their structures were elucidated by means of spectroscopic analysis, including 2D NMR spectra.
Three new ent-kauranoids,isorosthornins A-C(1-3),and a new natural product,dihydroponicidin(4),together with five known ones were isolated from the aerial parts of Isodon rosthornii.The structures were determined by m...Three new ent-kauranoids,isorosthornins A-C(1-3),and a new natural product,dihydroponicidin(4),together with five known ones were isolated from the aerial parts of Isodon rosthornii.The structures were determined by means of extensive spectroscopic analysis.All diterpenoids isolated were evaluated for their cytotoxicity against HL-60,SMMC-7721,A-549,MCF-7,and SW480 cell lines,and compounds 5 and 7 showed significant inhibitory effects on all cell lines.展开更多
Two new ent-kaurane diterpenoids taihangexcisoidesin A and B (1 and 2), were isolated from the EtOAc extract of the leaves of lsodon excisoides. Their structures were determined on the basis of spectroscopic methods.
Five new ent-kaurane diterpenoids,named mascaroside Ⅲ–Ⅴ(1–3),and 20-nor-cofaryloside Ⅰ–Ⅱ(4–5),together with seven known diterpenoids,were isolated from methanol extracts of the green coffee beans of Yunnan Ara...Five new ent-kaurane diterpenoids,named mascaroside Ⅲ–Ⅴ(1–3),and 20-nor-cofaryloside Ⅰ–Ⅱ(4–5),together with seven known diterpenoids,were isolated from methanol extracts of the green coffee beans of Yunnan Arabica Coffee.Their chemical structures were elucidated by extensive spectroscopic analyses.Meanwhile,cytotoxicity assay against HL-60,A-549,SMMC-7721,MCF-7 and SW480 cell lines showed that they have not evident inhibition of cytotoxicity.展开更多
A new spirosecokaurenoid, enanderinanin F 1 and its C-6 epimer, angustifolin were isolated from the aerial parts of Isodon enanderianus. The structure of 1 was determined on the basis of spectral data, especially by 2...A new spirosecokaurenoid, enanderinanin F 1 and its C-6 epimer, angustifolin were isolated from the aerial parts of Isodon enanderianus. The structure of 1 was determined on the basis of spectral data, especially by 2D techniques. The C-13 NMR data of angustifolin were revised by 2D NMR.展开更多
Four new ent-kaurane diterpenoids, 6fl,13ct,15fl-trihydroxy-16-ene-3a,20-epoxy-ent-kaur- 1,7-dione (1), 6-hydroxy-3a,20-epoxy-5(6)-ene-ent-kaur-l,7,15-trione (2), 6-hydroxy-15fl-acetoxy-3a,20-epoxy-16]3,17-epoxy...Four new ent-kaurane diterpenoids, 6fl,13ct,15fl-trihydroxy-16-ene-3a,20-epoxy-ent-kaur- 1,7-dione (1), 6-hydroxy-3a,20-epoxy-5(6)-ene-ent-kaur-l,7,15-trione (2), 6-hydroxy-15fl-acetoxy-3a,20-epoxy-16]3,17-epoxy- 5(6)-ene-ent-kaur-l,7-dione (3), 3ct,17-dihydroxy-15(16)-ene-ent-kaur-7-one (4), along with four known com- pounds 5--8 were isolated from the leaves oflsodon eriocalyx var. laxiflora. The structures were elucidated by ex- tensive spectroscopic methods (IR, UV, MS and NMR). The cytotoxic activities of these compounds were evaluated by MTT assay. Compound 8 showed moderate inhibitory effects on HL-60, SMMC-7721, MCF-7 and SW-480 cell lines.展开更多
Two new ent-kaurane diterpenoids, ent-7α,14β-dihydroxykaur-16-en-15-one-20-oic acid (1) and 1,7α,12β,14β-tetrahydroxy-1,10-seco-ent-kaur-10,16-dien-15-one (2), together with six known ones (3-8), were isola...Two new ent-kaurane diterpenoids, ent-7α,14β-dihydroxykaur-16-en-15-one-20-oic acid (1) and 1,7α,12β,14β-tetrahydroxy-1,10-seco-ent-kaur-10,16-dien-15-one (2), together with six known ones (3-8), were isolated from the EtOAc extract of the aerial parts of lsodon excisoides. Their structures were elucidated on the basis of 1D NMR and 2D NMR analyses as well as HR-ESI-MS experiments.展开更多
