Soil conditioners improve Cd-contaminated farmland soil microbial communities to inhibit Cd accumulation in rice

The addition of silicon(Si)and organic fertilizers to soil conditioners can inhibit the transfer of heavy metal ions from soil to crops.However,it is not clear how Si and organic fertilizers affect soil properties and the micro-ecological environment and thereby reduce cadmium(Cd)accumulation in rice.In this study,the effects of L-type soil conditioners containing Si and organic fertilizers on bacterial and fungal community diversity,soil pH,organic matter,and available Si were analyzed with field experiments at two sites in Liuzhou City and Hezhou City,respectively,in Guangxi,China.With the increase of Si and organic fertilizer content in soil conditioner,rice yield respectively increased by 16.8–25.8 and 6.8–13.1%,and rice Cd content decreased significantly by 8.2–21.1 and 10.8–40.6%,respectively,at the two experimental sites.Soil microbiome analysis showed that the increase in abundance of Firmicutes and Actinobacteriota bacteria associated with Cd adsorption and sequestration,and Basidiomycota fungal populations associated with degradation of macromolecules favored the inhibition of soil Cd activity(soil exchangeable Cd decreased by 14.4–14.8 and 18.1–20.6%).This was associated with an increase in organic matter and Si content caused by applying soil conditioners.In conclusion,L-type soil conditioners,rich in Si and organic fertilizer,can reduce soil Cd bioavailability by regulating the dominant Cd passivating flora in the soil and ultimately reduce Cd accumulation in rice.

Control Effects of Tianshifu Soil Conditioners on Cd Contamination in Paddy Fields of Hunan Province

To investigate the effects of Tianshifu soil conditioners on rice growth and their control effects on Cd absorption and accumulation in rice, a test was carded out in the field typically polluted by Cd in Hunan Province. The results showed that the Tianshifu soil conditioner, soil bacterial conditioner and the combination of Tian- shifu soil conditioner and soil bacterial conditioner all could promote rice growth, im- prove rice yield and reduce significantly Cd content in rice grains. In Beishan, the Cd contents in rice grains treated by Tianshifu soil conditioner, soil bacterial condi- tioner and the combination of Tianshifu soil conditioner and soil bacterial conditioner were reduced by 37.63% （P〈0.05）, 32.59% （P〈0.05） and 27.95% （P〈0.05） respec- tively compared with that in rice grains treated by conventional fertilization; In Xiang- tan, the Cd contents in rice grains were reduced by 54.36% （P〈0.05）, 50.19% （P〈 0.05） and 45.22% （P〈0.05） respectively. The rice grains harvested at the two test sites all met the national food safety standards. Considering the control effect on Cd contamination, Tianshifu soil conditioner 〉 combination of Tianshifu soil condi- tioner and soil bacterial conditioner 〉 Tianshifu soil bacterial conditioner. In addition to reducing Cd absorption in rice, as described in the lime application treatment, the application of Tianshifu soil conditioners also inhibited the transportation of Cd from rice straws to rice grains. The Tianshifu soil conditioner, soil bacterial conditioner and combination of Tianshifu soil conditioner and soil bacterial conditioner also effec- tively improved the soil acidity, increased the soil pH value and reduced soil avail- able Cd content to some extent.

Effects of Multiple Soil Conditioners on a Mine Site Acid Sulfate Soil for Vetiver Growth

A pot experiment was conducted to investigate the effects of various soil treatments on the growth of vetiver grass ( Vetiveria zizanioides (L.) Nash) with the objective of formulating appropriate soil media for use in sulfide-bearing mined areas. An acidic mine site acid sulfate soil (pH 2.8) was treated with different soil conditioner formula including hydrated lime, red mud (bauxite residues), zeolitic rock powder, biosolids and a compound fertilizer. Soils treated with red mud and hydrated lime corrected soil acidity and reduced or eliminated metal toxicity enabling the establishment of vetiver grass.Although over-liming affected growth, some seedlings of vetiver survived the initial strong alkaline conditions. Addition of appropriate amounts of zeolitic rock powder also enhanced growth, but over-application caused detrimental effects. In this experiment, soil medium with the best growth performance of vetiver was 50 g of red mud, 10 g of lime, 30 g of zeolitic rock powder and 30 g of biosolids with 2000 g of mine soils (100% survival rate with the greatest biomass and number of new shoots), but adding a chemical fertilizer to this media adversely impacted plant growth. In addition, a high application rate of biosolids resulted in poorer growth of vetiver, compared to a moderate application rate.

Characteristics of root-permeated soil under simple-shear and direct-shear conditions

The simple-shear condition is closer to reality than the direct-shear condition for simulating the mechanical behavior of vegetated soil slope under shallow failure.However,study on simple-shear characteristics for vegetated slope is still insufficient,and there lacks intuitive comparison of characteristics between these two shear conditions.In this study,large-scale simple-shear and direct-shear experiments were conducted on soil permeated by roots of Amorpha fruticosa to investigate the shear strength and stiffness.The stress-displacement relationship of each sample was obtained and further normalized to unify the influence of root content.The results reveal that the direct-shear condition overestimates the shear strength of root-permeated soils(by 41%)and thus the estimation of slope stability based on the parameters of direct-shear condition is not conservative.Furthermore,the initial stiffness of root-permeated soil under simple-shear condition is 34%lower than that under direct-shear condition.The higher strength and stiffness under direct-shear condition are caused by the following reasons:the shear plane does not have the lowest strength,the shear area is decreasing,and the shear zone is thinner.The significant deformation(lower stiffness)revealed by the simple-shear condition facilitates the application of early warning for vegetated shallow landslides.

Influences of Oyster Shell Soil Conditioner on Soil and Plant Rhizospheric Microorganisms

Oyster shell soil conditioner had significant influence on soil and rhizospheric microorganisms in their biomass,respiratory intensity and nutritional requirement. It could stimulate growth of soil and rhizospheric microorganisms, especially nitrogen-fixers, and intensify soil respiration in proportion to the dose and fertilizing time of the conditioner, leading to the increase in the number of nitrogen fixing bacteria and the decrease in the number of bacteria with special nutrition demands.

Remediation Effects of Soil Conditioners for CdPolluted Paddy Fields

A field experiment was conducted to evaluate the remediation effects of 14 soil conditioners for Cd-polluted paddy fields contrasted with conventional fertilization and liming.The results showed that soil conditioners had no significant impact on rice yields.Though the soil conditioners had stable effects on decreasing the Cd content in grains,the effects varied with the category of conditioners.In general,The Cd contents of early rice and late rice were reduced by 22.65%~44.24%and 15.20%~63.03%,respectively.Additionally,the stem and leaves showed the same decreasing tendency.Cluster analysis suggested that soil conditioners can be divided into three categories:the first category increases the soil pH to inhibit the Cd activity and cut down the Cd translocation from soil to stem and leaves,which is most efficient in the reduction of Cd content in rice grains;the other category relies on the antagonism or co-precipitation between Cd and certain elements to inhibit the translocation and redistribution of Cd from rice stems to grains,providing a common effect on Cd reduction;the last category can be seen as a combination of the former two categories from the perspective of its functional mechanism and it has a moderate effect on the reduction of Cd contents in rice grains.

Effect of Soil Conditioner on the Microbial Community in the Rhizosphere Soil of Tobacco

The effects of biochar as a soil conditioner on microbial communities were studied. The results showed that, at all the growth stages of tobacco, the quantity of microorganisms in the rhizosphere soil added with the soil conditioner was higher than control （CK）. The results of metagenome detection indicated that the diversity of microbial communities in soil added with the soil conditioner was also higher than the CK, and the rate of tobacco bacterial wilt and the disease index decreased by 27.6% and 34.6%, respectively, compared with the CK. In conclusion, the soil con- ditioner could reduce the soil-borne disease by improving micro-ecological environ- ment and diversity of microbial communities in rhizosphere soil to maintain the soil balance.