One new 6,7-seco-ent-kaurane diterpenoid, sculponin T (1), was isolated from the aerial parts of lsodon sculponeatus, along with four known analogs, sculponeatin J (2), sculponeatin K (3), sculponeatin C (4), ...One new 6,7-seco-ent-kaurane diterpenoid, sculponin T (1), was isolated from the aerial parts of lsodon sculponeatus, along with four known analogs, sculponeatin J (2), sculponeatin K (3), sculponeatin C (4), and sculponeatin Q (5). Their structures were elucidated by extensive spectroscopic analysis and by comparison with data reported in the literature. Significant cytotoxic activity was observed for compound 2 against five human tumor cell lines with IC50 values ranging from 1.8 μmol/L to 3.3 μmol/L, and it also inhibited NO production in LPS-stimulated RAW264.7 cells, with IC50 value of 3.3 μmol/L.展开更多
Three new ent-kaurane diterpenoids fimbrialtols K–M(3–5) with highly substituted functionalities were isolated from the extract of Flickingeria fimbriata(B1.) Hawkes. The structures of these new compounds were d...Three new ent-kaurane diterpenoids fimbrialtols K–M(3–5) with highly substituted functionalities were isolated from the extract of Flickingeria fimbriata(B1.) Hawkes. The structures of these new compounds were determined by NMR and HR-ESIMS. The relative configurations of these compounds were determined by analysis of NOESY correlations. The absolute configurations of these new compounds were established by CD methods together with considering the biosynthetic pathway. Compounds 3 and4 contain a cinnamic carboxyl group, whereas compound 5 possesses a benzoic carboxyl group representing the first report in more than known 600 ent-kaurane diterpenoids.展开更多
Nine new ent-kaurane diterpenoids, named scopariusols L–T(1–9), were isolated from the aerial parts of Isodon scoparius. Their structures were characterized mainly by analyzing the NMR and HR-ESI-MS data, and the ab...Nine new ent-kaurane diterpenoids, named scopariusols L–T(1–9), were isolated from the aerial parts of Isodon scoparius. Their structures were characterized mainly by analyzing the NMR and HR-ESI-MS data, and the absolute configuration of 1 was determined by single-crystal X-ray diffraction. Compound 1 was active against five human tumor cell lines(HL-60, SMMC-7721, A-549, MCF-7, and SW-480), and it also inhibited NO production in LPS-stimulated RAW264.7 cells, with an IC50 value of 0.6 μmol·L-1.展开更多
In this study, ent-Kaurane-3β,16β,17-trioi (1) and ent-kaurane-2a,16β,17-triol (2), were isolated from the leaves ofRubus corchorifolius L. f. Their structures were elucidated based on extensive spectroscopic a...In this study, ent-Kaurane-3β,16β,17-trioi (1) and ent-kaurane-2a,16β,17-triol (2), were isolated from the leaves ofRubus corchorifolius L. f. Their structures were elucidated based on extensive spectroscopic analysis. NMR experiments identified the two compounds and X-ray crystallographic analysis confirmed their crystal structures. The crystal of 1 belongs to monoclinic with space group P21 and a = 10.7641(3), b = 7.2789(3), c = 11.7862(4) A, β = 95.275(3)°, V = 919.55(6) A3, Z = 2, C20H34O4, Mr = 322.49 g/mol, Dc = 1.230 g/m3, F(000) = 376, 2 = 1.54178 A,μ = 0.661 mm-1, R = 0.0319, and wR = 0.0811 for 10889 observed reflections (I〉 2σ(I)). The crystal of 2 is classified as orthorhombic with space group P212121 and a = 10.7641(3), b = 12.72224(19), c = 21.3929(3) A, V= 1748.94(4) A3, Z= 4, C20H3404, Mr = 322.47, De = 1.225 Mg/m3 F(000) = 712, λ = 1.54184 A,μ = 0.625 mm-1, R = 0.0303 and wR = 0.0759 for 10470 observed reflections (1〉 2σ(I)). Meanwhile, the compound revealed low inhibitory activities toward HepG2, MCF-7, and SCG-7901 cells.展开更多
基金The authors acknowledge the National Basic Research Program of China(973 Program,2009CB522300)the“West Light”program of Chinese Academy of Sciences.
文摘Four new ent-kaurane diterpenoids,namely,3α-tigloyloxypterokaurene L_(3)(1),ent-17-hydroxy-kaura-9(11),15-dien-19-oic acid(2),and wedelobatins A(3)and B(4),together with 11 known ent-kaurane diterpenoids(5-15),were isolated from the ethanol extract of Wedelia trilobata.All the structures of 1-15 were elucidated on the basis of spectroscopic studies.
基金supported financially by the NSFC(No.30772637 to H.-D.Sun)the NSFC-joint Foundation of Yunnan Province(No.U0832602 to H.-D.Sun)+2 种基金the Major State Basic Research Development Program of China(No. 2009CB522303 and 2009CB940900)the Natural Science Foundation of Yunnan Province(No.2008CD 162)the Key Project of Knowledge Innovation Project of CAS(No.KSCX2-YW-R-25).
文摘Two new ent-kaurane diterpenoids, xerophilusin E (1) and xerophilusin F (2), were isolated from the leaves of Isodon xerophilus. Their structures were determined as 3, 20: 7, 20-diepoxy-ent-kaur-16-en-15-one and 7 beta, 14 beta, 20 (R)-trihydroxy-11 beta-acetoxy-7,20-cyclo-ent-kaur-16-en-6, 15-dione, respectively, by spectral methods and X-ray crystallographic analysis.
基金the Natural Science Foundation of Yunnan Province (No. 2004C0008Z)the National Natural Science Foundation of China (No.20502026 to Q.B.H.and No.30772637 to H.D.Sun).
文摘Two new ent-kaurane diterpenoids, minheryins H (1) and I (2), were isolated from the leaves of Isodon henryi. Their structures were elucidated by means of spectroscopic analysis, including 2D NMR spectra.
基金This work was supported financially by the NSFC-Joint Foundation of Yunnan Province(No.U0832602 to H.D.Sun)the Major State Basic Research Development Program of China(No.2009CB522300 and 2009CB940900)+2 种基金the Science and Technology Program of Yunnan Province(No.2008IF010 and 2008CD162)the NSFC(No.81172939 to J.X.Pu)the Major Direction Projection Foundation of CAS Intellectual Innovation Project(No.2010KIBA05 to J.X.Pu).
文摘Three new ent-kauranoids,isorosthornins A-C(1-3),and a new natural product,dihydroponicidin(4),together with five known ones were isolated from the aerial parts of Isodon rosthornii.The structures were determined by means of extensive spectroscopic analysis.All diterpenoids isolated were evaluated for their cytotoxicity against HL-60,SMMC-7721,A-549,MCF-7,and SW480 cell lines,and compounds 5 and 7 showed significant inhibitory effects on all cell lines.
基金The authors wish to thank all members of the analytical group of Phytochemistry Laboratory of Kunming Institute of Botany Academic Sinica for the measurement of spectral data.
文摘Two new ent-kaurane diterpenoids taihangexcisoidesin A and B (1 and 2), were isolated from the EtOAc extract of the leaves of lsodon excisoides. Their structures were determined on the basis of spectroscopic methods.
基金This work was supported financially by Programme of Key New Productions of Yunnan Province,Centre of CHINA(No.2015BB002)The STS Programme of Chinese Academy of Sciences(KFJ-SW-STS-143-8)as well as Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2015-ZZ09).
文摘Five new ent-kaurane diterpenoids,named mascaroside Ⅲ–Ⅴ(1–3),and 20-nor-cofaryloside Ⅰ–Ⅱ(4–5),together with seven known diterpenoids,were isolated from methanol extracts of the green coffee beans of Yunnan Arabica Coffee.Their chemical structures were elucidated by extensive spectroscopic analyses.Meanwhile,cytotoxicity assay against HL-60,A-549,SMMC-7721,MCF-7 and SW480 cell lines showed that they have not evident inhibition of cytotoxicity.
文摘A new spirosecokaurenoid, enanderinanin F 1 and its C-6 epimer, angustifolin were isolated from the aerial parts of Isodon enanderianus. The structure of 1 was determined on the basis of spectral data, especially by 2D techniques. The C-13 NMR data of angustifolin were revised by 2D NMR.
文摘Four new ent-kaurane diterpenoids, 6fl,13ct,15fl-trihydroxy-16-ene-3a,20-epoxy-ent-kaur- 1,7-dione (1), 6-hydroxy-3a,20-epoxy-5(6)-ene-ent-kaur-l,7,15-trione (2), 6-hydroxy-15fl-acetoxy-3a,20-epoxy-16]3,17-epoxy- 5(6)-ene-ent-kaur-l,7-dione (3), 3ct,17-dihydroxy-15(16)-ene-ent-kaur-7-one (4), along with four known com- pounds 5--8 were isolated from the leaves oflsodon eriocalyx var. laxiflora. The structures were elucidated by ex- tensive spectroscopic methods (IR, UV, MS and NMR). The cytotoxic activities of these compounds were evaluated by MTT assay. Compound 8 showed moderate inhibitory effects on HL-60, SMMC-7721, MCF-7 and SW-480 cell lines.