Anaerobic Mono-Digestion of Turkey Manure: Efficient Revaluation to Obtain Methane and Soil Conditioner

This work demonstrates the possibility to make a full valuation of a solid waste such as turkey manure, to obtain methane and a soil conditioner/fertilizer from turkey manure anaerobic digestion in a mesophilic pilot-scale continuous stirred tank reactor at different organic loading rates (OLR) (from 0.5 to 2.5 kgVS/m3d). The application of the anaerobic mono-digestion for the turkey manure treatment was an efficient alternative, because high volatile solids removal and methane were obtained in addition to obtaining a stabilized solid waste that can be applied as soil conditioner, based on its nutritional parameters and humic substances content. In this way, the turkey manure anaerobic digestion can be applied avoiding the co-digestion of the manure with other wastes and allows a process devoid of pollutant emissions, obtaining two products. The reactor operation depends on the OLR, and its operation does not allow an OLR above 1.5 kgVS/m3d. Higher OLR produced a decrease in the TS and VS removals and methane productivity.

Remediation Effects of a Novel Soil Conditioner onCadmium and Arsenic Contaminated Soil in Early-Season Paddy Field

To investigate the effects of“Runbang”soil conditioner on simultaneously decreasing cadmium(Cd)and arsenic(As)in early-season paddy field,a typical red mud field in Hunan Province was selected for monitoring the dynamic changes of Cd and As contents in early-season rice plants and soils,plus the soil physical-chemical properties and rice yields.Results of the field experiments indicated that the soil conditioner could significantly reduce Cd and As contents in soil and rice grain,whereas it could increase rice yield.Applying Runbang soil conditioner(1500~2100 kg/hm^2)to paddies brought about a reduction of 23.7%~44.8% and 24.3%~40.5% in available Cd and As contents of the soil,an increase of 0.9~1.1 units in soil pH value,a decline of 35.6%~51.1% and 22.0%~40.6% in Cd and As contents of rice grains,and a rise of 2%~5% in rice yields,respectively.

Study on the Passivation Effect of Ca-Si Soil Conditioner on Heavy Metal Absorption by Rice

[Objectives] In order to solve the problem of heavy metal pollution in paddy fields,field experiments were carried out to study the effect of Ca-Si soil conditioner on heavy metal remediation in paddy fields. [Methods] A conventional planting mode( CK) was used as the control,and a Ca-Si soil conditioner( Ca-Si) treatment was set to analyze the differences in soil pH and heavy metal contents in different treatments. [Results]The content of cadmium in the paddy field exceeded the soil pollution risk control value of agricultural land by 59. 33%. The Ca-Si soil conditioner increased the pH value of paddy field soil by 0. 58 unit. The fixation rates of Ca-Si soil conditioner on chromium,arsenic,cadmium and plumbum reached 75. 96%,14. 09%,18. 93% and 7. 81% compared with the CK,respectively,and the available cadmium and lead contents were reduced by 82. 35% and 80. 00%,respectively. [Conclusions]This study provides ideas and references for the remediation of heavy metal pollution in paddy soil.

Utilization of Agricultural Waste for Production of Soil Conditioner-Case Study

In the present study,reference is made to a pigsty with an estimated total animal weight of 500,000 kg.The quantities and quality(typical characteristics)of the generated waste are calculated.The total waste occurs after the cleaning washes in the premises of the breeding unit.Semi-liquid waste is treated in solid-liquid phase mechanical separation plants.The resulting solids,waste(reduced humidity),go to storage areas,manure.These areas are shaped concrete basins,in which there is aerobic digestion“composting”.This process results in odorless organochumic material which is further used as a soil conditioner.

Extraction Efficiency of Soil Soluble Nitrogen under Different Conditions

[Objective] The extraction efficiency of soluble nitrogen in common moun- tain red soil under different conditions was analyzed to provide experimental refer- ences for the extraction of soil soluble nitrogen. [Method] The fresh soil and air-dried soil, which were the common mountain red soil under evergreen broad-leaved forest vegetation, were used as the experimental materials. The 2 mol/L KCI and 0.5 mol/L K2SO4 were used as the extractants to extract the soil soluble nitrogen with the extractant-to-soil volume ratio at 4：1, 6：1 and 10：1, respectively, to examine the extrac- tion efficiency of soluble nitrogen in the experimental soil with different extraction conditions. [Result] Compared with K2SO4, the NH4＋-N extracted by KCI was better in quantity and stability; while only at high extractant-to-soil volume ratio could K2SO4 fully extract the soil NH4＋-N. The 2 extractants showed small difference in extracting NO3--N, but at low extractant-to-soil volume ratio, the stability of NO3--N extracted by KCI was higher than that extracted by K2SO4. The properties of NO2- -N extracted by the 2 extractants were very similar. As for soil SON extraction, there was small dif- ference between the 2 extractants. However, the SON extracted by KCI was fewer in quantity than that by K2SO4, but had higher stability, and the extracted SON amount of the 2 extractants showed significant correlation. The extractant-to-soil ratio had little effect on MBN extraction using K2SO4, but the effect was significant when using KCI to extract MBN. The difference between the extraction efficiency of KCI and K^SO4 in extracting fresh soils was greater than that in extracting air-dried soil; therefore, the extractants should be carefully selected in soluble nitrogen extraction from fresh soil. [Conclusion] The results lay the experimental foundation for improving the extraction efficiency of soil soluble nitrogen and increasing the comparability of measurement results.

Differences in Organic C Mineralization Between Aerobic and Submerged Conditions in Paddy Soils of Southern Jiangsu Province,China

Moisture regime plays a crucial role in the mineralization of soil organic carbon （SOC）. In this paper, the dynamics of SOC mineralization in typical paddy soils of Changshu, Jiangsu Province, China, was investigated by incubation test in laboratory. The differences in SOC mineralization under aerobic and submerged conditions of paddy soils were also studied. Results showed that the daily mineralization of SOC under different moisture regimes was significantly different in the whole incubation period, at the beginning of the incubation, it decreased quickly under aerobic condition, but increased rapidly under submerged condition, and both remained constant after 10 d of incubation. The differences in SOC mineralization were found to be mainly at the beginning period of the incubation and decreased along with the incubation time. Thus, the difference was not significantly different at the later incubation period. The respiration intensity, daily and cumulative mineralization of SOC under aerobic condition was 2.26-19.11, 0.96-2.41, and 0.96-2.41 times than those .under submerged condition, respectively. Statistic analyses showed that the higher the contents of microbial biomass carbon and nitrogen, the more significant difference in respiration intensity between aerobic and submerged conditions, but the higher the contents of microbial biomass nitrogen and dissolved organic carbon, the more significant difference in daily mineralization of SOC between the two conditions. The decrease in soil microbial activity under submerged condition was the main reason leading to the decrease in respiration intensity, but the decrease in SOC mineralization was also correlated with the changes in dissolved organic carbon over the whole incubation period.

Biodegradation Kinetics of DDT in Soil under Different Environmental Conditions by Laccase Extract from White Rot Fungi

Biodegradation of 2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane(DDT)in soil by laccase extract from white rot fungi under different experimental conditions was investigated.DDTs,which stands for the sum of p,p′-DDE,o,p′-DDT,p,p′-DDD and p,p′-DDT in soil was degraded efficiently,and the residue decreased rapidly during the first 15 days and then slowly during the period of 16-25 days.The biodegradation of DDTs in soil fitted the pseudo-first-order kinetics.For 5,10,15 and 25 days of incubation with laccase,the residue of DDTs in soil under different atmospheres was decreased by 20%-33%,34%-52%,41%-61%and 41%-69%respectively,under different flooding conditions that was decreased by 12%-17%,17%-30%,30%-45%and 35%-52%respectively, and for different soils that was decreased by 25%-34%,39%-53%,44%-58%and 47%-62%respectively.The half-life of DDTs in soil ranged from 15.07 to 32.95 days under O2,air or N2 atmospheres,23.07 to 40.71 days under different flooding conditions,and 18.78 to 28.88 days for different soils.Laccase is an efficient and safe agent for bioremediation of DDT-contaminated soil.