基金supported by the opening fund of Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation,Huazhong University of Science and Technology
文摘Two new ent-kaurane diterpenoids, ent-7α,14β-dihydroxykaur-16-en-15-one-20-oic acid (1) and 1,7α,12β,14β-tetrahydroxy-1,10-seco-ent-kaur-10,16-dien-15-one (2), together with six known ones (3-8), were isolated from the EtOAc extract of the aerial parts of lsodon excisoides. Their structures were elucidated on the basis of 1D NMR and 2D NMR analyses as well as HR-ESI-MS experiments.
基金supported financially by the National Natural Science Foundation of China(Nos.21322204 and 81172939)the NSFC-Joint Foundation of Yunnan Province(No.U1302223)+1 种基金the Reservation-talent Project of Yunnan Province(No.2011CI043)the West Light Foundation of the Chinese Academy of Sciences(Jian-Xin Pu)
文摘One new 6,7-seco-ent-kaurane diterpenoid, sculponin T (1), was isolated from the aerial parts of lsodon sculponeatus, along with four known analogs, sculponeatin J (2), sculponeatin K (3), sculponeatin C (4), and sculponeatin Q (5). Their structures were elucidated by extensive spectroscopic analysis and by comparison with data reported in the literature. Significant cytotoxic activity was observed for compound 2 against five human tumor cell lines with IC50 values ranging from 1.8 μmol/L to 3.3 μmol/L, and it also inhibited NO production in LPS-stimulated RAW264.7 cells, with IC50 value of 3.3 μmol/L.
基金financially supported by the Chinese National S&T Special Project on Major New Drug Innovation(No.2013ZX09508104)the Fundamental Research Funds for the Central Scientific Research Institutes for Public WelfareCAMS Innovation Fund for Medical Sciences(CIFMS,No.2016-I2M-3-015)
文摘Three new ent-kaurane diterpenoids fimbrialtols K–M(3–5) with highly substituted functionalities were isolated from the extract of Flickingeria fimbriata(B1.) Hawkes. The structures of these new compounds were determined by NMR and HR-ESIMS. The relative configurations of these compounds were determined by analysis of NOESY correlations. The absolute configurations of these new compounds were established by CD methods together with considering the biosynthetic pathway. Compounds 3 and4 contain a cinnamic carboxyl group, whereas compound 5 possesses a benzoic carboxyl group representing the first report in more than known 600 ent-kaurane diterpenoids.
基金supported by the National Natural Science Foundation of China(No.21322204)the NSFC-Joint Foundation of Yunnan Province(No.U1302223)
文摘Nine new ent-kaurane diterpenoids, named scopariusols L–T(1–9), were isolated from the aerial parts of Isodon scoparius. Their structures were characterized mainly by analyzing the NMR and HR-ESI-MS data, and the absolute configuration of 1 was determined by single-crystal X-ray diffraction. Compound 1 was active against five human tumor cell lines(HL-60, SMMC-7721, A-549, MCF-7, and SW-480), and it also inhibited NO production in LPS-stimulated RAW264.7 cells, with an IC50 value of 0.6 μmol·L-1.
基金financially supported by the National Science and Technology Pillar Program during the Twelfth Five-year Plan Period(2012BAK17B00)the Doctoral Program of Higher Education,China(No.20114404120022)the Agricultural Science and Technology Planned Projects of Guangzhou,China(No.2012A020602038)
文摘In this study, ent-Kaurane-3β,16β,17-trioi (1) and ent-kaurane-2a,16β,17-triol (2), were isolated from the leaves ofRubus corchorifolius L. f. Their structures were elucidated based on extensive spectroscopic analysis. NMR experiments identified the two compounds and X-ray crystallographic analysis confirmed their crystal structures. The crystal of 1 belongs to monoclinic with space group P21 and a = 10.7641(3), b = 7.2789(3), c = 11.7862(4) A, β = 95.275(3)°, V = 919.55(6) A3, Z = 2, C20H34O4, Mr = 322.49 g/mol, Dc = 1.230 g/m3, F(000) = 376, 2 = 1.54178 A,μ = 0.661 mm-1, R = 0.0319, and wR = 0.0811 for 10889 observed reflections (I〉 2σ(I)). The crystal of 2 is classified as orthorhombic with space group P212121 and a = 10.7641(3), b = 12.72224(19), c = 21.3929(3) A, V= 1748.94(4) A3, Z= 4, C20H3404, Mr = 322.47, De = 1.225 Mg/m3 F(000) = 712, λ = 1.54184 A,μ = 0.625 mm-1, R = 0.0303 and wR = 0.0759 for 10470 observed reflections (1〉 2σ(I)). Meanwhile, the compound revealed low inhibitory activities toward HepG2, MCF-7, and SCG-7901 cells.