Seismic fragility of structures isolated by single concave sliding devices for different soil conditions

This study deals with the seismic fragility of elastic structural systems equipped with single concave sliding（friction pendulum system（FPS）） isolators considering different soil conditions. The behavior of these systems is analyzed by employing a two-degree-of-freedom model, whereas the FPS response is described by means of a velocity-dependent model. The uncertainty in the seismic inputs is taken into account by considering artificial seismic excitations modelled as timemodulated filtered Gaussian white noise random processes of different intensity within the power spectral density method. In particular, the filter parameters, which control the frequency content of the random excitations, are calibrated to describe stiff, medium and soft soil conditions. The sliding friction coefficient at large velocity is also considered as a random variable modelled through a uniform probability density function. Incremental dynamic analyses are developed in order to evaluate the probabilities of exceeding different limit states related to both the reinforced concrete（RC） superstructure and isolation level, defining the seismic fragility curves within an extensive parametric study carried out for different structural system properties and soil conditions. The abovementioned seismic fragility curves are useful to evaluate the seismic reliability of base-isolated elastic systems equipped with FPS and located in any site for any soil condition.

Spatial variability of leaf wetness under different soil water conditions in rainfed jujube (Ziziphus jujuba Mill.) in the loess hilly region, China

Leaf wetness provides a wide range of benefits not only to leaves,but also to ecosystems and communities.It regulates canopy eco-hydrological processes and drives spatial differences in hydrological flux.In spite of these functions,little remains known about the spatial distribution of leaf wetness under different soil water conditions.Leaf wetness measurements at the top(180 cm),middle(135 cm),and bottom(85 cm)of the canopy positions of rainfed jujube(Ziziphus jujuba Mill.)in the Chinese loess hilly region were obtained along with meteorological and soil water conditions during the growing seasons in 2019 and 2020.Under soil water non-deficit condition,the frequency of occurrence of leaf wetness was 5.45%higher at the top than at the middle and bottom of the canopy positions.The frequency of occurrence of leaf wetness at the top,middle and bottom of the canopy positions was over 80%at 17:00‒18:00(LST).However,the occurrence of leaf wetness at the top was earlier than those at the middle and bottom of the canopy positions.Correspondingly,leaf drying at the top was also latter than those at the middle and bottom of the canopy positions.Leaf wetness duration at the middle was similar to that at the bottom of the canopy position,but about 1.46-3.01 h less than that at the top.Under soil water deficit condition,the frequency of occurrence of leaf wetness(4.92%-45.45%)followed the order of top>middle>bottom of the canopy position.As the onset of leaf wetness was delayed,the onset of wet leaf drying was advanced and the leaf wetness duration was shortened.Leaf wetness duration at the top was linearly related(R^(2)>0.70)to those at the middle and bottom of the canopy positions under different soil water conditions.In conclusion,the hydrological processes at canopy surfaces of rainfed jujube depended on the position of leaves,thus adjusting canopy structure to redistribute hydrological process is a way to meet the water need of jujube.

Electro-osmotic chemical behavior of clayey soil under various boundary conditions

The use of electro-osmotic chemical is an effective method to improve the clayey soil foundation.Various boundary conditions can be adopted in this method.In this work,two electrode–clay contacts,three solution conditioners,and four anode solution supply times were used for clayey soil improvement.Based on the experimental data,electro-osmotic consolidation theory,and transport of ion theory,it is found that the electro-osmotic chemical effect of the separation of electrode–clay(E_S)is more beneficial for the transport of Ca^(2+),production of cementing material,and reduction of water content than that of electrode–clay(E_C)joining;through electrode–clay contact separation,the anode solution conditioner(NaPO3)6(E_SHMP)delayed the cementing reaction and then increased the transport of Ca^(2+)near the cathode,which increased the amount of cementing material and the electro-osmotic chemical effect;and when the anode conditioner(NaPO3)6 was used,two days of anode solution supply followed by three days cut off from the anode solution led to the highest undrained shear strength increase after the application of electro-osmotic chemical,which resolved the uneven electro-osmotic chemical effect in the E_SHMP.

Dissipation Kinetics of Chlorpyrifos in Soils of a Vegetable Cropping System under Different Cultivation Conditions

Dissipation kinetics of chlorpyrifos in the near-neutral paddy soils of a vegetable cropping system under greenhouse, screenhouse and field conditions was studied using a rapid analytical method. The recoveries of chlorpyrifos were between 86.5% and 105.5% with relative standard deviations for repeatability between 6.6% and 9.1% at fortification levels of 0.01, 0.1 and 1 mg/kg in the soil. The limit of detection of the method was 0.004 mg/kg and the limit of quantification was found to be 0.01 mg/kg. The dissipation rates of chlorpyrifos followed the first-order kinetics and the half-life was 6.96, 6.04 and 5.20 days in the soil under greenhouse, screenhouse and field conditions, respectively. The dissipation rates of chlorpyrifos in the soil varied with different cultivation conditions. Chlorpyrifos in the soil dissipated slower in a greenhouse and screenhouse than in the open field, which was likely attributed to the hermetic environment in the greenhouse and screenhouse.

Study on potential evapotranspiration and wet-dry condition in the seasonal frozen soil region of northern Tibetan Plateau

This study was based on the CEOP/CAMP-Tibet observed data at AWS （Automatic Weather Station） of MS3478 in the seasonal frozen soil region of northern Tibetan Plateau from March 2007 to February 2008. The variation characteristics of PE （potential evapotransph＇ation） were analyzed based on the Penman-Monteith method recommended by FAO （the Food and Agriculture Organization of the United Na- lions）. The contributions of dynamic, thermal and water factors to PE were discussed, and the wet-dry condition of the plateau region was further studied. The results indicated that daily PE was between 0.52 mm and 6.46 mm for the whole year. Monthly PE was over 107 mm from May to September, but decreased to less than 41 mm from November to February. Annual PE was 1,037.8mm. In the summer, thermal PE was significantly more than dynamic PE, but conversely in the winter. Annual variation of thermal PE was of sine wave pattern. In addition, drought and semi-drought climate lasted for a long time while semi-humid climate was short. The effect of water and dynamic factors on PE varied considerably with the seasons. Annual variation of thermal PE was of sine wave pattern.

Optimization of Cellulase Production Conditions in Bacteria Isolated from Soils in Brazzaville

Objective: The objective of the present study is to optimize cellulase production in five strains: (Pantoea dispersa MLTBY6 (MT646430.1);Pseudomonas aeruginosa MLTBM2 (MT646431.1);Pseudomonas monteilii MLTBC10 (MT674682.1);Bacillus subtilis MLTBC5 (MT674681.1) and Lysinibacillus fusiformis MLTBB7 selected cellulase producers isolated from soils in Brazzaville, Republic of the Congo. Materials and Methods: The cellulolytic activity of the selected cellulase-producing strains was determined by transferring the strains to a petri dish containing CMC culture medium with the following composition: cellulose 1%, K2HPO4 0.2%, MgSO4 0.03%, peptone 1%, (NH4)2SO4 0.2% adjusted to a pH value of 7, previously poured and then frozen. The dishes were incubated in an oven at 37°C for 48 hours. The petri dishes were then flooded with 1% lugol for 15 minutes. A positive reading is indicated by the formation of a hydrolysis zone, the diameters of the hydrolysis zone were measured with a ruler. Strains with a broad lysis spectrum were selected. Optimisation of cellulase production by five bacterial strains isolated from the soil was done using the following factors: temperature and pH. Results: The production of cellulase showed that these strains showed a high production of cellulase at pH values between 5.6 and 9 with an optimum of pH = 8 and temperature values between 35°C and 40°C with an optimum at temperature t = 40°C. Of the carbon sources used, two sources, namely glucose and galactose, showed a high production of cellulase compared to the other carbon sources. However, the two nitrogen sources, ammonium sulphate and urea, were favourable for cellulase production by all five strains. Fe2+, CO2+, Zn2+ ions are favourable for cellulase production by these strains, with a referendum for Fe2+. Conclusion: From these results, we conclude that the sources of carbon (glucose and galactose), nitrogen (ammonium sulphate and urea) and ions (Fe2+, CO2+, Zn2+) added to these five strains were the elements favouring the good production of cellulase.